Brevibacterium linens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Brevibacteriaceae; Brevibacterium

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3434 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B8ZX26|A0A0B8ZX26_BRELN Glyoxalase-like domain containing protein OS=Brevibacterium linens OX=1703 GN=AE0388_2888 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84FGAFVVALADD12 pKa = 3.74SVYY15 pKa = 10.11SEE17 pKa = 4.44SMRR20 pKa = 11.84LPLSSIPPRR29 pKa = 11.84WMATAAALGVAVAMSGCSTNEE50 pKa = 3.55ASLTMPVSGTFDD62 pKa = 3.45YY63 pKa = 11.28QLGGTYY69 pKa = 10.45DD70 pKa = 3.84SLPCSVGPSSSVDD83 pKa = 3.41DD84 pKa = 5.28PIDD87 pKa = 3.32IVVRR91 pKa = 11.84DD92 pKa = 4.25AGAAPLTGAYY102 pKa = 8.97SVCYY106 pKa = 10.59VNGFQTQPDD115 pKa = 4.35EE116 pKa = 4.09ATLWRR121 pKa = 11.84DD122 pKa = 2.95HH123 pKa = 7.74DD124 pKa = 4.9DD125 pKa = 4.81LLLHH129 pKa = 6.97DD130 pKa = 5.49ADD132 pKa = 4.74GEE134 pKa = 4.61LVVDD138 pKa = 4.53PDD140 pKa = 3.59WPDD143 pKa = 3.18EE144 pKa = 4.72HH145 pKa = 8.73ILDD148 pKa = 4.53PSTADD153 pKa = 3.33RR154 pKa = 11.84RR155 pKa = 11.84SEE157 pKa = 3.68ILDD160 pKa = 3.56IIGPVIDD167 pKa = 4.52GCASSGFDD175 pKa = 4.08AVEE178 pKa = 3.84IDD180 pKa = 4.3NLDD183 pKa = 3.58TADD186 pKa = 4.47RR187 pKa = 11.84FPDD190 pKa = 3.28IDD192 pKa = 3.53RR193 pKa = 11.84TGALDD198 pKa = 3.99LAAEE202 pKa = 4.44YY203 pKa = 10.82VDD205 pKa = 4.16RR206 pKa = 11.84AHH208 pKa = 7.12AQGLSIAQKK217 pKa = 10.26NAAEE221 pKa = 4.16LAEE224 pKa = 4.34TAHH227 pKa = 6.19SQLSFDD233 pKa = 3.81FAVTEE238 pKa = 4.0EE239 pKa = 4.33CVAFDD244 pKa = 3.19EE245 pKa = 4.82CDD247 pKa = 3.1RR248 pKa = 11.84FTSVYY253 pKa = 9.59GDD255 pKa = 3.47CVLAIEE261 pKa = 4.63YY262 pKa = 9.72PDD264 pKa = 3.69TLVEE268 pKa = 4.51AGLDD272 pKa = 3.63FADD275 pKa = 4.09ACTVPDD281 pKa = 4.82RR282 pKa = 11.84PPLMILRR289 pKa = 11.84DD290 pKa = 3.95RR291 pKa = 11.84DD292 pKa = 3.42LVAAGDD298 pKa = 3.56PGYY301 pKa = 10.96RR302 pKa = 11.84FDD304 pKa = 3.65TCC306 pKa = 5.25

Molecular weight: 32.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B8ZXN5|A0A0B8ZXN5_BRELN Uncharacterized protein OS=Brevibacterium linens OX=1703 GN=AE0388_3108 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 8.99RR13 pKa = 11.84AKK15 pKa = 9.77KK16 pKa = 9.51HH17 pKa = 4.17GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.65GRR40 pKa = 11.84SDD42 pKa = 2.92ISAA45 pKa = 3.67

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3434

0

3434

1150451

33

2097

335.0

35.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.103 ± 0.048

0.614 ± 0.011

6.502 ± 0.048

6.247 ± 0.043

3.406 ± 0.029

8.795 ± 0.038

2.042 ± 0.021

5.133 ± 0.036

2.896 ± 0.036

9.621 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.066 ± 0.018

2.37 ± 0.02

5.08 ± 0.025

2.841 ± 0.026

6.275 ± 0.045

6.436 ± 0.034

6.223 ± 0.026

8.089 ± 0.038

1.351 ± 0.018

1.911 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski