Klebsiella phage Pylas

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Humphriesvirinae; Pylasvirus; Klebsiella virus Pylas

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3BYH2|A0A3G3BYH2_9CAUD Uncharacterized protein OS=Klebsiella phage Pylas OX=2419682 GN=Pylas_011 PE=4 SV=1
MM1 pKa = 7.9DD2 pKa = 3.24IWYY5 pKa = 8.6TEE7 pKa = 4.48LFWSLIGAIVGGITVYY23 pKa = 10.83LAIKK27 pKa = 10.36YY28 pKa = 10.15GDD30 pKa = 3.48NHH32 pKa = 6.85DD33 pKa = 3.74AA34 pKa = 4.59

Molecular weight: 3.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3BZ85|A0A3G3BZ85_9CAUD Uncharacterized protein OS=Klebsiella phage Pylas OX=2419682 GN=Pylas_017 PE=4 SV=1
MM1 pKa = 6.91MRR3 pKa = 11.84KK4 pKa = 9.27SLIQGTKK11 pKa = 10.32ADD13 pKa = 3.63VARR16 pKa = 11.84MKK18 pKa = 10.54KK19 pKa = 9.87RR20 pKa = 11.84EE21 pKa = 4.04LAKK24 pKa = 9.57QTTRR28 pKa = 11.84SDD30 pKa = 2.84NCAPRR35 pKa = 11.84RR36 pKa = 11.84IVTFNHH42 pKa = 6.27PCIQEE47 pKa = 3.93VSS49 pKa = 3.35

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

21581

34

3819

234.6

26.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.016 ± 0.398

1.075 ± 0.209

5.982 ± 0.207

6.547 ± 0.26

3.684 ± 0.173

6.288 ± 0.187

1.701 ± 0.168

6.478 ± 0.204

6.487 ± 0.308

8.646 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.808 ± 0.156

5.56 ± 0.253

3.911 ± 0.145

4.652 ± 0.338

4.402 ± 0.164

6.098 ± 0.21

6.158 ± 0.293

6.71 ± 0.246

1.056 ± 0.12

3.739 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski