Cryobacterium mesophilum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Cryobacterium

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2302 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R8VEJ0|A0A4R8VEJ0_9MICO DNA-binding GntR family transcriptional regulator OS=Cryobacterium mesophilum OX=433647 GN=BKA04_001787 PE=4 SV=1
MM1 pKa = 7.46SISGAPMRR9 pKa = 11.84TSKK12 pKa = 10.58FLSLAAASALLAGGSLLSAGPASASTTVDD41 pKa = 3.03YY42 pKa = 9.2ATFYY46 pKa = 10.36GASDD50 pKa = 3.94FGVEE54 pKa = 4.03SGGYY58 pKa = 5.63PTGVDD63 pKa = 2.96WFFGDD68 pKa = 3.45VSGTEE73 pKa = 4.4GPHH76 pKa = 5.44QFTNTGLVLNDD87 pKa = 3.86PASGDD92 pKa = 3.77VQILNQNVTTPVDD105 pKa = 3.6AGEE108 pKa = 4.25LASVVQNANIFAQNGEE124 pKa = 4.3WFFQLAFFAEE134 pKa = 5.05GTSDD138 pKa = 3.37TGFTTLRR145 pKa = 11.84PATAGSVSTGDD156 pKa = 3.26PWITSQALGSYY167 pKa = 9.94AAGATASFSDD177 pKa = 4.91LADD180 pKa = 3.4ALYY183 pKa = 10.71AGEE186 pKa = 4.64APQLLAYY193 pKa = 10.18GFFVAAGQTTTINGISWGTQGSSFGLPPTRR223 pKa = 11.84TISPNPITQDD233 pKa = 3.08AFSTAGQGLTLSGTNWFPGVDD254 pKa = 3.39AYY256 pKa = 10.26IFIVDD261 pKa = 3.84HH262 pKa = 6.59NGTTVFEE269 pKa = 4.44DD270 pKa = 3.63TSSFIVDD277 pKa = 3.3SAGNVSVSVVLPSKK291 pKa = 10.36PDD293 pKa = 3.0VGTYY297 pKa = 10.38YY298 pKa = 9.63VTFDD302 pKa = 4.2DD303 pKa = 5.28DD304 pKa = 3.56SFYY307 pKa = 11.51YY308 pKa = 8.7HH309 pKa = 6.79TGVLDD314 pKa = 4.25HH315 pKa = 6.5YY316 pKa = 11.09VSAPGGGEE324 pKa = 4.17GEE326 pKa = 4.34GSNAQGIQISVIAAAPEE343 pKa = 3.85LAATGAEE350 pKa = 4.22SGTLIAGGILLLILGGVVLIGSRR373 pKa = 11.84RR374 pKa = 11.84RR375 pKa = 11.84RR376 pKa = 11.84AQLGG380 pKa = 3.42

Molecular weight: 38.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V3I9Q4|A0A4V3I9Q4_9MICO Ribonucleoside-diphosphate reductase OS=Cryobacterium mesophilum OX=433647 GN=BKA04_001889 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2302

0

2302

748348

22

1971

325.1

34.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.628 ± 0.069

0.511 ± 0.013

6.103 ± 0.042

5.511 ± 0.046

3.316 ± 0.026

8.932 ± 0.04

1.963 ± 0.026

5.107 ± 0.039

2.441 ± 0.038

10.253 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.859 ± 0.019

2.268 ± 0.027

5.192 ± 0.036

2.716 ± 0.026

6.849 ± 0.049

6.127 ± 0.038

5.969 ± 0.038

8.787 ± 0.049

1.444 ± 0.018

2.025 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski