Marinobacter similis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Marinobacter

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2692 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5YMT3|W5YMT3_9ALTE LysR family transcriptional regulator OS=Marinobacter similis OX=1420916 GN=AU14_01415 PE=3 SV=1
MM1 pKa = 7.43SVFKK5 pKa = 10.97ALGLGSALILLGGCQSWRR23 pKa = 11.84FQTVEE28 pKa = 4.25EE29 pKa = 4.71LPPPAAEE36 pKa = 4.9PEE38 pKa = 4.24TSQPGEE44 pKa = 4.13VEE46 pKa = 3.65VRR48 pKa = 11.84YY49 pKa = 10.23YY50 pKa = 11.49DD51 pKa = 3.7NLDD54 pKa = 3.46GEE56 pKa = 5.37EE57 pKa = 4.43IDD59 pKa = 5.0TMTTSAKK66 pKa = 10.22FPSNPDD72 pKa = 2.85SVASLNSLEE81 pKa = 4.85DD82 pKa = 3.66PEE84 pKa = 5.45NRR86 pKa = 11.84AEE88 pKa = 4.62DD89 pKa = 3.51YY90 pKa = 10.95GSYY93 pKa = 10.72VRR95 pKa = 11.84GFIKK99 pKa = 10.52PPTSGEE105 pKa = 3.71YY106 pKa = 10.44RR107 pKa = 11.84FFISGNDD114 pKa = 3.28QAEE117 pKa = 4.56FWLSTSDD124 pKa = 3.57SPEE127 pKa = 4.1NVDD130 pKa = 5.11LMAMTPAWTSINEE143 pKa = 3.72FDD145 pKa = 4.62KK146 pKa = 11.51YY147 pKa = 11.05SSQTSRR153 pKa = 11.84YY154 pKa = 7.45ITLDD158 pKa = 2.95SSKK161 pKa = 10.55RR162 pKa = 11.84YY163 pKa = 9.48YY164 pKa = 10.88FEE166 pKa = 4.5VLQKK170 pKa = 10.79EE171 pKa = 5.04GFGSDD176 pKa = 3.01HH177 pKa = 6.88FAVAWEE183 pKa = 4.74GPGISQEE190 pKa = 4.51VIGSSYY196 pKa = 10.0IYY198 pKa = 10.38SYY200 pKa = 11.4AKK202 pKa = 9.87PAIDD206 pKa = 3.4TGQTGQEE213 pKa = 4.18AYY215 pKa = 10.8NLGYY219 pKa = 10.26RR220 pKa = 11.84VGYY223 pKa = 10.47VDD225 pKa = 3.51GTEE228 pKa = 3.97GLSFNPAFPPADD240 pKa = 3.42QDD242 pKa = 3.67QDD244 pKa = 3.95GIYY247 pKa = 10.68DD248 pKa = 3.26NWEE251 pKa = 4.05VVNGLNPNDD260 pKa = 4.39SNDD263 pKa = 3.55ATSDD267 pKa = 3.64PDD269 pKa = 3.93GDD271 pKa = 4.19LLAAADD277 pKa = 4.07EE278 pKa = 4.38FLIGTSEE285 pKa = 3.93NTADD289 pKa = 4.05SDD291 pKa = 4.51GDD293 pKa = 4.17GIPDD297 pKa = 3.32GVEE300 pKa = 3.91FANEE304 pKa = 3.99LDD306 pKa = 3.71PLYY309 pKa = 10.87KK310 pKa = 9.79QDD312 pKa = 4.13AQEE315 pKa = 5.24DD316 pKa = 4.34LDD318 pKa = 4.0NDD320 pKa = 4.33GYY322 pKa = 11.78SNLEE326 pKa = 3.99EE327 pKa = 4.35YY328 pKa = 10.81VAGTVINDD336 pKa = 3.71PDD338 pKa = 4.49DD339 pKa = 4.88APVAAPSPEE348 pKa = 4.25PEE350 pKa = 4.59PEE352 pKa = 4.22PEE354 pKa = 4.48PEE356 pKa = 4.27PEE358 pKa = 4.09PTPSPTEE365 pKa = 3.79EE366 pKa = 3.81PSYY369 pKa = 10.72VAGFGAQFFEE379 pKa = 4.34GTSFNRR385 pKa = 11.84FVLTDD390 pKa = 2.81VHH392 pKa = 6.14EE393 pKa = 4.61AVNFDD398 pKa = 3.45WGRR401 pKa = 11.84GQPLAEE407 pKa = 4.37LPEE410 pKa = 4.5DD411 pKa = 3.36QFSIRR416 pKa = 11.84WNGIFTAPHH425 pKa = 5.38QSGDD429 pKa = 2.8NDD431 pKa = 3.34YY432 pKa = 11.14RR433 pKa = 11.84FTVSTNDD440 pKa = 3.39GVRR443 pKa = 11.84MFVNGEE449 pKa = 3.98LVIDD453 pKa = 4.55DD454 pKa = 4.0WSEE457 pKa = 4.15HH458 pKa = 5.58PTTTYY463 pKa = 10.24TYY465 pKa = 10.72DD466 pKa = 3.35RR467 pKa = 11.84SLAAGEE473 pKa = 4.32KK474 pKa = 10.27LSLTIEE480 pKa = 4.25YY481 pKa = 10.85FEE483 pKa = 4.59GTGSAVAQYY492 pKa = 10.99SATNLTSAEE501 pKa = 4.1NLSTRR506 pKa = 11.84ATVATPDD513 pKa = 3.72PSTSNTLDD521 pKa = 3.41SDD523 pKa = 3.8NDD525 pKa = 4.73GIPDD529 pKa = 3.32TWEE532 pKa = 3.87LRR534 pKa = 11.84NGTSVWVPDD543 pKa = 3.5GGVVNNSQGTSNVEE557 pKa = 3.74AYY559 pKa = 9.98QAGLTPYY566 pKa = 9.71TLATVEE572 pKa = 4.23TDD574 pKa = 3.56GKK576 pKa = 7.76PTSGSGSDD584 pKa = 3.54TGDD587 pKa = 2.93TTTPDD592 pKa = 3.53EE593 pKa = 4.78PSTTEE598 pKa = 3.64PAPPEE603 pKa = 4.26TGSGSVTLTWTAPLTRR619 pKa = 11.84LDD621 pKa = 3.79GSSIALSEE629 pKa = 4.13IASYY633 pKa = 9.77TIDD636 pKa = 3.4YY637 pKa = 9.62GQDD640 pKa = 3.16VNNLEE645 pKa = 4.33EE646 pKa = 4.36SVSVGGDD653 pKa = 3.09QTSYY657 pKa = 11.61SFDD660 pKa = 3.46NLASGTWYY668 pKa = 10.56FKK670 pKa = 10.88IEE672 pKa = 4.14VVDD675 pKa = 3.95TNGLSSPPSNPVSTQVQQ692 pKa = 3.28

Molecular weight: 74.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5YIL3|W5YIL3_9ALTE 3-hydroxyacyl-CoA dehydrogenase OS=Marinobacter similis OX=1420916 GN=AU14_10575 PE=3 SV=1
MM1 pKa = 7.91DD2 pKa = 3.74WMKK5 pKa = 10.86EE6 pKa = 3.59LAALPKK12 pKa = 9.44TLRR15 pKa = 11.84ATLIAALVLLVALILVNQPLQTGSAPQGIVSFQMAGTADD54 pKa = 3.39QAHH57 pKa = 7.27AILRR61 pKa = 11.84SWRR64 pKa = 11.84SGGEE68 pKa = 3.72LWAHH72 pKa = 6.18ISLWLDD78 pKa = 3.11FLFIPAYY85 pKa = 9.89VLALIQLTRR94 pKa = 11.84HH95 pKa = 5.58FTRR98 pKa = 11.84DD99 pKa = 3.06RR100 pKa = 11.84PGVRR104 pKa = 11.84EE105 pKa = 3.84RR106 pKa = 11.84MVARR110 pKa = 11.84WIRR113 pKa = 11.84ALFVAAGLSDD123 pKa = 3.01ITEE126 pKa = 4.65NILLLNNFNPPTDD139 pKa = 3.8VVSLSATICALIKK152 pKa = 10.09YY153 pKa = 7.44TGLTLGIAGLVIVRR167 pKa = 11.84ASRR170 pKa = 11.84RR171 pKa = 11.84HH172 pKa = 5.36PLAHH176 pKa = 6.6GG177 pKa = 3.74

Molecular weight: 19.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2692

0

2692

735979

30

1651

273.4

30.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.861 ± 0.053

0.927 ± 0.017

5.841 ± 0.041

6.547 ± 0.049

3.819 ± 0.037

7.738 ± 0.042

2.137 ± 0.026

5.158 ± 0.042

3.674 ± 0.043

10.626 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.583 ± 0.024

3.313 ± 0.03

4.617 ± 0.029

4.147 ± 0.038

6.319 ± 0.047

6.198 ± 0.038

5.242 ± 0.042

7.391 ± 0.035

1.347 ± 0.022

2.514 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski