Pontibacter akesuensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Pontibacter

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4073 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I7GRK5|A0A1I7GRK5_9BACT Peptidase family M28 OS=Pontibacter akesuensis OX=388950 GN=SAMN04487941_1211 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.45KK3 pKa = 10.22KK4 pKa = 10.45LLPLRR9 pKa = 11.84LLSFLVCLFFSTAAMAQQVFINEE32 pKa = 3.48IHH34 pKa = 6.59YY35 pKa = 11.24DD36 pKa = 3.57NDD38 pKa = 3.81GTDD41 pKa = 2.88SGEE44 pKa = 4.16AVEE47 pKa = 4.79IAAPSGTDD55 pKa = 3.22LTGWSLVLYY64 pKa = 10.07NGSNNEE70 pKa = 4.15TYY72 pKa = 8.73QTIPLTGAVPASSISYY88 pKa = 10.41GFLSVNTPGIQNGSPDD104 pKa = 4.34AIALVNAAGEE114 pKa = 4.57VVQFLSYY121 pKa = 10.77EE122 pKa = 4.24GTLTAVNGPASGMTSEE138 pKa = 5.46DD139 pKa = 2.92IGVSEE144 pKa = 5.44SSTTPAGYY152 pKa = 10.3SLQLGNIDD160 pKa = 5.23DD161 pKa = 4.88GNASADD167 pKa = 3.92FTWQEE172 pKa = 3.84AATSTFGEE180 pKa = 4.27INNNQVFPLGGGTPDD195 pKa = 4.44PEE197 pKa = 4.59PEE199 pKa = 4.43PEE201 pKa = 4.58PEE203 pKa = 4.27PEE205 pKa = 3.8PAITLVFINEE215 pKa = 3.83LHH217 pKa = 6.51YY218 pKa = 11.53DD219 pKa = 3.63NASTDD224 pKa = 3.17TGEE227 pKa = 4.29GVEE230 pKa = 4.21IAGLAGTNLEE240 pKa = 3.97GWQLVGYY247 pKa = 9.3NGNGGANYY255 pKa = 8.11STTSLSGVIPNQQSGFGTVFFPIPGLQNGSPDD287 pKa = 3.94GIALVNSEE295 pKa = 4.07GQVVQFLSYY304 pKa = 10.08EE305 pKa = 4.09GAFAAVGGPADD316 pKa = 3.5GMMSEE321 pKa = 5.15DD322 pKa = 3.37IGVAEE327 pKa = 5.36AGDD330 pKa = 4.13SPTGYY335 pKa = 10.55SLQLTGSGSSYY346 pKa = 11.5DD347 pKa = 3.55AFTWAASSTATYY359 pKa = 11.06DD360 pKa = 3.43EE361 pKa = 4.69VNNGQTFLPLQDD373 pKa = 3.22VVFINEE379 pKa = 3.68LHH381 pKa = 6.3YY382 pKa = 11.54DD383 pKa = 3.53NDD385 pKa = 4.08GADD388 pKa = 3.08AGEE391 pKa = 4.28GVEE394 pKa = 4.1IAGNAGADD402 pKa = 3.47LSGWQLVLYY411 pKa = 9.68NGNGGTAYY419 pKa = 10.55SALEE423 pKa = 3.98LSGTIPNQDD432 pKa = 2.95NGYY435 pKa = 8.12GTLFFAITGLQNGAPDD451 pKa = 4.36GVALINPDD459 pKa = 3.53GEE461 pKa = 4.64VVQFLSYY468 pKa = 10.34EE469 pKa = 4.24GSFTATDD476 pKa = 3.59GLAAGVTSEE485 pKa = 5.23DD486 pKa = 3.1IGVMEE491 pKa = 5.31SSSTPLNYY499 pKa = 10.22SLQLTGTGTSYY510 pKa = 12.05ADD512 pKa = 3.83FTWGGPITSTYY523 pKa = 11.21NLINTGQSFGGSTQEE538 pKa = 4.4PEE540 pKa = 3.96PEE542 pKa = 4.16VPVEE546 pKa = 3.85GSIAEE551 pKa = 4.11ARR553 pKa = 11.84TLPVGTVVTVSGVLTATNQFGGPAYY578 pKa = 10.5LQDD581 pKa = 3.62ATAGIAVFDD590 pKa = 4.36PQVHH594 pKa = 6.46GDD596 pKa = 3.4GAFAIGDD603 pKa = 4.36SIQITASVEE612 pKa = 4.03LFNQMPEE619 pKa = 4.15LVNITSIEE627 pKa = 4.22SFGPATTTLAPITTTIADD645 pKa = 4.05LNQYY649 pKa = 10.6EE650 pKa = 4.54GMLVTIPGISFTDD663 pKa = 3.71PEE665 pKa = 4.56GLLFPDD671 pKa = 3.73SNYY674 pKa = 10.57EE675 pKa = 3.89ITDD678 pKa = 3.42GTGTVALRR686 pKa = 11.84IDD688 pKa = 4.18GDD690 pKa = 3.85VEE692 pKa = 4.42DD693 pKa = 4.23LVGRR697 pKa = 11.84LKK699 pKa = 10.52PQEE702 pKa = 4.22PVTITGIVSSFRR714 pKa = 11.84GSLQLLPRR722 pKa = 11.84FMADD726 pKa = 3.15LPGTEE731 pKa = 5.23AYY733 pKa = 9.81VQAGSDD739 pKa = 3.32IPVAQTLDD747 pKa = 3.52VMSWNMEE754 pKa = 4.11FFGATQKK761 pKa = 11.25DD762 pKa = 3.76FGPNNEE768 pKa = 4.43LLQLQNAAEE777 pKa = 4.34LLDD780 pKa = 4.48SIRR783 pKa = 11.84ADD785 pKa = 3.36IIAVQEE791 pKa = 4.23VSDD794 pKa = 4.06EE795 pKa = 4.2NQLQQLVDD803 pKa = 3.81MLPGYY808 pKa = 10.88ARR810 pKa = 11.84ICSDD814 pKa = 3.47RR815 pKa = 11.84YY816 pKa = 9.57SYY818 pKa = 11.4SFNGEE823 pKa = 4.01DD824 pKa = 4.38PNFPAQKK831 pKa = 10.47LCFIYY836 pKa = 9.03NTEE839 pKa = 3.94VVTVVDD845 pKa = 3.72EE846 pKa = 4.39RR847 pKa = 11.84VLFEE851 pKa = 4.36EE852 pKa = 5.7LYY854 pKa = 10.31DD855 pKa = 3.74AARR858 pKa = 11.84AGQTDD863 pKa = 4.37LLNNYY868 pKa = 8.99PSGEE872 pKa = 3.94ASSFWSSGRR881 pKa = 11.84LPYY884 pKa = 9.82MVTVDD889 pKa = 3.28ATINGVTEE897 pKa = 5.21RR898 pKa = 11.84IQLINIHH905 pKa = 6.35AKK907 pKa = 10.3SGSNNNDD914 pKa = 3.44LARR917 pKa = 11.84RR918 pKa = 11.84AYY920 pKa = 9.96DD921 pKa = 3.47VQVLKK926 pKa = 11.01DD927 pKa = 3.7SLDD930 pKa = 3.35THH932 pKa = 6.26YY933 pKa = 11.83ANANLILLGDD943 pKa = 3.98YY944 pKa = 11.03NDD946 pKa = 5.54DD947 pKa = 3.25VDD949 pKa = 4.67ASIGSGEE956 pKa = 4.14TVYY959 pKa = 11.02QLFVQDD965 pKa = 2.78IDD967 pKa = 4.39YY968 pKa = 11.35DD969 pKa = 4.23VVTLSLSEE977 pKa = 4.23AGLRR981 pKa = 11.84SYY983 pKa = 8.94ITQEE987 pKa = 3.66NVIDD991 pKa = 5.23HH992 pKa = 5.93ITISDD997 pKa = 3.81EE998 pKa = 4.15LYY1000 pKa = 9.89EE1001 pKa = 4.16EE1002 pKa = 4.67YY1003 pKa = 10.34IEE1005 pKa = 4.61GSEE1008 pKa = 3.92RR1009 pKa = 11.84LIIPFSYY1016 pKa = 10.21IEE1018 pKa = 4.26NYY1020 pKa = 10.85GNTTSDD1026 pKa = 3.52HH1027 pKa = 6.59LPVLVRR1033 pKa = 11.84FEE1035 pKa = 4.23LTAPAPLVVDD1045 pKa = 4.44AGTNQTVYY1053 pKa = 10.5FGYY1056 pKa = 10.65APQACATLTASTPTDD1071 pKa = 3.3GSGMYY1076 pKa = 9.78TYY1078 pKa = 9.35TWSNGQTGQSIQVCPDD1094 pKa = 2.71EE1095 pKa = 4.5TTTYY1099 pKa = 9.72TVTVTDD1105 pKa = 3.67SNGRR1109 pKa = 11.84TGTDD1113 pKa = 3.12EE1114 pKa = 4.29VQVCVVNVVCTKK1126 pKa = 9.96NARR1129 pKa = 11.84TPMVEE1134 pKa = 3.42ICFRR1138 pKa = 11.84PPGRR1142 pKa = 11.84SMEE1145 pKa = 4.31SARR1148 pKa = 11.84TLCVPVQAVEE1158 pKa = 4.18PMLKK1162 pKa = 10.43LGATLGSCGTVACGEE1177 pKa = 4.35EE1178 pKa = 4.4AVEE1181 pKa = 4.74PAPSMPFSITVYY1193 pKa = 10.38PNPLKK1198 pKa = 10.92DD1199 pKa = 3.45FAEE1202 pKa = 4.35VKK1204 pKa = 10.3IEE1206 pKa = 3.8GMVNGDD1212 pKa = 3.88VQVVLYY1218 pKa = 10.26DD1219 pKa = 3.5HH1220 pKa = 7.23FSNEE1224 pKa = 4.01VLSKK1228 pKa = 10.79QISTKK1233 pKa = 10.73DD1234 pKa = 3.36GSFKK1238 pKa = 11.02LDD1240 pKa = 3.6LNSNRR1245 pKa = 11.84LKK1247 pKa = 11.03AGLYY1251 pKa = 7.24YY1252 pKa = 10.8LKK1254 pKa = 10.7VISQNNSQTVRR1265 pKa = 11.84IIKK1268 pKa = 9.55QQ1269 pKa = 2.98

Molecular weight: 134.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I7J0S6|A0A1I7J0S6_9BACT Quinol:cytochrome c oxidoreductase monoheme cytochrome subunit OS=Pontibacter akesuensis OX=388950 GN=SAMN04487941_2382 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.54RR3 pKa = 11.84TFQPSQRR10 pKa = 11.84KK11 pKa = 8.72RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.56HH16 pKa = 3.97GFRR19 pKa = 11.84SRR21 pKa = 11.84MEE23 pKa = 3.95TANGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.54GRR39 pKa = 11.84KK40 pKa = 8.64RR41 pKa = 11.84LTVSSEE47 pKa = 3.88KK48 pKa = 9.99RR49 pKa = 11.84HH50 pKa = 5.45KK51 pKa = 10.57AAA53 pKa = 5.14

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4073

0

4073

1372960

38

3596

337.1

37.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.392 ± 0.04

0.712 ± 0.013

5.047 ± 0.027

6.512 ± 0.047

4.483 ± 0.028

6.989 ± 0.043

2.0 ± 0.022

5.894 ± 0.034

5.838 ± 0.042

10.165 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.41 ± 0.019

4.681 ± 0.029

4.137 ± 0.025

4.383 ± 0.03

4.6 ± 0.029

5.947 ± 0.035

5.674 ± 0.048

7.038 ± 0.031

1.138 ± 0.017

3.961 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski