Amino acid dipepetide frequency for Mycobacterium phage Phasih

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.118AlaAla: 13.118 ± 1.819
0.617AlaCys: 0.617 ± 0.199
7.064AlaAsp: 7.064 ± 0.565
7.232AlaGlu: 7.232 ± 0.68
2.411AlaPhe: 2.411 ± 0.365
10.203AlaGly: 10.203 ± 1.297
2.242AlaHis: 2.242 ± 0.407
4.205AlaIle: 4.205 ± 0.561
4.485AlaLys: 4.485 ± 0.522
8.297AlaLeu: 8.297 ± 0.83
2.242AlaMet: 2.242 ± 0.382
2.915AlaAsn: 2.915 ± 0.439
4.989AlaPro: 4.989 ± 0.604
3.027AlaGln: 3.027 ± 0.488
6.951AlaArg: 6.951 ± 0.757
5.158AlaSer: 5.158 ± 0.575
6.223AlaThr: 6.223 ± 0.573
6.951AlaVal: 6.951 ± 0.695
2.242AlaTrp: 2.242 ± 0.326
2.13AlaTyr: 2.13 ± 0.339
0.0AlaXaa: 0.0 ± 0.0
Cys
1.065CysAla: 1.065 ± 0.294
0.168CysCys: 0.168 ± 0.092
1.345CysAsp: 1.345 ± 0.337
0.897CysGlu: 0.897 ± 0.239
0.336CysPhe: 0.336 ± 0.168
1.514CysGly: 1.514 ± 0.341
0.28CysHis: 0.28 ± 0.128
0.112CysIle: 0.112 ± 0.088
0.617CysLys: 0.617 ± 0.197
0.617CysLeu: 0.617 ± 0.212
0.224CysMet: 0.224 ± 0.104
0.448CysAsn: 0.448 ± 0.137
1.402CysPro: 1.402 ± 0.321
0.336CysGln: 0.336 ± 0.163
0.841CysArg: 0.841 ± 0.237
0.841CysSer: 0.841 ± 0.266
0.953CysThr: 0.953 ± 0.271
0.673CysVal: 0.673 ± 0.16
0.448CysTrp: 0.448 ± 0.158
0.28CysTyr: 0.28 ± 0.128
0.0CysXaa: 0.0 ± 0.0
Asp
6.503AspAla: 6.503 ± 0.667
0.785AspCys: 0.785 ± 0.202
4.429AspAsp: 4.429 ± 0.503
3.588AspGlu: 3.588 ± 0.473
1.626AspPhe: 1.626 ± 0.287
6.223AspGly: 6.223 ± 0.603
1.514AspHis: 1.514 ± 0.328
2.467AspIle: 2.467 ± 0.375
1.738AspLys: 1.738 ± 0.264
5.83AspLeu: 5.83 ± 0.515
1.233AspMet: 1.233 ± 0.284
1.794AspAsn: 1.794 ± 0.334
4.765AspPro: 4.765 ± 0.637
2.635AspGln: 2.635 ± 0.418
5.158AspArg: 5.158 ± 0.759
3.251AspSer: 3.251 ± 0.485
4.373AspThr: 4.373 ± 0.535
4.485AspVal: 4.485 ± 0.538
1.402AspTrp: 1.402 ± 0.302
2.242AspTyr: 2.242 ± 0.369
0.0AspXaa: 0.0 ± 0.0
Glu
5.718GluAla: 5.718 ± 0.684
1.121GluCys: 1.121 ± 0.233
3.083GluAsp: 3.083 ± 0.357
2.803GluGlu: 2.803 ± 0.52
2.186GluPhe: 2.186 ± 0.324
3.588GluGly: 3.588 ± 0.473
1.906GluHis: 1.906 ± 0.396
2.074GluIle: 2.074 ± 0.365
1.962GluLys: 1.962 ± 0.307
5.998GluLeu: 5.998 ± 0.576
1.402GluMet: 1.402 ± 0.318
1.962GluAsn: 1.962 ± 0.272
2.915GluPro: 2.915 ± 0.354
2.915GluGln: 2.915 ± 0.434
5.27GluArg: 5.27 ± 0.677
3.083GluSer: 3.083 ± 0.407
4.317GluThr: 4.317 ± 0.577
3.812GluVal: 3.812 ± 0.511
1.57GluTrp: 1.57 ± 0.264
1.906GluTyr: 1.906 ± 0.368
0.0GluXaa: 0.0 ± 0.0
Phe
2.915PheAla: 2.915 ± 0.384
0.336PheCys: 0.336 ± 0.131
2.635PheAsp: 2.635 ± 0.368
1.626PheGlu: 1.626 ± 0.325
0.953PhePhe: 0.953 ± 0.292
2.467PheGly: 2.467 ± 0.591
0.561PheHis: 0.561 ± 0.181
1.402PheIle: 1.402 ± 0.328
1.121PheLys: 1.121 ± 0.271
1.458PheLeu: 1.458 ± 0.291
0.729PheMet: 0.729 ± 0.189
1.402PheAsn: 1.402 ± 0.339
1.514PhePro: 1.514 ± 0.308
1.289PheGln: 1.289 ± 0.359
1.345PheArg: 1.345 ± 0.273
1.402PheSer: 1.402 ± 0.303
2.186PheThr: 2.186 ± 0.353
1.906PheVal: 1.906 ± 0.263
0.561PheTrp: 0.561 ± 0.142
0.729PheTyr: 0.729 ± 0.268
0.0PheXaa: 0.0 ± 0.0
Gly
8.857GlyAla: 8.857 ± 1.036
1.57GlyCys: 1.57 ± 0.328
5.774GlyAsp: 5.774 ± 0.494
4.148GlyGlu: 4.148 ± 0.541
2.523GlyPhe: 2.523 ± 0.447
10.707GlyGly: 10.707 ± 2.083
1.906GlyHis: 1.906 ± 0.35
3.756GlyIle: 3.756 ± 0.458
2.411GlyLys: 2.411 ± 0.33
5.83GlyLeu: 5.83 ± 0.571
2.242GlyMet: 2.242 ± 0.365
3.139GlyAsn: 3.139 ± 0.348
3.98GlyPro: 3.98 ± 0.532
2.186GlyGln: 2.186 ± 0.514
5.214GlyArg: 5.214 ± 0.677
6.167GlySer: 6.167 ± 0.849
6.279GlyThr: 6.279 ± 0.708
6.167GlyVal: 6.167 ± 0.579
2.523GlyTrp: 2.523 ± 0.355
2.018GlyTyr: 2.018 ± 0.417
0.0GlyXaa: 0.0 ± 0.0
His
1.514HisAla: 1.514 ± 0.349
0.392HisCys: 0.392 ± 0.191
1.289HisAsp: 1.289 ± 0.26
1.402HisGlu: 1.402 ± 0.299
0.561HisPhe: 0.561 ± 0.16
1.85HisGly: 1.85 ± 0.297
0.953HisHis: 0.953 ± 0.29
1.738HisIle: 1.738 ± 0.32
0.953HisLys: 0.953 ± 0.253
1.906HisLeu: 1.906 ± 0.36
0.448HisMet: 0.448 ± 0.129
1.065HisAsn: 1.065 ± 0.248
1.402HisPro: 1.402 ± 0.248
0.729HisGln: 0.729 ± 0.174
2.074HisArg: 2.074 ± 0.458
0.953HisSer: 0.953 ± 0.247
1.626HisThr: 1.626 ± 0.39
1.289HisVal: 1.289 ± 0.296
0.448HisTrp: 0.448 ± 0.153
0.953HisTyr: 0.953 ± 0.236
0.0HisXaa: 0.0 ± 0.0
Ile
4.989IleAla: 4.989 ± 0.581
0.673IleCys: 0.673 ± 0.187
3.476IleAsp: 3.476 ± 0.494
3.42IleGlu: 3.42 ± 0.407
0.561IlePhe: 0.561 ± 0.216
4.036IleGly: 4.036 ± 0.464
1.57IleHis: 1.57 ± 0.291
1.458IleIle: 1.458 ± 0.287
1.233IleLys: 1.233 ± 0.255
2.355IleLeu: 2.355 ± 0.341
0.28IleMet: 0.28 ± 0.125
2.186IleAsn: 2.186 ± 0.304
2.579IlePro: 2.579 ± 0.358
1.458IleGln: 1.458 ± 0.291
2.579IleArg: 2.579 ± 0.366
2.298IleSer: 2.298 ± 0.379
3.195IleThr: 3.195 ± 0.351
3.42IleVal: 3.42 ± 0.384
1.009IleTrp: 1.009 ± 0.269
0.897IleTyr: 0.897 ± 0.251
0.0IleXaa: 0.0 ± 0.0
Lys
3.924LysAla: 3.924 ± 0.586
0.448LysCys: 0.448 ± 0.151
1.906LysAsp: 1.906 ± 0.329
1.289LysGlu: 1.289 ± 0.243
1.233LysPhe: 1.233 ± 0.219
2.242LysGly: 2.242 ± 0.339
0.953LysHis: 0.953 ± 0.213
1.289LysIle: 1.289 ± 0.242
1.345LysLys: 1.345 ± 0.363
2.635LysLeu: 2.635 ± 0.522
0.729LysMet: 0.729 ± 0.195
0.897LysAsn: 0.897 ± 0.247
2.355LysPro: 2.355 ± 0.38
1.682LysGln: 1.682 ± 0.243
2.747LysArg: 2.747 ± 0.411
2.018LysSer: 2.018 ± 0.349
2.242LysThr: 2.242 ± 0.362
2.523LysVal: 2.523 ± 0.394
1.009LysTrp: 1.009 ± 0.248
1.233LysTyr: 1.233 ± 0.307
0.0LysXaa: 0.0 ± 0.0
Leu
8.073LeuAla: 8.073 ± 1.069
1.009LeuCys: 1.009 ± 0.291
4.373LeuAsp: 4.373 ± 0.561
3.924LeuGlu: 3.924 ± 0.4
2.355LeuPhe: 2.355 ± 0.31
5.045LeuGly: 5.045 ± 0.606
1.121LeuHis: 1.121 ± 0.268
2.859LeuIle: 2.859 ± 0.413
1.962LeuLys: 1.962 ± 0.327
4.933LeuLeu: 4.933 ± 0.505
1.85LeuMet: 1.85 ± 0.344
2.467LeuAsn: 2.467 ± 0.375
5.662LeuPro: 5.662 ± 0.684
2.691LeuGln: 2.691 ± 0.42
5.326LeuArg: 5.326 ± 0.595
5.101LeuSer: 5.101 ± 0.548
5.494LeuThr: 5.494 ± 0.463
5.214LeuVal: 5.214 ± 0.572
0.897LeuTrp: 0.897 ± 0.22
2.298LeuTyr: 2.298 ± 0.369
0.0LeuXaa: 0.0 ± 0.0
Met
2.186MetAla: 2.186 ± 0.348
0.336MetCys: 0.336 ± 0.196
1.121MetAsp: 1.121 ± 0.268
1.233MetGlu: 1.233 ± 0.225
0.729MetPhe: 0.729 ± 0.225
1.794MetGly: 1.794 ± 0.284
0.168MetHis: 0.168 ± 0.103
0.897MetIle: 0.897 ± 0.252
0.841MetLys: 0.841 ± 0.244
1.626MetLeu: 1.626 ± 0.24
0.392MetMet: 0.392 ± 0.223
0.673MetAsn: 0.673 ± 0.195
1.458MetPro: 1.458 ± 0.266
0.561MetGln: 0.561 ± 0.165
1.57MetArg: 1.57 ± 0.278
2.579MetSer: 2.579 ± 0.389
2.186MetThr: 2.186 ± 0.318
1.458MetVal: 1.458 ± 0.353
0.168MetTrp: 0.168 ± 0.095
0.336MetTyr: 0.336 ± 0.156
0.0MetXaa: 0.0 ± 0.0
Asn
3.588AsnAla: 3.588 ± 0.368
0.336AsnCys: 0.336 ± 0.12
2.074AsnAsp: 2.074 ± 0.346
1.85AsnGlu: 1.85 ± 0.381
0.841AsnPhe: 0.841 ± 0.303
4.036AsnGly: 4.036 ± 0.62
1.121AsnHis: 1.121 ± 0.238
1.682AsnIle: 1.682 ± 0.45
1.177AsnLys: 1.177 ± 0.265
2.186AsnLeu: 2.186 ± 0.426
0.617AsnMet: 0.617 ± 0.15
1.794AsnAsn: 1.794 ± 0.375
2.635AsnPro: 2.635 ± 0.42
1.009AsnGln: 1.009 ± 0.312
2.523AsnArg: 2.523 ± 0.459
1.626AsnSer: 1.626 ± 0.282
2.186AsnThr: 2.186 ± 0.365
1.85AsnVal: 1.85 ± 0.379
0.729AsnTrp: 0.729 ± 0.203
0.673AsnTyr: 0.673 ± 0.184
0.0AsnXaa: 0.0 ± 0.0
Pro
5.214ProAla: 5.214 ± 0.561
0.617ProCys: 0.617 ± 0.185
4.541ProAsp: 4.541 ± 0.43
4.709ProGlu: 4.709 ± 0.421
1.682ProPhe: 1.682 ± 0.32
6.391ProGly: 6.391 ± 0.666
1.458ProHis: 1.458 ± 0.299
1.85ProIle: 1.85 ± 0.318
2.355ProLys: 2.355 ± 0.377
4.541ProLeu: 4.541 ± 0.631
1.345ProMet: 1.345 ± 0.275
2.074ProAsn: 2.074 ± 0.309
4.148ProPro: 4.148 ± 0.585
2.018ProGln: 2.018 ± 0.326
3.083ProArg: 3.083 ± 0.469
3.364ProSer: 3.364 ± 0.49
3.924ProThr: 3.924 ± 0.459
4.653ProVal: 4.653 ± 0.497
1.345ProTrp: 1.345 ± 0.249
1.85ProTyr: 1.85 ± 0.287
0.0ProXaa: 0.0 ± 0.0
Gln
4.373GlnAla: 4.373 ± 0.54
0.448GlnCys: 0.448 ± 0.185
1.57GlnAsp: 1.57 ± 0.279
1.626GlnGlu: 1.626 ± 0.296
1.065GlnPhe: 1.065 ± 0.199
2.523GlnGly: 2.523 ± 0.406
0.953GlnHis: 0.953 ± 0.276
1.682GlnIle: 1.682 ± 0.343
1.345GlnLys: 1.345 ± 0.259
2.747GlnLeu: 2.747 ± 0.373
0.785GlnMet: 0.785 ± 0.21
0.953GlnAsn: 0.953 ± 0.226
3.027GlnPro: 3.027 ± 0.487
1.233GlnGln: 1.233 ± 0.303
2.523GlnArg: 2.523 ± 0.358
1.906GlnSer: 1.906 ± 0.378
1.626GlnThr: 1.626 ± 0.377
2.411GlnVal: 2.411 ± 0.363
0.617GlnTrp: 0.617 ± 0.168
0.841GlnTyr: 0.841 ± 0.233
0.0GlnXaa: 0.0 ± 0.0
Arg
7.176ArgAla: 7.176 ± 0.728
1.177ArgCys: 1.177 ± 0.349
4.373ArgAsp: 4.373 ± 0.569
4.597ArgGlu: 4.597 ± 0.643
2.242ArgPhe: 2.242 ± 0.43
3.812ArgGly: 3.812 ± 0.367
1.514ArgHis: 1.514 ± 0.33
4.429ArgIle: 4.429 ± 0.649
2.803ArgLys: 2.803 ± 0.402
4.709ArgLeu: 4.709 ± 0.627
2.523ArgMet: 2.523 ± 0.41
2.467ArgAsn: 2.467 ± 0.473
3.812ArgPro: 3.812 ± 0.411
1.906ArgGln: 1.906 ± 0.398
5.326ArgArg: 5.326 ± 0.739
3.644ArgSer: 3.644 ± 0.454
3.139ArgThr: 3.139 ± 0.428
5.662ArgVal: 5.662 ± 0.583
1.962ArgTrp: 1.962 ± 0.36
1.906ArgTyr: 1.906 ± 0.31
0.0ArgXaa: 0.0 ± 0.0
Ser
5.214SerAla: 5.214 ± 0.705
0.448SerCys: 0.448 ± 0.191
4.317SerAsp: 4.317 ± 0.518
3.139SerGlu: 3.139 ± 0.459
1.626SerPhe: 1.626 ± 0.397
6.727SerGly: 6.727 ± 0.733
1.402SerHis: 1.402 ± 0.3
3.251SerIle: 3.251 ± 0.416
2.747SerLys: 2.747 ± 0.465
3.756SerLeu: 3.756 ± 0.455
1.458SerMet: 1.458 ± 0.261
2.355SerAsn: 2.355 ± 0.438
2.971SerPro: 2.971 ± 0.431
1.794SerGln: 1.794 ± 0.355
3.364SerArg: 3.364 ± 0.41
3.812SerSer: 3.812 ± 0.602
2.859SerThr: 2.859 ± 0.384
4.036SerVal: 4.036 ± 0.539
1.233SerTrp: 1.233 ± 0.291
1.289SerTyr: 1.289 ± 0.252
0.0SerXaa: 0.0 ± 0.0
Thr
6.559ThrAla: 6.559 ± 0.517
0.897ThrCys: 0.897 ± 0.265
4.261ThrAsp: 4.261 ± 0.579
3.98ThrGlu: 3.98 ± 0.443
2.186ThrPhe: 2.186 ± 0.404
5.886ThrGly: 5.886 ± 0.645
1.738ThrHis: 1.738 ± 0.339
3.588ThrIle: 3.588 ± 0.474
1.682ThrLys: 1.682 ± 0.31
4.373ThrLeu: 4.373 ± 0.582
1.233ThrMet: 1.233 ± 0.264
2.242ThrAsn: 2.242 ± 0.342
4.373ThrPro: 4.373 ± 0.523
1.794ThrGln: 1.794 ± 0.34
3.98ThrArg: 3.98 ± 0.416
3.812ThrSer: 3.812 ± 0.425
4.989ThrThr: 4.989 ± 0.74
6.167ThrVal: 6.167 ± 0.733
1.009ThrTrp: 1.009 ± 0.284
1.906ThrTyr: 1.906 ± 0.336
0.0ThrXaa: 0.0 ± 0.0
Val
6.951ValAla: 6.951 ± 0.599
1.402ValCys: 1.402 ± 0.295
5.494ValAsp: 5.494 ± 0.561
4.877ValGlu: 4.877 ± 0.612
2.355ValPhe: 2.355 ± 0.414
5.494ValGly: 5.494 ± 0.571
1.177ValHis: 1.177 ± 0.269
2.859ValIle: 2.859 ± 0.395
2.467ValLys: 2.467 ± 0.377
4.877ValLeu: 4.877 ± 0.495
1.402ValMet: 1.402 ± 0.277
2.242ValAsn: 2.242 ± 0.387
4.317ValPro: 4.317 ± 0.385
2.971ValGln: 2.971 ± 0.367
4.989ValArg: 4.989 ± 0.586
4.765ValSer: 4.765 ± 0.615
5.326ValThr: 5.326 ± 0.531
6.559ValVal: 6.559 ± 0.689
1.682ValTrp: 1.682 ± 0.337
1.289ValTyr: 1.289 ± 0.249
0.0ValXaa: 0.0 ± 0.0
Trp
1.85TrpAla: 1.85 ± 0.322
0.28TrpCys: 0.28 ± 0.114
1.233TrpAsp: 1.233 ± 0.221
1.177TrpGlu: 1.177 ± 0.299
0.561TrpPhe: 0.561 ± 0.143
1.009TrpGly: 1.009 ± 0.223
0.617TrpHis: 0.617 ± 0.183
1.009TrpIle: 1.009 ± 0.231
0.729TrpLys: 0.729 ± 0.171
1.794TrpLeu: 1.794 ± 0.345
0.897TrpMet: 0.897 ± 0.248
0.729TrpAsn: 0.729 ± 0.242
1.345TrpPro: 1.345 ± 0.295
1.121TrpGln: 1.121 ± 0.23
2.186TrpArg: 2.186 ± 0.422
1.233TrpSer: 1.233 ± 0.257
1.626TrpThr: 1.626 ± 0.313
1.85TrpVal: 1.85 ± 0.424
0.841TrpTrp: 0.841 ± 0.208
0.392TrpTyr: 0.392 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.915TyrAla: 2.915 ± 0.416
0.336TyrCys: 0.336 ± 0.126
1.682TyrAsp: 1.682 ± 0.382
2.074TyrGlu: 2.074 ± 0.354
0.505TyrPhe: 0.505 ± 0.164
1.85TyrGly: 1.85 ± 0.309
0.392TyrHis: 0.392 ± 0.14
1.065TyrIle: 1.065 ± 0.201
0.729TyrLys: 0.729 ± 0.217
2.186TyrLeu: 2.186 ± 0.354
0.224TyrMet: 0.224 ± 0.123
0.729TyrAsn: 0.729 ± 0.197
1.514TyrPro: 1.514 ± 0.293
0.897TyrGln: 0.897 ± 0.218
2.13TyrArg: 2.13 ± 0.361
0.841TyrSer: 0.841 ± 0.209
1.906TyrThr: 1.906 ± 0.396
2.411TyrVal: 2.411 ± 0.286
0.785TyrTrp: 0.785 ± 0.199
0.729TyrTyr: 0.729 ± 0.155
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 100 proteins (17839 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski