Callosobruchus maculatus (Southern cowpea weevil) (Pulse bruchid)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia; Chrysomeloidea; Chrysomelidae; Bruchinae;

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30448 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653CYS7|A0A653CYS7_CALMS Nucleoporin_N domain-containing protein (Fragment) OS=Callosobruchus maculatus OX=64391 GN=CALMAC_LOCUS13003 PE=4 SV=1
MM1 pKa = 7.66LEE3 pKa = 3.58HH4 pKa = 6.78HH5 pKa = 7.48LIYY8 pKa = 10.17LYY10 pKa = 10.97KK11 pKa = 10.53SIHH14 pKa = 4.87QCKK17 pKa = 9.63EE18 pKa = 3.95DD19 pKa = 3.62TDD21 pKa = 4.56SEE23 pKa = 4.22VDD25 pKa = 3.45EE26 pKa = 4.62VQEE29 pKa = 4.37SDD31 pKa = 3.59HH32 pKa = 7.76DD33 pKa = 4.61SDD35 pKa = 5.05TEE37 pKa = 4.35QEE39 pKa = 4.61DD40 pKa = 4.91DD41 pKa = 5.39PLSQDD46 pKa = 3.38NLGGPTFFTIDD57 pKa = 3.05SDD59 pKa = 4.45FYY61 pKa = 11.87ADD63 pKa = 3.34VV64 pKa = 3.6

Molecular weight: 7.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653DVD9|A0A653DVD9_CALMS Isoform of A0A653DXG2 Uncharacterized protein OS=Callosobruchus maculatus OX=64391 GN=CALMAC_LOCUS20788 PE=4 SV=1
VV1 pKa = 6.56GFGVGFFVGRR11 pKa = 11.84TVGLAVGRR19 pKa = 11.84LVGALVGFGVGFFVGGTVGLAVGRR43 pKa = 11.84LVGALVGFGVGFFVGRR59 pKa = 11.84TVGLAVGRR67 pKa = 11.84LVGALVGFGVGFFVGRR83 pKa = 11.84TVGLAVGRR91 pKa = 11.84LVGALVGFLVGFGVGFFVGRR111 pKa = 11.84TVGLAVGRR119 pKa = 11.84LVGALVGSLVGFGVGSFVGFTVGLAVGLLVGFVVGGLVGALVGFSVGVGPSVGLVVGLLVGTVV182 pKa = 3.0

Molecular weight: 17.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20551

9897

30448

11845985

8

21590

389.1

43.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.044 ± 0.018

2.168 ± 0.033

5.469 ± 0.015

6.927 ± 0.031

3.73 ± 0.017

5.483 ± 0.025

2.551 ± 0.012

5.673 ± 0.018

7.148 ± 0.029

8.816 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.31 ± 0.009

4.916 ± 0.016

5.147 ± 0.029

4.311 ± 0.02

5.193 ± 0.014

7.86 ± 0.021

5.861 ± 0.018

6.129 ± 0.013

1.014 ± 0.006

3.249 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski