Trichoderma asperellum CBS 433.97

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma; Trichoderma asperellum

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12547 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T3Z282|A0A2T3Z282_9HYPO Uncharacterized protein OS=Trichoderma asperellum CBS 433.97 OX=1042311 GN=M441DRAFT_60133 PE=4 SV=1
MM1 pKa = 7.61GNYY4 pKa = 9.76SLYY7 pKa = 10.56GFRR10 pKa = 11.84FEE12 pKa = 5.53SEE14 pKa = 4.2SLLLVSDD21 pKa = 3.94QEE23 pKa = 4.32EE24 pKa = 4.44LGNTEE29 pKa = 4.36AEE31 pKa = 4.21YY32 pKa = 10.52EE33 pKa = 4.01QWQNLLEE40 pKa = 4.87LDD42 pKa = 3.76AQASISLQSPNNRR55 pKa = 11.84YY56 pKa = 7.88EE57 pKa = 4.09ANQIPAAEE65 pKa = 4.95HH66 pKa = 6.38ISVQNYY72 pKa = 6.45HH73 pKa = 5.63TQGSDD78 pKa = 2.87NQQTRR83 pKa = 11.84RR84 pKa = 11.84INVAHH89 pKa = 6.75RR90 pKa = 11.84HH91 pKa = 4.17SHH93 pKa = 6.43LPQLIDD99 pKa = 3.08WGSDD103 pKa = 2.9SGSGIEE109 pKa = 5.1DD110 pKa = 2.84IVGIYY115 pKa = 10.83GNDD118 pKa = 3.57PQGLLDD124 pKa = 5.62SPDD127 pKa = 3.42EE128 pKa = 4.83PIWQILYY135 pKa = 10.49SFSYY139 pKa = 10.19QNRR142 pKa = 11.84LDD144 pKa = 4.02PGRR147 pKa = 11.84DD148 pKa = 3.44AYY150 pKa = 10.38EE151 pKa = 4.06HH152 pKa = 5.86TVSAEE157 pKa = 4.0NEE159 pKa = 3.84AGAPHH164 pKa = 6.36ATIKK168 pKa = 11.03NEE170 pKa = 4.25SEE172 pKa = 3.91DD173 pKa = 3.41EE174 pKa = 4.19NYY176 pKa = 10.14PFEE179 pKa = 5.25IMVEE183 pKa = 4.01SGEE186 pKa = 4.21EE187 pKa = 3.9HH188 pKa = 6.69SHH190 pKa = 7.85DD191 pKa = 3.88EE192 pKa = 4.16DD193 pKa = 5.06SIFDD197 pKa = 3.71VVSEE201 pKa = 4.46EE202 pKa = 4.14EE203 pKa = 4.35STDD206 pKa = 3.69DD207 pKa = 3.39EE208 pKa = 5.46CGFEE212 pKa = 5.53SDD214 pKa = 6.1DD215 pKa = 3.84EE216 pKa = 4.82DD217 pKa = 5.71ADD219 pKa = 4.1EE220 pKa = 6.33DD221 pKa = 4.37SDD223 pKa = 5.21SDD225 pKa = 3.61MDD227 pKa = 4.1LSEE230 pKa = 5.92DD231 pKa = 4.31DD232 pKa = 3.71GHH234 pKa = 8.84LEE236 pKa = 3.78LAEE239 pKa = 4.15EE240 pKa = 4.46AGEE243 pKa = 4.24TTYY246 pKa = 11.02MLGGDD251 pKa = 4.07LLVRR255 pKa = 11.84IPTHH259 pKa = 5.84SVFDD263 pKa = 4.06RR264 pKa = 11.84YY265 pKa = 10.91SPEE268 pKa = 3.92EE269 pKa = 3.97YY270 pKa = 10.37LGEE273 pKa = 4.4TILDD277 pKa = 3.82IEE279 pKa = 4.59WPNSGWQNAFNQTEE293 pKa = 4.2QNN295 pKa = 3.41

Molecular weight: 33.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T3ZA42|A0A2T3ZA42_9HYPO Uncharacterized protein OS=Trichoderma asperellum CBS 433.97 OX=1042311 GN=M441DRAFT_402414 PE=4 SV=1
MM1 pKa = 7.94VSINQLLIGIFFFFFKK17 pKa = 10.94KK18 pKa = 10.39NMQSQTRR25 pKa = 11.84NALFRR30 pKa = 11.84PRR32 pKa = 11.84SSNKK36 pKa = 8.32PVRR39 pKa = 11.84PRR41 pKa = 11.84LHH43 pKa = 6.47KK44 pKa = 10.03VWTVSAISLRR54 pKa = 3.81

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12546

1

12547

5543688

49

20866

441.8

48.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.746 ± 0.02

1.333 ± 0.01

5.635 ± 0.015

6.044 ± 0.024

3.804 ± 0.014

6.751 ± 0.024

2.367 ± 0.01

5.224 ± 0.019

4.905 ± 0.017

8.981 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.213 ± 0.009

3.777 ± 0.012

5.849 ± 0.028

4.041 ± 0.017

5.947 ± 0.021

8.355 ± 0.026

5.764 ± 0.016

5.973 ± 0.018

1.488 ± 0.008

2.802 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski