Amino acid dipepetide frequency for Hyphomicrobium sp. (strain MC1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.661AlaAla: 15.661 ± 0.142	1.043AlaCys: 1.043 ± 0.028	6.561AlaAsp: 6.561 ± 0.071	6.97AlaGlu: 6.97 ± 0.086	4.318AlaPhe: 4.318 ± 0.062	9.077AlaGly: 9.077 ± 0.095	2.222AlaHis: 2.222 ± 0.039	6.731AlaIle: 6.731 ± 0.074	5.231AlaLys: 5.231 ± 0.081	12.07AlaLeu: 12.07 ± 0.115	3.24AlaMet: 3.24 ± 0.045	3.352AlaAsn: 3.352 ± 0.048	5.412AlaPro: 5.412 ± 0.086	3.768AlaGln: 3.768 ± 0.064	7.491AlaArg: 7.491 ± 0.081	6.962AlaSer: 6.962 ± 0.075	6.139AlaThr: 6.139 ± 0.072	8.245AlaVal: 8.245 ± 0.081	1.407AlaTrp: 1.407 ± 0.037	2.521AlaTyr: 2.521 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	1.013CysAla: 1.013 ± 0.029	0.147CysCys: 0.147 ± 0.011	0.51CysAsp: 0.51 ± 0.016	0.444CysGlu: 0.444 ± 0.018	0.364CysPhe: 0.364 ± 0.016	0.904CysGly: 0.904 ± 0.025	0.263CysHis: 0.263 ± 0.019	0.443CysIle: 0.443 ± 0.018	0.297CysLys: 0.297 ± 0.016	0.785CysLeu: 0.785 ± 0.024	0.197CysMet: 0.197 ± 0.012	0.242CysAsn: 0.242 ± 0.013	0.501CysPro: 0.501 ± 0.02	0.266CysGln: 0.266 ± 0.013	0.668CysArg: 0.668 ± 0.024	0.584CysSer: 0.584 ± 0.02	0.457CysThr: 0.457 ± 0.018	0.639CysVal: 0.639 ± 0.023	0.113CysTrp: 0.113 ± 0.009	0.233CysTyr: 0.233 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	6.748AspAla: 6.748 ± 0.082	0.526AspCys: 0.526 ± 0.02	3.565AspAsp: 3.565 ± 0.063	3.507AspGlu: 3.507 ± 0.053	2.186AspPhe: 2.186 ± 0.042	4.939AspGly: 4.939 ± 0.064	1.178AspHis: 1.178 ± 0.031	3.271AspIle: 3.271 ± 0.057	2.213AspLys: 2.213 ± 0.045	5.665AspLeu: 5.665 ± 0.076	1.194AspMet: 1.194 ± 0.028	1.456AspAsn: 1.456 ± 0.032	3.069AspPro: 3.069 ± 0.05	1.724AspGln: 1.724 ± 0.038	3.918AspArg: 3.918 ± 0.054	2.226AspSer: 2.226 ± 0.045	2.652AspThr: 2.652 ± 0.046	4.509AspVal: 4.509 ± 0.06	0.928AspTrp: 0.928 ± 0.023	1.457AspTyr: 1.457 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	6.852GluAla: 6.852 ± 0.082	0.381GluCys: 0.381 ± 0.017	2.699GluAsp: 2.699 ± 0.046	2.888GluGlu: 2.888 ± 0.055	1.869GluPhe: 1.869 ± 0.039	3.77GluGly: 3.77 ± 0.053	1.242GluHis: 1.242 ± 0.031	3.659GluIle: 3.659 ± 0.054	2.804GluLys: 2.804 ± 0.048	5.357GluLeu: 5.357 ± 0.061	1.442GluMet: 1.442 ± 0.036	1.693GluAsn: 1.693 ± 0.037	2.685GluPro: 2.685 ± 0.052	2.004GluGln: 2.004 ± 0.044	4.58GluArg: 4.58 ± 0.07	2.492GluSer: 2.492 ± 0.043	3.577GluThr: 3.577 ± 0.067	3.587GluVal: 3.587 ± 0.056	0.676GluTrp: 0.676 ± 0.023	1.031GluTyr: 1.031 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	4.536PheAla: 4.536 ± 0.062	0.425PheCys: 0.425 ± 0.02	2.518PheAsp: 2.518 ± 0.049	2.268PheGlu: 2.268 ± 0.037	1.498PhePhe: 1.498 ± 0.034	3.639PheGly: 3.639 ± 0.056	0.75PheHis: 0.75 ± 0.023	1.903PheIle: 1.903 ± 0.034	1.349PheLys: 1.349 ± 0.034	3.364PheLeu: 3.364 ± 0.055	0.8PheMet: 0.8 ± 0.023	1.182PheAsn: 1.182 ± 0.03	1.658PhePro: 1.658 ± 0.032	1.05PheGln: 1.05 ± 0.025	2.168PheArg: 2.168 ± 0.039	2.469PheSer: 2.469 ± 0.038	1.916PheThr: 1.916 ± 0.045	2.876PheVal: 2.876 ± 0.042	0.589PheTrp: 0.589 ± 0.024	0.922PheTyr: 0.922 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	8.348GlyAla: 8.348 ± 0.076	0.899GlyCys: 0.899 ± 0.029	4.199GlyAsp: 4.199 ± 0.066	4.244GlyGlu: 4.244 ± 0.057	3.462GlyPhe: 3.462 ± 0.056	6.818GlyGly: 6.818 ± 0.102	1.806GlyHis: 1.806 ± 0.031	4.711GlyIle: 4.711 ± 0.069	3.698GlyLys: 3.698 ± 0.06	7.962GlyLeu: 7.962 ± 0.083	2.009GlyMet: 2.009 ± 0.043	2.293GlyAsn: 2.293 ± 0.047	3.361GlyPro: 3.361 ± 0.054	2.627GlyGln: 2.627 ± 0.048	5.474GlyArg: 5.474 ± 0.065	4.867GlySer: 4.867 ± 0.066	4.344GlyThr: 4.344 ± 0.064	5.875GlyVal: 5.875 ± 0.064	1.247GlyTrp: 1.247 ± 0.027	2.323GlyTyr: 2.323 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.143HisAla: 2.143 ± 0.04	0.244HisCys: 0.244 ± 0.015	1.216HisAsp: 1.216 ± 0.033	1.042HisGlu: 1.042 ± 0.026	0.884HisPhe: 0.884 ± 0.028	1.852HisGly: 1.852 ± 0.037	0.591HisHis: 0.591 ± 0.024	1.087HisIle: 1.087 ± 0.029	0.635HisLys: 0.635 ± 0.021	1.947HisLeu: 1.947 ± 0.039	0.534HisMet: 0.534 ± 0.021	0.502HisAsn: 0.502 ± 0.02	1.244HisPro: 1.244 ± 0.033	0.609HisGln: 0.609 ± 0.021	1.367HisArg: 1.367 ± 0.033	1.05HisSer: 1.05 ± 0.027	0.873HisThr: 0.873 ± 0.023	1.538HisVal: 1.538 ± 0.038	0.325HisTrp: 0.325 ± 0.017	0.572HisTyr: 0.572 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.808IleAla: 7.808 ± 0.073	0.594IleCys: 0.594 ± 0.02	3.774IleAsp: 3.774 ± 0.05	3.786IleGlu: 3.786 ± 0.049	1.997IlePhe: 1.997 ± 0.04	4.889IleGly: 4.889 ± 0.071	0.928IleHis: 0.928 ± 0.023	2.696IleIle: 2.696 ± 0.044	2.021IleLys: 2.021 ± 0.037	4.586IleLeu: 4.586 ± 0.057	1.045IleMet: 1.045 ± 0.03	1.636IleAsn: 1.636 ± 0.036	2.553IlePro: 2.553 ± 0.042	1.3IleGln: 1.3 ± 0.026	3.229IleArg: 3.229 ± 0.046	3.548IleSer: 3.548 ± 0.05	2.842IleThr: 2.842 ± 0.044	4.704IleVal: 4.704 ± 0.065	0.642IleTrp: 0.642 ± 0.021	1.263IleTyr: 1.263 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	4.997LysAla: 4.997 ± 0.07	0.271LysCys: 0.271 ± 0.014	2.232LysAsp: 2.232 ± 0.045	2.06LysGlu: 2.06 ± 0.04	1.349LysPhe: 1.349 ± 0.03	2.999LysGly: 2.999 ± 0.05	0.773LysHis: 0.773 ± 0.025	2.43LysIle: 2.43 ± 0.046	2.075LysLys: 2.075 ± 0.047	4.084LysLeu: 4.084 ± 0.067	0.933LysMet: 0.933 ± 0.031	1.239LysAsn: 1.239 ± 0.027	2.64LysPro: 2.64 ± 0.045	1.288LysGln: 1.288 ± 0.029	2.9LysArg: 2.9 ± 0.044	2.802LysSer: 2.802 ± 0.049	2.537LysThr: 2.537 ± 0.043	2.762LysVal: 2.762 ± 0.053	0.476LysTrp: 0.476 ± 0.019	0.864LysTyr: 0.864 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	12.165LeuAla: 12.165 ± 0.101	0.827LeuCys: 0.827 ± 0.022	5.593LeuAsp: 5.593 ± 0.08	5.107LeuGlu: 5.107 ± 0.073	3.49LeuPhe: 3.49 ± 0.059	7.653LeuGly: 7.653 ± 0.076	1.731LeuHis: 1.731 ± 0.035	5.26LeuIle: 5.26 ± 0.067	4.611LeuLys: 4.611 ± 0.057	8.887LeuLeu: 8.887 ± 0.113	2.21LeuMet: 2.21 ± 0.039	2.751LeuAsn: 2.751 ± 0.05	5.189LeuPro: 5.189 ± 0.062	2.837LeuGln: 2.837 ± 0.048	6.147LeuArg: 6.147 ± 0.076	6.851LeuSer: 6.851 ± 0.084	5.555LeuThr: 5.555 ± 0.07	6.822LeuVal: 6.822 ± 0.075	1.115LeuTrp: 1.115 ± 0.034	2.038LeuTyr: 2.038 ± 0.035	0.0LeuXaa: 0.0 ± 0.0
Met	2.822MetAla: 2.822 ± 0.049	0.183MetCys: 0.183 ± 0.012	1.024MetAsp: 1.024 ± 0.028	0.951MetGlu: 0.951 ± 0.024	0.697MetPhe: 0.697 ± 0.022	1.557MetGly: 1.557 ± 0.034	0.496MetHis: 0.496 ± 0.018	1.303MetIle: 1.303 ± 0.028	1.064MetLys: 1.064 ± 0.026	2.48MetLeu: 2.48 ± 0.043	0.649MetMet: 0.649 ± 0.027	0.734MetAsn: 0.734 ± 0.025	1.505MetPro: 1.505 ± 0.037	0.829MetGln: 0.829 ± 0.023	1.836MetArg: 1.836 ± 0.035	1.803MetSer: 1.803 ± 0.032	1.89MetThr: 1.89 ± 0.037	1.407MetVal: 1.407 ± 0.036	0.215MetTrp: 0.215 ± 0.013	0.339MetTyr: 0.339 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.6AsnAla: 3.6 ± 0.052	0.3AsnCys: 0.3 ± 0.015	1.676AsnAsp: 1.676 ± 0.036	1.431AsnGlu: 1.431 ± 0.036	1.132AsnPhe: 1.132 ± 0.026	2.684AsnGly: 2.684 ± 0.052	0.524AsnHis: 0.524 ± 0.019	1.582AsnIle: 1.582 ± 0.033	0.985AsnLys: 0.985 ± 0.028	2.774AsnLeu: 2.774 ± 0.049	0.627AsnMet: 0.627 ± 0.019	0.845AsnAsn: 0.845 ± 0.032	1.846AsnPro: 1.846 ± 0.034	0.808AsnGln: 0.808 ± 0.027	1.757AsnArg: 1.757 ± 0.037	1.632AsnSer: 1.632 ± 0.043	1.431AsnThr: 1.431 ± 0.035	2.298AsnVal: 2.298 ± 0.043	0.467AsnTrp: 0.467 ± 0.019	0.763AsnTyr: 0.763 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	5.618ProAla: 5.618 ± 0.071	0.329ProCys: 0.329 ± 0.017	3.505ProAsp: 3.505 ± 0.049	3.512ProGlu: 3.512 ± 0.054	2.018ProPhe: 2.018 ± 0.041	4.194ProGly: 4.194 ± 0.055	1.109ProHis: 1.109 ± 0.029	2.643ProIle: 2.643 ± 0.043	2.173ProLys: 2.173 ± 0.037	4.544ProLeu: 4.544 ± 0.054	1.238ProMet: 1.238 ± 0.028	1.659ProAsn: 1.659 ± 0.036	2.997ProPro: 2.997 ± 0.076	1.782ProGln: 1.782 ± 0.042	2.87ProArg: 2.87 ± 0.051	3.335ProSer: 3.335 ± 0.054	2.69ProThr: 2.69 ± 0.04	3.766ProVal: 3.766 ± 0.061	0.698ProTrp: 0.698 ± 0.023	1.224ProTyr: 1.224 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	3.556GlnAla: 3.556 ± 0.053	0.225GlnCys: 0.225 ± 0.013	1.447GlnAsp: 1.447 ± 0.034	1.373GlnGlu: 1.373 ± 0.032	1.176GlnPhe: 1.176 ± 0.03	2.09GlnGly: 2.09 ± 0.045	0.686GlnHis: 0.686 ± 0.024	1.919GlnIle: 1.919 ± 0.038	1.429GlnLys: 1.429 ± 0.028	2.927GlnLeu: 2.927 ± 0.051	0.885GlnMet: 0.885 ± 0.024	1.039GlnAsn: 1.039 ± 0.031	1.773GlnPro: 1.773 ± 0.043	1.328GlnGln: 1.328 ± 0.039	2.426GlnArg: 2.426 ± 0.044	2.172GlnSer: 2.172 ± 0.04	1.791GlnThr: 1.791 ± 0.037	2.055GlnVal: 2.055 ± 0.038	0.402GlnTrp: 0.402 ± 0.016	0.666GlnTyr: 0.666 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	6.918ArgAla: 6.918 ± 0.069	0.551ArgCys: 0.551 ± 0.017	3.858ArgAsp: 3.858 ± 0.051	3.851ArgGlu: 3.851 ± 0.062	2.618ArgPhe: 2.618 ± 0.049	4.466ArgGly: 4.466 ± 0.063	1.566ArgHis: 1.566 ± 0.04	3.95ArgIle: 3.95 ± 0.057	2.623ArgLys: 2.623 ± 0.039	7.066ArgLeu: 7.066 ± 0.079	1.684ArgMet: 1.684 ± 0.035	2.065ArgAsn: 2.065 ± 0.042	3.311ArgPro: 3.311 ± 0.05	2.308ArgGln: 2.308 ± 0.041	5.242ArgArg: 5.242 ± 0.084	3.932ArgSer: 3.932 ± 0.052	3.308ArgThr: 3.308 ± 0.046	4.606ArgVal: 4.606 ± 0.056	0.942ArgTrp: 0.942 ± 0.03	1.81ArgTyr: 1.81 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	6.96SerAla: 6.96 ± 0.087	0.523SerCys: 0.523 ± 0.02	3.485SerAsp: 3.485 ± 0.048	3.462SerGlu: 3.462 ± 0.05	2.402SerPhe: 2.402 ± 0.046	5.66SerGly: 5.66 ± 0.069	1.106SerHis: 1.106 ± 0.029	3.221SerIle: 3.221 ± 0.044	2.384SerLys: 2.384 ± 0.036	5.781SerLeu: 5.781 ± 0.077	1.382SerMet: 1.382 ± 0.028	1.804SerAsn: 1.804 ± 0.037	3.23SerPro: 3.23 ± 0.053	1.903SerGln: 1.903 ± 0.046	3.937SerArg: 3.937 ± 0.051	3.908SerSer: 3.908 ± 0.063	3.095SerThr: 3.095 ± 0.051	4.287SerVal: 4.287 ± 0.059	0.824SerTrp: 0.824 ± 0.025	1.431SerTyr: 1.431 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	6.149ThrAla: 6.149 ± 0.072	0.479ThrCys: 0.479 ± 0.021	2.873ThrAsp: 2.873 ± 0.05	2.616ThrGlu: 2.616 ± 0.046	2.123ThrPhe: 2.123 ± 0.044	4.866ThrGly: 4.866 ± 0.064	1.12ThrHis: 1.12 ± 0.029	3.248ThrIle: 3.248 ± 0.05	2.024ThrLys: 2.024 ± 0.037	5.648ThrLeu: 5.648 ± 0.068	1.175ThrMet: 1.175 ± 0.032	1.537ThrAsn: 1.537 ± 0.035	3.293ThrPro: 3.293 ± 0.055	1.561ThrGln: 1.561 ± 0.038	3.123ThrArg: 3.123 ± 0.041	3.34ThrSer: 3.34 ± 0.05	3.182ThrThr: 3.182 ± 0.051	4.184ThrVal: 4.184 ± 0.058	0.719ThrTrp: 0.719 ± 0.023	1.29ThrTyr: 1.29 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	8.863ValAla: 8.863 ± 0.083	0.71ValCys: 0.71 ± 0.022	4.043ValAsp: 4.043 ± 0.055	4.005ValGlu: 4.005 ± 0.059	2.729ValPhe: 2.729 ± 0.047	5.529ValGly: 5.529 ± 0.068	1.346ValHis: 1.346 ± 0.03	4.05ValIle: 4.05 ± 0.057	2.752ValLys: 2.752 ± 0.05	7.032ValLeu: 7.032 ± 0.079	1.727ValMet: 1.727 ± 0.037	2.002ValAsn: 2.002 ± 0.045	3.796ValPro: 3.796 ± 0.053	2.034ValGln: 2.034 ± 0.039	4.713ValArg: 4.713 ± 0.054	4.639ValSer: 4.639 ± 0.054	4.377ValThr: 4.377 ± 0.057	5.706ValVal: 5.706 ± 0.071	0.902ValTrp: 0.902 ± 0.025	1.506ValTyr: 1.506 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.203TrpAla: 1.203 ± 0.031	0.135TrpCys: 0.135 ± 0.011	0.641TrpAsp: 0.641 ± 0.023	0.562TrpGlu: 0.562 ± 0.022	0.534TrpPhe: 0.534 ± 0.019	0.911TrpGly: 0.911 ± 0.028	0.353TrpHis: 0.353 ± 0.016	0.715TrpIle: 0.715 ± 0.025	0.564TrpLys: 0.564 ± 0.023	1.57TrpLeu: 1.57 ± 0.034	0.365TrpMet: 0.365 ± 0.015	0.438TrpAsn: 0.438 ± 0.017	0.676TrpPro: 0.676 ± 0.023	0.551TrpGln: 0.551 ± 0.021	1.082TrpArg: 1.082 ± 0.032	0.864TrpSer: 0.864 ± 0.025	0.8TrpThr: 0.8 ± 0.027	0.794TrpVal: 0.794 ± 0.024	0.225TrpTrp: 0.225 ± 0.013	0.309TrpTyr: 0.309 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.461TyrAla: 2.461 ± 0.042	0.278TyrCys: 0.278 ± 0.014	1.511TyrAsp: 1.511 ± 0.038	1.211TyrGlu: 1.211 ± 0.029	1.033TyrPhe: 1.033 ± 0.031	2.072TyrGly: 2.072 ± 0.051	0.465TyrHis: 0.465 ± 0.02	1.016TyrIle: 1.016 ± 0.026	0.779TyrLys: 0.779 ± 0.025	2.311TyrLeu: 2.311 ± 0.046	0.448TyrMet: 0.448 ± 0.017	0.693TyrAsn: 0.693 ± 0.021	1.164TyrPro: 1.164 ± 0.032	0.789TyrGln: 0.789 ± 0.023	1.747TyrArg: 1.747 ± 0.035	1.31TyrSer: 1.31 ± 0.03	1.094TyrThr: 1.094 ± 0.029	1.801TyrVal: 1.801 ± 0.037	0.378TyrTrp: 0.378 ± 0.016	0.655TyrTyr: 0.655 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4889 proteins (1411884 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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