Bacillus phage Wes44

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gutmannvirinae; Carmenvirus; Bacillus virus Wes44

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346FK31|A0A346FK31_9CAUD Thymidylate synthase OS=Bacillus phage Wes44 OX=2283012 GN=Wes44_27 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.27KK3 pKa = 10.23VLLSSVLGLGVVSLVGCEE21 pKa = 3.68EE22 pKa = 3.91RR23 pKa = 11.84HH24 pKa = 6.39VYY26 pKa = 9.51FQGSLLPVSEE36 pKa = 4.22VEE38 pKa = 4.53EE39 pKa = 3.97RR40 pKa = 11.84LEE42 pKa = 6.25DD43 pKa = 4.52IIEE46 pKa = 4.37DD47 pKa = 3.72QNPDD51 pKa = 2.77FDD53 pKa = 5.02FEE55 pKa = 4.52VSIYY59 pKa = 10.89EE60 pKa = 4.1EE61 pKa = 4.56DD62 pKa = 3.73EE63 pKa = 4.11EE64 pKa = 4.62

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346FK21|A0A346FK21_9CAUD Uncharacterized protein OS=Bacillus phage Wes44 OX=2283012 GN=Wes44_17 PE=4 SV=1
MM1 pKa = 7.11LTHH4 pKa = 6.64GFSRR8 pKa = 11.84VAILNALLSYY18 pKa = 10.47GYY20 pKa = 11.06NSMVAKK26 pKa = 10.4LYY28 pKa = 10.08VEE30 pKa = 4.16RR31 pKa = 11.84FEE33 pKa = 4.93AKK35 pKa = 10.29NKK37 pKa = 10.15IKK39 pKa = 10.53ISS41 pKa = 3.48

Molecular weight: 4.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13159

40

1120

243.7

27.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.405 ± 0.546

0.828 ± 0.147

6.368 ± 0.398

7.242 ± 0.561

3.739 ± 0.148

6.566 ± 0.289

1.695 ± 0.196

6.513 ± 0.227

7.584 ± 0.433

7.599 ± 0.316

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.207 ± 0.257

5.168 ± 0.31

3.594 ± 0.235

3.488 ± 0.192

4.202 ± 0.235

5.548 ± 0.408

6.216 ± 0.423

6.839 ± 0.333

1.444 ± 0.173

3.754 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski