Amino acid dipepetide frequency for Bacillus phage Wes44

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.068AlaAla: 7.068 ± 1.491
0.684AlaCys: 0.684 ± 0.268
3.572AlaAsp: 3.572 ± 0.515
5.32AlaGlu: 5.32 ± 0.855
2.964AlaPhe: 2.964 ± 0.691
5.928AlaGly: 5.928 ± 0.583
0.76AlaHis: 0.76 ± 0.22
7.296AlaIle: 7.296 ± 0.813
6.764AlaLys: 6.764 ± 0.797
6.764AlaLeu: 6.764 ± 0.879
3.42AlaMet: 3.42 ± 0.695
5.244AlaAsn: 5.244 ± 0.505
2.888AlaPro: 2.888 ± 0.584
3.876AlaGln: 3.876 ± 0.688
3.724AlaArg: 3.724 ± 0.515
4.332AlaSer: 4.332 ± 0.585
4.484AlaThr: 4.484 ± 0.647
5.32AlaVal: 5.32 ± 0.637
1.14AlaTrp: 1.14 ± 0.344
2.508AlaTyr: 2.508 ± 0.341
0.0AlaXaa: 0.0 ± 0.0
Cys
0.456CysAla: 0.456 ± 0.173
0.076CysCys: 0.076 ± 0.089
0.684CysAsp: 0.684 ± 0.264
0.836CysGlu: 0.836 ± 0.253
0.304CysPhe: 0.304 ± 0.135
0.608CysGly: 0.608 ± 0.276
0.152CysHis: 0.152 ± 0.088
0.304CysIle: 0.304 ± 0.181
0.608CysLys: 0.608 ± 0.189
0.456CysLeu: 0.456 ± 0.192
0.228CysMet: 0.228 ± 0.143
0.532CysAsn: 0.532 ± 0.174
0.304CysPro: 0.304 ± 0.137
0.228CysGln: 0.228 ± 0.126
0.304CysArg: 0.304 ± 0.166
0.38CysSer: 0.38 ± 0.195
0.988CysThr: 0.988 ± 0.328
0.152CysVal: 0.152 ± 0.132
0.076CysTrp: 0.076 ± 0.077
0.608CysTyr: 0.608 ± 0.22
0.0CysXaa: 0.0 ± 0.0
Asp
4.864AspAla: 4.864 ± 0.564
0.456AspCys: 0.456 ± 0.211
3.876AspAsp: 3.876 ± 0.625
5.624AspGlu: 5.624 ± 0.984
2.964AspPhe: 2.964 ± 0.5
4.712AspGly: 4.712 ± 0.853
1.216AspHis: 1.216 ± 0.29
4.332AspIle: 4.332 ± 0.698
5.092AspLys: 5.092 ± 0.717
4.484AspLeu: 4.484 ± 0.689
2.584AspMet: 2.584 ± 0.428
2.812AspAsn: 2.812 ± 0.491
1.748AspPro: 1.748 ± 0.375
1.824AspGln: 1.824 ± 0.423
2.888AspArg: 2.888 ± 0.441
2.66AspSer: 2.66 ± 0.405
3.8AspThr: 3.8 ± 0.679
3.572AspVal: 3.572 ± 0.509
1.14AspTrp: 1.14 ± 0.279
3.04AspTyr: 3.04 ± 0.558
0.0AspXaa: 0.0 ± 0.0
Glu
6.308GluAla: 6.308 ± 0.713
0.532GluCys: 0.532 ± 0.219
5.776GluAsp: 5.776 ± 0.806
6.916GluGlu: 6.916 ± 1.261
2.964GluPhe: 2.964 ± 0.428
4.104GluGly: 4.104 ± 0.528
1.368GluHis: 1.368 ± 0.365
4.864GluIle: 4.864 ± 0.607
4.408GluLys: 4.408 ± 0.682
6.08GluLeu: 6.08 ± 0.675
2.584GluMet: 2.584 ± 0.445
2.128GluAsn: 2.128 ± 0.335
3.42GluPro: 3.42 ± 0.752
2.888GluGln: 2.888 ± 0.498
3.344GluArg: 3.344 ± 0.606
2.736GluSer: 2.736 ± 0.435
3.116GluThr: 3.116 ± 0.463
5.396GluVal: 5.396 ± 0.655
0.912GluTrp: 0.912 ± 0.274
2.508GluTyr: 2.508 ± 0.513
0.0GluXaa: 0.0 ± 0.0
Phe
2.204PheAla: 2.204 ± 0.508
0.532PheCys: 0.532 ± 0.186
2.812PheAsp: 2.812 ± 0.495
3.268PheGlu: 3.268 ± 0.569
1.368PhePhe: 1.368 ± 0.34
2.128PheGly: 2.128 ± 0.412
0.836PheHis: 0.836 ± 0.273
3.192PheIle: 3.192 ± 0.436
2.736PheLys: 2.736 ± 0.42
2.432PheLeu: 2.432 ± 0.466
0.988PheMet: 0.988 ± 0.289
2.584PheAsn: 2.584 ± 0.434
0.684PhePro: 0.684 ± 0.245
0.76PheGln: 0.76 ± 0.172
0.988PheArg: 0.988 ± 0.221
3.04PheSer: 3.04 ± 0.456
2.736PheThr: 2.736 ± 0.494
2.356PheVal: 2.356 ± 0.374
0.532PheTrp: 0.532 ± 0.174
1.216PheTyr: 1.216 ± 0.256
0.0PheXaa: 0.0 ± 0.0
Gly
5.32GlyAla: 5.32 ± 0.611
0.608GlyCys: 0.608 ± 0.25
3.876GlyAsp: 3.876 ± 0.657
3.952GlyGlu: 3.952 ± 0.604
2.128GlyPhe: 2.128 ± 0.377
4.712GlyGly: 4.712 ± 0.634
1.064GlyHis: 1.064 ± 0.317
4.18GlyIle: 4.18 ± 0.672
7.752GlyLys: 7.752 ± 0.796
5.168GlyLeu: 5.168 ± 0.815
1.748GlyMet: 1.748 ± 0.466
3.04GlyAsn: 3.04 ± 0.433
1.596GlyPro: 1.596 ± 0.571
1.9GlyGln: 1.9 ± 0.355
2.432GlyArg: 2.432 ± 0.505
3.496GlySer: 3.496 ± 0.675
4.104GlyThr: 4.104 ± 0.879
5.32GlyVal: 5.32 ± 0.602
0.76GlyTrp: 0.76 ± 0.25
2.508GlyTyr: 2.508 ± 0.426
0.0GlyXaa: 0.0 ± 0.0
His
1.368HisAla: 1.368 ± 0.313
0.152HisCys: 0.152 ± 0.097
1.216HisAsp: 1.216 ± 0.343
0.912HisGlu: 0.912 ± 0.268
0.684HisPhe: 0.684 ± 0.284
1.14HisGly: 1.14 ± 0.338
0.532HisHis: 0.532 ± 0.208
1.064HisIle: 1.064 ± 0.301
1.064HisLys: 1.064 ± 0.305
1.748HisLeu: 1.748 ± 0.416
0.38HisMet: 0.38 ± 0.126
0.532HisAsn: 0.532 ± 0.163
0.456HisPro: 0.456 ± 0.171
0.38HisGln: 0.38 ± 0.139
0.76HisArg: 0.76 ± 0.204
0.76HisSer: 0.76 ± 0.281
1.368HisThr: 1.368 ± 0.291
0.988HisVal: 0.988 ± 0.304
0.532HisTrp: 0.532 ± 0.221
0.912HisTyr: 0.912 ± 0.27
0.0HisXaa: 0.0 ± 0.0
Ile
5.624IleAla: 5.624 ± 0.562
0.38IleCys: 0.38 ± 0.164
5.32IleAsp: 5.32 ± 0.861
5.092IleGlu: 5.092 ± 0.532
2.204IlePhe: 2.204 ± 0.362
3.952IleGly: 3.952 ± 0.722
1.216IleHis: 1.216 ± 0.307
3.952IleIle: 3.952 ± 0.393
5.092IleLys: 5.092 ± 1.054
4.408IleLeu: 4.408 ± 0.614
2.128IleMet: 2.128 ± 0.333
2.964IleAsn: 2.964 ± 0.458
2.812IlePro: 2.812 ± 0.611
2.128IleGln: 2.128 ± 0.47
3.116IleArg: 3.116 ± 0.592
3.648IleSer: 3.648 ± 0.505
3.876IleThr: 3.876 ± 0.589
4.256IleVal: 4.256 ± 0.575
0.836IleTrp: 0.836 ± 0.424
2.128IleTyr: 2.128 ± 0.356
0.0IleXaa: 0.0 ± 0.0
Lys
7.676LysAla: 7.676 ± 0.926
0.76LysCys: 0.76 ± 0.233
4.332LysAsp: 4.332 ± 0.681
6.916LysGlu: 6.916 ± 1.058
3.116LysPhe: 3.116 ± 0.431
4.864LysGly: 4.864 ± 0.618
1.292LysHis: 1.292 ± 0.357
4.104LysIle: 4.104 ± 0.519
5.244LysLys: 5.244 ± 0.776
5.7LysLeu: 5.7 ± 0.511
2.28LysMet: 2.28 ± 0.497
2.584LysAsn: 2.584 ± 0.407
2.66LysPro: 2.66 ± 0.518
3.192LysGln: 3.192 ± 0.519
3.648LysArg: 3.648 ± 0.556
3.42LysSer: 3.42 ± 0.524
4.18LysThr: 4.18 ± 0.556
5.852LysVal: 5.852 ± 0.789
1.064LysTrp: 1.064 ± 0.295
2.964LysTyr: 2.964 ± 0.395
0.0LysXaa: 0.0 ± 0.0
Leu
6.992LeuAla: 6.992 ± 0.899
0.684LeuCys: 0.684 ± 0.236
5.472LeuAsp: 5.472 ± 0.849
5.472LeuGlu: 5.472 ± 0.69
1.976LeuPhe: 1.976 ± 0.379
4.56LeuGly: 4.56 ± 0.63
1.216LeuHis: 1.216 ± 0.268
4.56LeuIle: 4.56 ± 0.423
5.548LeuLys: 5.548 ± 0.647
4.94LeuLeu: 4.94 ± 0.722
2.356LeuMet: 2.356 ± 0.556
4.408LeuAsn: 4.408 ± 0.569
2.888LeuPro: 2.888 ± 0.626
3.116LeuGln: 3.116 ± 0.608
3.116LeuArg: 3.116 ± 0.502
5.472LeuSer: 5.472 ± 0.678
5.396LeuThr: 5.396 ± 0.596
4.408LeuVal: 4.408 ± 0.661
1.064LeuTrp: 1.064 ± 0.463
2.356LeuTyr: 2.356 ± 0.337
0.0LeuXaa: 0.0 ± 0.0
Met
3.268MetAla: 3.268 ± 0.568
0.228MetCys: 0.228 ± 0.117
2.356MetAsp: 2.356 ± 0.35
2.052MetGlu: 2.052 ± 0.406
1.368MetPhe: 1.368 ± 0.324
2.356MetGly: 2.356 ± 0.538
0.76MetHis: 0.76 ± 0.197
2.204MetIle: 2.204 ± 0.411
1.976MetLys: 1.976 ± 0.397
2.888MetLeu: 2.888 ± 0.49
0.684MetMet: 0.684 ± 0.256
2.052MetAsn: 2.052 ± 0.336
1.292MetPro: 1.292 ± 0.337
0.76MetGln: 0.76 ± 0.282
1.064MetArg: 1.064 ± 0.281
2.28MetSer: 2.28 ± 0.398
1.824MetThr: 1.824 ± 0.39
1.596MetVal: 1.596 ± 0.317
0.0MetTrp: 0.0 ± 0.0
1.064MetTyr: 1.064 ± 0.285
0.0MetXaa: 0.0 ± 0.0
Asn
5.624AsnAla: 5.624 ± 1.188
0.456AsnCys: 0.456 ± 0.243
2.736AsnAsp: 2.736 ± 0.49
3.116AsnGlu: 3.116 ± 0.541
2.052AsnPhe: 2.052 ± 0.332
5.32AsnGly: 5.32 ± 0.748
0.912AsnHis: 0.912 ± 0.327
2.66AsnIle: 2.66 ± 0.375
3.496AsnLys: 3.496 ± 0.429
3.876AsnLeu: 3.876 ± 0.652
1.444AsnMet: 1.444 ± 0.283
2.432AsnAsn: 2.432 ± 0.444
2.052AsnPro: 2.052 ± 0.538
1.292AsnGln: 1.292 ± 0.326
2.204AsnArg: 2.204 ± 0.309
3.496AsnSer: 3.496 ± 0.63
2.736AsnThr: 2.736 ± 0.444
3.116AsnVal: 3.116 ± 0.502
0.684AsnTrp: 0.684 ± 0.301
1.976AsnTyr: 1.976 ± 0.426
0.0AsnXaa: 0.0 ± 0.0
Pro
3.42ProAla: 3.42 ± 0.496
0.228ProCys: 0.228 ± 0.11
2.812ProAsp: 2.812 ± 0.544
2.356ProGlu: 2.356 ± 0.392
1.14ProPhe: 1.14 ± 0.387
1.292ProGly: 1.292 ± 0.353
0.608ProHis: 0.608 ± 0.225
2.28ProIle: 2.28 ± 0.392
2.356ProLys: 2.356 ± 0.516
2.508ProLeu: 2.508 ± 0.402
1.292ProMet: 1.292 ± 0.245
2.432ProAsn: 2.432 ± 0.528
1.292ProPro: 1.292 ± 0.356
0.988ProGln: 0.988 ± 0.308
1.444ProArg: 1.444 ± 0.342
1.824ProSer: 1.824 ± 0.373
2.432ProThr: 2.432 ± 0.645
3.648ProVal: 3.648 ± 0.586
0.532ProTrp: 0.532 ± 0.186
1.064ProTyr: 1.064 ± 0.287
0.0ProXaa: 0.0 ± 0.0
Gln
3.344GlnAla: 3.344 ± 0.366
0.228GlnCys: 0.228 ± 0.144
1.064GlnAsp: 1.064 ± 0.242
2.508GlnGlu: 2.508 ± 0.373
1.368GlnPhe: 1.368 ± 0.298
2.736GlnGly: 2.736 ± 0.39
0.38GlnHis: 0.38 ± 0.175
2.204GlnIle: 2.204 ± 0.407
2.356GlnLys: 2.356 ± 0.489
2.584GlnLeu: 2.584 ± 0.508
1.216GlnMet: 1.216 ± 0.291
1.52GlnAsn: 1.52 ± 0.302
1.216GlnPro: 1.216 ± 0.28
1.976GlnGln: 1.976 ± 0.588
1.292GlnArg: 1.292 ± 0.312
1.672GlnSer: 1.672 ± 0.432
1.52GlnThr: 1.52 ± 0.478
3.572GlnVal: 3.572 ± 0.461
0.836GlnTrp: 0.836 ± 0.279
1.292GlnTyr: 1.292 ± 0.279
0.0GlnXaa: 0.0 ± 0.0
Arg
2.584ArgAla: 2.584 ± 0.525
0.456ArgCys: 0.456 ± 0.183
2.128ArgAsp: 2.128 ± 0.323
2.28ArgGlu: 2.28 ± 0.377
1.368ArgPhe: 1.368 ± 0.31
1.748ArgGly: 1.748 ± 0.413
0.76ArgHis: 0.76 ± 0.227
3.344ArgIle: 3.344 ± 0.512
3.8ArgLys: 3.8 ± 0.559
4.028ArgLeu: 4.028 ± 0.659
1.368ArgMet: 1.368 ± 0.274
1.672ArgAsn: 1.672 ± 0.354
2.052ArgPro: 2.052 ± 0.455
1.52ArgGln: 1.52 ± 0.392
1.9ArgArg: 1.9 ± 0.384
2.736ArgSer: 2.736 ± 0.443
2.888ArgThr: 2.888 ± 0.402
2.812ArgVal: 2.812 ± 0.67
0.76ArgTrp: 0.76 ± 0.225
1.824ArgTyr: 1.824 ± 0.387
0.0ArgXaa: 0.0 ± 0.0
Ser
4.256SerAla: 4.256 ± 0.714
0.38SerCys: 0.38 ± 0.172
3.572SerAsp: 3.572 ± 0.565
3.116SerGlu: 3.116 ± 0.707
1.672SerPhe: 1.672 ± 0.341
3.952SerGly: 3.952 ± 0.604
0.836SerHis: 0.836 ± 0.236
3.268SerIle: 3.268 ± 0.572
4.788SerLys: 4.788 ± 0.539
4.332SerLeu: 4.332 ± 0.591
2.128SerMet: 2.128 ± 0.427
3.496SerAsn: 3.496 ± 0.777
1.596SerPro: 1.596 ± 0.536
1.748SerGln: 1.748 ± 0.368
1.824SerArg: 1.824 ± 0.373
4.104SerSer: 4.104 ± 0.771
4.408SerThr: 4.408 ± 0.614
3.04SerVal: 3.04 ± 0.583
0.608SerTrp: 0.608 ± 0.31
2.356SerTyr: 2.356 ± 0.439
0.0SerXaa: 0.0 ± 0.0
Thr
5.016ThrAla: 5.016 ± 0.553
0.456ThrCys: 0.456 ± 0.208
3.648ThrAsp: 3.648 ± 0.62
4.94ThrGlu: 4.94 ± 0.667
2.812ThrPhe: 2.812 ± 0.556
5.32ThrGly: 5.32 ± 1.132
0.76ThrHis: 0.76 ± 0.246
3.42ThrIle: 3.42 ± 0.632
3.42ThrLys: 3.42 ± 0.445
5.32ThrLeu: 5.32 ± 0.515
1.368ThrMet: 1.368 ± 0.287
3.572ThrAsn: 3.572 ± 0.773
2.964ThrPro: 2.964 ± 0.489
1.9ThrGln: 1.9 ± 0.443
2.052ThrArg: 2.052 ± 0.363
2.584ThrSer: 2.584 ± 0.615
4.028ThrThr: 4.028 ± 0.666
5.32ThrVal: 5.32 ± 0.981
1.14ThrTrp: 1.14 ± 0.37
2.128ThrTyr: 2.128 ± 0.419
0.0ThrXaa: 0.0 ± 0.0
Val
4.408ValAla: 4.408 ± 0.615
0.228ValCys: 0.228 ± 0.125
4.332ValAsp: 4.332 ± 0.583
4.484ValGlu: 4.484 ± 0.726
3.116ValPhe: 3.116 ± 0.406
3.192ValGly: 3.192 ± 0.695
1.14ValHis: 1.14 ± 0.253
4.712ValIle: 4.712 ± 0.609
5.32ValLys: 5.32 ± 0.528
4.788ValLeu: 4.788 ± 0.66
2.432ValMet: 2.432 ± 0.387
3.724ValAsn: 3.724 ± 0.551
2.812ValPro: 2.812 ± 0.5
3.04ValGln: 3.04 ± 0.455
2.888ValArg: 2.888 ± 0.419
3.572ValSer: 3.572 ± 0.725
4.864ValThr: 4.864 ± 0.853
4.56ValVal: 4.56 ± 0.678
1.52ValTrp: 1.52 ± 0.771
3.268ValTyr: 3.268 ± 0.412
0.0ValXaa: 0.0 ± 0.0
Trp
0.912TrpAla: 0.912 ± 0.253
0.228TrpCys: 0.228 ± 0.123
1.672TrpAsp: 1.672 ± 0.382
0.988TrpGlu: 0.988 ± 0.322
0.304TrpPhe: 0.304 ± 0.155
0.76TrpGly: 0.76 ± 0.186
0.228TrpHis: 0.228 ± 0.136
0.684TrpIle: 0.684 ± 0.21
0.836TrpLys: 0.836 ± 0.186
0.912TrpLeu: 0.912 ± 0.315
0.456TrpMet: 0.456 ± 0.184
1.596TrpAsn: 1.596 ± 0.85
0.228TrpPro: 0.228 ± 0.117
0.228TrpGln: 0.228 ± 0.12
0.912TrpArg: 0.912 ± 0.41
0.988TrpSer: 0.988 ± 0.294
1.216TrpThr: 1.216 ± 0.282
0.608TrpVal: 0.608 ± 0.199
0.076TrpTrp: 0.076 ± 0.068
0.608TrpTyr: 0.608 ± 0.225
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.344TyrAla: 3.344 ± 0.577
0.532TyrCys: 0.532 ± 0.225
2.432TyrAsp: 2.432 ± 0.374
2.28TyrGlu: 2.28 ± 0.49
1.52TyrPhe: 1.52 ± 0.336
2.28TyrGly: 2.28 ± 0.357
0.912TyrHis: 0.912 ± 0.264
2.508TyrIle: 2.508 ± 0.583
3.192TyrLys: 3.192 ± 0.506
2.584TyrLeu: 2.584 ± 0.419
0.912TyrMet: 0.912 ± 0.284
2.356TyrAsn: 2.356 ± 0.451
0.988TyrPro: 0.988 ± 0.289
1.14TyrGln: 1.14 ± 0.261
2.128TyrArg: 2.128 ± 0.443
2.28TyrSer: 2.28 ± 0.434
2.204TyrThr: 2.204 ± 0.47
2.508TyrVal: 2.508 ± 0.373
0.228TyrTrp: 0.228 ± 0.115
1.216TyrTyr: 1.216 ± 0.323
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (13159 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski