Amino acid dipepetide frequency for Escherichia phage vB_EcoM_005

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.737AlaAla: 5.737 ± 0.47
0.236AlaCys: 0.236 ± 0.08
3.804AlaAsp: 3.804 ± 0.321
5.458AlaGlu: 5.458 ± 0.42
2.428AlaPhe: 2.428 ± 0.23
4.642AlaGly: 4.642 ± 0.36
1.418AlaHis: 1.418 ± 0.16
4.534AlaIle: 4.534 ± 0.378
4.985AlaLys: 4.985 ± 0.352
6.038AlaLeu: 6.038 ± 0.437
1.676AlaMet: 1.676 ± 0.164
3.159AlaAsn: 3.159 ± 0.228
2.665AlaPro: 2.665 ± 0.265
2.407AlaGln: 2.407 ± 0.225
3.03AlaArg: 3.03 ± 0.239
4.577AlaSer: 4.577 ± 0.376
3.868AlaThr: 3.868 ± 0.441
5.286AlaVal: 5.286 ± 0.37
1.01AlaTrp: 1.01 ± 0.131
2.815AlaTyr: 2.815 ± 0.235
0.0AlaXaa: 0.0 ± 0.0
Cys
0.795CysAla: 0.795 ± 0.122
0.172CysCys: 0.172 ± 0.073
0.774CysAsp: 0.774 ± 0.126
0.688CysGlu: 0.688 ± 0.114
0.387CysPhe: 0.387 ± 0.084
0.795CysGly: 0.795 ± 0.12
0.236CysHis: 0.236 ± 0.071
0.623CysIle: 0.623 ± 0.123
0.688CysLys: 0.688 ± 0.136
0.709CysLeu: 0.709 ± 0.14
0.451CysMet: 0.451 ± 0.09
0.344CysAsn: 0.344 ± 0.081
0.43CysPro: 0.43 ± 0.104
0.258CysGln: 0.258 ± 0.081
0.58CysArg: 0.58 ± 0.11
0.666CysSer: 0.666 ± 0.127
0.451CysThr: 0.451 ± 0.09
0.666CysVal: 0.666 ± 0.133
0.15CysTrp: 0.15 ± 0.051
0.451CysTyr: 0.451 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
4.255AspAla: 4.255 ± 0.329
0.881AspCys: 0.881 ± 0.134
3.889AspAsp: 3.889 ± 0.313
4.599AspGlu: 4.599 ± 0.335
3.417AspPhe: 3.417 ± 0.294
4.663AspGly: 4.663 ± 0.381
0.731AspHis: 0.731 ± 0.111
5.2AspIle: 5.2 ± 0.349
4.663AspLys: 4.663 ± 0.305
5.329AspLeu: 5.329 ± 0.341
1.676AspMet: 1.676 ± 0.183
3.03AspAsn: 3.03 ± 0.278
2.17AspPro: 2.17 ± 0.28
1.633AspGln: 1.633 ± 0.206
2.192AspArg: 2.192 ± 0.223
3.309AspSer: 3.309 ± 0.279
3.008AspThr: 3.008 ± 0.255
4.341AspVal: 4.341 ± 0.325
1.096AspTrp: 1.096 ± 0.18
3.266AspTyr: 3.266 ± 0.298
0.0AspXaa: 0.0 ± 0.0
Glu
5.308GluAla: 5.308 ± 0.428
0.924GluCys: 0.924 ± 0.149
4.534GluAsp: 4.534 ± 0.298
4.942GluGlu: 4.942 ± 0.392
3.782GluPhe: 3.782 ± 0.277
3.782GluGly: 3.782 ± 0.288
1.547GluHis: 1.547 ± 0.211
5.695GluIle: 5.695 ± 0.368
4.534GluLys: 4.534 ± 0.326
7.156GluLeu: 7.156 ± 0.383
2.17GluMet: 2.17 ± 0.221
3.997GluAsn: 3.997 ± 0.296
1.934GluPro: 1.934 ± 0.196
2.622GluGln: 2.622 ± 0.276
2.751GluArg: 2.751 ± 0.263
3.804GluSer: 3.804 ± 0.336
4.147GluThr: 4.147 ± 0.258
5.394GluVal: 5.394 ± 0.363
1.096GluTrp: 1.096 ± 0.156
3.632GluTyr: 3.632 ± 0.284
0.0GluXaa: 0.0 ± 0.0
Phe
2.751PheAla: 2.751 ± 0.232
0.451PheCys: 0.451 ± 0.096
3.331PheAsp: 3.331 ± 0.267
3.331PheGlu: 3.331 ± 0.316
1.633PhePhe: 1.633 ± 0.229
3.073PheGly: 3.073 ± 0.266
0.752PheHis: 0.752 ± 0.132
3.073PheIle: 3.073 ± 0.262
4.427PheLys: 4.427 ± 0.297
2.385PheLeu: 2.385 ± 0.233
1.311PheMet: 1.311 ± 0.158
2.665PheAsn: 2.665 ± 0.257
1.117PhePro: 1.117 ± 0.159
1.483PheGln: 1.483 ± 0.15
2.041PheArg: 2.041 ± 0.201
2.471PheSer: 2.471 ± 0.231
2.385PheThr: 2.385 ± 0.261
2.987PheVal: 2.987 ± 0.275
0.623PheTrp: 0.623 ± 0.094
1.719PheTyr: 1.719 ± 0.207
0.0PheXaa: 0.0 ± 0.0
Gly
3.696GlyAla: 3.696 ± 0.36
0.623GlyCys: 0.623 ± 0.125
3.932GlyAsp: 3.932 ± 0.317
4.319GlyGlu: 4.319 ± 0.338
2.622GlyPhe: 2.622 ± 0.214
4.126GlyGly: 4.126 ± 0.682
1.139GlyHis: 1.139 ± 0.147
4.147GlyIle: 4.147 ± 0.293
4.663GlyLys: 4.663 ± 0.326
5.673GlyLeu: 5.673 ± 0.3
1.805GlyMet: 1.805 ± 0.197
3.395GlyAsn: 3.395 ± 0.395
2.106GlyPro: 2.106 ± 0.218
2.02GlyGln: 2.02 ± 0.226
2.987GlyArg: 2.987 ± 0.256
4.513GlySer: 4.513 ± 0.332
3.868GlyThr: 3.868 ± 0.301
3.503GlyVal: 3.503 ± 0.325
1.096GlyTrp: 1.096 ± 0.163
2.944GlyTyr: 2.944 ± 0.29
0.0GlyXaa: 0.0 ± 0.0
His
0.881HisAla: 0.881 ± 0.143
0.236HisCys: 0.236 ± 0.076
0.924HisAsp: 0.924 ± 0.146
1.074HisGlu: 1.074 ± 0.173
0.988HisPhe: 0.988 ± 0.156
1.031HisGly: 1.031 ± 0.155
0.451HisHis: 0.451 ± 0.12
1.139HisIle: 1.139 ± 0.166
1.397HisLys: 1.397 ± 0.167
1.397HisLeu: 1.397 ± 0.174
0.43HisMet: 0.43 ± 0.095
0.946HisAsn: 0.946 ± 0.151
1.031HisPro: 1.031 ± 0.169
0.43HisGln: 0.43 ± 0.101
0.795HisArg: 0.795 ± 0.142
1.139HisSer: 1.139 ± 0.127
0.988HisThr: 0.988 ± 0.15
1.053HisVal: 1.053 ± 0.154
0.322HisTrp: 0.322 ± 0.079
0.817HisTyr: 0.817 ± 0.128
0.0HisXaa: 0.0 ± 0.0
Ile
4.663IleAla: 4.663 ± 0.315
0.774IleCys: 0.774 ± 0.162
5.222IleAsp: 5.222 ± 0.305
5.071IleGlu: 5.071 ± 0.38
2.364IlePhe: 2.364 ± 0.215
3.288IleGly: 3.288 ± 0.258
1.246IleHis: 1.246 ± 0.19
3.997IleIle: 3.997 ± 0.26
7.048IleLys: 7.048 ± 0.461
4.341IleLeu: 4.341 ± 0.284
1.741IleMet: 1.741 ± 0.202
4.018IleAsn: 4.018 ± 0.334
2.665IlePro: 2.665 ± 0.257
2.364IleGln: 2.364 ± 0.25
3.46IleArg: 3.46 ± 0.262
4.384IleSer: 4.384 ± 0.252
4.341IleThr: 4.341 ± 0.285
4.169IleVal: 4.169 ± 0.318
0.666IleTrp: 0.666 ± 0.115
2.385IleTyr: 2.385 ± 0.233
0.0IleXaa: 0.0 ± 0.0
Lys
6.103LysAla: 6.103 ± 0.496
0.86LysCys: 0.86 ± 0.142
4.663LysAsp: 4.663 ± 0.277
6.404LysGlu: 6.404 ± 0.444
3.438LysPhe: 3.438 ± 0.261
4.685LysGly: 4.685 ± 0.383
1.655LysHis: 1.655 ± 0.187
5.136LysIle: 5.136 ± 0.287
4.663LysLys: 4.663 ± 0.333
6.447LysLeu: 6.447 ± 0.392
2.407LysMet: 2.407 ± 0.228
3.782LysAsn: 3.782 ± 0.253
2.686LysPro: 2.686 ± 0.273
2.665LysGln: 2.665 ± 0.225
3.503LysArg: 3.503 ± 0.297
4.04LysSer: 4.04 ± 0.334
4.427LysThr: 4.427 ± 0.361
5.265LysVal: 5.265 ± 0.343
0.924LysTrp: 0.924 ± 0.123
2.965LysTyr: 2.965 ± 0.213
0.0LysXaa: 0.0 ± 0.0
Leu
5.63LeuAla: 5.63 ± 0.364
0.387LeuCys: 0.387 ± 0.083
4.728LeuAsp: 4.728 ± 0.353
5.802LeuGlu: 5.802 ± 0.387
3.331LeuPhe: 3.331 ± 0.28
3.889LeuGly: 3.889 ± 0.279
1.053LeuHis: 1.053 ± 0.149
4.835LeuIle: 4.835 ± 0.37
6.554LeuLys: 6.554 ± 0.342
5.093LeuLeu: 5.093 ± 0.37
2.213LeuMet: 2.213 ± 0.239
4.749LeuAsn: 4.749 ± 0.297
2.794LeuPro: 2.794 ± 0.232
2.514LeuGln: 2.514 ± 0.223
3.374LeuArg: 3.374 ± 0.275
5.458LeuSer: 5.458 ± 0.362
4.771LeuThr: 4.771 ± 0.358
4.985LeuVal: 4.985 ± 0.33
0.666LeuTrp: 0.666 ± 0.12
3.266LeuTyr: 3.266 ± 0.27
0.0LeuXaa: 0.0 ± 0.0
Met
2.428MetAla: 2.428 ± 0.213
0.387MetCys: 0.387 ± 0.095
1.912MetAsp: 1.912 ± 0.198
1.44MetGlu: 1.44 ± 0.199
1.461MetPhe: 1.461 ± 0.18
1.44MetGly: 1.44 ± 0.187
0.258MetHis: 0.258 ± 0.067
1.891MetIle: 1.891 ± 0.215
2.45MetLys: 2.45 ± 0.225
1.827MetLeu: 1.827 ± 0.183
0.774MetMet: 0.774 ± 0.127
1.461MetAsn: 1.461 ± 0.152
0.688MetPro: 0.688 ± 0.125
0.903MetGln: 0.903 ± 0.148
1.096MetArg: 1.096 ± 0.143
2.127MetSer: 2.127 ± 0.212
1.633MetThr: 1.633 ± 0.156
1.848MetVal: 1.848 ± 0.208
0.387MetTrp: 0.387 ± 0.104
1.074MetTyr: 1.074 ± 0.156
0.0MetXaa: 0.0 ± 0.0
Asn
3.567AsnAla: 3.567 ± 0.259
0.494AsnCys: 0.494 ± 0.108
2.858AsnAsp: 2.858 ± 0.263
3.911AsnGlu: 3.911 ± 0.282
2.772AsnPhe: 2.772 ± 0.223
4.362AsnGly: 4.362 ± 0.355
1.053AsnHis: 1.053 ± 0.165
3.653AsnIle: 3.653 ± 0.307
3.761AsnLys: 3.761 ± 0.276
3.589AsnLeu: 3.589 ± 0.289
1.59AsnMet: 1.59 ± 0.182
2.858AsnAsn: 2.858 ± 0.297
2.686AsnPro: 2.686 ± 0.267
1.762AsnGln: 1.762 ± 0.204
2.149AsnArg: 2.149 ± 0.223
3.438AsnSer: 3.438 ± 0.245
2.622AsnThr: 2.622 ± 0.227
3.223AsnVal: 3.223 ± 0.287
0.645AsnTrp: 0.645 ± 0.123
2.299AsnTyr: 2.299 ± 0.248
0.0AsnXaa: 0.0 ± 0.0
Pro
2.364ProAla: 2.364 ± 0.24
0.258ProCys: 0.258 ± 0.078
2.428ProAsp: 2.428 ± 0.258
3.395ProGlu: 3.395 ± 0.297
1.354ProPhe: 1.354 ± 0.181
2.858ProGly: 2.858 ± 0.242
0.559ProHis: 0.559 ± 0.097
2.235ProIle: 2.235 ± 0.218
2.665ProLys: 2.665 ± 0.241
2.557ProLeu: 2.557 ± 0.244
0.752ProMet: 0.752 ± 0.125
1.827ProAsn: 1.827 ± 0.207
0.86ProPro: 0.86 ± 0.16
1.031ProGln: 1.031 ± 0.11
1.418ProArg: 1.418 ± 0.219
2.127ProSer: 2.127 ± 0.21
2.041ProThr: 2.041 ± 0.207
2.708ProVal: 2.708 ± 0.262
0.58ProTrp: 0.58 ± 0.119
1.311ProTyr: 1.311 ± 0.16
0.0ProXaa: 0.0 ± 0.0
Gln
2.987GlnAla: 2.987 ± 0.275
0.344GlnCys: 0.344 ± 0.092
1.912GlnAsp: 1.912 ± 0.198
2.149GlnGlu: 2.149 ± 0.221
1.526GlnPhe: 1.526 ± 0.173
1.655GlnGly: 1.655 ± 0.173
0.58GlnHis: 0.58 ± 0.116
2.686GlnIle: 2.686 ± 0.236
1.891GlnLys: 1.891 ± 0.23
2.729GlnLeu: 2.729 ± 0.265
0.881GlnMet: 0.881 ± 0.142
1.375GlnAsn: 1.375 ± 0.182
0.967GlnPro: 0.967 ± 0.157
1.031GlnGln: 1.031 ± 0.165
1.87GlnArg: 1.87 ± 0.197
1.762GlnSer: 1.762 ± 0.196
1.977GlnThr: 1.977 ± 0.248
2.213GlnVal: 2.213 ± 0.219
0.817GlnTrp: 0.817 ± 0.128
1.633GlnTyr: 1.633 ± 0.206
0.0GlnXaa: 0.0 ± 0.0
Arg
2.772ArgAla: 2.772 ± 0.311
0.494ArgCys: 0.494 ± 0.11
2.493ArgAsp: 2.493 ± 0.242
2.987ArgGlu: 2.987 ± 0.247
2.041ArgPhe: 2.041 ± 0.227
2.557ArgGly: 2.557 ± 0.224
0.86ArgHis: 0.86 ± 0.144
3.503ArgIle: 3.503 ± 0.287
3.524ArgLys: 3.524 ± 0.303
3.438ArgLeu: 3.438 ± 0.294
1.44ArgMet: 1.44 ± 0.158
2.471ArgAsn: 2.471 ± 0.233
1.117ArgPro: 1.117 ± 0.137
1.698ArgGln: 1.698 ± 0.196
2.256ArgArg: 2.256 ± 0.264
2.643ArgSer: 2.643 ± 0.224
2.342ArgThr: 2.342 ± 0.192
2.879ArgVal: 2.879 ± 0.279
0.709ArgTrp: 0.709 ± 0.122
1.784ArgTyr: 1.784 ± 0.232
0.0ArgXaa: 0.0 ± 0.0
Ser
3.481SerAla: 3.481 ± 0.306
0.774SerCys: 0.774 ± 0.154
4.556SerAsp: 4.556 ± 0.342
4.706SerGlu: 4.706 ± 0.359
2.751SerPhe: 2.751 ± 0.235
4.663SerGly: 4.663 ± 0.298
1.053SerHis: 1.053 ± 0.154
4.169SerIle: 4.169 ± 0.255
5.007SerLys: 5.007 ± 0.37
4.513SerLeu: 4.513 ± 0.305
1.612SerMet: 1.612 ± 0.178
2.879SerAsn: 2.879 ± 0.312
2.192SerPro: 2.192 ± 0.248
2.127SerGln: 2.127 ± 0.238
2.729SerArg: 2.729 ± 0.245
4.276SerSer: 4.276 ± 0.358
3.503SerThr: 3.503 ± 0.342
4.04SerVal: 4.04 ± 0.275
0.881SerTrp: 0.881 ± 0.146
2.879SerTyr: 2.879 ± 0.234
0.0SerXaa: 0.0 ± 0.0
Thr
4.341ThrAla: 4.341 ± 0.335
0.43ThrCys: 0.43 ± 0.095
3.223ThrAsp: 3.223 ± 0.237
4.47ThrGlu: 4.47 ± 0.294
2.579ThrPhe: 2.579 ± 0.222
4.19ThrGly: 4.19 ± 0.303
0.688ThrHis: 0.688 ± 0.119
3.696ThrIle: 3.696 ± 0.239
3.975ThrLys: 3.975 ± 0.277
4.319ThrLeu: 4.319 ± 0.368
1.289ThrMet: 1.289 ± 0.177
2.815ThrAsn: 2.815 ± 0.282
2.536ThrPro: 2.536 ± 0.222
1.59ThrGln: 1.59 ± 0.218
2.965ThrArg: 2.965 ± 0.26
3.718ThrSer: 3.718 ± 0.336
3.137ThrThr: 3.137 ± 0.328
3.911ThrVal: 3.911 ± 0.305
0.602ThrTrp: 0.602 ± 0.107
2.407ThrTyr: 2.407 ± 0.212
0.0ThrXaa: 0.0 ± 0.0
Val
4.276ValAla: 4.276 ± 0.311
0.946ValCys: 0.946 ± 0.158
4.341ValAsp: 4.341 ± 0.338
5.415ValGlu: 5.415 ± 0.349
2.772ValPhe: 2.772 ± 0.292
3.954ValGly: 3.954 ± 0.312
0.967ValHis: 0.967 ± 0.128
4.19ValIle: 4.19 ± 0.275
5.286ValLys: 5.286 ± 0.32
4.706ValLeu: 4.706 ± 0.316
1.719ValMet: 1.719 ± 0.167
3.868ValAsn: 3.868 ± 0.317
2.665ValPro: 2.665 ± 0.271
2.428ValGln: 2.428 ± 0.224
2.643ValArg: 2.643 ± 0.26
4.255ValSer: 4.255 ± 0.284
4.212ValThr: 4.212 ± 0.419
4.556ValVal: 4.556 ± 0.353
0.924ValTrp: 0.924 ± 0.133
3.116ValTyr: 3.116 ± 0.268
0.0ValXaa: 0.0 ± 0.0
Trp
0.838TrpAla: 0.838 ± 0.132
0.15TrpCys: 0.15 ± 0.046
0.838TrpAsp: 0.838 ± 0.154
0.795TrpGlu: 0.795 ± 0.129
0.666TrpPhe: 0.666 ± 0.131
0.516TrpGly: 0.516 ± 0.114
0.172TrpHis: 0.172 ± 0.056
0.881TrpIle: 0.881 ± 0.132
1.461TrpLys: 1.461 ± 0.207
1.074TrpLeu: 1.074 ± 0.153
0.494TrpMet: 0.494 ± 0.096
0.946TrpAsn: 0.946 ± 0.142
0.408TrpPro: 0.408 ± 0.098
0.451TrpGln: 0.451 ± 0.087
0.408TrpArg: 0.408 ± 0.087
0.881TrpSer: 0.881 ± 0.15
0.731TrpThr: 0.731 ± 0.132
1.117TrpVal: 1.117 ± 0.162
0.172TrpTrp: 0.172 ± 0.061
0.86TrpTyr: 0.86 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.901TyrAla: 2.901 ± 0.276
0.494TyrCys: 0.494 ± 0.105
2.987TyrAsp: 2.987 ± 0.262
2.794TyrGlu: 2.794 ± 0.238
1.698TyrPhe: 1.698 ± 0.207
3.008TyrGly: 3.008 ± 0.225
1.053TyrHis: 1.053 ± 0.179
2.922TyrIle: 2.922 ± 0.272
3.073TyrLys: 3.073 ± 0.278
2.772TyrLeu: 2.772 ± 0.24
1.031TyrMet: 1.031 ± 0.151
2.708TyrAsn: 2.708 ± 0.204
1.612TyrPro: 1.612 ± 0.18
1.59TyrGln: 1.59 ± 0.199
1.762TyrArg: 1.762 ± 0.21
3.202TyrSer: 3.202 ± 0.312
2.428TyrThr: 2.428 ± 0.248
3.094TyrVal: 3.094 ± 0.272
0.494TyrTrp: 0.494 ± 0.092
1.655TyrTyr: 1.655 ± 0.228
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 250 proteins (46537 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski