Amino acid dipepetide frequency for Rhodobacteraceae bacterium 9Alg 56

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.802AlaAla: 16.802 ± 0.163	1.131AlaCys: 1.131 ± 0.033	7.024AlaAsp: 7.024 ± 0.086	9.047AlaGlu: 9.047 ± 0.111	4.504AlaPhe: 4.504 ± 0.061	10.638AlaGly: 10.638 ± 0.102	2.25AlaHis: 2.25 ± 0.052	5.908AlaIle: 5.908 ± 0.077	3.532AlaLys: 3.532 ± 0.068	13.477AlaLeu: 13.477 ± 0.151	3.697AlaMet: 3.697 ± 0.061	2.858AlaAsn: 2.858 ± 0.052	5.365AlaPro: 5.365 ± 0.079	3.767AlaGln: 3.767 ± 0.063	8.543AlaArg: 8.543 ± 0.107	5.871AlaSer: 5.871 ± 0.084	6.185AlaThr: 6.185 ± 0.075	8.432AlaVal: 8.432 ± 0.102	1.458AlaTrp: 1.458 ± 0.04	2.529AlaTyr: 2.529 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	1.006CysAla: 1.006 ± 0.028	0.106CysCys: 0.106 ± 0.01	0.555CysAsp: 0.555 ± 0.021	0.512CysGlu: 0.512 ± 0.023	0.346CysPhe: 0.346 ± 0.018	0.97CysGly: 0.97 ± 0.028	0.246CysHis: 0.246 ± 0.015	0.463CysIle: 0.463 ± 0.019	0.201CysLys: 0.201 ± 0.012	0.872CysLeu: 0.872 ± 0.03	0.182CysMet: 0.182 ± 0.013	0.215CysAsn: 0.215 ± 0.013	0.524CysPro: 0.524 ± 0.019	0.218CysGln: 0.218 ± 0.013	0.643CysArg: 0.643 ± 0.024	0.493CysSer: 0.493 ± 0.019	0.479CysThr: 0.479 ± 0.02	0.617CysVal: 0.617 ± 0.026	0.136CysTrp: 0.136 ± 0.011	0.207CysTyr: 0.207 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	6.956AspAla: 6.956 ± 0.071	0.538AspCys: 0.538 ± 0.021	3.287AspAsp: 3.287 ± 0.073	3.505AspGlu: 3.505 ± 0.061	2.385AspPhe: 2.385 ± 0.047	5.61AspGly: 5.61 ± 0.074	1.226AspHis: 1.226 ± 0.033	3.269AspIle: 3.269 ± 0.056	1.496AspLys: 1.496 ± 0.036	6.036AspLeu: 6.036 ± 0.071	1.552AspMet: 1.552 ± 0.036	1.303AspAsn: 1.303 ± 0.037	3.346AspPro: 3.346 ± 0.049	1.576AspGln: 1.576 ± 0.042	3.894AspArg: 3.894 ± 0.06	2.347AspSer: 2.347 ± 0.042	3.019AspThr: 3.019 ± 0.06	4.315AspVal: 4.315 ± 0.066	1.074AspTrp: 1.074 ± 0.031	1.458AspTyr: 1.458 ± 0.04	0.0AspXaa: 0.0 ± 0.0
Glu	8.622GluAla: 8.622 ± 0.096	0.411GluCys: 0.411 ± 0.02	3.631GluAsp: 3.631 ± 0.056	4.187GluGlu: 4.187 ± 0.065	2.026GluPhe: 2.026 ± 0.039	5.252GluGly: 5.252 ± 0.063	1.189GluHis: 1.189 ± 0.033	4.055GluIle: 4.055 ± 0.061	2.269GluLys: 2.269 ± 0.045	5.347GluLeu: 5.347 ± 0.061	2.009GluMet: 2.009 ± 0.037	1.906GluAsn: 1.906 ± 0.043	2.811GluPro: 2.811 ± 0.052	1.941GluGln: 1.941 ± 0.045	4.573GluArg: 4.573 ± 0.069	2.561GluSer: 2.561 ± 0.051	4.243GluThr: 4.243 ± 0.064	4.495GluVal: 4.495 ± 0.073	0.736GluTrp: 0.736 ± 0.026	1.128GluTyr: 1.128 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	4.782PheAla: 4.782 ± 0.061	0.431PheCys: 0.431 ± 0.02	2.721PheAsp: 2.721 ± 0.05	2.433PheGlu: 2.433 ± 0.041	1.578PhePhe: 1.578 ± 0.044	3.966PheGly: 3.966 ± 0.064	0.795PheHis: 0.795 ± 0.025	1.717PheIle: 1.717 ± 0.043	0.86PheLys: 0.86 ± 0.025	3.621PheLeu: 3.621 ± 0.069	0.841PheMet: 0.841 ± 0.027	1.029PheAsn: 1.029 ± 0.032	1.665PhePro: 1.665 ± 0.04	1.069PheGln: 1.069 ± 0.035	2.4PheArg: 2.4 ± 0.042	2.276PheSer: 2.276 ± 0.048	2.19PheThr: 2.19 ± 0.045	2.831PheVal: 2.831 ± 0.053	0.603PheTrp: 0.603 ± 0.026	0.925PheTyr: 0.925 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	9.404GlyAla: 9.404 ± 0.097	0.881GlyCys: 0.881 ± 0.028	4.776GlyAsp: 4.776 ± 0.065	5.111GlyGlu: 5.111 ± 0.067	3.767GlyPhe: 3.767 ± 0.055	7.733GlyGly: 7.733 ± 0.121	2.004GlyHis: 2.004 ± 0.044	4.641GlyIle: 4.641 ± 0.07	2.963GlyLys: 2.963 ± 0.047	9.228GlyLeu: 9.228 ± 0.089	2.57GlyMet: 2.57 ± 0.047	2.354GlyAsn: 2.354 ± 0.056	3.764GlyPro: 3.764 ± 0.05	3.004GlyGln: 3.004 ± 0.054	5.942GlyArg: 5.942 ± 0.081	4.583GlySer: 4.583 ± 0.064	4.98GlyThr: 4.98 ± 0.075	6.262GlyVal: 6.262 ± 0.075	1.53GlyTrp: 1.53 ± 0.036	2.255GlyTyr: 2.255 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.182HisAla: 2.182 ± 0.051	0.251HisCys: 0.251 ± 0.015	1.171HisAsp: 1.171 ± 0.029	1.039HisGlu: 1.039 ± 0.03	0.857HisPhe: 0.857 ± 0.027	1.927HisGly: 1.927 ± 0.048	0.527HisHis: 0.527 ± 0.025	1.019HisIle: 1.019 ± 0.036	0.478HisLys: 0.478 ± 0.022	2.089HisLeu: 2.089 ± 0.048	0.537HisMet: 0.537 ± 0.02	0.499HisAsn: 0.499 ± 0.019	1.336HisPro: 1.336 ± 0.038	0.573HisGln: 0.573 ± 0.022	1.372HisArg: 1.372 ± 0.034	0.949HisSer: 0.949 ± 0.029	0.879HisThr: 0.879 ± 0.031	1.513HisVal: 1.513 ± 0.038	0.337HisTrp: 0.337 ± 0.017	0.526HisTyr: 0.526 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	7.122IleAla: 7.122 ± 0.075	0.618IleCys: 0.618 ± 0.022	3.293IleAsp: 3.293 ± 0.058	3.324IleGlu: 3.324 ± 0.058	1.956IlePhe: 1.956 ± 0.047	5.056IleGly: 5.056 ± 0.073	0.931IleHis: 0.931 ± 0.032	2.332IleIle: 2.332 ± 0.049	1.177IleLys: 1.177 ± 0.032	4.944IleLeu: 4.944 ± 0.069	1.022IleMet: 1.022 ± 0.029	1.403IleAsn: 1.403 ± 0.038	2.333IlePro: 2.333 ± 0.044	1.127IleGln: 1.127 ± 0.029	3.333IleArg: 3.333 ± 0.049	3.247IleSer: 3.247 ± 0.049	3.033IleThr: 3.033 ± 0.052	3.959IleVal: 3.959 ± 0.068	0.695IleTrp: 0.695 ± 0.025	1.183IleTyr: 1.183 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	3.862LysAla: 3.862 ± 0.07	0.194LysCys: 0.194 ± 0.012	1.588LysAsp: 1.588 ± 0.046	1.595LysGlu: 1.595 ± 0.04	0.888LysPhe: 0.888 ± 0.03	2.548LysGly: 2.548 ± 0.056	0.536LysHis: 0.536 ± 0.023	1.606LysIle: 1.606 ± 0.041	1.102LysLys: 1.102 ± 0.038	2.895LysLeu: 2.895 ± 0.054	0.758LysMet: 0.758 ± 0.028	0.728LysAsn: 0.728 ± 0.022	1.759LysPro: 1.759 ± 0.047	0.813LysGln: 0.813 ± 0.032	1.952LysArg: 1.952 ± 0.037	1.776LysSer: 1.776 ± 0.035	1.756LysThr: 1.756 ± 0.043	2.158LysVal: 2.158 ± 0.047	0.383LysTrp: 0.383 ± 0.018	0.607LysTyr: 0.607 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	13.453LeuAla: 13.453 ± 0.145	0.969LeuCys: 0.969 ± 0.028	5.642LeuAsp: 5.642 ± 0.077	5.999LeuGlu: 5.999 ± 0.073	3.686LeuPhe: 3.686 ± 0.069	8.25LeuGly: 8.25 ± 0.089	1.884LeuHis: 1.884 ± 0.036	5.029LeuIle: 5.029 ± 0.074	3.164LeuLys: 3.164 ± 0.057	9.33LeuLeu: 9.33 ± 0.117	2.596LeuMet: 2.596 ± 0.045	2.681LeuAsn: 2.681 ± 0.05	5.657LeuPro: 5.657 ± 0.079	2.753LeuGln: 2.753 ± 0.051	7.089LeuArg: 7.089 ± 0.085	6.488LeuSer: 6.488 ± 0.077	5.864LeuThr: 5.864 ± 0.08	6.869LeuVal: 6.869 ± 0.086	1.255LeuTrp: 1.255 ± 0.034	1.951LeuTyr: 1.951 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	3.381MetAla: 3.381 ± 0.047	0.174MetCys: 0.174 ± 0.013	1.352MetAsp: 1.352 ± 0.032	1.501MetGlu: 1.501 ± 0.036	0.827MetPhe: 0.827 ± 0.028	2.098MetGly: 2.098 ± 0.045	0.442MetHis: 0.442 ± 0.02	1.485MetIle: 1.485 ± 0.033	0.984MetLys: 0.984 ± 0.03	2.731MetLeu: 2.731 ± 0.044	0.738MetMet: 0.738 ± 0.026	0.84MetAsn: 0.84 ± 0.026	1.56MetPro: 1.56 ± 0.032	0.947MetGln: 0.947 ± 0.032	1.828MetArg: 1.828 ± 0.044	1.659MetSer: 1.659 ± 0.036	1.786MetThr: 1.786 ± 0.042	1.888MetVal: 1.888 ± 0.039	0.231MetTrp: 0.231 ± 0.014	0.344MetTyr: 0.344 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.18AsnAla: 3.18 ± 0.057	0.257AsnCys: 0.257 ± 0.014	1.43AsnAsp: 1.43 ± 0.047	1.232AsnGlu: 1.232 ± 0.034	1.057AsnPhe: 1.057 ± 0.033	2.475AsnGly: 2.475 ± 0.06	0.519AsnHis: 0.519 ± 0.02	1.487AsnIle: 1.487 ± 0.033	0.625AsnLys: 0.625 ± 0.023	2.551AsnLeu: 2.551 ± 0.049	0.66AsnMet: 0.66 ± 0.022	0.728AsnAsn: 0.728 ± 0.028	1.799AsnPro: 1.799 ± 0.031	0.711AsnGln: 0.711 ± 0.025	1.719AsnArg: 1.719 ± 0.042	1.388AsnSer: 1.388 ± 0.037	1.354AsnThr: 1.354 ± 0.035	1.89AsnVal: 1.89 ± 0.041	0.473AsnTrp: 0.473 ± 0.021	0.698AsnTyr: 0.698 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	5.74ProAla: 5.74 ± 0.081	0.335ProCys: 0.335 ± 0.017	3.761ProAsp: 3.761 ± 0.058	4.546ProGlu: 4.546 ± 0.067	2.023ProPhe: 2.023 ± 0.045	4.542ProGly: 4.542 ± 0.057	1.065ProHis: 1.065 ± 0.028	2.117ProIle: 2.117 ± 0.042	1.589ProLys: 1.589 ± 0.041	4.723ProLeu: 4.723 ± 0.066	1.281ProMet: 1.281 ± 0.035	1.299ProAsn: 1.299 ± 0.035	2.483ProPro: 2.483 ± 0.058	1.529ProGln: 1.529 ± 0.039	2.771ProArg: 2.771 ± 0.053	2.529ProSer: 2.529 ± 0.052	2.302ProThr: 2.302 ± 0.046	4.256ProVal: 4.256 ± 0.054	0.665ProTrp: 0.665 ± 0.026	1.129ProTyr: 1.129 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.678GlnAla: 3.678 ± 0.065	0.209GlnCys: 0.209 ± 0.014	1.557GlnAsp: 1.557 ± 0.037	1.769GlnGlu: 1.769 ± 0.046	1.038GlnPhe: 1.038 ± 0.027	2.413GlnGly: 2.413 ± 0.046	0.553GlnHis: 0.553 ± 0.022	1.823GlnIle: 1.823 ± 0.041	1.036GlnLys: 1.036 ± 0.033	2.529GlnLeu: 2.529 ± 0.052	0.977GlnMet: 0.977 ± 0.029	0.856GlnAsn: 0.856 ± 0.027	1.606GlnPro: 1.606 ± 0.039	1.035GlnGln: 1.035 ± 0.036	2.186GlnArg: 2.186 ± 0.053	1.743GlnSer: 1.743 ± 0.038	1.687GlnThr: 1.687 ± 0.04	2.147GlnVal: 2.147 ± 0.044	0.344GlnTrp: 0.344 ± 0.017	0.547GlnTyr: 0.547 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	7.675ArgAla: 7.675 ± 0.103	0.514ArgCys: 0.514 ± 0.023	3.971ArgAsp: 3.971 ± 0.064	4.208ArgGlu: 4.208 ± 0.062	2.785ArgPhe: 2.785 ± 0.047	4.68ArgGly: 4.68 ± 0.074	1.537ArgHis: 1.537 ± 0.038	3.971ArgIle: 3.971 ± 0.061	2.242ArgLys: 2.242 ± 0.04	7.385ArgLeu: 7.385 ± 0.089	2.014ArgMet: 2.014 ± 0.036	1.953ArgAsn: 1.953 ± 0.04	3.35ArgPro: 3.35 ± 0.061	2.409ArgGln: 2.409 ± 0.054	5.228ArgArg: 5.228 ± 0.084	3.501ArgSer: 3.501 ± 0.053	3.364ArgThr: 3.364 ± 0.054	4.526ArgVal: 4.526 ± 0.066	0.913ArgTrp: 0.913 ± 0.031	1.563ArgTyr: 1.563 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	6.107SerAla: 6.107 ± 0.081	0.412SerCys: 0.412 ± 0.018	3.311SerAsp: 3.311 ± 0.057	3.265SerGlu: 3.265 ± 0.053	2.319SerPhe: 2.319 ± 0.05	5.723SerGly: 5.723 ± 0.078	1.089SerHis: 1.089 ± 0.03	2.647SerIle: 2.647 ± 0.049	1.378SerLys: 1.378 ± 0.035	5.389SerLeu: 5.389 ± 0.067	1.269SerMet: 1.269 ± 0.032	1.383SerAsn: 1.383 ± 0.04	2.673SerPro: 2.673 ± 0.058	1.507SerGln: 1.507 ± 0.04	3.612SerArg: 3.612 ± 0.056	2.761SerSer: 2.761 ± 0.05	2.559SerThr: 2.559 ± 0.045	4.048SerVal: 4.048 ± 0.066	0.655SerTrp: 0.655 ± 0.024	1.289SerTyr: 1.289 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	6.433ThrAla: 6.433 ± 0.081	0.529ThrCys: 0.529 ± 0.021	3.0ThrAsp: 3.0 ± 0.056	3.254ThrGlu: 3.254 ± 0.06	2.108ThrPhe: 2.108 ± 0.047	5.594ThrGly: 5.594 ± 0.065	1.074ThrHis: 1.074 ± 0.031	2.814ThrIle: 2.814 ± 0.048	1.396ThrLys: 1.396 ± 0.035	6.125ThrLeu: 6.125 ± 0.071	1.256ThrMet: 1.256 ± 0.031	1.296ThrAsn: 1.296 ± 0.037	3.266ThrPro: 3.266 ± 0.054	1.391ThrGln: 1.391 ± 0.035	3.509ThrArg: 3.509 ± 0.05	2.822ThrSer: 2.822 ± 0.049	2.799ThrThr: 2.799 ± 0.053	4.39ThrVal: 4.39 ± 0.053	0.637ThrTrp: 0.637 ± 0.023	1.29ThrTyr: 1.29 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	8.881ValAla: 8.881 ± 0.087	0.675ValCys: 0.675 ± 0.026	3.957ValAsp: 3.957 ± 0.064	4.835ValGlu: 4.835 ± 0.059	3.053ValPhe: 3.053 ± 0.059	5.27ValGly: 5.27 ± 0.073	1.347ValHis: 1.347 ± 0.034	4.075ValIle: 4.075 ± 0.063	2.02ValLys: 2.02 ± 0.05	7.425ValLeu: 7.425 ± 0.095	1.98ValMet: 1.98 ± 0.039	1.893ValAsn: 1.893 ± 0.045	3.659ValPro: 3.659 ± 0.056	2.015ValGln: 2.015 ± 0.038	4.432ValArg: 4.432 ± 0.066	4.389ValSer: 4.389 ± 0.054	4.656ValThr: 4.656 ± 0.069	5.468ValVal: 5.468 ± 0.084	0.928ValTrp: 0.928 ± 0.028	1.468ValTyr: 1.468 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.3TrpAla: 1.3 ± 0.036	0.143TrpCys: 0.143 ± 0.011	0.725TrpAsp: 0.725 ± 0.027	0.717TrpGlu: 0.717 ± 0.026	0.564TrpPhe: 0.564 ± 0.022	0.961TrpGly: 0.961 ± 0.03	0.339TrpHis: 0.339 ± 0.019	0.687TrpIle: 0.687 ± 0.025	0.456TrpLys: 0.456 ± 0.021	1.586TrpLeu: 1.586 ± 0.039	0.399TrpMet: 0.399 ± 0.018	0.418TrpAsn: 0.418 ± 0.019	0.67TrpPro: 0.67 ± 0.025	0.674TrpGln: 0.674 ± 0.024	1.126TrpArg: 1.126 ± 0.033	0.804TrpSer: 0.804 ± 0.03	0.7TrpThr: 0.7 ± 0.029	0.845TrpVal: 0.845 ± 0.028	0.235TrpTrp: 0.235 ± 0.016	0.3TrpTyr: 0.3 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.453TyrAla: 2.453 ± 0.049	0.212TyrCys: 0.212 ± 0.012	1.442TyrAsp: 1.442 ± 0.039	1.313TyrGlu: 1.313 ± 0.036	0.966TyrPhe: 0.966 ± 0.029	2.047TyrGly: 2.047 ± 0.046	0.509TyrHis: 0.509 ± 0.024	0.945TyrIle: 0.945 ± 0.029	0.538TyrLys: 0.538 ± 0.024	2.327TyrLeu: 2.327 ± 0.048	0.456TyrMet: 0.456 ± 0.018	0.591TyrAsn: 0.591 ± 0.023	1.061TyrPro: 1.061 ± 0.031	0.673TyrGln: 0.673 ± 0.023	1.635TyrArg: 1.635 ± 0.034	1.208TyrSer: 1.208 ± 0.033	1.148TyrThr: 1.148 ± 0.032	1.514TyrVal: 1.514 ± 0.035	0.363TyrTrp: 0.363 ± 0.02	0.565TyrTyr: 0.565 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3843 proteins (1205218 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---