Amino acid dipepetide frequency for Vibrio phage ICP3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.21AlaAla: 9.21 ± 1.08
0.586AlaCys: 0.586 ± 0.253
5.023AlaAsp: 5.023 ± 0.866
5.861AlaGlu: 5.861 ± 0.894
2.679AlaPhe: 2.679 ± 0.429
5.693AlaGly: 5.693 ± 0.812
1.088AlaHis: 1.088 ± 0.284
5.526AlaIle: 5.526 ± 0.791
6.782AlaLys: 6.782 ± 0.817
7.117AlaLeu: 7.117 ± 0.992
3.098AlaMet: 3.098 ± 0.657
4.27AlaAsn: 4.27 ± 0.544
2.344AlaPro: 2.344 ± 0.384
3.851AlaGln: 3.851 ± 0.778
3.684AlaArg: 3.684 ± 0.461
5.107AlaSer: 5.107 ± 0.763
4.102AlaThr: 4.102 ± 0.724
5.442AlaVal: 5.442 ± 0.684
1.256AlaTrp: 1.256 ± 0.519
3.768AlaTyr: 3.768 ± 0.768
0.0AlaXaa: 0.0 ± 0.0
Cys
1.005CysAla: 1.005 ± 0.295
0.251CysCys: 0.251 ± 0.161
0.502CysAsp: 0.502 ± 0.231
0.837CysGlu: 0.837 ± 0.336
0.837CysPhe: 0.837 ± 0.314
0.67CysGly: 0.67 ± 0.266
0.586CysHis: 0.586 ± 0.189
0.67CysIle: 0.67 ± 0.324
0.419CysLys: 0.419 ± 0.227
0.837CysLeu: 0.837 ± 0.301
0.335CysMet: 0.335 ± 0.232
0.251CysAsn: 0.251 ± 0.191
0.837CysPro: 0.837 ± 0.248
0.335CysGln: 0.335 ± 0.203
0.837CysArg: 0.837 ± 0.331
0.502CysSer: 0.502 ± 0.287
0.251CysThr: 0.251 ± 0.169
0.586CysVal: 0.586 ± 0.253
0.167CysTrp: 0.167 ± 0.153
0.167CysTyr: 0.167 ± 0.185
0.0CysXaa: 0.0 ± 0.0
Asp
5.023AspAla: 5.023 ± 0.888
1.005AspCys: 1.005 ± 0.331
3.684AspAsp: 3.684 ± 0.823
3.684AspGlu: 3.684 ± 0.557
2.763AspPhe: 2.763 ± 0.461
4.521AspGly: 4.521 ± 0.622
0.754AspHis: 0.754 ± 0.228
3.516AspIle: 3.516 ± 0.557
3.935AspLys: 3.935 ± 0.763
3.935AspLeu: 3.935 ± 0.538
1.674AspMet: 1.674 ± 0.331
2.595AspAsn: 2.595 ± 0.518
2.428AspPro: 2.428 ± 0.571
1.423AspGln: 1.423 ± 0.394
2.093AspArg: 2.093 ± 0.421
3.014AspSer: 3.014 ± 0.614
3.935AspThr: 3.935 ± 0.636
4.521AspVal: 4.521 ± 0.617
1.005AspTrp: 1.005 ± 0.312
2.512AspTyr: 2.512 ± 0.616
0.0AspXaa: 0.0 ± 0.0
Glu
8.372GluAla: 8.372 ± 0.892
0.67GluCys: 0.67 ± 0.234
5.526GluAsp: 5.526 ± 0.725
6.279GluGlu: 6.279 ± 0.802
2.261GluPhe: 2.261 ± 0.366
5.275GluGly: 5.275 ± 0.843
1.591GluHis: 1.591 ± 0.393
4.102GluIle: 4.102 ± 0.57
3.516GluLys: 3.516 ± 0.404
6.196GluLeu: 6.196 ± 0.763
2.847GluMet: 2.847 ± 0.551
2.512GluAsn: 2.512 ± 0.453
1.591GluPro: 1.591 ± 0.38
3.684GluGln: 3.684 ± 0.593
3.851GluArg: 3.851 ± 0.555
4.605GluSer: 4.605 ± 0.78
3.098GluThr: 3.098 ± 0.538
4.605GluVal: 4.605 ± 0.678
1.34GluTrp: 1.34 ± 0.303
2.763GluTyr: 2.763 ± 0.367
0.0GluXaa: 0.0 ± 0.0
Phe
2.847PheAla: 2.847 ± 0.607
0.754PheCys: 0.754 ± 0.249
2.679PheAsp: 2.679 ± 0.545
3.098PheGlu: 3.098 ± 0.469
1.34PhePhe: 1.34 ± 0.433
3.098PheGly: 3.098 ± 0.531
0.67PheHis: 0.67 ± 0.288
2.512PheIle: 2.512 ± 0.494
3.014PheLys: 3.014 ± 0.621
3.516PheLeu: 3.516 ± 0.632
1.674PheMet: 1.674 ± 0.37
2.763PheAsn: 2.763 ± 0.662
0.921PhePro: 0.921 ± 0.265
1.423PheGln: 1.423 ± 0.335
1.674PheArg: 1.674 ± 0.335
2.847PheSer: 2.847 ± 0.467
2.595PheThr: 2.595 ± 0.503
2.428PheVal: 2.428 ± 0.609
0.419PheTrp: 0.419 ± 0.211
1.507PheTyr: 1.507 ± 0.346
0.0PheXaa: 0.0 ± 0.0
Gly
6.196GlyAla: 6.196 ± 0.863
0.419GlyCys: 0.419 ± 0.242
3.851GlyAsp: 3.851 ± 0.614
5.191GlyGlu: 5.191 ± 0.69
2.595GlyPhe: 2.595 ± 0.39
5.358GlyGly: 5.358 ± 0.729
1.423GlyHis: 1.423 ± 0.358
3.349GlyIle: 3.349 ± 0.546
5.191GlyLys: 5.191 ± 0.965
6.53GlyLeu: 6.53 ± 0.998
2.428GlyMet: 2.428 ± 0.544
3.014GlyAsn: 3.014 ± 0.5
0.084GlyPro: 0.084 ± 0.088
2.847GlyGln: 2.847 ± 0.515
3.6GlyArg: 3.6 ± 0.359
3.768GlySer: 3.768 ± 0.628
4.102GlyThr: 4.102 ± 0.562
4.354GlyVal: 4.354 ± 0.708
1.005GlyTrp: 1.005 ± 0.312
3.349GlyTyr: 3.349 ± 0.389
0.0GlyXaa: 0.0 ± 0.0
His
1.172HisAla: 1.172 ± 0.306
0.251HisCys: 0.251 ± 0.151
1.088HisAsp: 1.088 ± 0.327
1.507HisGlu: 1.507 ± 0.35
1.256HisPhe: 1.256 ± 0.28
0.921HisGly: 0.921 ± 0.261
0.251HisHis: 0.251 ± 0.141
1.172HisIle: 1.172 ± 0.275
0.837HisLys: 0.837 ± 0.265
1.423HisLeu: 1.423 ± 0.419
0.419HisMet: 0.419 ± 0.215
0.586HisAsn: 0.586 ± 0.264
0.586HisPro: 0.586 ± 0.218
0.335HisGln: 0.335 ± 0.175
1.088HisArg: 1.088 ± 0.357
1.256HisSer: 1.256 ± 0.388
0.754HisThr: 0.754 ± 0.298
1.591HisVal: 1.591 ± 0.333
0.586HisTrp: 0.586 ± 0.258
0.419HisTyr: 0.419 ± 0.23
0.0HisXaa: 0.0 ± 0.0
Ile
4.102IleAla: 4.102 ± 0.535
0.67IleCys: 0.67 ± 0.337
2.847IleAsp: 2.847 ± 0.598
4.186IleGlu: 4.186 ± 0.634
1.34IlePhe: 1.34 ± 0.401
3.516IleGly: 3.516 ± 0.467
0.837IleHis: 0.837 ± 0.307
2.93IleIle: 2.93 ± 0.657
4.605IleLys: 4.605 ± 0.62
4.605IleLeu: 4.605 ± 0.57
1.34IleMet: 1.34 ± 0.318
3.098IleAsn: 3.098 ± 0.574
2.512IlePro: 2.512 ± 0.444
2.428IleGln: 2.428 ± 0.608
3.098IleArg: 3.098 ± 0.593
3.349IleSer: 3.349 ± 0.585
3.349IleThr: 3.349 ± 0.602
3.768IleVal: 3.768 ± 0.509
0.586IleTrp: 0.586 ± 0.247
1.423IleTyr: 1.423 ± 0.384
0.0IleXaa: 0.0 ± 0.0
Lys
6.279LysAla: 6.279 ± 0.815
0.837LysCys: 0.837 ± 0.326
4.186LysAsp: 4.186 ± 0.655
4.772LysGlu: 4.772 ± 0.689
3.014LysPhe: 3.014 ± 0.501
4.437LysGly: 4.437 ± 0.665
1.34LysHis: 1.34 ± 0.483
2.679LysIle: 2.679 ± 0.397
4.689LysLys: 4.689 ± 0.784
5.526LysLeu: 5.526 ± 0.631
2.428LysMet: 2.428 ± 0.45
2.679LysAsn: 2.679 ± 0.58
3.014LysPro: 3.014 ± 0.898
3.349LysGln: 3.349 ± 0.64
3.684LysArg: 3.684 ± 0.578
3.684LysSer: 3.684 ± 0.659
4.019LysThr: 4.019 ± 0.554
4.437LysVal: 4.437 ± 0.772
1.005LysTrp: 1.005 ± 0.386
2.679LysTyr: 2.679 ± 0.596
0.0LysXaa: 0.0 ± 0.0
Leu
6.698LeuAla: 6.698 ± 0.832
1.005LeuCys: 1.005 ± 0.42
3.768LeuAsp: 3.768 ± 0.441
6.698LeuGlu: 6.698 ± 0.833
2.763LeuPhe: 2.763 ± 0.529
5.023LeuGly: 5.023 ± 0.867
1.507LeuHis: 1.507 ± 0.356
5.191LeuIle: 5.191 ± 0.606
7.703LeuLys: 7.703 ± 0.812
5.61LeuLeu: 5.61 ± 0.932
2.428LeuMet: 2.428 ± 0.387
4.354LeuAsn: 4.354 ± 0.524
2.847LeuPro: 2.847 ± 0.441
3.768LeuGln: 3.768 ± 0.557
4.856LeuArg: 4.856 ± 0.675
4.102LeuSer: 4.102 ± 0.585
5.107LeuThr: 5.107 ± 0.675
4.689LeuVal: 4.689 ± 0.541
1.256LeuTrp: 1.256 ± 0.322
2.847LeuTyr: 2.847 ± 0.456
0.0LeuXaa: 0.0 ± 0.0
Met
3.182MetAla: 3.182 ± 0.524
0.419MetCys: 0.419 ± 0.233
1.256MetAsp: 1.256 ± 0.329
2.428MetGlu: 2.428 ± 0.41
1.172MetPhe: 1.172 ± 0.392
1.758MetGly: 1.758 ± 0.517
0.335MetHis: 0.335 ± 0.167
1.172MetIle: 1.172 ± 0.276
1.926MetLys: 1.926 ± 0.411
2.093MetLeu: 2.093 ± 0.384
0.419MetMet: 0.419 ± 0.169
1.758MetAsn: 1.758 ± 0.45
1.591MetPro: 1.591 ± 0.429
1.674MetGln: 1.674 ± 0.424
1.423MetArg: 1.423 ± 0.436
1.758MetSer: 1.758 ± 0.335
2.763MetThr: 2.763 ± 0.666
2.344MetVal: 2.344 ± 0.453
0.167MetTrp: 0.167 ± 0.12
0.502MetTyr: 0.502 ± 0.195
0.0MetXaa: 0.0 ± 0.0
Asn
3.349AsnAla: 3.349 ± 0.636
0.502AsnCys: 0.502 ± 0.203
2.595AsnAsp: 2.595 ± 0.46
2.512AsnGlu: 2.512 ± 0.505
2.177AsnPhe: 2.177 ± 0.421
3.768AsnGly: 3.768 ± 0.597
0.921AsnHis: 0.921 ± 0.278
2.261AsnIle: 2.261 ± 0.543
2.595AsnLys: 2.595 ± 0.567
4.856AsnLeu: 4.856 ± 0.684
1.842AsnMet: 1.842 ± 0.514
2.093AsnAsn: 2.093 ± 0.396
2.93AsnPro: 2.93 ± 0.474
1.591AsnGln: 1.591 ± 0.446
2.261AsnArg: 2.261 ± 0.503
2.679AsnSer: 2.679 ± 0.6
2.512AsnThr: 2.512 ± 0.585
3.516AsnVal: 3.516 ± 0.452
0.586AsnTrp: 0.586 ± 0.266
1.674AsnTyr: 1.674 ± 0.429
0.0AsnXaa: 0.0 ± 0.0
Pro
2.763ProAla: 2.763 ± 0.585
0.335ProCys: 0.335 ± 0.172
2.595ProAsp: 2.595 ± 0.474
4.437ProGlu: 4.437 ± 0.453
1.591ProPhe: 1.591 ± 0.392
0.0ProGly: 0.0 ± 0.0
0.837ProHis: 0.837 ± 0.243
1.34ProIle: 1.34 ± 0.388
1.674ProLys: 1.674 ± 0.41
2.428ProLeu: 2.428 ± 0.46
0.837ProMet: 0.837 ± 0.302
2.595ProAsn: 2.595 ± 0.524
0.67ProPro: 0.67 ± 0.222
1.507ProGln: 1.507 ± 0.5
1.172ProArg: 1.172 ± 0.286
3.014ProSer: 3.014 ± 0.422
1.842ProThr: 1.842 ± 0.425
3.935ProVal: 3.935 ± 0.482
0.67ProTrp: 0.67 ± 0.268
1.256ProTyr: 1.256 ± 0.401
0.0ProXaa: 0.0 ± 0.0
Gln
5.191GlnAla: 5.191 ± 0.931
0.251GlnCys: 0.251 ± 0.167
2.428GlnAsp: 2.428 ± 0.528
3.349GlnGlu: 3.349 ± 0.677
1.758GlnPhe: 1.758 ± 0.341
2.512GlnGly: 2.512 ± 0.463
0.502GlnHis: 0.502 ± 0.181
1.926GlnIle: 1.926 ± 0.392
2.344GlnLys: 2.344 ± 0.451
4.019GlnLeu: 4.019 ± 0.666
0.754GlnMet: 0.754 ± 0.228
1.088GlnAsn: 1.088 ± 0.337
1.088GlnPro: 1.088 ± 0.313
2.009GlnGln: 2.009 ± 0.557
1.926GlnArg: 1.926 ± 0.38
2.763GlnSer: 2.763 ± 0.481
2.595GlnThr: 2.595 ± 0.465
3.516GlnVal: 3.516 ± 0.542
0.586GlnTrp: 0.586 ± 0.261
1.256GlnTyr: 1.256 ± 0.32
0.0GlnXaa: 0.0 ± 0.0
Arg
4.019ArgAla: 4.019 ± 0.504
0.754ArgCys: 0.754 ± 0.295
3.014ArgAsp: 3.014 ± 0.565
3.433ArgGlu: 3.433 ± 0.705
2.344ArgPhe: 2.344 ± 0.381
3.265ArgGly: 3.265 ± 0.537
0.419ArgHis: 0.419 ± 0.191
2.93ArgIle: 2.93 ± 0.536
3.265ArgLys: 3.265 ± 0.557
3.851ArgLeu: 3.851 ± 0.536
0.921ArgMet: 0.921 ± 0.278
2.261ArgAsn: 2.261 ± 0.48
2.093ArgPro: 2.093 ± 0.365
2.344ArgGln: 2.344 ± 0.471
2.344ArgArg: 2.344 ± 0.357
3.014ArgSer: 3.014 ± 0.461
2.428ArgThr: 2.428 ± 0.51
3.265ArgVal: 3.265 ± 0.644
0.67ArgTrp: 0.67 ± 0.21
1.591ArgTyr: 1.591 ± 0.338
0.0ArgXaa: 0.0 ± 0.0
Ser
4.521SerAla: 4.521 ± 0.636
0.67SerCys: 0.67 ± 0.278
4.019SerAsp: 4.019 ± 0.61
3.014SerGlu: 3.014 ± 0.57
2.847SerPhe: 2.847 ± 0.523
6.028SerGly: 6.028 ± 0.803
0.921SerHis: 0.921 ± 0.289
3.684SerIle: 3.684 ± 0.582
4.27SerLys: 4.27 ± 0.641
5.191SerLeu: 5.191 ± 0.486
1.674SerMet: 1.674 ± 0.404
2.679SerAsn: 2.679 ± 0.614
2.009SerPro: 2.009 ± 0.349
1.758SerGln: 1.758 ± 0.349
2.009SerArg: 2.009 ± 0.335
3.433SerSer: 3.433 ± 0.828
3.098SerThr: 3.098 ± 0.565
2.93SerVal: 2.93 ± 0.424
0.419SerTrp: 0.419 ± 0.151
2.512SerTyr: 2.512 ± 0.46
0.0SerXaa: 0.0 ± 0.0
Thr
3.265ThrAla: 3.265 ± 0.612
0.335ThrCys: 0.335 ± 0.191
2.512ThrAsp: 2.512 ± 0.506
4.019ThrGlu: 4.019 ± 0.507
4.186ThrPhe: 4.186 ± 0.8
5.777ThrGly: 5.777 ± 0.731
1.088ThrHis: 1.088 ± 0.256
3.6ThrIle: 3.6 ± 0.586
3.098ThrLys: 3.098 ± 0.737
5.191ThrLeu: 5.191 ± 0.793
1.423ThrMet: 1.423 ± 0.348
2.595ThrAsn: 2.595 ± 0.538
2.93ThrPro: 2.93 ± 0.463
2.512ThrGln: 2.512 ± 0.442
2.595ThrArg: 2.595 ± 0.393
2.595ThrSer: 2.595 ± 0.467
3.768ThrThr: 3.768 ± 0.593
3.768ThrVal: 3.768 ± 0.576
0.67ThrTrp: 0.67 ± 0.272
2.261ThrTyr: 2.261 ± 0.403
0.0ThrXaa: 0.0 ± 0.0
Val
5.442ValAla: 5.442 ± 0.526
0.167ValCys: 0.167 ± 0.129
3.349ValAsp: 3.349 ± 0.411
6.196ValGlu: 6.196 ± 0.878
2.595ValPhe: 2.595 ± 0.471
3.349ValGly: 3.349 ± 0.596
1.423ValHis: 1.423 ± 0.423
3.433ValIle: 3.433 ± 0.62
5.861ValLys: 5.861 ± 0.735
5.61ValLeu: 5.61 ± 0.563
2.093ValMet: 2.093 ± 0.539
3.433ValAsn: 3.433 ± 0.729
2.847ValPro: 2.847 ± 0.446
2.428ValGln: 2.428 ± 0.42
3.433ValArg: 3.433 ± 0.57
3.6ValSer: 3.6 ± 0.564
4.689ValThr: 4.689 ± 0.746
4.605ValVal: 4.605 ± 0.831
0.586ValTrp: 0.586 ± 0.2
2.679ValTyr: 2.679 ± 0.408
0.0ValXaa: 0.0 ± 0.0
Trp
0.921TrpAla: 0.921 ± 0.353
0.251TrpCys: 0.251 ± 0.197
0.837TrpAsp: 0.837 ± 0.304
0.754TrpGlu: 0.754 ± 0.215
0.419TrpPhe: 0.419 ± 0.205
0.502TrpGly: 0.502 ± 0.172
0.419TrpHis: 0.419 ± 0.217
0.67TrpIle: 0.67 ± 0.271
1.172TrpLys: 1.172 ± 0.38
1.256TrpLeu: 1.256 ± 0.396
0.167TrpMet: 0.167 ± 0.112
0.754TrpAsn: 0.754 ± 0.294
0.335TrpPro: 0.335 ± 0.175
0.419TrpGln: 0.419 ± 0.15
0.67TrpArg: 0.67 ± 0.249
1.005TrpSer: 1.005 ± 0.359
1.34TrpThr: 1.34 ± 0.314
1.088TrpVal: 1.088 ± 0.333
0.167TrpTrp: 0.167 ± 0.126
0.586TrpTyr: 0.586 ± 0.284
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.93TyrAla: 2.93 ± 0.611
0.754TyrCys: 0.754 ± 0.229
2.261TyrAsp: 2.261 ± 0.411
2.428TyrGlu: 2.428 ± 0.433
1.842TyrPhe: 1.842 ± 0.469
3.182TyrGly: 3.182 ± 0.491
0.586TyrHis: 0.586 ± 0.234
1.926TyrIle: 1.926 ± 0.472
1.842TyrLys: 1.842 ± 0.407
2.428TyrLeu: 2.428 ± 0.608
1.256TyrMet: 1.256 ± 0.318
1.842TyrAsn: 1.842 ± 0.381
1.842TyrPro: 1.842 ± 0.39
2.177TyrGln: 2.177 ± 0.402
2.009TyrArg: 2.009 ± 0.355
1.591TyrSer: 1.591 ± 0.305
1.842TyrThr: 1.842 ± 0.441
2.428TyrVal: 2.428 ± 0.569
0.586TyrTrp: 0.586 ± 0.242
0.335TyrTyr: 0.335 ± 0.192
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (11945 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski