Giardia muris

Taxonomy: cellular organisms; Eukaryota; Metamonada; Fornicata; Diplomonadida; Hexamitidae; Giardiinae; Giardia

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4560 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z1SNN7|A0A4Z1SNN7_GIAMU PVSP OS=Giardia muris OX=5742 GN=GMRT_24405 PE=4 SV=1
MM1 pKa = 8.02PLTFLRR7 pKa = 11.84TWGEE11 pKa = 4.03VCDD14 pKa = 4.52HH15 pKa = 6.56YY16 pKa = 11.87NNEE19 pKa = 4.04AEE21 pKa = 4.36KK22 pKa = 9.89TFAAIMTIIDD32 pKa = 3.93EE33 pKa = 4.86FCTTMEE39 pKa = 4.81GNDD42 pKa = 3.57SDD44 pKa = 5.03ASLDD48 pKa = 3.66FSDD51 pKa = 3.77FTKK54 pKa = 11.0YY55 pKa = 10.69GAVQSGCQYY64 pKa = 11.3LVIANFGLNVVQFLPFEE81 pKa = 4.46GTVDD85 pKa = 3.67PQSWADD91 pKa = 4.12FIKK94 pKa = 10.08LTEE97 pKa = 4.11SDD99 pKa = 3.59EE100 pKa = 4.14FRR102 pKa = 11.84SLFVV106 pKa = 3.42

Molecular weight: 12.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Z1SLP3|A0A4Z1SLP3_GIAMU Ankyrin repeat protein 2 OS=Giardia muris OX=5742 GN=GMRT_16401 PE=4 SV=1
MM1 pKa = 7.34NPTVKK6 pKa = 10.13VFSATGAPAGEE17 pKa = 4.41LPRR20 pKa = 11.84PAVFSVPIRR29 pKa = 11.84PDD31 pKa = 3.04IISFVHH37 pKa = 5.54TQLAKK42 pKa = 10.87NNRR45 pKa = 11.84TPYY48 pKa = 10.25AVSRR52 pKa = 11.84YY53 pKa = 10.48AGVQCTAHH61 pKa = 5.25SWGPGRR67 pKa = 11.84AVARR71 pKa = 11.84LPRR74 pKa = 11.84KK75 pKa = 9.66HH76 pKa = 6.43GGIGAYY82 pKa = 10.49ANFARR87 pKa = 11.84GGHH90 pKa = 5.33MAHH93 pKa = 6.52PTSVNRR99 pKa = 11.84RR100 pKa = 11.84WCRR103 pKa = 11.84CVNLNLRR110 pKa = 11.84RR111 pKa = 11.84YY112 pKa = 9.15AVASALAASANAQLVEE128 pKa = 4.18ARR130 pKa = 11.84GHH132 pKa = 6.57RR133 pKa = 11.84IGNVKK138 pKa = 10.45SIPCVVDD145 pKa = 3.4VSDD148 pKa = 4.02VKK150 pKa = 10.21KK151 pKa = 9.26TKK153 pKa = 10.53DD154 pKa = 2.97AMGIIRR160 pKa = 11.84AIGAAEE166 pKa = 3.7DD167 pKa = 3.67VEE169 pKa = 4.39RR170 pKa = 11.84CKK172 pKa = 10.62EE173 pKa = 3.97SRR175 pKa = 11.84GIRR178 pKa = 11.84AGRR181 pKa = 11.84GKK183 pKa = 9.34MRR185 pKa = 11.84NRR187 pKa = 11.84RR188 pKa = 11.84YY189 pKa = 10.41RR190 pKa = 11.84MRR192 pKa = 11.84RR193 pKa = 11.84GPLLIHH199 pKa = 6.64AGEE202 pKa = 4.42NIEE205 pKa = 3.96PAFRR209 pKa = 11.84NIPGLDD215 pKa = 3.15ICHH218 pKa = 5.93VSDD221 pKa = 3.89MKK223 pKa = 11.19LLEE226 pKa = 4.54LAPGSHH232 pKa = 7.82PGRR235 pKa = 11.84LIIWTKK241 pKa = 10.69GAFASLDD248 pKa = 3.41QVYY251 pKa = 10.23AAMKK255 pKa = 10.4GYY257 pKa = 7.32TLPMPVISQTDD268 pKa = 3.36IEE270 pKa = 5.57RR271 pKa = 11.84IMQSDD276 pKa = 3.4IVTSTFKK283 pKa = 10.9AKK285 pKa = 10.0RR286 pKa = 11.84DD287 pKa = 3.48PLRR290 pKa = 11.84IEE292 pKa = 4.64RR293 pKa = 11.84KK294 pKa = 9.72VNPFSDD300 pKa = 3.72PNALKK305 pKa = 10.7RR306 pKa = 11.84LDD308 pKa = 3.57PSKK311 pKa = 11.3

Molecular weight: 34.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4560

0

4560

2636066

46

7065

578.1

64.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.468 ± 0.043

2.064 ± 0.03

5.405 ± 0.019

6.614 ± 0.038

3.368 ± 0.028

5.897 ± 0.034

2.36 ± 0.013

5.133 ± 0.024

3.962 ± 0.024

11.318 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.145 ± 0.015

3.221 ± 0.018

5.057 ± 0.034

4.094 ± 0.025

6.74 ± 0.032

7.784 ± 0.036

6.479 ± 0.021

6.119 ± 0.022

0.736 ± 0.009

3.036 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski