Candidatus Berkiella aquae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Coxiellaceae; Candidatus Berkiella

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3170 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q9Z102|A0A0Q9Z102_9COXI Uncharacterized protein OS=Candidatus Berkiella aquae OX=295108 GN=HT99x_00261 PE=4 SV=1
MM1 pKa = 7.39EE2 pKa = 5.86RR3 pKa = 11.84NPEE6 pKa = 4.37RR7 pKa = 11.84IEE9 pKa = 4.11HH10 pKa = 6.24DD11 pKa = 2.87HH12 pKa = 7.42DD13 pKa = 3.64IFVPTVEE20 pKa = 5.01AVFPYY25 pKa = 10.42EE26 pKa = 3.83KK27 pKa = 10.42LAVPFEE33 pKa = 4.13KK34 pKa = 10.67SIDD37 pKa = 3.72SYY39 pKa = 11.79KK40 pKa = 11.03VLDD43 pKa = 3.92EE44 pKa = 4.18EE45 pKa = 5.44ALLKK49 pKa = 10.53VANQTSKK56 pKa = 10.87QSDD59 pKa = 4.01EE60 pKa = 4.42EE61 pKa = 4.82DD62 pKa = 3.43VFAPSLEE69 pKa = 4.05GVFPYY74 pKa = 10.48EE75 pKa = 3.89VLASSFANAMDD86 pKa = 3.95KK87 pKa = 11.17YY88 pKa = 11.08VVLDD92 pKa = 3.88EE93 pKa = 4.54AALLKK98 pKa = 10.14TIEE101 pKa = 4.32DD102 pKa = 3.52AVAKK106 pKa = 10.54SKK108 pKa = 11.28LNANEE113 pKa = 4.31NEE115 pKa = 3.92FLDD118 pKa = 4.67VKK120 pKa = 11.12FEE122 pKa = 4.91DD123 pKa = 4.14LFQIPVTEE131 pKa = 4.18GNAYY135 pKa = 9.03NIKK138 pKa = 8.79MAQYY142 pKa = 8.12MKK144 pKa = 10.07EE145 pKa = 3.84GRR147 pKa = 11.84VEE149 pKa = 4.08SSGSQLEE156 pKa = 4.48VVSVNFDD163 pKa = 3.47YY164 pKa = 11.3QPIEE168 pKa = 3.93MLDD171 pKa = 3.68LVISVPAPQPSITTTTTTNVQSPPTVTTNPQHH203 pKa = 6.56GLPTAPAITMPDD215 pKa = 3.1IFEE218 pKa = 4.29VTLDD222 pKa = 3.58TVAFTTSIGNIFTKK236 pKa = 10.47GASFGPSGGNLVNVYY251 pKa = 9.83FGLPIRR257 pKa = 11.84PGSSEE262 pKa = 3.45IRR264 pKa = 11.84NSDD267 pKa = 3.56VVTLTTAEE275 pKa = 4.06GNEE278 pKa = 3.96LNFYY282 pKa = 10.83LSTFNGHH289 pKa = 6.13QIGDD293 pKa = 3.59YY294 pKa = 10.7EE295 pKa = 4.94YY296 pKa = 9.45ILHH299 pKa = 6.56HH300 pKa = 6.72AVPHH304 pKa = 5.94LLGNPLYY311 pKa = 10.7DD312 pKa = 4.18VIQMGGTKK320 pKa = 9.59IFFDD324 pKa = 3.53NFVYY328 pKa = 10.7SLSNTYY334 pKa = 10.86LEE336 pKa = 4.59TNIGTLTFPIIDD348 pKa = 4.52DD349 pKa = 3.92VPLATSQNGGTISEE363 pKa = 4.17AAIEE367 pKa = 4.16NSGTDD372 pKa = 3.51QSHH375 pKa = 5.52VPALLSGTLINPPVDD390 pKa = 3.02RR391 pKa = 11.84FGADD395 pKa = 2.84GGTVTSVTINGGSYY409 pKa = 10.87GFLFGHH415 pKa = 6.35IVVITTEE422 pKa = 4.35GNILTVNVNTGAYY435 pKa = 7.88TFLLSNPLHH444 pKa = 5.76NTNNQPINQVFTYY457 pKa = 10.68VFTDD461 pKa = 3.09SDD463 pKa = 3.95GSTASNTLTITIDD476 pKa = 3.54DD477 pKa = 5.74DD478 pKa = 4.15IPIATEE484 pKa = 3.81KK485 pKa = 10.51TNTASEE491 pKa = 4.37TLCFVNGSDD500 pKa = 3.36TATGNLISDD509 pKa = 4.2DD510 pKa = 3.91NGFGISLFGADD521 pKa = 4.52GGDD524 pKa = 2.9ITAVNGVTDD533 pKa = 4.01ASDD536 pKa = 3.62GLVDD540 pKa = 5.35GIIHH544 pKa = 7.22APTTFGDD551 pKa = 3.42IEE553 pKa = 4.88VYY555 pKa = 10.41VAVQSGHH562 pKa = 5.95QIGDD566 pKa = 3.61YY567 pKa = 10.24IYY569 pKa = 10.53TLDD572 pKa = 3.63TSKK575 pKa = 9.51TAPQNDD581 pKa = 3.54NLITVLDD588 pKa = 4.54TISYY592 pKa = 8.1TITDD596 pKa = 3.49NDD598 pKa = 3.8GSQDD602 pKa = 3.51SANLVVTVTLNQAPTAVDD620 pKa = 3.78DD621 pKa = 5.0VGTTDD626 pKa = 3.85EE627 pKa = 4.16NTILDD632 pKa = 4.09VLTVNGVLSNDD643 pKa = 3.44TDD645 pKa = 4.32PNPGDD650 pKa = 3.68TKK652 pKa = 10.61IVSAVNGLGTNVGNQFTLLSNALLLLNADD681 pKa = 3.58GSYY684 pKa = 10.03TYY686 pKa = 11.04NPNGVFNYY694 pKa = 9.8LAAGSQGIDD703 pKa = 2.79SFTYY707 pKa = 9.75TMHH710 pKa = 7.28DD711 pKa = 3.45AEE713 pKa = 5.06GLSSSATVTITINGVNSAPVAVDD736 pKa = 4.0DD737 pKa = 5.91SNTTDD742 pKa = 3.25ANAIINVNTIVDD754 pKa = 3.73PHH756 pKa = 6.93NLLINDD762 pKa = 3.93TDD764 pKa = 4.15DD765 pKa = 3.99VGDD768 pKa = 3.51THH770 pKa = 7.64SISEE774 pKa = 4.44VNGVAANVGNQITLASGALLTVNADD799 pKa = 2.79GTYY802 pKa = 10.53NYY804 pKa = 10.13NPNHH808 pKa = 6.1MFDD811 pKa = 4.29SLAQGSSTTDD821 pKa = 2.61SFTYY825 pKa = 9.93TLQDD829 pKa = 3.13SGGLTSTATVIITINGVNDD848 pKa = 3.38APTAVDD854 pKa = 4.96DD855 pKa = 5.7SNTTDD860 pKa = 3.71ANTMIDD866 pKa = 3.69VNLVSDD872 pKa = 3.97PHH874 pKa = 7.33NLLINDD880 pKa = 4.08TDD882 pKa = 4.38PDD884 pKa = 3.78VGDD887 pKa = 3.55TLVISEE893 pKa = 4.41VQGLAANVGVQITLASGALLTVNANGTYY921 pKa = 10.02QYY923 pKa = 10.47NPNGAFVSLSVGASTIDD940 pKa = 3.22SFTYY944 pKa = 9.54TVQDD948 pKa = 3.29SGGLTSTATVSITINGVNEE967 pKa = 3.72APIAVDD973 pKa = 4.93DD974 pKa = 4.52SNTTHH979 pKa = 6.23GTTVIDD985 pKa = 3.92VNSTLDD991 pKa = 3.73PQCLLFNDD999 pKa = 4.45SDD1001 pKa = 4.72PDD1003 pKa = 3.68TGDD1006 pKa = 3.06TFVISEE1012 pKa = 4.52VEE1014 pKa = 3.98GQAANVGVQITLTSGALLTVNADD1037 pKa = 2.8GTYY1040 pKa = 10.1QYY1042 pKa = 11.43DD1043 pKa = 3.81SNGVFAATDD1052 pKa = 3.37IDD1054 pKa = 4.11SFTYY1058 pKa = 10.41KK1059 pKa = 9.24ITDD1062 pKa = 3.37NHH1064 pKa = 6.27GLTSNAATVTINVIVPPIILDD1085 pKa = 4.01LNDD1088 pKa = 4.71DD1089 pKa = 5.24GINLIPPDD1097 pKa = 3.67EE1098 pKa = 4.22SQIAFSLFGRR1108 pKa = 11.84EE1109 pKa = 3.74NTTTIGWVNGQDD1121 pKa = 3.24GLLAIDD1127 pKa = 4.6LNGDD1131 pKa = 3.65KK1132 pKa = 10.32IINGLEE1138 pKa = 4.05EE1139 pKa = 4.16FTFTHH1144 pKa = 6.85PNAKK1148 pKa = 8.87TDD1150 pKa = 3.98LEE1152 pKa = 4.16ALRR1155 pKa = 11.84LLYY1158 pKa = 10.52DD1159 pKa = 3.82SNVDD1163 pKa = 4.77GILDD1167 pKa = 3.68MQDD1170 pKa = 3.08EE1171 pKa = 4.33DD1172 pKa = 3.48WARR1175 pKa = 11.84FGVWQDD1181 pKa = 2.99ANEE1184 pKa = 4.08NGICEE1189 pKa = 4.23PGEE1192 pKa = 3.89FLSLAEE1198 pKa = 4.56RR1199 pKa = 11.84GIASIDD1205 pKa = 4.23LISDD1209 pKa = 3.43QQSDD1213 pKa = 4.25VIAGNIIFGYY1223 pKa = 8.9ATYY1226 pKa = 8.71QTTDD1230 pKa = 3.11GQLHH1234 pKa = 5.2QLADD1238 pKa = 3.47VGLGLFF1244 pKa = 4.27

Molecular weight: 131.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q9YZE6|A0A0Q9YZE6_9COXI Methylmalonyl-CoA carboxyltransferase 12S subunit OS=Candidatus Berkiella aquae OX=295108 GN=HT99x_00826 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.36RR3 pKa = 11.84PFQRR7 pKa = 11.84KK8 pKa = 7.04SQVRR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84THH17 pKa = 6.09GFRR20 pKa = 11.84QRR22 pKa = 11.84MQTKK26 pKa = 9.63GGRR29 pKa = 11.84AVLANRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.99GRR40 pKa = 11.84KK41 pKa = 8.45RR42 pKa = 11.84LAPVGGTKK50 pKa = 10.05KK51 pKa = 10.67

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3170

0

3170

1075553

29

4642

339.3

38.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.282 ± 0.046

1.096 ± 0.019

4.886 ± 0.04

6.174 ± 0.053

4.212 ± 0.035

5.889 ± 0.052

2.563 ± 0.024

7.135 ± 0.042

6.527 ± 0.063

10.725 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.44 ± 0.02

4.763 ± 0.038

4.253 ± 0.033

4.995 ± 0.043

4.202 ± 0.037

6.302 ± 0.037

5.41 ± 0.042

5.898 ± 0.039

1.078 ± 0.016

3.169 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski