Amino acid dipepetide frequency for Herbinix hemicellulosilytica

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.162AlaAla: 5.162 ± 0.096	0.797AlaCys: 0.797 ± 0.037	3.762AlaAsp: 3.762 ± 0.064	4.298AlaGlu: 4.298 ± 0.076	2.712AlaPhe: 2.712 ± 0.061	4.81AlaGly: 4.81 ± 0.09	0.88AlaHis: 0.88 ± 0.034	5.458AlaIle: 5.458 ± 0.08	5.033AlaLys: 5.033 ± 0.078	6.182AlaLeu: 6.182 ± 0.09	1.685AlaMet: 1.685 ± 0.044	2.746AlaAsn: 2.746 ± 0.06	1.552AlaPro: 1.552 ± 0.048	1.754AlaGln: 1.754 ± 0.049	2.574AlaArg: 2.574 ± 0.058	3.399AlaSer: 3.399 ± 0.066	2.901AlaThr: 2.901 ± 0.074	5.166AlaVal: 5.166 ± 0.079	0.454AlaTrp: 0.454 ± 0.025	2.77AlaTyr: 2.77 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	0.654CysAla: 0.654 ± 0.027	0.216CysCys: 0.216 ± 0.016	0.73CysAsp: 0.73 ± 0.034	0.752CysGlu: 0.752 ± 0.034	0.549CysPhe: 0.549 ± 0.025	1.11CysGly: 1.11 ± 0.043	0.248CysHis: 0.248 ± 0.016	1.09CysIle: 1.09 ± 0.033	0.767CysLys: 0.767 ± 0.035	0.974CysLeu: 0.974 ± 0.034	0.29CysMet: 0.29 ± 0.021	0.618CysAsn: 0.618 ± 0.026	0.528CysPro: 0.528 ± 0.031	0.255CysGln: 0.255 ± 0.016	0.513CysArg: 0.513 ± 0.027	0.72CysSer: 0.72 ± 0.029	0.546CysThr: 0.546 ± 0.025	0.711CysVal: 0.711 ± 0.027	0.084CysTrp: 0.084 ± 0.01	0.487CysTyr: 0.487 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	3.241AspAla: 3.241 ± 0.062	0.658AspCys: 0.658 ± 0.026	3.147AspAsp: 3.147 ± 0.08	4.951AspGlu: 4.951 ± 0.099	2.82AspPhe: 2.82 ± 0.061	3.521AspGly: 3.521 ± 0.076	0.725AspHis: 0.725 ± 0.03	6.013AspIle: 6.013 ± 0.094	4.836AspLys: 4.836 ± 0.091	5.147AspLeu: 5.147 ± 0.08	1.73AspMet: 1.73 ± 0.042	3.253AspAsn: 3.253 ± 0.077	1.819AspPro: 1.819 ± 0.051	1.17AspGln: 1.17 ± 0.043	2.441AspArg: 2.441 ± 0.05	2.947AspSer: 2.947 ± 0.063	2.964AspThr: 2.964 ± 0.067	3.504AspVal: 3.504 ± 0.066	0.546AspTrp: 0.546 ± 0.032	3.29AspTyr: 3.29 ± 0.062	0.0AspXaa: 0.0 ± 0.0
Glu	5.092GluAla: 5.092 ± 0.084	0.64GluCys: 0.64 ± 0.026	4.221GluAsp: 4.221 ± 0.09	6.428GluGlu: 6.428 ± 0.109	2.873GluPhe: 2.873 ± 0.065	4.012GluGly: 4.012 ± 0.066	1.11GluHis: 1.11 ± 0.038	6.654GluIle: 6.654 ± 0.105	6.506GluLys: 6.506 ± 0.098	6.742GluLeu: 6.742 ± 0.098	1.878GluMet: 1.878 ± 0.048	4.658GluAsn: 4.658 ± 0.08	1.749GluPro: 1.749 ± 0.045	2.131GluGln: 2.131 ± 0.05	3.104GluArg: 3.104 ± 0.067	3.064GluSer: 3.064 ± 0.063	3.33GluThr: 3.33 ± 0.067	4.304GluVal: 4.304 ± 0.081	0.579GluTrp: 0.579 ± 0.027	3.555GluTyr: 3.555 ± 0.073	0.0GluXaa: 0.0 ± 0.0
Phe	2.592PheAla: 2.592 ± 0.054	0.588PheCys: 0.588 ± 0.026	2.71PheAsp: 2.71 ± 0.059	2.858PheGlu: 2.858 ± 0.057	1.874PhePhe: 1.874 ± 0.061	2.904PheGly: 2.904 ± 0.069	0.611PheHis: 0.611 ± 0.024	4.145PheIle: 4.145 ± 0.074	2.84PheLys: 2.84 ± 0.064	3.788PheLeu: 3.788 ± 0.079	1.139PheMet: 1.139 ± 0.035	2.268PheAsn: 2.268 ± 0.06	1.302PhePro: 1.302 ± 0.041	0.944PheGln: 0.944 ± 0.031	1.573PheArg: 1.573 ± 0.041	2.848PheSer: 2.848 ± 0.067	2.39PheThr: 2.39 ± 0.055	2.698PheVal: 2.698 ± 0.064	0.405PheTrp: 0.405 ± 0.022	2.072PheTyr: 2.072 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	3.839GlyAla: 3.839 ± 0.085	0.979GlyCys: 0.979 ± 0.04	3.179GlyAsp: 3.179 ± 0.073	3.9GlyGlu: 3.9 ± 0.075	3.061GlyPhe: 3.061 ± 0.068	3.959GlyGly: 3.959 ± 0.087	1.065GlyHis: 1.065 ± 0.038	6.468GlyIle: 6.468 ± 0.103	5.498GlyLys: 5.498 ± 0.077	5.758GlyLeu: 5.758 ± 0.089	1.927GlyMet: 1.927 ± 0.053	3.18GlyAsn: 3.18 ± 0.081	1.361GlyPro: 1.361 ± 0.046	1.762GlyGln: 1.762 ± 0.049	2.736GlyArg: 2.736 ± 0.066	3.564GlySer: 3.564 ± 0.072	3.565GlyThr: 3.565 ± 0.077	4.144GlyVal: 4.144 ± 0.084	0.533GlyTrp: 0.533 ± 0.026	3.343GlyTyr: 3.343 ± 0.065	0.0GlyXaa: 0.0 ± 0.0
His	0.859HisAla: 0.859 ± 0.031	0.218HisCys: 0.218 ± 0.015	0.829HisAsp: 0.829 ± 0.034	0.943HisGlu: 0.943 ± 0.034	0.698HisPhe: 0.698 ± 0.028	1.057HisGly: 1.057 ± 0.036	0.306HisHis: 0.306 ± 0.019	1.496HisIle: 1.496 ± 0.044	1.093HisLys: 1.093 ± 0.04	1.306HisLeu: 1.306 ± 0.044	0.394HisMet: 0.394 ± 0.022	0.833HisAsn: 0.833 ± 0.032	0.743HisPro: 0.743 ± 0.032	0.415HisGln: 0.415 ± 0.022	0.651HisArg: 0.651 ± 0.028	0.824HisSer: 0.824 ± 0.029	0.807HisThr: 0.807 ± 0.031	0.837HisVal: 0.837 ± 0.031	0.153HisTrp: 0.153 ± 0.014	0.721HisTyr: 0.721 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.804IleAla: 5.804 ± 0.088	1.273IleCys: 1.273 ± 0.043	5.325IleAsp: 5.325 ± 0.078	6.154IleGlu: 6.154 ± 0.105	3.724IlePhe: 3.724 ± 0.082	5.613IleGly: 5.613 ± 0.082	1.285IleHis: 1.285 ± 0.042	8.316IleIle: 8.316 ± 0.116	7.32IleLys: 7.32 ± 0.086	8.402IleLeu: 8.402 ± 0.128	2.268IleMet: 2.268 ± 0.049	5.297IleAsn: 5.297 ± 0.092	3.559IlePro: 3.559 ± 0.069	2.153IleGln: 2.153 ± 0.051	3.987IleArg: 3.987 ± 0.065	6.201IleSer: 6.201 ± 0.099	5.035IleThr: 5.035 ± 0.08	5.239IleVal: 5.239 ± 0.098	0.593IleTrp: 0.593 ± 0.028	3.854IleTyr: 3.854 ± 0.07	0.0IleXaa: 0.0 ± 0.0
Lys	5.387LysAla: 5.387 ± 0.088	0.631LysCys: 0.631 ± 0.029	5.161LysAsp: 5.161 ± 0.083	7.746LysGlu: 7.746 ± 0.119	2.455LysPhe: 2.455 ± 0.055	4.29LysGly: 4.29 ± 0.071	1.144LysHis: 1.144 ± 0.033	6.848LysIle: 6.848 ± 0.095	7.207LysLys: 7.207 ± 0.116	6.772LysLeu: 6.772 ± 0.083	2.048LysMet: 2.048 ± 0.047	5.057LysAsn: 5.057 ± 0.082	2.481LysPro: 2.481 ± 0.054	2.266LysGln: 2.266 ± 0.042	3.325LysArg: 3.325 ± 0.063	4.176LysSer: 4.176 ± 0.074	4.047LysThr: 4.047 ± 0.063	4.75LysVal: 4.75 ± 0.084	0.636LysTrp: 0.636 ± 0.029	3.769LysTyr: 3.769 ± 0.066	0.0LysXaa: 0.0 ± 0.0
Leu	6.003LeuAla: 6.003 ± 0.092	1.142LeuCys: 1.142 ± 0.038	5.078LeuAsp: 5.078 ± 0.082	5.866LeuGlu: 5.866 ± 0.095	3.835LeuPhe: 3.835 ± 0.091	5.618LeuGly: 5.618 ± 0.083	1.36LeuHis: 1.36 ± 0.038	8.107LeuIle: 8.107 ± 0.125	7.663LeuLys: 7.663 ± 0.106	8.638LeuLeu: 8.638 ± 0.137	2.422LeuMet: 2.422 ± 0.059	5.054LeuAsn: 5.054 ± 0.078	3.4LeuPro: 3.4 ± 0.06	2.291LeuGln: 2.291 ± 0.061	3.691LeuArg: 3.691 ± 0.066	6.621LeuSer: 6.621 ± 0.094	4.819LeuThr: 4.819 ± 0.079	5.184LeuVal: 5.184 ± 0.079	0.731LeuTrp: 0.731 ± 0.028	3.827LeuTyr: 3.827 ± 0.07	0.0LeuXaa: 0.0 ± 0.0
Met	2.087MetAla: 2.087 ± 0.052	0.216MetCys: 0.216 ± 0.017	1.663MetAsp: 1.663 ± 0.047	1.919MetGlu: 1.919 ± 0.044	0.969MetPhe: 0.969 ± 0.032	1.705MetGly: 1.705 ± 0.048	0.414MetHis: 0.414 ± 0.02	2.166MetIle: 2.166 ± 0.052	2.288MetLys: 2.288 ± 0.051	2.374MetLeu: 2.374 ± 0.059	0.713MetMet: 0.713 ± 0.028	1.539MetAsn: 1.539 ± 0.04	1.029MetPro: 1.029 ± 0.036	0.837MetGln: 0.837 ± 0.032	1.029MetArg: 1.029 ± 0.041	1.467MetSer: 1.467 ± 0.043	1.368MetThr: 1.368 ± 0.039	1.716MetVal: 1.716 ± 0.047	0.17MetTrp: 0.17 ± 0.014	0.89MetTyr: 0.89 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	3.067AsnAla: 3.067 ± 0.065	0.59AsnCys: 0.59 ± 0.025	2.91AsnAsp: 2.91 ± 0.061	3.845AsnGlu: 3.845 ± 0.068	2.093AsnPhe: 2.093 ± 0.049	3.463AsnGly: 3.463 ± 0.08	0.902AsnHis: 0.902 ± 0.038	5.7AsnIle: 5.7 ± 0.083	4.315AsnLys: 4.315 ± 0.078	5.022AsnLeu: 5.022 ± 0.075	1.543AsnMet: 1.543 ± 0.037	3.324AsnAsn: 3.324 ± 0.074	2.243AsnPro: 2.243 ± 0.057	1.527AsnGln: 1.527 ± 0.043	2.341AsnArg: 2.341 ± 0.063	2.991AsnSer: 2.991 ± 0.062	2.846AsnThr: 2.846 ± 0.066	3.182AsnVal: 3.182 ± 0.068	0.448AsnTrp: 0.448 ± 0.023	2.509AsnTyr: 2.509 ± 0.054	0.0AsnXaa: 0.0 ± 0.0
Pro	2.103ProAla: 2.103 ± 0.055	0.356ProCys: 0.356 ± 0.024	2.43ProAsp: 2.43 ± 0.057	2.843ProGlu: 2.843 ± 0.06	1.565ProPhe: 1.565 ± 0.042	2.202ProGly: 2.202 ± 0.049	0.543ProHis: 0.543 ± 0.026	2.437ProIle: 2.437 ± 0.056	2.226ProLys: 2.226 ± 0.053	2.619ProLeu: 2.619 ± 0.052	0.798ProMet: 0.798 ± 0.032	1.56ProAsn: 1.56 ± 0.047	0.845ProPro: 0.845 ± 0.034	0.889ProGln: 0.889 ± 0.034	0.976ProArg: 0.976 ± 0.043	1.74ProSer: 1.74 ± 0.05	1.553ProThr: 1.553 ± 0.06	2.713ProVal: 2.713 ± 0.062	0.292ProTrp: 0.292 ± 0.02	1.603ProTyr: 1.603 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	1.939GlnAla: 1.939 ± 0.053	0.224GlnCys: 0.224 ± 0.014	1.372GlnAsp: 1.372 ± 0.042	1.826GlnGlu: 1.826 ± 0.053	1.016GlnPhe: 1.016 ± 0.032	1.566GlnGly: 1.566 ± 0.043	0.324GlnHis: 0.324 ± 0.019	2.519GlnIle: 2.519 ± 0.063	2.255GlnLys: 2.255 ± 0.06	2.503GlnLeu: 2.503 ± 0.057	0.83GlnMet: 0.83 ± 0.033	1.515GlnAsn: 1.515 ± 0.049	0.719GlnPro: 0.719 ± 0.031	0.768GlnGln: 0.768 ± 0.036	1.115GlnArg: 1.115 ± 0.036	1.347GlnSer: 1.347 ± 0.044	1.372GlnThr: 1.372 ± 0.043	1.688GlnVal: 1.688 ± 0.042	0.236GlnTrp: 0.236 ± 0.02	1.174GlnTyr: 1.174 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	2.209ArgAla: 2.209 ± 0.045	0.495ArgCys: 0.495 ± 0.022	2.117ArgAsp: 2.117 ± 0.049	3.207ArgGlu: 3.207 ± 0.075	1.848ArgPhe: 1.848 ± 0.039	2.103ArgGly: 2.103 ± 0.055	0.702ArgHis: 0.702 ± 0.031	3.999ArgIle: 3.999 ± 0.066	3.543ArgLys: 3.543 ± 0.07	4.007ArgLeu: 4.007 ± 0.068	1.274ArgMet: 1.274 ± 0.038	2.251ArgAsn: 2.251 ± 0.046	1.232ArgPro: 1.232 ± 0.041	1.373ArgGln: 1.373 ± 0.044	1.807ArgArg: 1.807 ± 0.06	1.942ArgSer: 1.942 ± 0.046	2.045ArgThr: 2.045 ± 0.052	2.495ArgVal: 2.495 ± 0.067	0.331ArgTrp: 0.331 ± 0.022	1.968ArgTyr: 1.968 ± 0.056	0.0ArgXaa: 0.0 ± 0.0
Ser	3.398SerAla: 3.398 ± 0.069	0.668SerCys: 0.668 ± 0.029	3.615SerAsp: 3.615 ± 0.071	3.777SerGlu: 3.777 ± 0.071	2.823SerPhe: 2.823 ± 0.069	4.347SerGly: 4.347 ± 0.064	0.896SerHis: 0.896 ± 0.029	4.93SerIle: 4.93 ± 0.09	4.562SerLys: 4.562 ± 0.074	5.366SerLeu: 5.366 ± 0.093	1.483SerMet: 1.483 ± 0.042	2.908SerAsn: 2.908 ± 0.077	1.753SerPro: 1.753 ± 0.045	1.552SerGln: 1.552 ± 0.046	2.327SerArg: 2.327 ± 0.053	3.559SerSer: 3.559 ± 0.086	2.727SerThr: 2.727 ± 0.061	3.953SerVal: 3.953 ± 0.077	0.423SerTrp: 0.423 ± 0.023	2.6SerTyr: 2.6 ± 0.06	0.0SerXaa: 0.0 ± 0.0
Thr	3.578ThrAla: 3.578 ± 0.073	0.527ThrCys: 0.527 ± 0.027	3.43ThrAsp: 3.43 ± 0.071	3.447ThrGlu: 3.447 ± 0.064	2.073ThrPhe: 2.073 ± 0.057	4.42ThrGly: 4.42 ± 0.086	0.794ThrHis: 0.794 ± 0.035	4.137ThrIle: 4.137 ± 0.081	3.28ThrLys: 3.28 ± 0.065	4.693ThrLeu: 4.693 ± 0.077	1.108ThrMet: 1.108 ± 0.035	2.228ThrAsn: 2.228 ± 0.063	2.006ThrPro: 2.006 ± 0.074	1.261ThrGln: 1.261 ± 0.04	1.769ThrArg: 1.769 ± 0.048	2.797ThrSer: 2.797 ± 0.065	2.51ThrThr: 2.51 ± 0.076	4.376ThrVal: 4.376 ± 0.083	0.424ThrTrp: 0.424 ± 0.026	2.377ThrTyr: 2.377 ± 0.071	0.0ThrXaa: 0.0 ± 0.0
Val	4.017ValAla: 4.017 ± 0.077	0.898ValCys: 0.898 ± 0.03	3.6ValAsp: 3.6 ± 0.075	4.05ValGlu: 4.05 ± 0.08	3.011ValPhe: 3.011 ± 0.061	3.642ValGly: 3.642 ± 0.082	0.879ValHis: 0.879 ± 0.034	6.227ValIle: 6.227 ± 0.099	4.95ValLys: 4.95 ± 0.08	5.993ValLeu: 5.993 ± 0.082	1.609ValMet: 1.609 ± 0.047	3.446ValAsn: 3.446 ± 0.064	2.203ValPro: 2.203 ± 0.054	1.419ValGln: 1.419 ± 0.042	2.555ValArg: 2.555 ± 0.059	4.278ValSer: 4.278 ± 0.065	3.691ValThr: 3.691 ± 0.079	4.151ValVal: 4.151 ± 0.083	0.488ValTrp: 0.488 ± 0.023	2.85ValTyr: 2.85 ± 0.056	0.0ValXaa: 0.0 ± 0.0
Trp	0.484TrpAla: 0.484 ± 0.028	0.116TrpCys: 0.116 ± 0.013	0.529TrpAsp: 0.529 ± 0.027	0.54TrpGlu: 0.54 ± 0.027	0.402TrpPhe: 0.402 ± 0.021	0.542TrpGly: 0.542 ± 0.025	0.156TrpHis: 0.156 ± 0.015	0.621TrpIle: 0.621 ± 0.026	0.563TrpLys: 0.563 ± 0.029	0.816TrpLeu: 0.816 ± 0.031	0.257TrpMet: 0.257 ± 0.019	0.462TrpAsn: 0.462 ± 0.026	0.197TrpPro: 0.197 ± 0.019	0.289TrpGln: 0.289 ± 0.018	0.303TrpArg: 0.303 ± 0.018	0.488TrpSer: 0.488 ± 0.03	0.354TrpThr: 0.354 ± 0.024	0.429TrpVal: 0.429 ± 0.025	0.08TrpTrp: 0.08 ± 0.01	0.343TrpTyr: 0.343 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.581TyrAla: 2.581 ± 0.047	0.608TyrCys: 0.608 ± 0.031	2.917TyrAsp: 2.917 ± 0.076	3.28TyrGlu: 3.28 ± 0.069	2.146TyrPhe: 2.146 ± 0.054	2.937TyrGly: 2.937 ± 0.063	0.837TyrHis: 0.837 ± 0.034	4.07TyrIle: 4.07 ± 0.071	3.452TyrLys: 3.452 ± 0.071	4.249TyrLeu: 4.249 ± 0.081	1.163TyrMet: 1.163 ± 0.04	2.665TyrAsn: 2.665 ± 0.064	1.573TyrPro: 1.573 ± 0.047	1.249TyrGln: 1.249 ± 0.04	2.132TyrArg: 2.132 ± 0.047	2.693TyrSer: 2.693 ± 0.065	2.364TyrThr: 2.364 ± 0.056	2.719TyrVal: 2.719 ± 0.06	0.364TyrTrp: 0.364 ± 0.023	2.403TyrTyr: 2.403 ± 0.071	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2640 proteins (848450 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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