Amino acid dipepetide frequency for Enterobacteria phage KhF2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.096AlaAla: 5.096 ± 0.704
0.485AlaCys: 0.485 ± 0.18
4.271AlaAsp: 4.271 ± 0.466
4.223AlaGlu: 4.223 ± 0.435
3.009AlaPhe: 3.009 ± 0.45
5.145AlaGly: 5.145 ± 0.653
1.602AlaHis: 1.602 ± 0.31
4.417AlaIle: 4.417 ± 0.434
5.533AlaLys: 5.533 ± 0.59
5.873AlaLeu: 5.873 ± 0.62
2.767AlaMet: 2.767 ± 0.381
4.126AlaAsn: 4.126 ± 0.539
1.844AlaPro: 1.844 ± 0.332
3.009AlaGln: 3.009 ± 0.437
2.233AlaArg: 2.233 ± 0.358
4.368AlaSer: 4.368 ± 0.599
4.271AlaThr: 4.271 ± 0.432
5.873AlaVal: 5.873 ± 0.596
0.922AlaTrp: 0.922 ± 0.203
3.106AlaTyr: 3.106 ± 0.382
0.0AlaXaa: 0.0 ± 0.0
Cys
0.485CysAla: 0.485 ± 0.221
0.194CysCys: 0.194 ± 0.118
0.291CysAsp: 0.291 ± 0.12
1.116CysGlu: 1.116 ± 0.26
0.631CysPhe: 0.631 ± 0.169
1.068CysGly: 1.068 ± 0.257
0.34CysHis: 0.34 ± 0.144
0.437CysIle: 0.437 ± 0.143
1.165CysLys: 1.165 ± 0.27
0.971CysLeu: 0.971 ± 0.199
0.437CysMet: 0.437 ± 0.142
0.631CysAsn: 0.631 ± 0.167
0.582CysPro: 0.582 ± 0.205
0.485CysGln: 0.485 ± 0.164
0.582CysArg: 0.582 ± 0.218
0.874CysSer: 0.874 ± 0.238
0.534CysThr: 0.534 ± 0.154
0.825CysVal: 0.825 ± 0.216
0.097CysTrp: 0.097 ± 0.075
0.582CysTyr: 0.582 ± 0.162
0.0CysXaa: 0.0 ± 0.0
Asp
4.465AspAla: 4.465 ± 0.42
0.631AspCys: 0.631 ± 0.173
3.543AspAsp: 3.543 ± 0.465
4.417AspGlu: 4.417 ± 0.525
3.252AspPhe: 3.252 ± 0.367
4.611AspGly: 4.611 ± 0.503
0.777AspHis: 0.777 ± 0.225
4.174AspIle: 4.174 ± 0.487
4.223AspLys: 4.223 ± 0.399
5.193AspLeu: 5.193 ± 0.49
1.456AspMet: 1.456 ± 0.273
3.883AspAsn: 3.883 ± 0.52
1.796AspPro: 1.796 ± 0.356
0.68AspGln: 0.68 ± 0.193
2.184AspArg: 2.184 ± 0.24
4.223AspSer: 4.223 ± 0.456
3.98AspThr: 3.98 ± 0.378
4.757AspVal: 4.757 ± 0.542
1.31AspTrp: 1.31 ± 0.314
2.864AspTyr: 2.864 ± 0.395
0.0AspXaa: 0.0 ± 0.0
Glu
6.116GluAla: 6.116 ± 0.72
0.631GluCys: 0.631 ± 0.185
4.126GluAsp: 4.126 ± 0.537
5.727GluGlu: 5.727 ± 0.648
3.058GluPhe: 3.058 ± 0.349
4.077GluGly: 4.077 ± 0.417
1.65GluHis: 1.65 ± 0.345
4.32GluIle: 4.32 ± 0.552
4.951GluLys: 4.951 ± 0.7
6.164GluLeu: 6.164 ± 0.511
2.621GluMet: 2.621 ± 0.317
3.446GluAsn: 3.446 ± 0.344
1.602GluPro: 1.602 ± 0.31
2.281GluGln: 2.281 ± 0.393
3.106GluArg: 3.106 ± 0.338
3.98GluSer: 3.98 ± 0.454
3.349GluThr: 3.349 ± 0.358
4.611GluVal: 4.611 ± 0.412
0.728GluTrp: 0.728 ± 0.211
2.378GluTyr: 2.378 ± 0.396
0.0GluXaa: 0.0 ± 0.0
Phe
2.864PheAla: 2.864 ± 0.391
0.388PheCys: 0.388 ± 0.138
3.155PheAsp: 3.155 ± 0.344
3.252PheGlu: 3.252 ± 0.38
1.068PhePhe: 1.068 ± 0.227
3.203PheGly: 3.203 ± 0.378
0.825PheHis: 0.825 ± 0.236
2.621PheIle: 2.621 ± 0.389
3.834PheLys: 3.834 ± 0.413
2.815PheLeu: 2.815 ± 0.4
1.019PheMet: 1.019 ± 0.225
2.427PheAsn: 2.427 ± 0.34
1.116PhePro: 1.116 ± 0.273
1.456PheGln: 1.456 ± 0.233
1.262PheArg: 1.262 ± 0.189
2.767PheSer: 2.767 ± 0.329
2.524PheThr: 2.524 ± 0.364
2.961PheVal: 2.961 ± 0.337
0.485PheTrp: 0.485 ± 0.146
1.844PheTyr: 1.844 ± 0.287
0.0PheXaa: 0.0 ± 0.0
Gly
4.708GlyAla: 4.708 ± 0.495
1.165GlyCys: 1.165 ± 0.274
3.398GlyAsp: 3.398 ± 0.386
4.465GlyGlu: 4.465 ± 0.558
3.349GlyPhe: 3.349 ± 0.418
4.465GlyGly: 4.465 ± 0.64
1.359GlyHis: 1.359 ± 0.246
4.66GlyIle: 4.66 ± 0.486
5.679GlyLys: 5.679 ± 0.625
5.048GlyLeu: 5.048 ± 0.483
1.116GlyMet: 1.116 ± 0.235
2.961GlyAsn: 2.961 ± 0.452
0.097GlyPro: 0.097 ± 0.073
2.281GlyGln: 2.281 ± 0.462
2.718GlyArg: 2.718 ± 0.354
4.126GlySer: 4.126 ± 0.522
4.174GlyThr: 4.174 ± 0.502
5.388GlyVal: 5.388 ± 0.457
0.971GlyTrp: 0.971 ± 0.253
2.912GlyTyr: 2.912 ± 0.421
0.0GlyXaa: 0.0 ± 0.0
His
0.971HisAla: 0.971 ± 0.183
0.388HisCys: 0.388 ± 0.157
1.068HisAsp: 1.068 ± 0.248
1.213HisGlu: 1.213 ± 0.215
0.874HisPhe: 0.874 ± 0.19
0.777HisGly: 0.777 ± 0.215
0.485HisHis: 0.485 ± 0.163
0.922HisIle: 0.922 ± 0.225
0.971HisLys: 0.971 ± 0.25
1.893HisLeu: 1.893 ± 0.355
0.631HisMet: 0.631 ± 0.177
1.262HisAsn: 1.262 ± 0.356
0.825HisPro: 0.825 ± 0.187
0.485HisGln: 0.485 ± 0.133
0.874HisArg: 0.874 ± 0.199
1.65HisSer: 1.65 ± 0.341
1.505HisThr: 1.505 ± 0.447
1.553HisVal: 1.553 ± 0.274
0.291HisTrp: 0.291 ± 0.157
0.874HisTyr: 0.874 ± 0.194
0.0HisXaa: 0.0 ± 0.0
Ile
4.126IleAla: 4.126 ± 0.435
1.019IleCys: 1.019 ± 0.264
4.223IleAsp: 4.223 ± 0.413
4.368IleGlu: 4.368 ± 0.49
2.475IlePhe: 2.475 ± 0.314
3.446IleGly: 3.446 ± 0.46
1.165IleHis: 1.165 ± 0.204
3.3IleIle: 3.3 ± 0.4
5.193IleLys: 5.193 ± 0.637
3.834IleLeu: 3.834 ± 0.485
1.456IleMet: 1.456 ± 0.284
3.155IleAsn: 3.155 ± 0.339
2.136IlePro: 2.136 ± 0.417
1.747IleGln: 1.747 ± 0.284
2.815IleArg: 2.815 ± 0.36
4.077IleSer: 4.077 ± 0.433
3.883IleThr: 3.883 ± 0.449
3.64IleVal: 3.64 ± 0.462
0.534IleTrp: 0.534 ± 0.149
3.058IleTyr: 3.058 ± 0.349
0.0IleXaa: 0.0 ± 0.0
Lys
5.824LysAla: 5.824 ± 0.565
0.874LysCys: 0.874 ± 0.233
4.999LysAsp: 4.999 ± 0.518
6.164LysGlu: 6.164 ± 0.753
2.427LysPhe: 2.427 ± 0.342
5.048LysGly: 5.048 ± 0.612
1.408LysHis: 1.408 ± 0.289
4.32LysIle: 4.32 ± 0.408
5.776LysLys: 5.776 ± 0.73
6.213LysLeu: 6.213 ± 0.524
2.524LysMet: 2.524 ± 0.407
4.174LysAsn: 4.174 ± 0.433
2.524LysPro: 2.524 ± 0.336
3.106LysGln: 3.106 ± 0.383
3.155LysArg: 3.155 ± 0.349
4.32LysSer: 4.32 ± 0.53
5.339LysThr: 5.339 ± 0.572
6.407LysVal: 6.407 ± 0.651
0.631LysTrp: 0.631 ± 0.191
2.67LysTyr: 2.67 ± 0.539
0.0LysXaa: 0.0 ± 0.0
Leu
6.164LeuAla: 6.164 ± 0.533
0.825LeuCys: 0.825 ± 0.219
5.485LeuAsp: 5.485 ± 0.571
6.65LeuGlu: 6.65 ± 0.556
2.572LeuPhe: 2.572 ± 0.288
4.077LeuGly: 4.077 ± 0.39
1.65LeuHis: 1.65 ± 0.31
3.446LeuIle: 3.446 ± 0.335
6.795LeuLys: 6.795 ± 0.585
5.921LeuLeu: 5.921 ± 0.571
2.67LeuMet: 2.67 ± 0.316
4.029LeuAsn: 4.029 ± 0.439
3.349LeuPro: 3.349 ± 0.363
3.3LeuGln: 3.3 ± 0.452
3.98LeuArg: 3.98 ± 0.402
5.436LeuSer: 5.436 ± 0.611
4.854LeuThr: 4.854 ± 0.489
4.223LeuVal: 4.223 ± 0.429
1.116LeuTrp: 1.116 ± 0.225
3.3LeuTyr: 3.3 ± 0.505
0.0LeuXaa: 0.0 ± 0.0
Met
2.136MetAla: 2.136 ± 0.347
0.34MetCys: 0.34 ± 0.129
1.165MetAsp: 1.165 ± 0.26
1.893MetGlu: 1.893 ± 0.31
1.505MetPhe: 1.505 ± 0.323
1.747MetGly: 1.747 ± 0.262
0.291MetHis: 0.291 ± 0.13
1.796MetIle: 1.796 ± 0.303
2.524MetLys: 2.524 ± 0.376
2.281MetLeu: 2.281 ± 0.402
0.68MetMet: 0.68 ± 0.245
1.602MetAsn: 1.602 ± 0.302
0.777MetPro: 0.777 ± 0.204
1.408MetGln: 1.408 ± 0.334
1.553MetArg: 1.553 ± 0.244
2.136MetSer: 2.136 ± 0.286
1.893MetThr: 1.893 ± 0.298
1.213MetVal: 1.213 ± 0.22
0.243MetTrp: 0.243 ± 0.099
0.777MetTyr: 0.777 ± 0.171
0.0MetXaa: 0.0 ± 0.0
Asn
4.174AsnAla: 4.174 ± 0.459
0.582AsnCys: 0.582 ± 0.186
2.961AsnAsp: 2.961 ± 0.412
2.572AsnGlu: 2.572 ± 0.323
2.572AsnPhe: 2.572 ± 0.321
4.562AsnGly: 4.562 ± 0.549
1.213AsnHis: 1.213 ± 0.325
3.689AsnIle: 3.689 ± 0.547
3.689AsnLys: 3.689 ± 0.523
4.854AsnLeu: 4.854 ± 0.616
1.068AsnMet: 1.068 ± 0.278
2.815AsnAsn: 2.815 ± 0.465
2.136AsnPro: 2.136 ± 0.291
1.65AsnGln: 1.65 ± 0.338
1.941AsnArg: 1.941 ± 0.317
3.3AsnSer: 3.3 ± 0.419
2.815AsnThr: 2.815 ± 0.551
3.689AsnVal: 3.689 ± 0.451
0.68AsnTrp: 0.68 ± 0.132
2.524AsnTyr: 2.524 ± 0.356
0.0AsnXaa: 0.0 ± 0.0
Pro
1.99ProAla: 1.99 ± 0.343
0.437ProCys: 0.437 ± 0.128
2.136ProAsp: 2.136 ± 0.379
2.912ProGlu: 2.912 ± 0.403
1.602ProPhe: 1.602 ± 0.295
0.291ProGly: 0.291 ± 0.149
0.631ProHis: 0.631 ± 0.216
1.262ProIle: 1.262 ± 0.205
2.427ProLys: 2.427 ± 0.41
2.33ProLeu: 2.33 ± 0.319
1.213ProMet: 1.213 ± 0.254
1.553ProAsn: 1.553 ± 0.268
1.019ProPro: 1.019 ± 0.282
1.165ProGln: 1.165 ± 0.213
0.971ProArg: 0.971 ± 0.251
2.524ProSer: 2.524 ± 0.485
2.233ProThr: 2.233 ± 0.31
2.33ProVal: 2.33 ± 0.329
0.146ProTrp: 0.146 ± 0.088
1.359ProTyr: 1.359 ± 0.238
0.0ProXaa: 0.0 ± 0.0
Gln
2.67GlnAla: 2.67 ± 0.392
0.34GlnCys: 0.34 ± 0.121
1.699GlnAsp: 1.699 ± 0.297
2.475GlnGlu: 2.475 ± 0.397
1.699GlnPhe: 1.699 ± 0.263
1.796GlnGly: 1.796 ± 0.342
0.582GlnHis: 0.582 ± 0.175
2.718GlnIle: 2.718 ± 0.346
2.475GlnLys: 2.475 ± 0.317
3.203GlnLeu: 3.203 ± 0.424
1.068GlnMet: 1.068 ± 0.309
1.844GlnAsn: 1.844 ± 0.254
1.31GlnPro: 1.31 ± 0.253
1.359GlnGln: 1.359 ± 0.267
1.699GlnArg: 1.699 ± 0.307
1.893GlnSer: 1.893 ± 0.299
1.893GlnThr: 1.893 ± 0.326
2.427GlnVal: 2.427 ± 0.36
0.485GlnTrp: 0.485 ± 0.148
1.796GlnTyr: 1.796 ± 0.295
0.0GlnXaa: 0.0 ± 0.0
Arg
2.427ArgAla: 2.427 ± 0.318
0.728ArgCys: 0.728 ± 0.176
3.106ArgAsp: 3.106 ± 0.48
1.99ArgGlu: 1.99 ± 0.301
1.408ArgPhe: 1.408 ± 0.325
2.67ArgGly: 2.67 ± 0.451
0.777ArgHis: 0.777 ± 0.2
2.67ArgIle: 2.67 ± 0.276
2.961ArgLys: 2.961 ± 0.343
3.64ArgLeu: 3.64 ± 0.468
1.262ArgMet: 1.262 ± 0.29
2.378ArgAsn: 2.378 ± 0.273
1.068ArgPro: 1.068 ± 0.185
1.553ArgGln: 1.553 ± 0.294
2.039ArgArg: 2.039 ± 0.38
2.039ArgSer: 2.039 ± 0.288
2.475ArgThr: 2.475 ± 0.451
3.106ArgVal: 3.106 ± 0.338
0.68ArgTrp: 0.68 ± 0.25
2.184ArgTyr: 2.184 ± 0.35
0.0ArgXaa: 0.0 ± 0.0
Ser
4.271SerAla: 4.271 ± 0.572
0.582SerCys: 0.582 ± 0.187
4.951SerAsp: 4.951 ± 0.533
4.611SerGlu: 4.611 ± 0.552
2.961SerPhe: 2.961 ± 0.41
3.931SerGly: 3.931 ± 0.554
1.213SerHis: 1.213 ± 0.241
3.446SerIle: 3.446 ± 0.504
4.514SerLys: 4.514 ± 0.466
5.97SerLeu: 5.97 ± 0.547
1.65SerMet: 1.65 ± 0.255
3.203SerAsn: 3.203 ± 0.452
1.747SerPro: 1.747 ± 0.302
2.524SerGln: 2.524 ± 0.42
2.718SerArg: 2.718 ± 0.318
4.417SerSer: 4.417 ± 0.471
3.009SerThr: 3.009 ± 0.498
5.096SerVal: 5.096 ± 0.471
0.971SerTrp: 0.971 ± 0.21
2.864SerTyr: 2.864 ± 0.287
0.0SerXaa: 0.0 ± 0.0
Thr
4.32ThrAla: 4.32 ± 0.562
0.631ThrCys: 0.631 ± 0.163
3.737ThrAsp: 3.737 ± 0.423
3.009ThrGlu: 3.009 ± 0.404
2.864ThrPhe: 2.864 ± 0.376
5.436ThrGly: 5.436 ± 0.563
1.456ThrHis: 1.456 ± 0.343
3.931ThrIle: 3.931 ± 0.569
4.805ThrLys: 4.805 ± 0.544
4.66ThrLeu: 4.66 ± 0.492
0.68ThrMet: 0.68 ± 0.161
2.378ThrAsn: 2.378 ± 0.484
2.281ThrPro: 2.281 ± 0.33
2.718ThrGln: 2.718 ± 0.455
2.572ThrArg: 2.572 ± 0.296
3.592ThrSer: 3.592 ± 0.485
3.689ThrThr: 3.689 ± 0.684
4.951ThrVal: 4.951 ± 0.572
0.631ThrTrp: 0.631 ± 0.206
2.912ThrTyr: 2.912 ± 0.397
0.0ThrXaa: 0.0 ± 0.0
Val
6.067ValAla: 6.067 ± 0.504
1.019ValCys: 1.019 ± 0.189
4.757ValAsp: 4.757 ± 0.488
4.077ValGlu: 4.077 ± 0.554
2.572ValPhe: 2.572 ± 0.353
4.951ValGly: 4.951 ± 0.515
1.262ValHis: 1.262 ± 0.284
4.32ValIle: 4.32 ± 0.499
6.067ValLys: 6.067 ± 0.477
4.174ValLeu: 4.174 ± 0.407
2.184ValMet: 2.184 ± 0.368
4.271ValAsn: 4.271 ± 0.423
2.475ValPro: 2.475 ± 0.387
2.184ValGln: 2.184 ± 0.36
2.815ValArg: 2.815 ± 0.417
5.048ValSer: 5.048 ± 0.42
4.66ValThr: 4.66 ± 0.571
4.271ValVal: 4.271 ± 0.502
0.68ValTrp: 0.68 ± 0.201
2.67ValTyr: 2.67 ± 0.352
0.0ValXaa: 0.0 ± 0.0
Trp
0.388TrpAla: 0.388 ± 0.178
0.194TrpCys: 0.194 ± 0.085
1.019TrpAsp: 1.019 ± 0.195
1.019TrpGlu: 1.019 ± 0.186
0.437TrpPhe: 0.437 ± 0.125
0.728TrpGly: 0.728 ± 0.163
0.049TrpHis: 0.049 ± 0.046
0.68TrpIle: 0.68 ± 0.209
0.874TrpLys: 0.874 ± 0.193
1.165TrpLeu: 1.165 ± 0.271
0.34TrpMet: 0.34 ± 0.14
0.825TrpAsn: 0.825 ± 0.206
0.243TrpPro: 0.243 ± 0.115
0.437TrpGln: 0.437 ± 0.123
0.485TrpArg: 0.485 ± 0.186
0.874TrpSer: 0.874 ± 0.219
0.68TrpThr: 0.68 ± 0.182
0.777TrpVal: 0.777 ± 0.169
0.194TrpTrp: 0.194 ± 0.093
0.777TrpTyr: 0.777 ± 0.16
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.912TyrAla: 2.912 ± 0.418
0.874TyrCys: 0.874 ± 0.199
2.33TyrAsp: 2.33 ± 0.331
2.815TyrGlu: 2.815 ± 0.333
1.553TyrPhe: 1.553 ± 0.249
3.009TyrGly: 3.009 ± 0.426
0.777TyrHis: 0.777 ± 0.211
2.524TyrIle: 2.524 ± 0.323
3.446TyrLys: 3.446 ± 0.368
3.64TyrLeu: 3.64 ± 0.444
1.068TyrMet: 1.068 ± 0.231
2.524TyrAsn: 2.524 ± 0.378
1.602TyrPro: 1.602 ± 0.291
1.602TyrGln: 1.602 ± 0.331
1.456TyrArg: 1.456 ± 0.262
3.009TyrSer: 3.009 ± 0.345
3.495TyrThr: 3.495 ± 0.408
2.427TyrVal: 2.427 ± 0.303
0.388TyrTrp: 0.388 ± 0.15
1.699TyrTyr: 1.699 ± 0.311
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (20604 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski