Metarhizium rileyi (strain RCEF 4871) (Nomuraea rileyi)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium; Metarhizium rileyi

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8763 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A167I236|A0A167I236_METRR Bicarbonate transporter eukaryotic OS=Metarhizium rileyi (strain RCEF 4871) OX=1081105 GN=NOR_01833 PE=4 SV=1
MM1 pKa = 7.19EE2 pKa = 4.09QQILMGFYY10 pKa = 10.87DD11 pKa = 4.8VISLNHH17 pKa = 5.78GMISSLDD24 pKa = 3.45NALFDD29 pKa = 4.51HH30 pKa = 6.55VLLGMNDD37 pKa = 3.5FKK39 pKa = 11.71LEE41 pKa = 3.97VLTWDD46 pKa = 3.37TLTWDD51 pKa = 3.47TCC53 pKa = 4.71

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A167DBC2|A0A167DBC2_METRR Amidohydrolase family protein OS=Metarhizium rileyi (strain RCEF 4871) OX=1081105 GN=NOR_05015 PE=4 SV=1
MM1 pKa = 7.58PFGRR5 pKa = 11.84RR6 pKa = 11.84AHH8 pKa = 6.1HH9 pKa = 5.7TTTTTRR15 pKa = 11.84APRR18 pKa = 11.84RR19 pKa = 11.84GLFSRR24 pKa = 11.84RR25 pKa = 11.84VHH27 pKa = 5.18HH28 pKa = 6.3QKK30 pKa = 10.48RR31 pKa = 11.84KK32 pKa = 8.13PTMKK36 pKa = 10.46DD37 pKa = 2.98KK38 pKa = 11.5VSGALMKK45 pKa = 10.77LRR47 pKa = 11.84GSLTRR52 pKa = 11.84RR53 pKa = 11.84PGLKK57 pKa = 9.99AAGARR62 pKa = 11.84RR63 pKa = 11.84MHH65 pKa = 5.96GTDD68 pKa = 2.82GLGGHH73 pKa = 5.93KK74 pKa = 9.83HH75 pKa = 4.65GHH77 pKa = 4.72VFF79 pKa = 4.0

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8763

0

8763

4465319

49

10347

509.6

56.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.732 ± 0.019

1.255 ± 0.009

5.878 ± 0.02

6.039 ± 0.027

3.627 ± 0.015

6.913 ± 0.024

2.457 ± 0.012

4.675 ± 0.015

4.882 ± 0.026

8.856 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.262 ± 0.01

3.599 ± 0.012

5.917 ± 0.024

4.088 ± 0.017

6.369 ± 0.022

8.327 ± 0.026

5.874 ± 0.017

6.213 ± 0.02

1.41 ± 0.009

2.627 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski