Aspergillus brasiliensis (strain CBS 101740 / IMI 381727 / IBT 21946)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus brasiliensis

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12982 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L9URS8|A0A1L9URS8_ASPBC Uncharacterized protein OS=Aspergillus brasiliensis (strain CBS 101740 / IMI 381727 / IBT 21946) OX=767769 GN=ASPBRDRAFT_193058 PE=4 SV=1
MM1 pKa = 7.18VSSSVIVLTLWAALVSASPVADD23 pKa = 3.73PLVTPAPKK31 pKa = 10.7LEE33 pKa = 4.33DD34 pKa = 3.46LEE36 pKa = 4.92KK37 pKa = 10.62RR38 pKa = 11.84ATSCTFSGSKK48 pKa = 9.97GASSASKK55 pKa = 10.7SKK57 pKa = 9.53TSCSTIVLSDD67 pKa = 3.44VAVPSGTTLDD77 pKa = 3.9LTDD80 pKa = 5.4LNDD83 pKa = 3.69GTHH86 pKa = 6.48VIFEE90 pKa = 4.49GEE92 pKa = 4.06TTFGYY97 pKa = 10.42KK98 pKa = 9.1EE99 pKa = 3.82WSGPLVSVSGTDD111 pKa = 3.23ITVTGADD118 pKa = 3.38GAYY121 pKa = 10.71LNGDD125 pKa = 3.92GSRR128 pKa = 11.84WWDD131 pKa = 3.38DD132 pKa = 3.26EE133 pKa = 4.13GSNGGKK139 pKa = 8.24TKK141 pKa = 10.59PKK143 pKa = 10.12FFYY146 pKa = 10.74AHH148 pKa = 7.7DD149 pKa = 4.24LTSSTISGIYY159 pKa = 8.66IQNSPVQVFSIDD171 pKa = 3.1GSTDD175 pKa = 3.07LTLEE179 pKa = 5.37DD180 pKa = 3.42ITIDD184 pKa = 3.96NSDD187 pKa = 3.9GDD189 pKa = 4.52DD190 pKa = 3.38EE191 pKa = 5.12GAANTDD197 pKa = 3.17GFDD200 pKa = 3.88IGDD203 pKa = 3.68STYY206 pKa = 9.73ITITGANVYY215 pKa = 10.14NQDD218 pKa = 3.1DD219 pKa = 4.24CVAVNSGEE227 pKa = 4.07NIYY230 pKa = 10.7FSGGVCSGGHH240 pKa = 5.7GLSIGSVGGRR250 pKa = 11.84SDD252 pKa = 3.45NTVKK256 pKa = 10.81NVTFYY261 pKa = 11.3NSEE264 pKa = 3.84IKK266 pKa = 10.65NSQNGVRR273 pKa = 11.84IKK275 pKa = 9.88TIYY278 pKa = 10.53GDD280 pKa = 3.51TGSVSEE286 pKa = 4.22VTYY289 pKa = 11.1KK290 pKa = 10.76EE291 pKa = 3.78ITLSEE296 pKa = 3.79ITKK299 pKa = 10.57YY300 pKa = 10.95GIIVQQNYY308 pKa = 10.46DD309 pKa = 3.91DD310 pKa = 4.27TSEE313 pKa = 4.37SPTDD317 pKa = 4.3GITIEE322 pKa = 5.64DD323 pKa = 3.85FVLDD327 pKa = 3.86NVQGSVEE334 pKa = 4.1SSGTNIYY341 pKa = 9.42IVCGSDD347 pKa = 4.0SCTDD351 pKa = 3.47WTWTDD356 pKa = 2.93VDD358 pKa = 3.88VTGGKK363 pKa = 8.27TSSSCEE369 pKa = 3.93NVPDD373 pKa = 6.1DD374 pKa = 4.49ISCC377 pKa = 3.93

Molecular weight:
39.56 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L9UCD6|A0A1L9UCD6_ASPBC Component of oligomeric Golgi complex 2 OS=Aspergillus brasiliensis (strain CBS 101740 / IMI 381727 / IBT 21946) OX=767769 GN=ASPBRDRAFT_57044 PE=3 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.54LAKK15 pKa = 9.74AQRR18 pKa = 11.84QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.78RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.25WRR45 pKa = 11.84KK46 pKa = 9.64SRR48 pKa = 11.84LGVV51 pKa = 3.24

Molecular weight:
6.25 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12981

1

12982

6024818

49

7067

464.1

51.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.422 ± 0.018

1.345 ± 0.008

5.59 ± 0.013

6.04 ± 0.019

3.749 ± 0.013

6.782 ± 0.02

2.49 ± 0.009

4.994 ± 0.016

4.357 ± 0.017

9.205 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.184 ± 0.008

3.55 ± 0.011

6.024 ± 0.02

4.017 ± 0.016

6.137 ± 0.018

8.316 ± 0.027

6.061 ± 0.017

6.262 ± 0.016

1.526 ± 0.008

2.951 ± 0.011

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski