Amino acid dipepetide frequency for Microbacterium phage Nucci

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.244AlaAla: 10.244 ± 1.21
0.938AlaCys: 0.938 ± 0.262
6.569AlaAsp: 6.569 ± 0.84
5.943AlaGlu: 5.943 ± 0.639
2.424AlaPhe: 2.424 ± 0.375
8.211AlaGly: 8.211 ± 0.997
1.408AlaHis: 1.408 ± 0.319
6.021AlaIle: 6.021 ± 0.7
4.066AlaLys: 4.066 ± 0.659
10.166AlaLeu: 10.166 ± 1.201
3.05AlaMet: 3.05 ± 0.402
3.597AlaAsn: 3.597 ± 0.501
4.692AlaPro: 4.692 ± 0.65
3.754AlaGln: 3.754 ± 0.499
6.256AlaArg: 6.256 ± 0.761
5.317AlaSer: 5.317 ± 0.574
6.256AlaThr: 6.256 ± 0.656
7.351AlaVal: 7.351 ± 0.704
1.564AlaTrp: 1.564 ± 0.372
2.659AlaTyr: 2.659 ± 0.465
0.0AlaXaa: 0.0 ± 0.0
Cys
0.938CysAla: 0.938 ± 0.271
0.078CysCys: 0.078 ± 0.074
0.547CysAsp: 0.547 ± 0.25
0.313CysGlu: 0.313 ± 0.153
0.078CysPhe: 0.078 ± 0.071
0.626CysGly: 0.626 ± 0.229
0.078CysHis: 0.078 ± 0.081
0.078CysIle: 0.078 ± 0.083
0.469CysLys: 0.469 ± 0.259
0.156CysLeu: 0.156 ± 0.094
0.078CysMet: 0.078 ± 0.085
0.078CysAsn: 0.078 ± 0.073
0.547CysPro: 0.547 ± 0.178
0.0CysGln: 0.0 ± 0.0
0.704CysArg: 0.704 ± 0.208
0.626CysSer: 0.626 ± 0.21
0.391CysThr: 0.391 ± 0.171
0.391CysVal: 0.391 ± 0.14
0.313CysTrp: 0.313 ± 0.134
0.235CysTyr: 0.235 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
5.708AspAla: 5.708 ± 0.749
0.626AspCys: 0.626 ± 0.236
5.552AspAsp: 5.552 ± 0.783
5.317AspGlu: 5.317 ± 1.011
1.955AspPhe: 1.955 ± 0.369
4.223AspGly: 4.223 ± 0.601
1.408AspHis: 1.408 ± 0.293
3.675AspIle: 3.675 ± 0.434
3.05AspLys: 3.05 ± 0.475
6.021AspLeu: 6.021 ± 0.725
1.642AspMet: 1.642 ± 0.328
1.408AspAsn: 1.408 ± 0.273
5.005AspPro: 5.005 ± 0.558
2.972AspGln: 2.972 ± 0.415
3.128AspArg: 3.128 ± 0.457
2.972AspSer: 2.972 ± 0.557
4.066AspThr: 4.066 ± 0.579
4.692AspVal: 4.692 ± 0.534
1.642AspTrp: 1.642 ± 0.372
1.564AspTyr: 1.564 ± 0.331
0.0AspXaa: 0.0 ± 0.0
Glu
6.803GluAla: 6.803 ± 0.77
0.469GluCys: 0.469 ± 0.185
5.005GluAsp: 5.005 ± 0.863
5.005GluGlu: 5.005 ± 1.262
1.877GluPhe: 1.877 ± 0.411
4.614GluGly: 4.614 ± 0.577
0.782GluHis: 0.782 ± 0.23
1.564GluIle: 1.564 ± 0.328
2.033GluLys: 2.033 ± 0.331
4.926GluLeu: 4.926 ± 0.674
1.564GluMet: 1.564 ± 0.406
1.877GluAsn: 1.877 ± 0.411
1.955GluPro: 1.955 ± 0.386
2.346GluGln: 2.346 ± 0.375
3.91GluArg: 3.91 ± 0.573
2.581GluSer: 2.581 ± 0.469
4.301GluThr: 4.301 ± 0.567
4.614GluVal: 4.614 ± 0.709
1.642GluTrp: 1.642 ± 0.369
1.72GluTyr: 1.72 ± 0.338
0.0GluXaa: 0.0 ± 0.0
Phe
2.033PheAla: 2.033 ± 0.313
0.313PheCys: 0.313 ± 0.141
1.72PheAsp: 1.72 ± 0.379
1.799PheGlu: 1.799 ± 0.354
0.704PhePhe: 0.704 ± 0.22
3.05PheGly: 3.05 ± 0.46
0.626PheHis: 0.626 ± 0.188
1.173PheIle: 1.173 ± 0.363
1.799PheLys: 1.799 ± 0.313
2.346PheLeu: 2.346 ± 0.359
0.547PheMet: 0.547 ± 0.232
1.095PheAsn: 1.095 ± 0.233
1.486PhePro: 1.486 ± 0.274
0.938PheGln: 0.938 ± 0.261
2.19PheArg: 2.19 ± 0.444
2.659PheSer: 2.659 ± 0.303
2.346PheThr: 2.346 ± 0.491
2.424PheVal: 2.424 ± 0.48
0.626PheTrp: 0.626 ± 0.337
0.626PheTyr: 0.626 ± 0.231
0.0PheXaa: 0.0 ± 0.0
Gly
7.194GlyAla: 7.194 ± 0.897
0.469GlyCys: 0.469 ± 0.194
4.066GlyAsp: 4.066 ± 0.43
3.206GlyGlu: 3.206 ± 0.425
2.346GlyPhe: 2.346 ± 0.398
5.552GlyGly: 5.552 ± 0.76
1.72GlyHis: 1.72 ± 0.433
4.692GlyIle: 4.692 ± 1.026
4.614GlyLys: 4.614 ± 0.597
5.63GlyLeu: 5.63 ± 0.838
2.581GlyMet: 2.581 ± 0.387
2.893GlyAsn: 2.893 ± 0.495
3.05GlyPro: 3.05 ± 0.389
3.675GlyGln: 3.675 ± 0.683
5.63GlyArg: 5.63 ± 0.676
4.536GlySer: 4.536 ± 0.597
7.194GlyThr: 7.194 ± 0.799
6.49GlyVal: 6.49 ± 0.733
1.486GlyTrp: 1.486 ± 0.372
2.737GlyTyr: 2.737 ± 0.536
0.0GlyXaa: 0.0 ± 0.0
His
1.486HisAla: 1.486 ± 0.375
0.235HisCys: 0.235 ± 0.123
0.938HisAsp: 0.938 ± 0.262
0.938HisGlu: 0.938 ± 0.234
0.86HisPhe: 0.86 ± 0.258
1.799HisGly: 1.799 ± 0.379
0.391HisHis: 0.391 ± 0.199
1.251HisIle: 1.251 ± 0.355
1.017HisLys: 1.017 ± 0.384
1.408HisLeu: 1.408 ± 0.388
0.391HisMet: 0.391 ± 0.171
0.547HisAsn: 0.547 ± 0.214
0.626HisPro: 0.626 ± 0.197
0.782HisGln: 0.782 ± 0.292
0.86HisArg: 0.86 ± 0.284
0.704HisSer: 0.704 ± 0.245
1.173HisThr: 1.173 ± 0.348
1.408HisVal: 1.408 ± 0.334
0.313HisTrp: 0.313 ± 0.121
0.782HisTyr: 0.782 ± 0.219
0.0HisXaa: 0.0 ± 0.0
Ile
4.848IleAla: 4.848 ± 0.435
0.313IleCys: 0.313 ± 0.15
3.675IleAsp: 3.675 ± 0.427
3.363IleGlu: 3.363 ± 0.697
0.626IlePhe: 0.626 ± 0.282
4.614IleGly: 4.614 ± 0.943
0.86IleHis: 0.86 ± 0.262
3.284IleIle: 3.284 ± 0.975
2.268IleLys: 2.268 ± 0.504
3.284IleLeu: 3.284 ± 0.488
1.017IleMet: 1.017 ± 0.284
2.111IleAsn: 2.111 ± 0.374
2.268IlePro: 2.268 ± 0.602
2.659IleGln: 2.659 ± 0.425
2.893IleArg: 2.893 ± 0.544
2.972IleSer: 2.972 ± 0.581
3.519IleThr: 3.519 ± 0.659
2.502IleVal: 2.502 ± 0.469
0.938IleTrp: 0.938 ± 0.255
1.251IleTyr: 1.251 ± 0.324
0.0IleXaa: 0.0 ± 0.0
Lys
6.334LysAla: 6.334 ± 0.858
0.235LysCys: 0.235 ± 0.12
2.581LysAsp: 2.581 ± 0.54
2.972LysGlu: 2.972 ± 0.738
0.86LysPhe: 0.86 ± 0.215
3.675LysGly: 3.675 ± 0.514
0.704LysHis: 0.704 ± 0.229
1.486LysIle: 1.486 ± 0.295
2.815LysLys: 2.815 ± 0.518
4.223LysLeu: 4.223 ± 0.594
1.486LysMet: 1.486 ± 0.334
1.251LysAsn: 1.251 ± 0.341
3.754LysPro: 3.754 ± 0.624
0.938LysGln: 0.938 ± 0.228
2.346LysArg: 2.346 ± 0.524
2.346LysSer: 2.346 ± 0.44
2.737LysThr: 2.737 ± 0.493
3.988LysVal: 3.988 ± 0.469
1.251LysTrp: 1.251 ± 0.448
0.782LysTyr: 0.782 ± 0.221
0.0LysXaa: 0.0 ± 0.0
Leu
9.149LeuAla: 9.149 ± 0.955
0.626LeuCys: 0.626 ± 0.235
5.63LeuAsp: 5.63 ± 0.613
4.457LeuGlu: 4.457 ± 0.538
2.581LeuPhe: 2.581 ± 0.395
6.099LeuGly: 6.099 ± 0.868
1.486LeuHis: 1.486 ± 0.359
4.145LeuIle: 4.145 ± 0.754
3.832LeuLys: 3.832 ± 0.625
7.429LeuLeu: 7.429 ± 0.905
1.72LeuMet: 1.72 ± 0.353
2.972LeuAsn: 2.972 ± 0.389
4.379LeuPro: 4.379 ± 0.785
3.206LeuGln: 3.206 ± 0.367
5.239LeuArg: 5.239 ± 0.665
4.379LeuSer: 4.379 ± 0.796
6.569LeuThr: 6.569 ± 0.847
6.803LeuVal: 6.803 ± 0.745
0.86LeuTrp: 0.86 ± 0.239
1.955LeuTyr: 1.955 ± 0.349
0.0LeuXaa: 0.0 ± 0.0
Met
2.815MetAla: 2.815 ± 0.373
0.235MetCys: 0.235 ± 0.157
2.033MetAsp: 2.033 ± 0.368
1.564MetGlu: 1.564 ± 0.314
0.86MetPhe: 0.86 ± 0.25
2.033MetGly: 2.033 ± 0.514
0.469MetHis: 0.469 ± 0.201
0.782MetIle: 0.782 ± 0.227
0.86MetLys: 0.86 ± 0.25
2.111MetLeu: 2.111 ± 0.358
0.235MetMet: 0.235 ± 0.125
1.408MetAsn: 1.408 ± 0.333
1.72MetPro: 1.72 ± 0.404
0.938MetGln: 0.938 ± 0.222
1.642MetArg: 1.642 ± 0.321
2.268MetSer: 2.268 ± 0.447
1.408MetThr: 1.408 ± 0.291
1.799MetVal: 1.799 ± 0.315
0.391MetTrp: 0.391 ± 0.169
0.469MetTyr: 0.469 ± 0.193
0.0MetXaa: 0.0 ± 0.0
Asn
3.128AsnAla: 3.128 ± 0.51
0.078AsnCys: 0.078 ± 0.082
1.799AsnAsp: 1.799 ± 0.364
1.799AsnGlu: 1.799 ± 0.477
0.86AsnPhe: 0.86 ± 0.209
2.737AsnGly: 2.737 ± 0.481
1.017AsnHis: 1.017 ± 0.274
2.033AsnIle: 2.033 ± 0.405
2.111AsnLys: 2.111 ± 0.387
2.893AsnLeu: 2.893 ± 0.467
0.86AsnMet: 0.86 ± 0.193
1.017AsnAsn: 1.017 ± 0.273
2.033AsnPro: 2.033 ± 0.319
1.017AsnGln: 1.017 ± 0.301
1.72AsnArg: 1.72 ± 0.387
2.033AsnSer: 2.033 ± 0.401
2.424AsnThr: 2.424 ± 0.366
2.033AsnVal: 2.033 ± 0.429
0.938AsnTrp: 0.938 ± 0.234
1.173AsnTyr: 1.173 ± 0.291
0.0AsnXaa: 0.0 ± 0.0
Pro
5.396ProAla: 5.396 ± 0.863
0.078ProCys: 0.078 ± 0.083
3.91ProAsp: 3.91 ± 0.465
2.581ProGlu: 2.581 ± 0.404
1.799ProPhe: 1.799 ± 0.372
4.301ProGly: 4.301 ± 0.644
0.626ProHis: 0.626 ± 0.228
1.408ProIle: 1.408 ± 0.319
3.128ProLys: 3.128 ± 0.36
3.519ProLeu: 3.519 ± 0.352
0.782ProMet: 0.782 ± 0.243
1.642ProAsn: 1.642 ± 0.415
1.173ProPro: 1.173 ± 0.324
1.877ProGln: 1.877 ± 0.519
2.424ProArg: 2.424 ± 0.457
3.675ProSer: 3.675 ± 0.497
3.441ProThr: 3.441 ± 0.542
4.457ProVal: 4.457 ± 0.575
1.017ProTrp: 1.017 ± 0.27
1.486ProTyr: 1.486 ± 0.266
0.0ProXaa: 0.0 ± 0.0
Gln
4.145GlnAla: 4.145 ± 0.527
0.156GlnCys: 0.156 ± 0.105
2.581GlnAsp: 2.581 ± 0.42
2.815GlnGlu: 2.815 ± 0.506
1.017GlnPhe: 1.017 ± 0.255
2.424GlnGly: 2.424 ± 0.453
0.86GlnHis: 0.86 ± 0.268
2.659GlnIle: 2.659 ± 0.626
1.564GlnLys: 1.564 ± 0.322
3.284GlnLeu: 3.284 ± 0.472
0.782GlnMet: 0.782 ± 0.24
1.095GlnAsn: 1.095 ± 0.275
1.877GlnPro: 1.877 ± 0.334
2.502GlnGln: 2.502 ± 0.527
2.581GlnArg: 2.581 ± 0.344
1.955GlnSer: 1.955 ± 0.271
2.815GlnThr: 2.815 ± 0.36
2.972GlnVal: 2.972 ± 0.442
0.704GlnTrp: 0.704 ± 0.245
0.938GlnTyr: 0.938 ± 0.21
0.0GlnXaa: 0.0 ± 0.0
Arg
5.708ArgAla: 5.708 ± 0.732
0.391ArgCys: 0.391 ± 0.153
4.066ArgAsp: 4.066 ± 0.489
3.441ArgGlu: 3.441 ± 0.53
2.111ArgPhe: 2.111 ± 0.372
3.91ArgGly: 3.91 ± 0.548
0.938ArgHis: 0.938 ± 0.28
2.737ArgIle: 2.737 ± 0.499
3.128ArgLys: 3.128 ± 0.57
5.161ArgLeu: 5.161 ± 0.705
2.111ArgMet: 2.111 ± 0.343
2.033ArgAsn: 2.033 ± 0.349
2.424ArgPro: 2.424 ± 0.429
1.799ArgGln: 1.799 ± 0.382
3.441ArgArg: 3.441 ± 0.706
2.893ArgSer: 2.893 ± 0.411
3.363ArgThr: 3.363 ± 0.576
5.787ArgVal: 5.787 ± 0.848
1.251ArgTrp: 1.251 ± 0.317
2.111ArgTyr: 2.111 ± 0.407
0.0ArgXaa: 0.0 ± 0.0
Ser
5.943SerAla: 5.943 ± 0.808
0.235SerCys: 0.235 ± 0.113
3.284SerAsp: 3.284 ± 0.521
2.972SerGlu: 2.972 ± 0.478
2.893SerPhe: 2.893 ± 0.501
4.77SerGly: 4.77 ± 0.616
1.173SerHis: 1.173 ± 0.365
2.737SerIle: 2.737 ± 0.468
2.581SerLys: 2.581 ± 0.434
4.614SerLeu: 4.614 ± 0.662
1.564SerMet: 1.564 ± 0.303
1.799SerAsn: 1.799 ± 0.334
2.111SerPro: 2.111 ± 0.472
2.346SerGln: 2.346 ± 0.396
3.05SerArg: 3.05 ± 0.553
3.91SerSer: 3.91 ± 0.466
4.066SerThr: 4.066 ± 0.502
4.301SerVal: 4.301 ± 0.599
0.938SerTrp: 0.938 ± 0.24
1.642SerTyr: 1.642 ± 0.324
0.0SerXaa: 0.0 ± 0.0
Thr
5.943ThrAla: 5.943 ± 0.758
0.235ThrCys: 0.235 ± 0.121
3.988ThrAsp: 3.988 ± 0.732
3.206ThrGlu: 3.206 ± 0.443
2.268ThrPhe: 2.268 ± 0.394
5.474ThrGly: 5.474 ± 0.526
1.173ThrHis: 1.173 ± 0.361
3.363ThrIle: 3.363 ± 0.402
2.893ThrLys: 2.893 ± 0.536
5.787ThrLeu: 5.787 ± 0.598
1.564ThrMet: 1.564 ± 0.294
2.268ThrAsn: 2.268 ± 0.421
3.988ThrPro: 3.988 ± 0.456
3.05ThrGln: 3.05 ± 0.415
4.145ThrArg: 4.145 ± 0.497
4.379ThrSer: 4.379 ± 0.629
5.161ThrThr: 5.161 ± 0.786
5.943ThrVal: 5.943 ± 0.675
1.72ThrTrp: 1.72 ± 0.439
1.955ThrTyr: 1.955 ± 0.407
0.0ThrXaa: 0.0 ± 0.0
Val
8.915ValAla: 8.915 ± 0.843
0.313ValCys: 0.313 ± 0.182
5.317ValAsp: 5.317 ± 0.535
4.926ValGlu: 4.926 ± 0.735
2.346ValPhe: 2.346 ± 0.432
6.647ValGly: 6.647 ± 1.031
1.329ValHis: 1.329 ± 0.327
4.223ValIle: 4.223 ± 0.578
2.19ValLys: 2.19 ± 0.468
6.49ValLeu: 6.49 ± 0.647
2.737ValMet: 2.737 ± 0.615
2.815ValAsn: 2.815 ± 0.369
3.206ValPro: 3.206 ± 0.476
3.441ValGln: 3.441 ± 0.477
4.066ValArg: 4.066 ± 0.545
3.832ValSer: 3.832 ± 0.503
4.614ValThr: 4.614 ± 0.591
5.474ValVal: 5.474 ± 0.561
1.72ValTrp: 1.72 ± 0.558
2.346ValTyr: 2.346 ± 0.385
0.0ValXaa: 0.0 ± 0.0
Trp
1.251TrpAla: 1.251 ± 0.321
0.0TrpCys: 0.0 ± 0.0
1.642TrpAsp: 1.642 ± 0.329
1.251TrpGlu: 1.251 ± 0.335
0.86TrpPhe: 0.86 ± 0.323
2.19TrpGly: 2.19 ± 0.541
0.547TrpHis: 0.547 ± 0.168
0.938TrpIle: 0.938 ± 0.259
1.095TrpLys: 1.095 ± 0.325
2.268TrpLeu: 2.268 ± 0.476
0.469TrpMet: 0.469 ± 0.155
0.704TrpAsn: 0.704 ± 0.231
0.782TrpPro: 0.782 ± 0.226
0.547TrpGln: 0.547 ± 0.241
0.938TrpArg: 0.938 ± 0.24
1.173TrpSer: 1.173 ± 0.266
1.329TrpThr: 1.329 ± 0.308
1.329TrpVal: 1.329 ± 0.286
0.547TrpTrp: 0.547 ± 0.276
1.095TrpTyr: 1.095 ± 0.279
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.502TyrAla: 2.502 ± 0.441
0.704TyrCys: 0.704 ± 0.182
1.72TyrAsp: 1.72 ± 0.31
1.72TyrGlu: 1.72 ± 0.34
1.173TyrPhe: 1.173 ± 0.252
2.815TyrGly: 2.815 ± 0.453
0.391TyrHis: 0.391 ± 0.151
1.017TyrIle: 1.017 ± 0.283
1.251TyrLys: 1.251 ± 0.357
1.799TyrLeu: 1.799 ± 0.382
0.782TyrMet: 0.782 ± 0.237
1.095TyrAsn: 1.095 ± 0.273
1.642TyrPro: 1.642 ± 0.346
0.938TyrGln: 0.938 ± 0.221
1.642TyrArg: 1.642 ± 0.37
1.72TyrSer: 1.72 ± 0.404
1.329TyrThr: 1.329 ± 0.295
2.19TyrVal: 2.19 ± 0.409
1.095TyrTrp: 1.095 ± 0.416
1.017TyrTyr: 1.017 ± 0.348
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (12789 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski