Amino acid dipepetide frequency for Arcanobacterium sp. S3PF19

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.219AlaAla: 15.219 ± 0.417
1.079AlaCys: 1.079 ± 0.062
6.982AlaAsp: 6.982 ± 0.149
7.678AlaGlu: 7.678 ± 0.2
3.265AlaPhe: 3.265 ± 0.117
10.809AlaGly: 10.809 ± 0.221
2.292AlaHis: 2.292 ± 0.082
4.377AlaIle: 4.377 ± 0.133
5.21AlaLys: 5.21 ± 0.137
11.207AlaLeu: 11.207 ± 0.211
2.229AlaMet: 2.229 ± 0.09
2.719AlaAsn: 2.719 ± 0.108
4.383AlaPro: 4.383 ± 0.143
4.774AlaGln: 4.774 ± 0.16
7.58AlaArg: 7.58 ± 0.192
4.762AlaSer: 4.762 ± 0.118
4.066AlaThr: 4.066 ± 0.146
10.089AlaVal: 10.089 ± 0.214
1.099AlaTrp: 1.099 ± 0.053
2.45AlaTyr: 2.45 ± 0.088
0.0AlaXaa: 0.0 ± 0.0
Cys
1.831CysAla: 1.831 ± 0.082
0.143CysCys: 0.143 ± 0.019
0.523CysAsp: 0.523 ± 0.042
0.672CysGlu: 0.672 ± 0.045
0.463CysPhe: 0.463 ± 0.039
1.407CysGly: 1.407 ± 0.064
0.173CysHis: 0.173 ± 0.025
0.415CysIle: 0.415 ± 0.039
0.239CysLys: 0.239 ± 0.028
0.977CysLeu: 0.977 ± 0.059
0.152CysMet: 0.152 ± 0.023
0.194CysAsn: 0.194 ± 0.022
0.562CysPro: 0.562 ± 0.043
0.176CysGln: 0.176 ± 0.022
0.741CysArg: 0.741 ± 0.05
0.529CysSer: 0.529 ± 0.04
0.601CysThr: 0.601 ± 0.045
0.777CysVal: 0.777 ± 0.055
0.087CysTrp: 0.087 ± 0.015
0.218CysTyr: 0.218 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
5.599AspAla: 5.599 ± 0.146
0.583AspCys: 0.583 ± 0.042
2.318AspAsp: 2.318 ± 0.085
3.271AspGlu: 3.271 ± 0.115
2.414AspPhe: 2.414 ± 0.097
4.47AspGly: 4.47 ± 0.127
0.753AspHis: 0.753 ± 0.051
3.068AspIle: 3.068 ± 0.112
1.808AspLys: 1.808 ± 0.103
5.694AspLeu: 5.694 ± 0.141
0.959AspMet: 0.959 ± 0.052
1.312AspAsn: 1.312 ± 0.067
3.182AspPro: 3.182 ± 0.102
0.875AspGln: 0.875 ± 0.053
3.466AspArg: 3.466 ± 0.123
3.316AspSer: 3.316 ± 0.107
3.029AspThr: 3.029 ± 0.076
4.069AspVal: 4.069 ± 0.124
0.648AspTrp: 0.648 ± 0.046
1.482AspTyr: 1.482 ± 0.07
0.0AspXaa: 0.0 ± 0.0
Glu
5.787GluAla: 5.787 ± 0.178
0.472GluCys: 0.472 ± 0.04
3.006GluAsp: 3.006 ± 0.097
4.732GluGlu: 4.732 ± 0.169
1.996GluPhe: 1.996 ± 0.086
3.8GluGly: 3.8 ± 0.128
1.138GluHis: 1.138 ± 0.062
4.484GluIle: 4.484 ± 0.106
4.771GluLys: 4.771 ± 0.143
6.116GluLeu: 6.116 ± 0.183
1.572GluMet: 1.572 ± 0.072
3.155GluAsn: 3.155 ± 0.108
2.091GluPro: 2.091 ± 0.075
2.032GluGln: 2.032 ± 0.084
3.779GluArg: 3.779 ± 0.133
3.056GluSer: 3.056 ± 0.098
3.983GluThr: 3.983 ± 0.122
3.89GluVal: 3.89 ± 0.126
0.589GluTrp: 0.589 ± 0.043
1.805GluTyr: 1.805 ± 0.08
0.0GluXaa: 0.0 ± 0.0
Phe
4.296PheAla: 4.296 ± 0.128
0.502PheCys: 0.502 ± 0.035
2.02PheAsp: 2.02 ± 0.079
1.685PheGlu: 1.685 ± 0.067
1.607PhePhe: 1.607 ± 0.073
3.358PheGly: 3.358 ± 0.108
0.562PheHis: 0.562 ± 0.042
1.628PheIle: 1.628 ± 0.078
1.058PheLys: 1.058 ± 0.069
3.373PheLeu: 3.373 ± 0.116
0.723PheMet: 0.723 ± 0.05
0.98PheAsn: 0.98 ± 0.053
1.906PhePro: 1.906 ± 0.078
0.696PheGln: 0.696 ± 0.044
2.208PheArg: 2.208 ± 0.087
3.164PheSer: 3.164 ± 0.104
2.36PheThr: 2.36 ± 0.086
2.734PheVal: 2.734 ± 0.103
0.424PheTrp: 0.424 ± 0.036
0.959PheTyr: 0.959 ± 0.057
0.0PheXaa: 0.0 ± 0.0
Gly
8.192GlyAla: 8.192 ± 0.203
0.899GlyCys: 0.899 ± 0.055
3.815GlyAsp: 3.815 ± 0.117
5.871GlyGlu: 5.871 ± 0.145
3.23GlyPhe: 3.23 ± 0.112
6.37GlyGly: 6.37 ± 0.154
1.506GlyHis: 1.506 ± 0.072
5.467GlyIle: 5.467 ± 0.131
5.572GlyLys: 5.572 ± 0.184
6.946GlyLeu: 6.946 ± 0.191
2.169GlyMet: 2.169 ± 0.083
2.644GlyAsn: 2.644 ± 0.124
2.611GlyPro: 2.611 ± 0.093
2.519GlyGln: 2.519 ± 0.092
5.452GlyArg: 5.452 ± 0.153
5.007GlySer: 5.007 ± 0.138
5.067GlyThr: 5.067 ± 0.131
6.295GlyVal: 6.295 ± 0.147
1.105GlyTrp: 1.105 ± 0.066
2.578GlyTyr: 2.578 ± 0.086
0.0GlyXaa: 0.0 ± 0.0
His
1.763HisAla: 1.763 ± 0.082
0.275HisCys: 0.275 ± 0.029
0.986HisAsp: 0.986 ± 0.058
0.962HisGlu: 0.962 ± 0.052
0.651HisPhe: 0.651 ± 0.044
1.831HisGly: 1.831 ± 0.078
0.406HisHis: 0.406 ± 0.035
1.049HisIle: 1.049 ± 0.057
0.598HisLys: 0.598 ± 0.042
1.778HisLeu: 1.778 ± 0.077
0.376HisMet: 0.376 ± 0.036
0.606HisAsn: 0.606 ± 0.044
1.327HisPro: 1.327 ± 0.061
0.341HisGln: 0.341 ± 0.035
1.461HisArg: 1.461 ± 0.069
1.243HisSer: 1.243 ± 0.064
1.341HisThr: 1.341 ± 0.066
1.243HisVal: 1.243 ± 0.065
0.248HisTrp: 0.248 ± 0.027
0.627HisTyr: 0.627 ± 0.045
0.0HisXaa: 0.0 ± 0.0
Ile
5.951IleAla: 5.951 ± 0.158
0.774IleCys: 0.774 ± 0.053
2.94IleAsp: 2.94 ± 0.101
2.737IleGlu: 2.737 ± 0.101
2.226IlePhe: 2.226 ± 0.109
4.768IleGly: 4.768 ± 0.136
0.962IleHis: 0.962 ± 0.055
2.761IleIle: 2.761 ± 0.11
1.897IleLys: 1.897 ± 0.082
5.187IleLeu: 5.187 ± 0.146
1.135IleMet: 1.135 ± 0.064
1.652IleAsn: 1.652 ± 0.079
3.149IlePro: 3.149 ± 0.11
1.004IleGln: 1.004 ± 0.062
3.66IleArg: 3.66 ± 0.119
3.929IleSer: 3.929 ± 0.11
3.146IleThr: 3.146 ± 0.092
4.078IleVal: 4.078 ± 0.13
0.547IleTrp: 0.547 ± 0.043
1.341IleTyr: 1.341 ± 0.06
0.0IleXaa: 0.0 ± 0.0
Lys
4.691LysAla: 4.691 ± 0.164
0.311LysCys: 0.311 ± 0.025
2.474LysAsp: 2.474 ± 0.092
3.2LysGlu: 3.2 ± 0.112
1.335LysPhe: 1.335 ± 0.065
2.716LysGly: 2.716 ± 0.11
0.834LysHis: 0.834 ± 0.05
3.499LysIle: 3.499 ± 0.12
2.952LysLys: 2.952 ± 0.138
3.738LysLeu: 3.738 ± 0.108
1.395LysMet: 1.395 ± 0.057
2.271LysAsn: 2.271 ± 0.102
2.112LysPro: 2.112 ± 0.098
1.563LysGln: 1.563 ± 0.086
2.662LysArg: 2.662 ± 0.099
2.608LysSer: 2.608 ± 0.085
3.582LysThr: 3.582 ± 0.107
3.044LysVal: 3.044 ± 0.102
0.621LysTrp: 0.621 ± 0.043
1.455LysTyr: 1.455 ± 0.083
0.0LysXaa: 0.0 ± 0.0
Leu
10.887LeuAla: 10.887 ± 0.194
1.362LeuCys: 1.362 ± 0.067
5.061LeuAsp: 5.061 ± 0.134
5.163LeuGlu: 5.163 ± 0.143
3.295LeuPhe: 3.295 ± 0.1
7.63LeuGly: 7.63 ± 0.203
2.035LeuHis: 2.035 ± 0.09
5.118LeuIle: 5.118 ± 0.148
4.096LeuLys: 4.096 ± 0.124
9.366LeuLeu: 9.366 ± 0.26
1.882LeuMet: 1.882 ± 0.08
2.937LeuAsn: 2.937 ± 0.093
5.279LeuPro: 5.279 ± 0.146
2.677LeuGln: 2.677 ± 0.085
7.768LeuArg: 7.768 ± 0.169
6.411LeuSer: 6.411 ± 0.121
5.775LeuThr: 5.775 ± 0.139
6.259LeuVal: 6.259 ± 0.175
1.019LeuTrp: 1.019 ± 0.066
2.07LeuTyr: 2.07 ± 0.078
0.0LeuXaa: 0.0 ± 0.0
Met
2.175MetAla: 2.175 ± 0.082
0.278MetCys: 0.278 ± 0.03
1.079MetAsp: 1.079 ± 0.053
1.174MetGlu: 1.174 ± 0.062
0.666MetPhe: 0.666 ± 0.047
1.727MetGly: 1.727 ± 0.082
0.4MetHis: 0.4 ± 0.038
1.016MetIle: 1.016 ± 0.057
1.129MetLys: 1.129 ± 0.059
2.187MetLeu: 2.187 ± 0.08
0.511MetMet: 0.511 ± 0.042
0.878MetAsn: 0.878 ± 0.055
1.341MetPro: 1.341 ± 0.069
0.72MetGln: 0.72 ± 0.04
1.664MetArg: 1.664 ± 0.068
1.38MetSer: 1.38 ± 0.064
1.321MetThr: 1.321 ± 0.071
1.356MetVal: 1.356 ± 0.064
0.269MetTrp: 0.269 ± 0.028
0.442MetTyr: 0.442 ± 0.042
0.0MetXaa: 0.0 ± 0.0
Asn
3.4AsnAla: 3.4 ± 0.12
0.278AsnCys: 0.278 ± 0.028
1.38AsnAsp: 1.38 ± 0.07
1.748AsnGlu: 1.748 ± 0.083
1.222AsnPhe: 1.222 ± 0.061
2.796AsnGly: 2.796 ± 0.099
0.472AsnHis: 0.472 ± 0.034
1.802AsnIle: 1.802 ± 0.074
1.061AsnLys: 1.061 ± 0.065
2.898AsnLeu: 2.898 ± 0.087
0.723AsnMet: 0.723 ± 0.044
0.932AsnAsn: 0.932 ± 0.061
2.184AsnPro: 2.184 ± 0.079
0.648AsnGln: 0.648 ± 0.043
2.16AsnArg: 2.16 ± 0.085
1.891AsnSer: 1.891 ± 0.075
2.005AsnThr: 2.005 ± 0.082
2.309AsnVal: 2.309 ± 0.09
0.412AsnTrp: 0.412 ± 0.037
0.896AsnTyr: 0.896 ± 0.053
0.0AsnXaa: 0.0 ± 0.0
Pro
6.161ProAla: 6.161 ± 0.175
0.427ProCys: 0.427 ± 0.037
2.826ProAsp: 2.826 ± 0.103
3.394ProGlu: 3.394 ± 0.111
1.607ProPhe: 1.607 ± 0.06
4.801ProGly: 4.801 ± 0.119
1.049ProHis: 1.049 ± 0.056
2.011ProIle: 2.011 ± 0.081
2.151ProLys: 2.151 ± 0.088
4.069ProLeu: 4.069 ± 0.116
0.744ProMet: 0.744 ± 0.05
1.47ProAsn: 1.47 ± 0.072
1.781ProPro: 1.781 ± 0.083
2.247ProGln: 2.247 ± 0.082
2.68ProArg: 2.68 ± 0.1
2.259ProSer: 2.259 ± 0.081
2.286ProThr: 2.286 ± 0.101
3.914ProVal: 3.914 ± 0.118
0.604ProTrp: 0.604 ± 0.047
1.183ProTyr: 1.183 ± 0.057
0.0ProXaa: 0.0 ± 0.0
Gln
3.403GlnAla: 3.403 ± 0.143
0.299GlnCys: 0.299 ± 0.032
1.33GlnAsp: 1.33 ± 0.07
1.679GlnGlu: 1.679 ± 0.079
0.816GlnPhe: 0.816 ± 0.047
1.939GlnGly: 1.939 ± 0.079
0.493GlnHis: 0.493 ± 0.039
2.432GlnIle: 2.432 ± 0.088
1.784GlnLys: 1.784 ± 0.091
2.823GlnLeu: 2.823 ± 0.089
0.887GlnMet: 0.887 ± 0.055
1.246GlnAsn: 1.246 ± 0.069
1.177GlnPro: 1.177 ± 0.062
1.043GlnGln: 1.043 ± 0.063
2.067GlnArg: 2.067 ± 0.088
1.682GlnSer: 1.682 ± 0.074
1.96GlnThr: 1.96 ± 0.074
2.115GlnVal: 2.115 ± 0.078
0.517GlnTrp: 0.517 ± 0.041
0.726GlnTyr: 0.726 ± 0.055
0.0GlnXaa: 0.0 ± 0.0
Arg
7.14ArgAla: 7.14 ± 0.183
0.654ArgCys: 0.654 ± 0.046
3.089ArgAsp: 3.089 ± 0.108
5.234ArgGlu: 5.234 ± 0.144
2.324ArgPhe: 2.324 ± 0.081
5.172ArgGly: 5.172 ± 0.13
1.518ArgHis: 1.518 ± 0.063
4.431ArgIle: 4.431 ± 0.12
3.555ArgLys: 3.555 ± 0.101
6.325ArgLeu: 6.325 ± 0.154
1.697ArgMet: 1.697 ± 0.065
1.99ArgAsn: 1.99 ± 0.076
2.814ArgPro: 2.814 ± 0.104
2.402ArgGln: 2.402 ± 0.095
5.832ArgArg: 5.832 ± 0.159
3.705ArgSer: 3.705 ± 0.106
3.445ArgThr: 3.445 ± 0.105
4.446ArgVal: 4.446 ± 0.129
0.729ArgTrp: 0.729 ± 0.045
1.957ArgTyr: 1.957 ± 0.088
0.0ArgXaa: 0.0 ± 0.0
Ser
7.801SerAla: 7.801 ± 0.211
0.621SerCys: 0.621 ± 0.04
3.143SerAsp: 3.143 ± 0.101
3.203SerGlu: 3.203 ± 0.105
2.309SerPhe: 2.309 ± 0.079
5.757SerGly: 5.757 ± 0.146
1.117SerHis: 1.117 ± 0.061
2.073SerIle: 2.073 ± 0.088
1.837SerLys: 1.837 ± 0.083
5.922SerLeu: 5.922 ± 0.148
1.255SerMet: 1.255 ± 0.065
1.207SerAsn: 1.207 ± 0.057
2.752SerPro: 2.752 ± 0.095
1.703SerGln: 1.703 ± 0.066
4.114SerArg: 4.114 ± 0.111
3.182SerSer: 3.182 ± 0.109
2.501SerThr: 2.501 ± 0.085
5.306SerVal: 5.306 ± 0.134
0.81SerTrp: 0.81 ± 0.052
1.479SerTyr: 1.479 ± 0.066
0.0SerXaa: 0.0 ± 0.0
Thr
6.546ThrAla: 6.546 ± 0.178
0.448ThrCys: 0.448 ± 0.033
3.63ThrAsp: 3.63 ± 0.11
3.735ThrGlu: 3.735 ± 0.113
1.939ThrPhe: 1.939 ± 0.076
5.166ThrGly: 5.166 ± 0.129
1.27ThrHis: 1.27 ± 0.064
2.187ThrIle: 2.187 ± 0.088
2.121ThrLys: 2.121 ± 0.087
5.429ThrLeu: 5.429 ± 0.133
1.034ThrMet: 1.034 ± 0.049
1.318ThrAsn: 1.318 ± 0.061
3.206ThrPro: 3.206 ± 0.109
1.793ThrGln: 1.793 ± 0.073
2.814ThrArg: 2.814 ± 0.094
2.539ThrSer: 2.539 ± 0.074
2.079ThrThr: 2.079 ± 0.088
6.178ThrVal: 6.178 ± 0.156
0.621ThrTrp: 0.621 ± 0.043
1.312ThrTyr: 1.312 ± 0.061
0.0ThrXaa: 0.0 ± 0.0
Val
7.514ValAla: 7.514 ± 0.163
1.073ValCys: 1.073 ± 0.064
3.633ValAsp: 3.633 ± 0.12
3.782ValGlu: 3.782 ± 0.118
3.337ValPhe: 3.337 ± 0.135
5.745ValGly: 5.745 ± 0.139
1.485ValHis: 1.485 ± 0.07
4.156ValIle: 4.156 ± 0.13
3.779ValLys: 3.779 ± 0.118
8.04ValLeu: 8.04 ± 0.217
1.56ValMet: 1.56 ± 0.078
2.504ValAsn: 2.504 ± 0.088
4.093ValPro: 4.093 ± 0.117
2.112ValGln: 2.112 ± 0.081
5.264ValArg: 5.264 ± 0.123
5.399ValSer: 5.399 ± 0.134
4.717ValThr: 4.717 ± 0.136
5.291ValVal: 5.291 ± 0.159
0.851ValTrp: 0.851 ± 0.048
1.769ValTyr: 1.769 ± 0.071
0.0ValXaa: 0.0 ± 0.0
Trp
1.049TrpAla: 1.049 ± 0.053
0.099TrpCys: 0.099 ± 0.02
0.693TrpAsp: 0.693 ± 0.046
0.78TrpGlu: 0.78 ± 0.052
0.43TrpPhe: 0.43 ± 0.038
0.786TrpGly: 0.786 ± 0.049
0.257TrpHis: 0.257 ± 0.028
0.792TrpIle: 0.792 ± 0.044
0.705TrpLys: 0.705 ± 0.052
1.093TrpLeu: 1.093 ± 0.063
0.281TrpMet: 0.281 ± 0.032
0.523TrpAsn: 0.523 ± 0.043
0.344TrpPro: 0.344 ± 0.035
0.502TrpGln: 0.502 ± 0.038
0.75TrpArg: 0.75 ± 0.055
0.615TrpSer: 0.615 ± 0.048
0.606TrpThr: 0.606 ± 0.04
0.899TrpVal: 0.899 ± 0.051
0.197TrpTrp: 0.197 ± 0.022
0.353TrpTyr: 0.353 ± 0.044
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.865TyrAla: 2.865 ± 0.097
0.305TyrCys: 0.305 ± 0.03
1.389TyrAsp: 1.389 ± 0.07
1.554TyrGlu: 1.554 ± 0.07
1.108TyrPhe: 1.108 ± 0.062
2.268TyrGly: 2.268 ± 0.095
0.391TyrHis: 0.391 ± 0.037
1.079TyrIle: 1.079 ± 0.059
0.738TyrLys: 0.738 ± 0.056
2.928TyrLeu: 2.928 ± 0.113
0.427TyrMet: 0.427 ± 0.036
0.681TyrAsn: 0.681 ± 0.049
1.368TyrPro: 1.368 ± 0.066
0.592TyrGln: 0.592 ± 0.044
2.336TyrArg: 2.336 ± 0.094
1.413TyrSer: 1.413 ± 0.071
1.494TyrThr: 1.494 ± 0.067
1.811TyrVal: 1.811 ± 0.073
0.356TyrTrp: 0.356 ± 0.034
0.726TyrTyr: 0.726 ± 0.062
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1003 proteins (334713 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski