Amino acid dipepetide frequency for Rhodobacter sp. CACIA14H1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.552AlaAla: 20.552 ± 0.242
1.156AlaCys: 1.156 ± 0.038
7.302AlaAsp: 7.302 ± 0.091
8.841AlaGlu: 8.841 ± 0.114
4.526AlaPhe: 4.526 ± 0.077
12.878AlaGly: 12.878 ± 0.203
2.458AlaHis: 2.458 ± 0.052
5.872AlaIle: 5.872 ± 0.081
3.819AlaLys: 3.819 ± 0.071
15.087AlaLeu: 15.087 ± 0.149
3.955AlaMet: 3.955 ± 0.068
2.47AlaAsn: 2.47 ± 0.048
6.253AlaPro: 6.253 ± 0.099
4.33AlaGln: 4.33 ± 0.067
9.992AlaArg: 9.992 ± 0.144
5.211AlaSer: 5.211 ± 0.071
6.895AlaThr: 6.895 ± 0.086
9.728AlaVal: 9.728 ± 0.129
1.491AlaTrp: 1.491 ± 0.042
2.463AlaTyr: 2.463 ± 0.048
0.001AlaXaa: 0.001 ± 0.001
Cys
1.05CysAla: 1.05 ± 0.031
0.103CysCys: 0.103 ± 0.011
0.617CysAsp: 0.617 ± 0.024
0.385CysGlu: 0.385 ± 0.019
0.327CysPhe: 0.327 ± 0.016
0.998CysGly: 0.998 ± 0.031
0.242CysHis: 0.242 ± 0.018
0.437CysIle: 0.437 ± 0.021
0.182CysLys: 0.182 ± 0.013
0.897CysLeu: 0.897 ± 0.032
0.178CysMet: 0.178 ± 0.012
0.2CysAsn: 0.2 ± 0.014
0.528CysPro: 0.528 ± 0.026
0.204CysGln: 0.204 ± 0.014
0.597CysArg: 0.597 ± 0.027
0.397CysSer: 0.397 ± 0.016
0.495CysThr: 0.495 ± 0.025
0.58CysVal: 0.58 ± 0.028
0.121CysTrp: 0.121 ± 0.011
0.176CysTyr: 0.176 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
7.382AspAla: 7.382 ± 0.094
0.521AspCys: 0.521 ± 0.024
3.014AspAsp: 3.014 ± 0.102
3.048AspGlu: 3.048 ± 0.057
2.194AspPhe: 2.194 ± 0.049
5.812AspGly: 5.812 ± 0.131
1.36AspHis: 1.36 ± 0.04
2.877AspIle: 2.877 ± 0.057
1.17AspLys: 1.17 ± 0.035
6.393AspLeu: 6.393 ± 0.085
1.604AspMet: 1.604 ± 0.039
1.011AspAsn: 1.011 ± 0.034
3.855AspPro: 3.855 ± 0.064
1.537AspGln: 1.537 ± 0.044
4.817AspArg: 4.817 ± 0.069
2.015AspSer: 2.015 ± 0.08
2.753AspThr: 2.753 ± 0.103
3.93AspVal: 3.93 ± 0.06
1.307AspTrp: 1.307 ± 0.038
1.449AspTyr: 1.449 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
8.121GluAla: 8.121 ± 0.115
0.324GluCys: 0.324 ± 0.019
2.783GluAsp: 2.783 ± 0.053
3.199GluGlu: 3.199 ± 0.069
1.632GluPhe: 1.632 ± 0.044
5.413GluGly: 5.413 ± 0.091
0.904GluHis: 0.904 ± 0.032
2.946GluIle: 2.946 ± 0.059
1.824GluLys: 1.824 ± 0.047
4.648GluLeu: 4.648 ± 0.072
1.68GluMet: 1.68 ± 0.038
1.323GluAsn: 1.323 ± 0.037
2.458GluPro: 2.458 ± 0.055
1.627GluGln: 1.627 ± 0.036
4.205GluArg: 4.205 ± 0.074
2.073GluSer: 2.073 ± 0.04
3.34GluThr: 3.34 ± 0.062
4.528GluVal: 4.528 ± 0.073
0.71GluTrp: 0.71 ± 0.024
0.905GluTyr: 0.905 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.704PheAla: 4.704 ± 0.071
0.406PheCys: 0.406 ± 0.018
2.715PheAsp: 2.715 ± 0.051
1.716PheGlu: 1.716 ± 0.039
1.382PhePhe: 1.382 ± 0.038
3.753PheGly: 3.753 ± 0.07
0.73PheHis: 0.73 ± 0.026
1.46PheIle: 1.46 ± 0.041
0.765PheLys: 0.765 ± 0.03
3.612PheLeu: 3.612 ± 0.066
0.768PheMet: 0.768 ± 0.029
0.902PheAsn: 0.902 ± 0.033
1.683PhePro: 1.683 ± 0.042
0.842PheGln: 0.842 ± 0.029
2.471PheArg: 2.471 ± 0.059
1.791PheSer: 1.791 ± 0.042
2.136PheThr: 2.136 ± 0.041
2.665PheVal: 2.665 ± 0.056
0.586PheTrp: 0.586 ± 0.026
0.873PheTyr: 0.873 ± 0.033
0.0PheXaa: 0.0 ± 0.0
Gly
10.947GlyAla: 10.947 ± 0.159
0.928GlyCys: 0.928 ± 0.029
4.776GlyAsp: 4.776 ± 0.145
4.728GlyGlu: 4.728 ± 0.079
3.816GlyPhe: 3.816 ± 0.075
8.356GlyGly: 8.356 ± 0.203
2.024GlyHis: 2.024 ± 0.047
4.549GlyIle: 4.549 ± 0.061
3.097GlyLys: 3.097 ± 0.071
9.73GlyLeu: 9.73 ± 0.126
2.915GlyMet: 2.915 ± 0.061
2.267GlyAsn: 2.267 ± 0.121
3.971GlyPro: 3.971 ± 0.067
3.11GlyGln: 3.11 ± 0.062
6.703GlyArg: 6.703 ± 0.094
4.06GlySer: 4.06 ± 0.063
5.297GlyThr: 5.297 ± 0.097
6.837GlyVal: 6.837 ± 0.101
1.735GlyTrp: 1.735 ± 0.048
2.277GlyTyr: 2.277 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.438HisAla: 2.438 ± 0.05
0.23HisCys: 0.23 ± 0.016
1.303HisAsp: 1.303 ± 0.04
0.949HisGlu: 0.949 ± 0.032
0.801HisPhe: 0.801 ± 0.027
2.071HisGly: 2.071 ± 0.048
0.626HisHis: 0.626 ± 0.026
0.899HisIle: 0.899 ± 0.029
0.373HisLys: 0.373 ± 0.019
2.262HisLeu: 2.262 ± 0.057
0.516HisMet: 0.516 ± 0.021
0.406HisAsn: 0.406 ± 0.02
1.55HisPro: 1.55 ± 0.043
0.472HisGln: 0.472 ± 0.019
1.494HisArg: 1.494 ± 0.041
0.867HisSer: 0.867 ± 0.034
0.708HisThr: 0.708 ± 0.025
1.543HisVal: 1.543 ± 0.04
0.389HisTrp: 0.389 ± 0.02
0.525HisTyr: 0.525 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.779IleAla: 6.779 ± 0.088
0.526IleCys: 0.526 ± 0.021
3.238IleAsp: 3.238 ± 0.067
2.762IleGlu: 2.762 ± 0.053
1.462IlePhe: 1.462 ± 0.045
4.512IleGly: 4.512 ± 0.072
0.939IleHis: 0.939 ± 0.033
1.85IleIle: 1.85 ± 0.053
1.026IleLys: 1.026 ± 0.038
4.668IleLeu: 4.668 ± 0.079
0.872IleMet: 0.872 ± 0.031
1.079IleAsn: 1.079 ± 0.035
2.429IlePro: 2.429 ± 0.054
0.933IleGln: 0.933 ± 0.035
3.472IleArg: 3.472 ± 0.055
2.313IleSer: 2.313 ± 0.049
2.783IleThr: 2.783 ± 0.052
3.587IleVal: 3.587 ± 0.063
0.572IleTrp: 0.572 ± 0.023
0.998IleTyr: 0.998 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.92LysAla: 3.92 ± 0.077
0.156LysCys: 0.156 ± 0.011
1.488LysAsp: 1.488 ± 0.044
1.302LysGlu: 1.302 ± 0.036
0.747LysPhe: 0.747 ± 0.028
2.889LysGly: 2.889 ± 0.063
0.497LysHis: 0.497 ± 0.023
1.218LysIle: 1.218 ± 0.039
0.98LysLys: 0.98 ± 0.035
2.613LysLeu: 2.613 ± 0.054
0.75LysMet: 0.75 ± 0.03
0.635LysAsn: 0.635 ± 0.025
1.676LysPro: 1.676 ± 0.05
0.767LysGln: 0.767 ± 0.03
1.903LysArg: 1.903 ± 0.051
1.468LysSer: 1.468 ± 0.04
1.568LysThr: 1.568 ± 0.043
2.261LysVal: 2.261 ± 0.05
0.366LysTrp: 0.366 ± 0.018
0.498LysTyr: 0.498 ± 0.025
0.001LysXaa: 0.001 ± 0.001
Leu
14.883LeuAla: 14.883 ± 0.161
0.978LeuCys: 0.978 ± 0.032
5.853LeuAsp: 5.853 ± 0.081
4.859LeuGlu: 4.859 ± 0.068
3.516LeuPhe: 3.516 ± 0.065
8.484LeuGly: 8.484 ± 0.1
2.022LeuHis: 2.022 ± 0.05
4.433LeuIle: 4.433 ± 0.072
2.905LeuLys: 2.905 ± 0.062
9.564LeuLeu: 9.564 ± 0.125
2.715LeuMet: 2.715 ± 0.053
2.313LeuAsn: 2.313 ± 0.041
6.276LeuPro: 6.276 ± 0.092
2.594LeuGln: 2.594 ± 0.055
7.901LeuArg: 7.901 ± 0.113
6.525LeuSer: 6.525 ± 0.092
6.158LeuThr: 6.158 ± 0.08
7.458LeuVal: 7.458 ± 0.095
1.527LeuTrp: 1.527 ± 0.045
1.956LeuTyr: 1.956 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
3.783MetAla: 3.783 ± 0.066
0.159MetCys: 0.159 ± 0.012
1.465MetAsp: 1.465 ± 0.036
1.423MetGlu: 1.423 ± 0.041
0.713MetPhe: 0.713 ± 0.026
2.454MetGly: 2.454 ± 0.052
0.447MetHis: 0.447 ± 0.023
1.337MetIle: 1.337 ± 0.038
0.959MetLys: 0.959 ± 0.033
2.49MetLeu: 2.49 ± 0.053
0.752MetMet: 0.752 ± 0.027
0.72MetAsn: 0.72 ± 0.026
1.54MetPro: 1.54 ± 0.039
0.989MetGln: 0.989 ± 0.031
1.96MetArg: 1.96 ± 0.04
1.427MetSer: 1.427 ± 0.035
2.071MetThr: 2.071 ± 0.041
2.1MetVal: 2.1 ± 0.046
0.254MetTrp: 0.254 ± 0.015
0.309MetTyr: 0.309 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
2.826AsnAla: 2.826 ± 0.058
0.215AsnCys: 0.215 ± 0.016
1.345AsnAsp: 1.345 ± 0.128
0.908AsnGlu: 0.908 ± 0.034
0.874AsnPhe: 0.874 ± 0.03
2.161AsnGly: 2.161 ± 0.048
0.498AsnHis: 0.498 ± 0.023
1.15AsnIle: 1.15 ± 0.036
0.477AsnLys: 0.477 ± 0.02
2.379AsnLeu: 2.379 ± 0.046
0.613AsnMet: 0.613 ± 0.023
0.506AsnAsn: 0.506 ± 0.02
1.741AsnPro: 1.741 ± 0.043
0.537AsnGln: 0.537 ± 0.024
1.713AsnArg: 1.713 ± 0.038
0.945AsnSer: 0.945 ± 0.029
1.057AsnThr: 1.057 ± 0.034
1.626AsnVal: 1.626 ± 0.044
0.372AsnTrp: 0.372 ± 0.019
0.555AsnTyr: 0.555 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
7.439ProAla: 7.439 ± 0.105
0.403ProCys: 0.403 ± 0.019
4.209ProAsp: 4.209 ± 0.072
3.862ProGlu: 3.862 ± 0.07
2.048ProPhe: 2.048 ± 0.049
4.52ProGly: 4.52 ± 0.078
1.119ProHis: 1.119 ± 0.039
1.839ProIle: 1.839 ± 0.042
1.614ProLys: 1.614 ± 0.048
5.0ProLeu: 5.0 ± 0.086
1.368ProMet: 1.368 ± 0.035
1.14ProAsn: 1.14 ± 0.032
2.682ProPro: 2.682 ± 0.063
1.764ProGln: 1.764 ± 0.048
3.116ProArg: 3.116 ± 0.06
2.419ProSer: 2.419 ± 0.048
2.368ProThr: 2.368 ± 0.055
5.074ProVal: 5.074 ± 0.08
0.74ProTrp: 0.74 ± 0.031
1.065ProTyr: 1.065 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.997GlnAla: 3.997 ± 0.068
0.182GlnCys: 0.182 ± 0.015
1.534GlnAsp: 1.534 ± 0.04
1.391GlnGlu: 1.391 ± 0.036
0.926GlnPhe: 0.926 ± 0.027
2.86GlnGly: 2.86 ± 0.052
0.545GlnHis: 0.545 ± 0.024
1.599GlnIle: 1.599 ± 0.043
0.925GlnLys: 0.925 ± 0.031
2.258GlnLeu: 2.258 ± 0.052
0.968GlnMet: 0.968 ± 0.031
0.721GlnAsn: 0.721 ± 0.027
1.638GlnPro: 1.638 ± 0.04
0.922GlnGln: 0.922 ± 0.035
2.146GlnArg: 2.146 ± 0.045
1.49GlnSer: 1.49 ± 0.037
1.569GlnThr: 1.569 ± 0.042
2.297GlnVal: 2.297 ± 0.053
0.39GlnTrp: 0.39 ± 0.019
0.496GlnTyr: 0.496 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
9.239ArgAla: 9.239 ± 0.122
0.51ArgCys: 0.51 ± 0.024
4.479ArgAsp: 4.479 ± 0.073
3.689ArgGlu: 3.689 ± 0.069
2.816ArgPhe: 2.816 ± 0.06
5.333ArgGly: 5.333 ± 0.075
1.692ArgHis: 1.692 ± 0.045
4.154ArgIle: 4.154 ± 0.065
2.074ArgLys: 2.074 ± 0.048
8.408ArgLeu: 8.408 ± 0.117
2.257ArgMet: 2.257 ± 0.047
1.686ArgAsn: 1.686 ± 0.04
3.873ArgPro: 3.873 ± 0.065
2.275ArgGln: 2.275 ± 0.052
5.615ArgArg: 5.615 ± 0.092
3.158ArgSer: 3.158 ± 0.06
3.285ArgThr: 3.285 ± 0.058
5.222ArgVal: 5.222 ± 0.085
1.083ArgTrp: 1.083 ± 0.033
1.433ArgTyr: 1.433 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
5.574SerAla: 5.574 ± 0.084
0.419SerCys: 0.419 ± 0.021
2.713SerAsp: 2.713 ± 0.055
2.211SerGlu: 2.211 ± 0.052
2.022SerPhe: 2.022 ± 0.047
5.031SerGly: 5.031 ± 0.088
0.949SerHis: 0.949 ± 0.033
2.134SerIle: 2.134 ± 0.05
1.089SerLys: 1.089 ± 0.035
4.793SerLeu: 4.793 ± 0.081
1.127SerMet: 1.127 ± 0.031
1.085SerAsn: 1.085 ± 0.037
2.501SerPro: 2.501 ± 0.046
1.293SerGln: 1.293 ± 0.037
3.181SerArg: 3.181 ± 0.056
2.114SerSer: 2.114 ± 0.052
2.322SerThr: 2.322 ± 0.059
3.764SerVal: 3.764 ± 0.062
0.596SerTrp: 0.596 ± 0.026
1.103SerTyr: 1.103 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
6.949ThrAla: 6.949 ± 0.089
0.473ThrCys: 0.473 ± 0.021
3.147ThrAsp: 3.147 ± 0.052
2.877ThrGlu: 2.877 ± 0.055
1.995ThrPhe: 1.995 ± 0.043
5.85ThrGly: 5.85 ± 0.1
1.164ThrHis: 1.164 ± 0.032
2.568ThrIle: 2.568 ± 0.058
1.239ThrLys: 1.239 ± 0.035
6.212ThrLeu: 6.212 ± 0.087
1.266ThrMet: 1.266 ± 0.035
1.165ThrAsn: 1.165 ± 0.041
3.354ThrPro: 3.354 ± 0.063
1.339ThrGln: 1.339 ± 0.038
3.615ThrArg: 3.615 ± 0.061
2.184ThrSer: 2.184 ± 0.052
2.859ThrThr: 2.859 ± 0.073
4.773ThrVal: 4.773 ± 0.07
0.694ThrTrp: 0.694 ± 0.027
1.132ThrTyr: 1.132 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
10.697ValAla: 10.697 ± 0.131
0.651ValCys: 0.651 ± 0.025
3.888ValAsp: 3.888 ± 0.07
4.681ValGlu: 4.681 ± 0.071
2.781ValPhe: 2.781 ± 0.057
5.601ValGly: 5.601 ± 0.09
1.357ValHis: 1.357 ± 0.035
4.022ValIle: 4.022 ± 0.065
2.255ValLys: 2.255 ± 0.049
8.034ValLeu: 8.034 ± 0.109
2.201ValMet: 2.201 ± 0.052
1.907ValAsn: 1.907 ± 0.05
3.94ValPro: 3.94 ± 0.062
2.17ValGln: 2.17 ± 0.049
4.589ValArg: 4.589 ± 0.07
3.756ValSer: 3.756 ± 0.054
5.379ValThr: 5.379 ± 0.079
6.199ValVal: 6.199 ± 0.089
1.194ValTrp: 1.194 ± 0.038
1.356ValTyr: 1.356 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.493TrpAla: 1.493 ± 0.04
0.153TrpCys: 0.153 ± 0.012
0.755TrpAsp: 0.755 ± 0.026
0.723TrpGlu: 0.723 ± 0.031
0.607TrpPhe: 0.607 ± 0.025
1.136TrpGly: 1.136 ± 0.032
0.378TrpHis: 0.378 ± 0.019
0.623TrpIle: 0.623 ± 0.024
0.474TrpLys: 0.474 ± 0.021
1.754TrpLeu: 1.754 ± 0.05
0.411TrpMet: 0.411 ± 0.019
0.447TrpAsn: 0.447 ± 0.021
0.788TrpPro: 0.788 ± 0.027
0.61TrpGln: 0.61 ± 0.024
1.217TrpArg: 1.217 ± 0.033
0.79TrpSer: 0.79 ± 0.03
0.796TrpThr: 0.796 ± 0.03
1.034TrpVal: 1.034 ± 0.037
0.262TrpTrp: 0.262 ± 0.018
0.273TrpTyr: 0.273 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.507TyrAla: 2.507 ± 0.048
0.221TyrCys: 0.221 ± 0.015
1.424TyrAsp: 1.424 ± 0.038
1.088TyrGlu: 1.088 ± 0.032
0.775TyrPhe: 0.775 ± 0.026
2.011TyrGly: 2.011 ± 0.051
0.469TyrHis: 0.469 ± 0.023
0.837TyrIle: 0.837 ± 0.029
0.455TyrLys: 0.455 ± 0.022
2.116TyrLeu: 2.116 ± 0.047
0.436TyrMet: 0.436 ± 0.022
0.513TyrAsn: 0.513 ± 0.023
1.052TyrPro: 1.052 ± 0.035
0.541TyrGln: 0.541 ± 0.022
1.573TyrArg: 1.573 ± 0.046
1.017TyrSer: 1.017 ± 0.032
1.016TyrThr: 1.016 ± 0.032
1.453TyrVal: 1.453 ± 0.039
0.339TyrTrp: 0.339 ± 0.02
0.506TyrTyr: 0.506 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.001XaaAla: 0.001 ± 0.001
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.001XaaLeu: 0.001 ± 0.001
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.001XaaXaa: 0.001 ± 0.001
Statistics based on 3570 proteins (1060311 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski