Amino acid dipepetide frequency for Bifidobacteriaceae bacterium NR015

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.944AlaAla: 9.944 ± 0.209
1.126AlaCys: 1.126 ± 0.059
5.451AlaAsp: 5.451 ± 0.118
5.516AlaGlu: 5.516 ± 0.135
3.257AlaPhe: 3.257 ± 0.086
6.735AlaGly: 6.735 ± 0.162
2.046AlaHis: 2.046 ± 0.066
5.822AlaIle: 5.822 ± 0.129
6.305AlaLys: 6.305 ± 0.162
9.549AlaLeu: 9.549 ± 0.166
2.581AlaMet: 2.581 ± 0.084
4.249AlaAsn: 4.249 ± 0.123
3.068AlaPro: 3.068 ± 0.107
4.481AlaGln: 4.481 ± 0.135
4.729AlaArg: 4.729 ± 0.133
6.385AlaSer: 6.385 ± 0.147
4.719AlaThr: 4.719 ± 0.125
7.119AlaVal: 7.119 ± 0.146
1.179AlaTrp: 1.179 ± 0.058
2.553AlaTyr: 2.553 ± 0.091
0.0AlaXaa: 0.0 ± 0.0
Cys
1.319CysAla: 1.319 ± 0.061
0.143CysCys: 0.143 ± 0.027
0.756CysAsp: 0.756 ± 0.048
0.776CysGlu: 0.776 ± 0.043
0.367CysPhe: 0.367 ± 0.032
1.058CysGly: 1.058 ± 0.064
0.171CysHis: 0.171 ± 0.017
0.628CysIle: 0.628 ± 0.044
0.588CysLys: 0.588 ± 0.042
0.746CysLeu: 0.746 ± 0.051
0.251CysMet: 0.251 ± 0.026
0.485CysAsn: 0.485 ± 0.038
0.432CysPro: 0.432 ± 0.035
0.264CysGln: 0.264 ± 0.031
0.369CysArg: 0.369 ± 0.027
0.686CysSer: 0.686 ± 0.035
0.588CysThr: 0.588 ± 0.038
0.985CysVal: 0.985 ± 0.055
0.106CysTrp: 0.106 ± 0.017
0.297CysTyr: 0.297 ± 0.028
0.0CysXaa: 0.0 ± 0.0
Asp
6.27AspAla: 6.27 ± 0.162
0.681AspCys: 0.681 ± 0.048
4.237AspAsp: 4.237 ± 0.148
4.765AspGlu: 4.765 ± 0.113
2.606AspPhe: 2.606 ± 0.074
4.41AspGly: 4.41 ± 0.118
0.975AspHis: 0.975 ± 0.053
3.8AspIle: 3.8 ± 0.093
3.038AspLys: 3.038 ± 0.128
5.031AspLeu: 5.031 ± 0.132
1.493AspMet: 1.493 ± 0.07
2.676AspAsn: 2.676 ± 0.082
2.578AspPro: 2.578 ± 0.08
1.357AspGln: 1.357 ± 0.061
2.335AspArg: 2.335 ± 0.118
4.4AspSer: 4.4 ± 0.115
2.978AspThr: 2.978 ± 0.096
4.498AspVal: 4.498 ± 0.117
0.779AspTrp: 0.779 ± 0.049
1.937AspTyr: 1.937 ± 0.075
0.0AspXaa: 0.0 ± 0.0
Glu
5.712GluAla: 5.712 ± 0.155
0.563GluCys: 0.563 ± 0.04
3.805GluAsp: 3.805 ± 0.1
4.121GluGlu: 4.121 ± 0.124
1.963GluPhe: 1.963 ± 0.073
3.511GluGly: 3.511 ± 0.104
1.764GluHis: 1.764 ± 0.077
3.523GluIle: 3.523 ± 0.114
3.546GluLys: 3.546 ± 0.106
5.4GluLeu: 5.4 ± 0.137
1.259GluMet: 1.259 ± 0.063
3.518GluAsn: 3.518 ± 0.102
2.126GluPro: 2.126 ± 0.084
2.493GluGln: 2.493 ± 0.086
3.501GluArg: 3.501 ± 0.128
4.048GluSer: 4.048 ± 0.127
3.174GluThr: 3.174 ± 0.095
4.008GluVal: 4.008 ± 0.096
0.563GluTrp: 0.563 ± 0.037
1.95GluTyr: 1.95 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
4.176PheAla: 4.176 ± 0.108
0.432PheCys: 0.432 ± 0.036
2.498PheAsp: 2.498 ± 0.08
1.968PheGlu: 1.968 ± 0.072
1.261PhePhe: 1.261 ± 0.069
3.114PheGly: 3.114 ± 0.097
0.626PheHis: 0.626 ± 0.044
2.247PheIle: 2.247 ± 0.091
1.651PheLys: 1.651 ± 0.066
2.739PheLeu: 2.739 ± 0.098
0.875PheMet: 0.875 ± 0.05
1.654PheAsn: 1.654 ± 0.061
1.244PhePro: 1.244 ± 0.054
0.895PheGln: 0.895 ± 0.047
1.39PheArg: 1.39 ± 0.06
2.641PheSer: 2.641 ± 0.086
2.161PheThr: 2.161 ± 0.08
3.023PheVal: 3.023 ± 0.1
0.362PheTrp: 0.362 ± 0.032
0.877PheTyr: 0.877 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
5.78GlyAla: 5.78 ± 0.136
0.731GlyCys: 0.731 ± 0.04
3.795GlyAsp: 3.795 ± 0.105
4.099GlyGlu: 4.099 ± 0.125
3.058GlyPhe: 3.058 ± 0.099
4.508GlyGly: 4.508 ± 0.134
1.322GlyHis: 1.322 ± 0.059
4.722GlyIle: 4.722 ± 0.108
4.526GlyLys: 4.526 ± 0.118
5.878GlyLeu: 5.878 ± 0.149
1.935GlyMet: 1.935 ± 0.063
2.92GlyAsn: 2.92 ± 0.089
1.774GlyPro: 1.774 ± 0.063
1.754GlyGln: 1.754 ± 0.069
3.279GlyArg: 3.279 ± 0.111
4.953GlySer: 4.953 ± 0.133
3.862GlyThr: 3.862 ± 0.098
5.506GlyVal: 5.506 ± 0.13
0.854GlyTrp: 0.854 ± 0.05
2.312GlyTyr: 2.312 ± 0.081
0.0GlyXaa: 0.0 ± 0.0
His
1.99HisAla: 1.99 ± 0.068
0.241HisCys: 0.241 ± 0.024
1.47HisAsp: 1.47 ± 0.064
1.267HisGlu: 1.267 ± 0.054
0.598HisPhe: 0.598 ± 0.036
1.545HisGly: 1.545 ± 0.062
0.53HisHis: 0.53 ± 0.037
1.382HisIle: 1.382 ± 0.067
1.106HisLys: 1.106 ± 0.056
1.503HisLeu: 1.503 ± 0.062
0.568HisMet: 0.568 ± 0.041
1.015HisAsn: 1.015 ± 0.044
1.101HisPro: 1.101 ± 0.05
0.53HisGln: 0.53 ± 0.032
1.071HisArg: 1.071 ± 0.051
1.302HisSer: 1.302 ± 0.051
1.239HisThr: 1.239 ± 0.053
1.729HisVal: 1.729 ± 0.067
0.264HisTrp: 0.264 ± 0.027
0.656HisTyr: 0.656 ± 0.045
0.0HisXaa: 0.0 ± 0.0
Ile
6.938IleAla: 6.938 ± 0.148
0.827IleCys: 0.827 ± 0.049
4.114IleAsp: 4.114 ± 0.1
3.651IleGlu: 3.651 ± 0.107
2.179IlePhe: 2.179 ± 0.088
4.395IleGly: 4.395 ± 0.112
1.111IleHis: 1.111 ± 0.055
3.905IleIle: 3.905 ± 0.124
2.709IleLys: 2.709 ± 0.087
4.749IleLeu: 4.749 ± 0.125
1.573IleMet: 1.573 ± 0.065
2.626IleAsn: 2.626 ± 0.095
2.855IlePro: 2.855 ± 0.086
1.51IleGln: 1.51 ± 0.059
2.983IleArg: 2.983 ± 0.082
4.822IleSer: 4.822 ± 0.12
3.631IleThr: 3.631 ± 0.094
5.398IleVal: 5.398 ± 0.13
0.568IleTrp: 0.568 ± 0.039
1.395IleTyr: 1.395 ± 0.059
0.0IleXaa: 0.0 ± 0.0
Lys
5.451LysAla: 5.451 ± 0.173
0.377LysCys: 0.377 ± 0.03
3.654LysAsp: 3.654 ± 0.143
3.267LysGlu: 3.267 ± 0.107
1.606LysPhe: 1.606 ± 0.062
3.209LysGly: 3.209 ± 0.09
1.367LysHis: 1.367 ± 0.063
3.071LysIle: 3.071 ± 0.093
3.714LysLys: 3.714 ± 0.144
4.963LysLeu: 4.963 ± 0.112
1.206LysMet: 1.206 ± 0.058
3.445LysAsn: 3.445 ± 0.137
2.689LysPro: 2.689 ± 0.091
2.35LysGln: 2.35 ± 0.092
3.081LysArg: 3.081 ± 0.104
4.224LysSer: 4.224 ± 0.132
3.315LysThr: 3.315 ± 0.119
3.624LysVal: 3.624 ± 0.084
0.598LysTrp: 0.598 ± 0.037
1.804LysTyr: 1.804 ± 0.077
0.0LysXaa: 0.0 ± 0.0
Leu
8.396LeuAla: 8.396 ± 0.154
1.123LeuCys: 1.123 ± 0.058
5.275LeuAsp: 5.275 ± 0.138
4.712LeuGlu: 4.712 ± 0.124
3.179LeuPhe: 3.179 ± 0.109
5.996LeuGly: 5.996 ± 0.115
2.058LeuHis: 2.058 ± 0.073
5.456LeuIle: 5.456 ± 0.131
4.546LeuLys: 4.546 ± 0.107
7.913LeuLeu: 7.913 ± 0.162
2.048LeuMet: 2.048 ± 0.068
3.717LeuAsn: 3.717 ± 0.095
4.182LeuPro: 4.182 ± 0.12
3.372LeuGln: 3.372 ± 0.093
5.247LeuArg: 5.247 ± 0.13
6.405LeuSer: 6.405 ± 0.123
4.935LeuThr: 4.935 ± 0.108
6.061LeuVal: 6.061 ± 0.141
0.927LeuTrp: 0.927 ± 0.057
2.073LeuTyr: 2.073 ± 0.071
0.0LeuXaa: 0.0 ± 0.0
Met
2.237MetAla: 2.237 ± 0.082
0.314MetCys: 0.314 ± 0.027
1.191MetAsp: 1.191 ± 0.059
1.02MetGlu: 1.02 ± 0.05
0.935MetPhe: 0.935 ± 0.052
1.377MetGly: 1.377 ± 0.059
0.575MetHis: 0.575 ± 0.043
1.48MetIle: 1.48 ± 0.062
1.284MetLys: 1.284 ± 0.052
2.576MetLeu: 2.576 ± 0.074
0.671MetMet: 0.671 ± 0.044
1.133MetAsn: 1.133 ± 0.049
1.362MetPro: 1.362 ± 0.057
1.076MetGln: 1.076 ± 0.065
1.679MetArg: 1.679 ± 0.075
1.834MetSer: 1.834 ± 0.072
1.359MetThr: 1.359 ± 0.061
1.769MetVal: 1.769 ± 0.068
0.261MetTrp: 0.261 ± 0.028
0.684MetTyr: 0.684 ± 0.044
0.0MetXaa: 0.0 ± 0.0
Asn
4.616AsnAla: 4.616 ± 0.145
0.475AsnCys: 0.475 ± 0.042
2.689AsnAsp: 2.689 ± 0.101
2.691AsnGlu: 2.691 ± 0.088
1.354AsnPhe: 1.354 ± 0.063
3.209AsnGly: 3.209 ± 0.101
0.872AsnHis: 0.872 ± 0.045
3.038AsnIle: 3.038 ± 0.094
2.955AsnLys: 2.955 ± 0.11
3.666AsnLeu: 3.666 ± 0.102
1.194AsnMet: 1.194 ± 0.055
3.149AsnAsn: 3.149 ± 0.135
2.531AsnPro: 2.531 ± 0.085
1.621AsnGln: 1.621 ± 0.068
2.068AsnArg: 2.068 ± 0.077
3.616AsnSer: 3.616 ± 0.12
2.608AsnThr: 2.608 ± 0.1
3.186AsnVal: 3.186 ± 0.09
0.533AsnTrp: 0.533 ± 0.039
1.405AsnTyr: 1.405 ± 0.075
0.0AsnXaa: 0.0 ± 0.0
Pro
3.45ProAla: 3.45 ± 0.121
0.304ProCys: 0.304 ± 0.025
2.48ProAsp: 2.48 ± 0.088
3.056ProGlu: 3.056 ± 0.096
1.493ProPhe: 1.493 ± 0.061
2.631ProGly: 2.631 ± 0.092
0.857ProHis: 0.857 ± 0.05
2.43ProIle: 2.43 ± 0.073
2.309ProLys: 2.309 ± 0.075
3.337ProLeu: 3.337 ± 0.098
0.935ProMet: 0.935 ± 0.055
1.819ProAsn: 1.819 ± 0.073
0.92ProPro: 0.92 ± 0.051
1.563ProGln: 1.563 ± 0.087
1.669ProArg: 1.669 ± 0.063
2.701ProSer: 2.701 ± 0.092
2.47ProThr: 2.47 ± 0.085
3.297ProVal: 3.297 ± 0.105
0.573ProTrp: 0.573 ± 0.038
1.347ProTyr: 1.347 ± 0.064
0.0ProXaa: 0.0 ± 0.0
Gln
3.35GlnAla: 3.35 ± 0.094
0.357GlnCys: 0.357 ± 0.032
1.829GlnAsp: 1.829 ± 0.076
2.111GlnGlu: 2.111 ± 0.079
1.148GlnPhe: 1.148 ± 0.062
2.053GlnGly: 2.053 ± 0.079
0.829GlnHis: 0.829 ± 0.042
2.231GlnIle: 2.231 ± 0.076
1.917GlnLys: 1.917 ± 0.081
3.164GlnLeu: 3.164 ± 0.095
0.852GlnMet: 0.852 ± 0.045
1.865GlnAsn: 1.865 ± 0.079
1.347GlnPro: 1.347 ± 0.098
1.915GlnGln: 1.915 ± 0.132
2.02GlnArg: 2.02 ± 0.074
2.407GlnSer: 2.407 ± 0.087
1.799GlnThr: 1.799 ± 0.064
2.395GlnVal: 2.395 ± 0.09
0.52GlnTrp: 0.52 ± 0.042
1.274GlnTyr: 1.274 ± 0.085
0.0GlnXaa: 0.0 ± 0.0
Arg
4.486ArgAla: 4.486 ± 0.108
0.44ArgCys: 0.44 ± 0.032
2.857ArgAsp: 2.857 ± 0.133
3.707ArgGlu: 3.707 ± 0.118
1.917ArgPhe: 1.917 ± 0.067
2.948ArgGly: 2.948 ± 0.098
1.045ArgHis: 1.045 ± 0.054
3.392ArgIle: 3.392 ± 0.084
3.317ArgLys: 3.317 ± 0.099
4.445ArgLeu: 4.445 ± 0.114
1.49ArgMet: 1.49 ± 0.061
2.289ArgAsn: 2.289 ± 0.087
1.696ArgPro: 1.696 ± 0.071
1.561ArgGln: 1.561 ± 0.065
3.136ArgArg: 3.136 ± 0.149
3.008ArgSer: 3.008 ± 0.089
2.699ArgThr: 2.699 ± 0.08
3.948ArgVal: 3.948 ± 0.113
0.631ArgTrp: 0.631 ± 0.043
1.709ArgTyr: 1.709 ± 0.063
0.0ArgXaa: 0.0 ± 0.0
Ser
6.604SerAla: 6.604 ± 0.159
0.789SerCys: 0.789 ± 0.051
4.192SerAsp: 4.192 ± 0.103
4.247SerGlu: 4.247 ± 0.108
2.443SerPhe: 2.443 ± 0.098
5.157SerGly: 5.157 ± 0.132
1.53SerHis: 1.53 ± 0.056
4.315SerIle: 4.315 ± 0.114
4.307SerLys: 4.307 ± 0.124
6.154SerLeu: 6.154 ± 0.134
1.792SerMet: 1.792 ± 0.064
3.501SerAsn: 3.501 ± 0.114
2.103SerPro: 2.103 ± 0.082
2.835SerGln: 2.835 ± 0.087
3.513SerArg: 3.513 ± 0.104
5.81SerSer: 5.81 ± 0.166
3.744SerThr: 3.744 ± 0.108
5.237SerVal: 5.237 ± 0.127
0.867SerTrp: 0.867 ± 0.056
1.995SerTyr: 1.995 ± 0.085
0.0SerXaa: 0.0 ± 0.0
Thr
4.757ThrAla: 4.757 ± 0.133
0.58ThrCys: 0.58 ± 0.04
3.078ThrAsp: 3.078 ± 0.098
2.613ThrGlu: 2.613 ± 0.085
2.098ThrPhe: 2.098 ± 0.066
3.965ThrGly: 3.965 ± 0.096
1.239ThrHis: 1.239 ± 0.056
3.385ThrIle: 3.385 ± 0.104
3.091ThrLys: 3.091 ± 0.103
5.224ThrLeu: 5.224 ± 0.113
1.206ThrMet: 1.206 ± 0.058
2.528ThrAsn: 2.528 ± 0.089
2.573ThrPro: 2.573 ± 0.077
2.219ThrGln: 2.219 ± 0.094
2.596ThrArg: 2.596 ± 0.082
3.666ThrSer: 3.666 ± 0.097
3.307ThrThr: 3.307 ± 0.119
4.596ThrVal: 4.596 ± 0.118
0.616ThrTrp: 0.616 ± 0.046
1.545ThrTyr: 1.545 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
7.333ValAla: 7.333 ± 0.156
0.95ValCys: 0.95 ± 0.047
4.865ValAsp: 4.865 ± 0.112
4.563ValGlu: 4.563 ± 0.123
2.774ValPhe: 2.774 ± 0.083
4.672ValGly: 4.672 ± 0.126
1.4ValHis: 1.4 ± 0.055
4.845ValIle: 4.845 ± 0.104
3.81ValLys: 3.81 ± 0.106
7.066ValLeu: 7.066 ± 0.14
1.844ValMet: 1.844 ± 0.069
3.066ValAsn: 3.066 ± 0.1
3.536ValPro: 3.536 ± 0.099
2.159ValGln: 2.159 ± 0.067
3.807ValArg: 3.807 ± 0.109
5.644ValSer: 5.644 ± 0.13
4.335ValThr: 4.335 ± 0.104
5.792ValVal: 5.792 ± 0.152
0.764ValTrp: 0.764 ± 0.041
1.855ValTyr: 1.855 ± 0.068
0.0ValXaa: 0.0 ± 0.0
Trp
0.905TrpAla: 0.905 ± 0.057
0.161TrpCys: 0.161 ± 0.02
0.618TrpAsp: 0.618 ± 0.036
0.533TrpGlu: 0.533 ± 0.035
0.503TrpPhe: 0.503 ± 0.036
0.721TrpGly: 0.721 ± 0.045
0.297TrpHis: 0.297 ± 0.031
0.754TrpIle: 0.754 ± 0.046
0.648TrpLys: 0.648 ± 0.037
1.186TrpLeu: 1.186 ± 0.059
0.412TrpMet: 0.412 ± 0.037
0.57TrpAsn: 0.57 ± 0.042
0.407TrpPro: 0.407 ± 0.037
0.565TrpGln: 0.565 ± 0.04
0.746TrpArg: 0.746 ± 0.046
0.729TrpSer: 0.729 ± 0.04
0.54TrpThr: 0.54 ± 0.036
0.658TrpVal: 0.658 ± 0.044
0.219TrpTrp: 0.219 ± 0.021
0.397TrpTyr: 0.397 ± 0.032
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.101TyrAla: 3.101 ± 0.105
0.402TyrCys: 0.402 ± 0.033
1.99TyrAsp: 1.99 ± 0.076
1.865TyrGlu: 1.865 ± 0.075
1.093TyrPhe: 1.093 ± 0.059
2.355TyrGly: 2.355 ± 0.081
0.493TyrHis: 0.493 ± 0.033
1.513TyrIle: 1.513 ± 0.063
1.648TyrLys: 1.648 ± 0.07
2.329TyrLeu: 2.329 ± 0.082
0.633TyrMet: 0.633 ± 0.045
1.251TyrAsn: 1.251 ± 0.06
1.118TyrPro: 1.118 ± 0.056
0.867TyrGln: 0.867 ± 0.049
1.503TyrArg: 1.503 ± 0.068
1.847TyrSer: 1.847 ± 0.07
1.42TyrThr: 1.42 ± 0.068
2.249TyrVal: 2.249 ± 0.075
0.387TyrTrp: 0.387 ± 0.034
0.927TyrTyr: 0.927 ± 0.056
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1751 proteins (397942 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski