Amino acid dipepetide frequency for Brochothrix phage NF5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.759AlaAla: 4.759 ± 0.869
0.41AlaCys: 0.41 ± 0.166
4.02AlaAsp: 4.02 ± 0.618
6.154AlaGlu: 6.154 ± 0.953
3.2AlaPhe: 3.2 ± 0.524
3.036AlaGly: 3.036 ± 0.501
0.738AlaHis: 0.738 ± 0.239
5.579AlaIle: 5.579 ± 0.77
4.677AlaLys: 4.677 ± 0.568
5.087AlaLeu: 5.087 ± 0.842
1.067AlaMet: 1.067 ± 0.287
4.431AlaAsn: 4.431 ± 0.623
1.887AlaPro: 1.887 ± 0.414
3.118AlaGln: 3.118 ± 0.47
2.133AlaArg: 2.133 ± 0.47
3.282AlaSer: 3.282 ± 0.49
5.415AlaThr: 5.415 ± 0.843
4.431AlaVal: 4.431 ± 0.521
0.985AlaTrp: 0.985 ± 0.278
1.969AlaTyr: 1.969 ± 0.417
0.0AlaXaa: 0.0 ± 0.0
Cys
0.656CysAla: 0.656 ± 0.193
0.082CysCys: 0.082 ± 0.079
0.656CysAsp: 0.656 ± 0.225
0.738CysGlu: 0.738 ± 0.218
0.41CysPhe: 0.41 ± 0.151
0.41CysGly: 0.41 ± 0.207
0.0CysHis: 0.0 ± 0.0
0.41CysIle: 0.41 ± 0.184
0.903CysLys: 0.903 ± 0.298
0.574CysLeu: 0.574 ± 0.247
0.0CysMet: 0.0 ± 0.0
0.164CysAsn: 0.164 ± 0.113
0.41CysPro: 0.41 ± 0.176
0.246CysGln: 0.246 ± 0.152
0.246CysArg: 0.246 ± 0.145
0.574CysSer: 0.574 ± 0.232
0.328CysThr: 0.328 ± 0.144
0.41CysVal: 0.41 ± 0.189
0.0CysTrp: 0.0 ± 0.0
0.328CysTyr: 0.328 ± 0.158
0.0CysXaa: 0.0 ± 0.0
Asp
4.677AspAla: 4.677 ± 0.664
0.41AspCys: 0.41 ± 0.178
4.923AspAsp: 4.923 ± 0.707
5.743AspGlu: 5.743 ± 0.87
3.036AspPhe: 3.036 ± 0.478
4.595AspGly: 4.595 ± 0.662
0.328AspHis: 0.328 ± 0.156
5.251AspIle: 5.251 ± 0.639
6.072AspLys: 6.072 ± 0.719
4.923AspLeu: 4.923 ± 0.721
1.477AspMet: 1.477 ± 0.375
4.841AspAsn: 4.841 ± 0.603
1.559AspPro: 1.559 ± 0.491
1.313AspGln: 1.313 ± 0.278
1.887AspArg: 1.887 ± 0.316
2.872AspSer: 2.872 ± 0.652
3.528AspThr: 3.528 ± 0.475
4.431AspVal: 4.431 ± 0.603
0.985AspTrp: 0.985 ± 0.267
2.79AspTyr: 2.79 ± 0.472
0.0AspXaa: 0.0 ± 0.0
Glu
3.856GluAla: 3.856 ± 0.657
0.574GluCys: 0.574 ± 0.28
4.595GluAsp: 4.595 ± 0.606
4.923GluGlu: 4.923 ± 0.875
2.543GluPhe: 2.543 ± 0.54
3.446GluGly: 3.446 ± 0.527
0.985GluHis: 0.985 ± 0.279
5.169GluIle: 5.169 ± 0.806
6.072GluLys: 6.072 ± 0.832
7.302GluLeu: 7.302 ± 1.064
1.805GluMet: 1.805 ± 0.369
3.2GluAsn: 3.2 ± 0.536
2.133GluPro: 2.133 ± 0.515
3.856GluGln: 3.856 ± 0.68
3.118GluArg: 3.118 ± 0.619
3.938GluSer: 3.938 ± 0.608
5.169GluThr: 5.169 ± 0.631
7.302GluVal: 7.302 ± 0.805
1.395GluTrp: 1.395 ± 0.334
2.461GluTyr: 2.461 ± 0.422
0.0GluXaa: 0.0 ± 0.0
Phe
2.626PheAla: 2.626 ± 0.34
0.492PheCys: 0.492 ± 0.23
3.118PheAsp: 3.118 ± 0.413
2.79PheGlu: 2.79 ± 0.434
1.723PhePhe: 1.723 ± 0.417
2.543PheGly: 2.543 ± 0.578
0.41PheHis: 0.41 ± 0.173
2.872PheIle: 2.872 ± 0.341
5.333PheLys: 5.333 ± 0.659
2.954PheLeu: 2.954 ± 0.472
1.149PheMet: 1.149 ± 0.275
2.79PheAsn: 2.79 ± 0.417
0.656PhePro: 0.656 ± 0.222
1.067PheGln: 1.067 ± 0.364
1.313PheArg: 1.313 ± 0.366
2.79PheSer: 2.79 ± 0.4
2.379PheThr: 2.379 ± 0.435
3.118PheVal: 3.118 ± 0.613
0.492PheTrp: 0.492 ± 0.21
2.215PheTyr: 2.215 ± 0.446
0.0PheXaa: 0.0 ± 0.0
Gly
4.431GlyAla: 4.431 ± 0.637
0.328GlyCys: 0.328 ± 0.153
3.364GlyAsp: 3.364 ± 0.593
4.349GlyGlu: 4.349 ± 0.626
2.543GlyPhe: 2.543 ± 0.533
4.349GlyGly: 4.349 ± 1.188
1.149GlyHis: 1.149 ± 0.299
4.266GlyIle: 4.266 ± 0.699
6.154GlyLys: 6.154 ± 0.589
4.184GlyLeu: 4.184 ± 0.628
1.559GlyMet: 1.559 ± 0.491
2.872GlyAsn: 2.872 ± 0.565
1.231GlyPro: 1.231 ± 0.472
1.723GlyGln: 1.723 ± 0.456
1.969GlyArg: 1.969 ± 0.384
4.677GlySer: 4.677 ± 0.771
3.938GlyThr: 3.938 ± 0.604
5.087GlyVal: 5.087 ± 0.558
1.067GlyTrp: 1.067 ± 0.337
2.79GlyTyr: 2.79 ± 0.476
0.0GlyXaa: 0.0 ± 0.0
His
0.738HisAla: 0.738 ± 0.358
0.246HisCys: 0.246 ± 0.129
0.903HisAsp: 0.903 ± 0.255
0.903HisGlu: 0.903 ± 0.303
0.738HisPhe: 0.738 ± 0.31
1.067HisGly: 1.067 ± 0.291
0.41HisHis: 0.41 ± 0.258
1.149HisIle: 1.149 ± 0.341
1.231HisLys: 1.231 ± 0.28
0.738HisLeu: 0.738 ± 0.188
0.41HisMet: 0.41 ± 0.165
0.656HisAsn: 0.656 ± 0.216
0.492HisPro: 0.492 ± 0.245
0.246HisGln: 0.246 ± 0.129
0.574HisArg: 0.574 ± 0.282
1.641HisSer: 1.641 ± 0.383
0.738HisThr: 0.738 ± 0.226
0.82HisVal: 0.82 ± 0.241
0.082HisTrp: 0.082 ± 0.092
0.985HisTyr: 0.985 ± 0.233
0.0HisXaa: 0.0 ± 0.0
Ile
5.005IleAla: 5.005 ± 0.702
0.246IleCys: 0.246 ± 0.133
5.825IleAsp: 5.825 ± 0.72
6.728IleGlu: 6.728 ± 0.593
2.872IlePhe: 2.872 ± 0.546
4.431IleGly: 4.431 ± 0.859
1.149IleHis: 1.149 ± 0.274
3.528IleIle: 3.528 ± 0.624
6.4IleLys: 6.4 ± 0.789
3.282IleLeu: 3.282 ± 0.519
0.985IleMet: 0.985 ± 0.327
5.579IleAsn: 5.579 ± 0.949
2.215IlePro: 2.215 ± 0.331
2.215IleGln: 2.215 ± 0.504
1.723IleArg: 1.723 ± 0.307
4.841IleSer: 4.841 ± 0.568
6.072IleThr: 6.072 ± 0.815
4.266IleVal: 4.266 ± 0.6
0.656IleTrp: 0.656 ± 0.281
2.297IleTyr: 2.297 ± 0.439
0.0IleXaa: 0.0 ± 0.0
Lys
7.22LysAla: 7.22 ± 1.021
0.656LysCys: 0.656 ± 0.274
5.087LysAsp: 5.087 ± 0.772
7.959LysGlu: 7.959 ± 1.067
3.774LysPhe: 3.774 ± 0.552
6.892LysGly: 6.892 ± 0.861
1.969LysHis: 1.969 ± 0.423
6.4LysIle: 6.4 ± 0.807
8.615LysLys: 8.615 ± 0.965
6.236LysLeu: 6.236 ± 0.812
3.528LysMet: 3.528 ± 0.515
4.759LysAsn: 4.759 ± 0.997
1.805LysPro: 1.805 ± 0.388
4.102LysGln: 4.102 ± 0.513
4.102LysArg: 4.102 ± 0.555
3.938LysSer: 3.938 ± 0.685
5.661LysThr: 5.661 ± 0.856
6.072LysVal: 6.072 ± 0.861
1.313LysTrp: 1.313 ± 0.331
2.954LysTyr: 2.954 ± 0.428
0.0LysXaa: 0.0 ± 0.0
Leu
5.005LeuAla: 5.005 ± 0.847
0.656LeuCys: 0.656 ± 0.222
4.266LeuAsp: 4.266 ± 0.598
5.989LeuGlu: 5.989 ± 0.912
3.774LeuPhe: 3.774 ± 0.604
5.005LeuGly: 5.005 ± 0.641
1.231LeuHis: 1.231 ± 0.273
5.087LeuIle: 5.087 ± 0.709
9.107LeuLys: 9.107 ± 0.984
7.466LeuLeu: 7.466 ± 0.586
1.805LeuMet: 1.805 ± 0.418
4.102LeuAsn: 4.102 ± 0.478
1.477LeuPro: 1.477 ± 0.343
2.297LeuGln: 2.297 ± 0.384
3.2LeuArg: 3.2 ± 0.485
5.579LeuSer: 5.579 ± 0.746
5.415LeuThr: 5.415 ± 0.661
4.184LeuVal: 4.184 ± 0.552
0.164LeuTrp: 0.164 ± 0.113
2.461LeuTyr: 2.461 ± 0.472
0.0LeuXaa: 0.0 ± 0.0
Met
1.805MetAla: 1.805 ± 0.409
0.082MetCys: 0.082 ± 0.08
0.903MetAsp: 0.903 ± 0.263
1.149MetGlu: 1.149 ± 0.275
1.231MetPhe: 1.231 ± 0.296
0.985MetGly: 0.985 ± 0.549
0.574MetHis: 0.574 ± 0.243
1.231MetIle: 1.231 ± 0.331
2.708MetLys: 2.708 ± 0.497
1.477MetLeu: 1.477 ± 0.236
0.328MetMet: 0.328 ± 0.146
1.969MetAsn: 1.969 ± 0.402
0.82MetPro: 0.82 ± 0.231
0.903MetGln: 0.903 ± 0.267
1.313MetArg: 1.313 ± 0.312
2.133MetSer: 2.133 ± 0.391
2.133MetThr: 2.133 ± 0.495
1.231MetVal: 1.231 ± 0.338
0.41MetTrp: 0.41 ± 0.183
0.492MetTyr: 0.492 ± 0.212
0.0MetXaa: 0.0 ± 0.0
Asn
4.184AsnAla: 4.184 ± 0.548
0.574AsnCys: 0.574 ± 0.198
3.036AsnAsp: 3.036 ± 0.338
3.61AsnGlu: 3.61 ± 0.526
2.543AsnPhe: 2.543 ± 0.489
5.087AsnGly: 5.087 ± 0.603
0.903AsnHis: 0.903 ± 0.2
3.61AsnIle: 3.61 ± 0.578
5.087AsnLys: 5.087 ± 0.779
4.02AsnLeu: 4.02 ± 0.511
1.231AsnMet: 1.231 ± 0.384
2.872AsnAsn: 2.872 ± 0.517
1.641AsnPro: 1.641 ± 0.539
2.379AsnGln: 2.379 ± 0.568
2.872AsnArg: 2.872 ± 0.438
3.856AsnSer: 3.856 ± 0.625
3.364AsnThr: 3.364 ± 0.71
3.282AsnVal: 3.282 ± 0.68
0.492AsnTrp: 0.492 ± 0.199
2.297AsnTyr: 2.297 ± 0.414
0.0AsnXaa: 0.0 ± 0.0
Pro
1.641ProAla: 1.641 ± 0.433
0.164ProCys: 0.164 ± 0.116
1.477ProAsp: 1.477 ± 0.317
1.723ProGlu: 1.723 ± 0.427
1.559ProPhe: 1.559 ± 0.485
1.067ProGly: 1.067 ± 0.481
0.656ProHis: 0.656 ± 0.238
1.149ProIle: 1.149 ± 0.304
2.626ProLys: 2.626 ± 0.5
2.626ProLeu: 2.626 ± 0.437
1.067ProMet: 1.067 ± 0.266
1.067ProAsn: 1.067 ± 0.337
0.985ProPro: 0.985 ± 0.337
0.41ProGln: 0.41 ± 0.179
1.313ProArg: 1.313 ± 0.457
1.641ProSer: 1.641 ± 0.33
1.477ProThr: 1.477 ± 0.419
2.461ProVal: 2.461 ± 0.471
0.164ProTrp: 0.164 ± 0.117
0.82ProTyr: 0.82 ± 0.239
0.0ProXaa: 0.0 ± 0.0
Gln
2.379GlnAla: 2.379 ± 0.437
0.41GlnCys: 0.41 ± 0.184
2.461GlnAsp: 2.461 ± 0.517
2.297GlnGlu: 2.297 ± 0.418
1.559GlnPhe: 1.559 ± 0.283
2.133GlnGly: 2.133 ± 0.366
0.492GlnHis: 0.492 ± 0.176
2.872GlnIle: 2.872 ± 0.491
3.446GlnLys: 3.446 ± 0.52
3.692GlnLeu: 3.692 ± 0.429
0.903GlnMet: 0.903 ± 0.189
1.559GlnAsn: 1.559 ± 0.417
1.313GlnPro: 1.313 ± 0.366
2.79GlnGln: 2.79 ± 0.628
1.231GlnArg: 1.231 ± 0.265
2.543GlnSer: 2.543 ± 0.46
2.215GlnThr: 2.215 ± 0.419
1.969GlnVal: 1.969 ± 0.321
0.246GlnTrp: 0.246 ± 0.156
1.231GlnTyr: 1.231 ± 0.294
0.0GlnXaa: 0.0 ± 0.0
Arg
2.133ArgAla: 2.133 ± 0.393
0.164ArgCys: 0.164 ± 0.108
2.215ArgAsp: 2.215 ± 0.344
3.036ArgGlu: 3.036 ± 0.487
1.477ArgPhe: 1.477 ± 0.296
2.461ArgGly: 2.461 ± 0.441
0.574ArgHis: 0.574 ± 0.24
3.036ArgIle: 3.036 ± 0.614
3.774ArgLys: 3.774 ± 0.511
3.774ArgLeu: 3.774 ± 0.452
0.985ArgMet: 0.985 ± 0.265
2.626ArgAsn: 2.626 ± 0.466
1.149ArgPro: 1.149 ± 0.404
0.985ArgGln: 0.985 ± 0.346
1.477ArgArg: 1.477 ± 0.285
2.133ArgSer: 2.133 ± 0.418
1.559ArgThr: 1.559 ± 0.344
1.969ArgVal: 1.969 ± 0.396
0.492ArgTrp: 0.492 ± 0.188
2.215ArgTyr: 2.215 ± 0.479
0.0ArgXaa: 0.0 ± 0.0
Ser
3.364SerAla: 3.364 ± 0.413
0.328SerCys: 0.328 ± 0.147
5.087SerAsp: 5.087 ± 0.7
3.446SerGlu: 3.446 ± 0.5
2.297SerPhe: 2.297 ± 0.339
3.692SerGly: 3.692 ± 0.665
0.903SerHis: 0.903 ± 0.358
5.333SerIle: 5.333 ± 0.699
4.923SerLys: 4.923 ± 0.651
5.743SerLeu: 5.743 ± 0.516
1.641SerMet: 1.641 ± 0.344
4.102SerAsn: 4.102 ± 0.716
1.723SerPro: 1.723 ± 0.389
2.79SerGln: 2.79 ± 0.475
2.79SerArg: 2.79 ± 0.487
3.856SerSer: 3.856 ± 0.603
3.2SerThr: 3.2 ± 0.575
3.61SerVal: 3.61 ± 0.423
0.41SerTrp: 0.41 ± 0.172
2.708SerTyr: 2.708 ± 0.493
0.0SerXaa: 0.0 ± 0.0
Thr
4.513ThrAla: 4.513 ± 0.974
0.492ThrCys: 0.492 ± 0.185
4.677ThrAsp: 4.677 ± 0.658
4.431ThrGlu: 4.431 ± 0.74
2.461ThrPhe: 2.461 ± 0.334
4.431ThrGly: 4.431 ± 0.728
0.82ThrHis: 0.82 ± 0.304
4.595ThrIle: 4.595 ± 0.598
5.661ThrLys: 5.661 ± 0.712
5.743ThrLeu: 5.743 ± 0.605
1.149ThrMet: 1.149 ± 0.296
3.036ThrAsn: 3.036 ± 0.498
1.723ThrPro: 1.723 ± 0.362
2.543ThrGln: 2.543 ± 0.381
2.79ThrArg: 2.79 ± 0.525
3.446ThrSer: 3.446 ± 0.501
5.497ThrThr: 5.497 ± 0.953
4.677ThrVal: 4.677 ± 0.611
0.41ThrTrp: 0.41 ± 0.216
1.969ThrTyr: 1.969 ± 0.341
0.0ThrXaa: 0.0 ± 0.0
Val
4.102ValAla: 4.102 ± 0.64
0.903ValCys: 0.903 ± 0.304
5.087ValAsp: 5.087 ± 0.624
3.938ValGlu: 3.938 ± 0.45
3.036ValPhe: 3.036 ± 0.396
3.774ValGly: 3.774 ± 0.628
0.492ValHis: 0.492 ± 0.177
4.595ValIle: 4.595 ± 0.575
6.236ValLys: 6.236 ± 0.763
4.513ValLeu: 4.513 ± 0.446
1.477ValMet: 1.477 ± 0.352
3.774ValAsn: 3.774 ± 0.6
2.215ValPro: 2.215 ± 0.403
2.543ValGln: 2.543 ± 0.458
2.051ValArg: 2.051 ± 0.408
5.169ValSer: 5.169 ± 0.655
4.595ValThr: 4.595 ± 0.654
5.251ValVal: 5.251 ± 0.554
0.41ValTrp: 0.41 ± 0.203
3.118ValTyr: 3.118 ± 0.663
0.0ValXaa: 0.0 ± 0.0
Trp
0.328TrpAla: 0.328 ± 0.189
0.082TrpCys: 0.082 ± 0.085
0.492TrpAsp: 0.492 ± 0.261
1.231TrpGlu: 1.231 ± 0.277
0.328TrpPhe: 0.328 ± 0.17
0.41TrpGly: 0.41 ± 0.22
0.41TrpHis: 0.41 ± 0.169
1.313TrpIle: 1.313 ± 0.33
0.656TrpLys: 0.656 ± 0.266
0.985TrpLeu: 0.985 ± 0.276
0.328TrpMet: 0.328 ± 0.128
0.738TrpAsn: 0.738 ± 0.244
0.0TrpPro: 0.0 ± 0.0
0.246TrpGln: 0.246 ± 0.123
0.41TrpArg: 0.41 ± 0.181
1.067TrpSer: 1.067 ± 0.25
0.82TrpThr: 0.82 ± 0.247
0.41TrpVal: 0.41 ± 0.194
0.0TrpTrp: 0.0 ± 0.0
0.246TrpTyr: 0.246 ± 0.132
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.954TyrAla: 2.954 ± 0.526
0.246TyrCys: 0.246 ± 0.14
3.446TyrAsp: 3.446 ± 0.501
2.708TyrGlu: 2.708 ± 0.462
1.723TyrPhe: 1.723 ± 0.359
2.215TyrGly: 2.215 ± 0.399
0.574TyrHis: 0.574 ± 0.181
2.954TyrIle: 2.954 ± 0.547
3.364TyrLys: 3.364 ± 0.585
2.954TyrLeu: 2.954 ± 0.543
0.903TyrMet: 0.903 ± 0.291
1.805TyrAsn: 1.805 ± 0.392
0.656TyrPro: 0.656 ± 0.235
1.969TyrGln: 1.969 ± 0.423
1.723TyrArg: 1.723 ± 0.48
1.969TyrSer: 1.969 ± 0.421
1.559TyrThr: 1.559 ± 0.384
2.297TyrVal: 2.297 ± 0.37
0.328TyrTrp: 0.328 ± 0.154
1.313TyrTyr: 1.313 ± 0.375
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (12189 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski