Nocardioides silvaticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A316TQ38|A0A316TQ38_9ACTN DNA-protecting protein DprA OS=Nocardioides silvaticus OX=2201891 GN=dprA PE=3 SV=1
MM1 pKa = 7.21NRR3 pKa = 11.84TGRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84SATVGLWAALTVLAAIVFPSGAQAHH33 pKa = 7.27DD34 pKa = 3.98DD35 pKa = 3.58AGSRR39 pKa = 11.84GLSAGGTHH47 pKa = 6.4TCAIDD52 pKa = 3.42SAGSISCWGYY62 pKa = 11.21DD63 pKa = 4.64DD64 pKa = 5.48YY65 pKa = 11.99GQTTGPDD72 pKa = 3.17GAAGVFKK79 pKa = 10.58AVSAGSSHH87 pKa = 6.19TCAIDD92 pKa = 3.29ASGSISCWGEE102 pKa = 3.32NSYY105 pKa = 11.19GQVSGPDD112 pKa = 3.43AAAGTYY118 pKa = 8.15TAVSAGTSHH127 pKa = 6.47TCAIEE132 pKa = 3.91ATGSISCWGRR142 pKa = 11.84DD143 pKa = 3.36DD144 pKa = 4.43HH145 pKa = 6.87GQVGDD150 pKa = 3.94PDD152 pKa = 3.66AAAGTFKK159 pKa = 10.39TVAADD164 pKa = 3.75NLHH167 pKa = 5.71TCAVGTSGSISCWGDD182 pKa = 3.13DD183 pKa = 3.8SHH185 pKa = 7.04GQTSGPDD192 pKa = 3.34GAAGAFTTVSVRR204 pKa = 11.84YY205 pKa = 8.37FQTCAIDD212 pKa = 3.35VAGAISCWGNNGAGQVDD229 pKa = 4.19APIGAGFKK237 pKa = 9.56TVSLGLFHH245 pKa = 6.89TCAINAADD253 pKa = 6.0SIICWGHH260 pKa = 6.93DD261 pKa = 3.13MFGEE265 pKa = 4.05VDD267 pKa = 4.16GPNSSAGTFKK277 pKa = 10.47TLSLGDD283 pKa = 3.73GHH285 pKa = 6.54TCAIDD290 pKa = 3.45AADD293 pKa = 5.78SISCWGFDD301 pKa = 4.47SGIGWVTGADD311 pKa = 3.44AAPGSFGTTPADD323 pKa = 3.65PEE325 pKa = 4.42CTIGDD330 pKa = 3.88PNSNQRR336 pKa = 11.84QVLRR340 pKa = 11.84GTPGPDD346 pKa = 3.4VMCGGAAADD355 pKa = 4.01SFHH358 pKa = 6.78GAGGEE363 pKa = 4.06DD364 pKa = 3.87IILGGGGNDD373 pKa = 3.38WLNGGTGNDD382 pKa = 3.93TVDD385 pKa = 3.31GGAGQDD391 pKa = 3.51QLRR394 pKa = 11.84GEE396 pKa = 4.63AGADD400 pKa = 3.39ALDD403 pKa = 4.51GGDD406 pKa = 5.18DD407 pKa = 3.71NDD409 pKa = 4.16SVKK412 pKa = 11.04GGVGNDD418 pKa = 3.44TVTGGAGRR426 pKa = 11.84DD427 pKa = 3.68VLDD430 pKa = 3.54EE431 pKa = 4.14TGAAGFGNDD440 pKa = 4.97DD441 pKa = 4.63LDD443 pKa = 5.64GGDD446 pKa = 3.79GNDD449 pKa = 3.62VIRR452 pKa = 11.84GGEE455 pKa = 4.28GVDD458 pKa = 4.41SIAGGDD464 pKa = 3.97GNDD467 pKa = 3.96NLNGGAGSPDD477 pKa = 3.47VCDD480 pKa = 4.63GGPGTDD486 pKa = 2.71IGGYY490 pKa = 9.48GCEE493 pKa = 4.17QKK495 pKa = 11.03VSIPP499 pKa = 3.45

Molecular weight: 48.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A316TJC7|A0A316TJC7_9ACTN Respiratory nitrate reductase subunit gamma OS=Nocardioides silvaticus OX=2201891 GN=narI PE=4 SV=1
MM1 pKa = 7.66PGAAGPRR8 pKa = 11.84RR9 pKa = 11.84HH10 pKa = 5.87RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 5.98RR14 pKa = 11.84RR15 pKa = 11.84PHH17 pKa = 5.98RR18 pKa = 11.84RR19 pKa = 11.84PLLRR23 pKa = 11.84RR24 pKa = 11.84PPPRR28 pKa = 11.84QRR30 pKa = 11.84RR31 pKa = 11.84SRR33 pKa = 11.84RR34 pKa = 11.84TRR36 pKa = 11.84WPRR39 pKa = 11.84STPTSPPASSTRR51 pKa = 11.84RR52 pKa = 11.84AAGGPTTTRR61 pKa = 11.84PPPSRR66 pKa = 11.84SGTRR70 pKa = 11.84AATGSRR76 pKa = 11.84SRR78 pKa = 11.84AVPSCCRR85 pKa = 11.84TSSTRR90 pKa = 11.84SRR92 pKa = 11.84SWATRR97 pKa = 11.84PSSSRR102 pKa = 3.25

Molecular weight: 11.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4220

0

4220

1369492

33

2741

324.5

34.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.873 ± 0.055

0.747 ± 0.01

6.783 ± 0.033

6.123 ± 0.035

2.807 ± 0.021

9.177 ± 0.035

2.157 ± 0.018

3.552 ± 0.028

2.067 ± 0.029

10.091 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.758 ± 0.015

1.79 ± 0.022

5.668 ± 0.029

2.717 ± 0.019

7.628 ± 0.045

5.061 ± 0.028

6.025 ± 0.034

9.38 ± 0.041

1.566 ± 0.017

2.03 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski