Streptomyces phage Bowden

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Arequatrovirus; unclassified Arequatrovirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411CP07|A0A411CP07_9CAUD Minor tail protein OS=Streptomyces phage Bowden OX=2510524 GN=21 PE=4 SV=1
MM1 pKa = 7.75ALNDD5 pKa = 3.72AATLVIGSGNYY16 pKa = 7.79LTAPVGTDD24 pKa = 2.71IPTDD28 pKa = 3.63LLVPVSPWEE37 pKa = 4.27AVGHH41 pKa = 5.85TSLEE45 pKa = 4.62DD46 pKa = 3.09IFSIASEE53 pKa = 4.21GGEE56 pKa = 3.82ATVIGSLQNKK66 pKa = 7.32SLRR69 pKa = 11.84TKK71 pKa = 10.19YY72 pKa = 10.01SARR75 pKa = 11.84TEE77 pKa = 4.14TMAFTLQQFDD87 pKa = 3.92VKK89 pKa = 10.73GLKK92 pKa = 9.98LYY94 pKa = 10.4YY95 pKa = 10.42GSNAPILPDD104 pKa = 3.49GSVGVPADD112 pKa = 3.93PVPTTAAFLAVFVDD126 pKa = 4.2GEE128 pKa = 4.59NVFAFYY134 pKa = 10.79APKK137 pKa = 10.81AEE139 pKa = 4.35IYY141 pKa = 10.76RR142 pKa = 11.84NDD144 pKa = 3.94DD145 pKa = 3.38LSFGDD150 pKa = 3.96TEE152 pKa = 4.6SLAGLPIGVKK162 pKa = 10.18PMTYY166 pKa = 10.33GANSWTYY173 pKa = 11.09AITPLGTVSATGATAGTPGSFTPAGSTAPYY203 pKa = 10.82DD204 pKa = 4.58LVDD207 pKa = 4.94LDD209 pKa = 5.83LVTADD214 pKa = 4.91PATAWTTGQYY224 pKa = 10.36VVLGDD229 pKa = 4.16GSNASWNGTDD239 pKa = 3.04WVEE242 pKa = 4.94GIAAA246 pKa = 4.47

Molecular weight: 25.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411CPP8|A0A411CPP8_9CAUD Tail terminator OS=Streptomyces phage Bowden OX=2510524 GN=15 PE=4 SV=1
MM1 pKa = 7.05NAKK4 pKa = 9.22EE5 pKa = 4.06LRR7 pKa = 11.84EE8 pKa = 4.02AQQAGRR14 pKa = 11.84RR15 pKa = 11.84IEE17 pKa = 4.4GAVPPAVGRR26 pKa = 11.84EE27 pKa = 3.76MEE29 pKa = 4.0YY30 pKa = 10.49RR31 pKa = 11.84PRR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 8.22TDD37 pKa = 3.25ALPWIEE43 pKa = 4.56KK44 pKa = 10.04GQVHH48 pKa = 6.68DD49 pKa = 3.53WARR52 pKa = 11.84YY53 pKa = 7.39RR54 pKa = 11.84SRR56 pKa = 11.84EE57 pKa = 3.67VRR59 pKa = 11.84AAA61 pKa = 3.28

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

15645

34

1086

193.1

21.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.473 ± 0.368

0.697 ± 0.124

6.494 ± 0.251

6.954 ± 0.395

3.036 ± 0.193

8.392 ± 0.301

2.128 ± 0.208

4.072 ± 0.278

4.551 ± 0.263

8.418 ± 0.355

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.097 ± 0.131

2.787 ± 0.14

4.756 ± 0.196

3.343 ± 0.149

6.411 ± 0.354

5.797 ± 0.275

6.462 ± 0.279

7.389 ± 0.269

1.93 ± 0.123

2.812 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski