Amino acid dipepetide frequency for Streptomyces phage Bowden

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.721AlaAla: 12.721 ± 1.086
0.511AlaCys: 0.511 ± 0.178
7.031AlaAsp: 7.031 ± 0.627
8.821AlaGlu: 8.821 ± 0.796
2.813AlaPhe: 2.813 ± 0.455
9.844AlaGly: 9.844 ± 0.812
2.046AlaHis: 2.046 ± 0.391
4.794AlaIle: 4.794 ± 0.597
5.753AlaLys: 5.753 ± 0.624
11.25AlaLeu: 11.25 ± 0.987
2.365AlaMet: 2.365 ± 0.325
2.493AlaAsn: 2.493 ± 0.327
5.369AlaPro: 5.369 ± 0.478
4.155AlaGln: 4.155 ± 0.609
7.223AlaArg: 7.223 ± 0.761
5.881AlaSer: 5.881 ± 0.535
6.968AlaThr: 6.968 ± 0.741
9.205AlaVal: 9.205 ± 0.775
2.301AlaTrp: 2.301 ± 0.365
3.132AlaTyr: 3.132 ± 0.485
0.0AlaXaa: 0.0 ± 0.0
Cys
0.511CysAla: 0.511 ± 0.212
0.064CysCys: 0.064 ± 0.061
0.703CysAsp: 0.703 ± 0.205
0.192CysGlu: 0.192 ± 0.112
0.256CysPhe: 0.256 ± 0.148
0.575CysGly: 0.575 ± 0.163
0.32CysHis: 0.32 ± 0.111
0.447CysIle: 0.447 ± 0.154
0.447CysLys: 0.447 ± 0.172
0.256CysLeu: 0.256 ± 0.126
0.064CysMet: 0.064 ± 0.071
0.192CysAsn: 0.192 ± 0.096
0.447CysPro: 0.447 ± 0.184
0.064CysGln: 0.064 ± 0.065
0.256CysArg: 0.256 ± 0.105
0.767CysSer: 0.767 ± 0.205
0.639CysThr: 0.639 ± 0.211
0.192CysVal: 0.192 ± 0.12
0.32CysTrp: 0.32 ± 0.162
0.256CysTyr: 0.256 ± 0.118
0.0CysXaa: 0.0 ± 0.0
Asp
7.031AspAla: 7.031 ± 0.653
0.575AspCys: 0.575 ± 0.245
3.963AspAsp: 3.963 ± 0.567
5.369AspGlu: 5.369 ± 0.798
2.109AspPhe: 2.109 ± 0.388
6.584AspGly: 6.584 ± 0.803
1.47AspHis: 1.47 ± 0.337
2.557AspIle: 2.557 ± 0.47
2.237AspLys: 2.237 ± 0.419
5.369AspLeu: 5.369 ± 0.566
1.918AspMet: 1.918 ± 0.322
1.534AspAsn: 1.534 ± 0.308
4.347AspPro: 4.347 ± 0.484
1.982AspGln: 1.982 ± 0.348
3.452AspArg: 3.452 ± 0.45
4.027AspSer: 4.027 ± 0.555
3.132AspThr: 3.132 ± 0.513
4.091AspVal: 4.091 ± 0.411
1.854AspTrp: 1.854 ± 0.327
1.342AspTyr: 1.342 ± 0.279
0.0AspXaa: 0.0 ± 0.0
Glu
8.502GluAla: 8.502 ± 0.872
0.639GluCys: 0.639 ± 0.187
4.347GluAsp: 4.347 ± 0.632
5.625GluGlu: 5.625 ± 0.781
1.854GluPhe: 1.854 ± 0.318
6.2GluGly: 6.2 ± 0.583
1.598GluHis: 1.598 ± 0.341
3.068GluIle: 3.068 ± 0.532
2.493GluLys: 2.493 ± 0.386
8.182GluLeu: 8.182 ± 0.856
1.278GluMet: 1.278 ± 0.26
1.087GluAsn: 1.087 ± 0.227
2.365GluPro: 2.365 ± 0.428
2.877GluGln: 2.877 ± 0.391
3.899GluArg: 3.899 ± 0.575
3.132GluSer: 3.132 ± 0.511
3.771GluThr: 3.771 ± 0.575
5.433GluVal: 5.433 ± 0.585
1.278GluTrp: 1.278 ± 0.265
1.918GluTyr: 1.918 ± 0.363
0.0GluXaa: 0.0 ± 0.0
Phe
3.26PheAla: 3.26 ± 0.478
0.32PheCys: 0.32 ± 0.141
2.046PheAsp: 2.046 ± 0.316
2.046PheGlu: 2.046 ± 0.327
0.767PhePhe: 0.767 ± 0.257
3.26PheGly: 3.26 ± 0.38
0.511PheHis: 0.511 ± 0.179
0.895PheIle: 0.895 ± 0.216
1.662PheLys: 1.662 ± 0.322
1.79PheLeu: 1.79 ± 0.3
0.703PheMet: 0.703 ± 0.236
1.023PheAsn: 1.023 ± 0.213
1.47PhePro: 1.47 ± 0.401
0.959PheGln: 0.959 ± 0.227
2.173PheArg: 2.173 ± 0.43
1.918PheSer: 1.918 ± 0.351
1.982PheThr: 1.982 ± 0.388
2.046PheVal: 2.046 ± 0.296
0.575PheTrp: 0.575 ± 0.159
0.959PheTyr: 0.959 ± 0.251
0.0PheXaa: 0.0 ± 0.0
Gly
7.799GlyAla: 7.799 ± 0.813
0.447GlyCys: 0.447 ± 0.169
5.306GlyAsp: 5.306 ± 0.671
4.922GlyGlu: 4.922 ± 0.528
3.068GlyPhe: 3.068 ± 0.408
6.073GlyGly: 6.073 ± 0.926
2.429GlyHis: 2.429 ± 0.456
3.388GlyIle: 3.388 ± 0.629
4.986GlyLys: 4.986 ± 0.718
6.712GlyLeu: 6.712 ± 0.834
1.726GlyMet: 1.726 ± 0.28
2.877GlyAsn: 2.877 ± 0.406
3.58GlyPro: 3.58 ± 0.523
2.94GlyGln: 2.94 ± 0.436
5.242GlyArg: 5.242 ± 0.636
5.369GlySer: 5.369 ± 0.944
5.497GlyThr: 5.497 ± 0.827
6.2GlyVal: 6.2 ± 0.618
2.429GlyTrp: 2.429 ± 0.414
2.94GlyTyr: 2.94 ± 0.549
0.0GlyXaa: 0.0 ± 0.0
His
2.046HisAla: 2.046 ± 0.361
0.256HisCys: 0.256 ± 0.127
1.406HisAsp: 1.406 ± 0.242
1.534HisGlu: 1.534 ± 0.338
0.895HisPhe: 0.895 ± 0.296
1.47HisGly: 1.47 ± 0.309
0.767HisHis: 0.767 ± 0.214
0.703HisIle: 0.703 ± 0.204
0.447HisLys: 0.447 ± 0.171
2.046HisLeu: 2.046 ± 0.407
0.256HisMet: 0.256 ± 0.132
0.511HisAsn: 0.511 ± 0.191
1.342HisPro: 1.342 ± 0.263
0.895HisGln: 0.895 ± 0.236
1.47HisArg: 1.47 ± 0.343
1.215HisSer: 1.215 ± 0.357
1.215HisThr: 1.215 ± 0.238
1.726HisVal: 1.726 ± 0.451
0.703HisTrp: 0.703 ± 0.201
0.384HisTyr: 0.384 ± 0.158
0.0HisXaa: 0.0 ± 0.0
Ile
4.347IleAla: 4.347 ± 0.449
0.128IleCys: 0.128 ± 0.085
3.26IleAsp: 3.26 ± 0.498
3.58IleGlu: 3.58 ± 0.482
1.215IlePhe: 1.215 ± 0.297
3.452IleGly: 3.452 ± 0.618
0.575IleHis: 0.575 ± 0.171
1.598IleIle: 1.598 ± 0.426
1.662IleLys: 1.662 ± 0.309
3.132IleLeu: 3.132 ± 0.583
0.639IleMet: 0.639 ± 0.242
1.023IleAsn: 1.023 ± 0.311
2.046IlePro: 2.046 ± 0.359
1.215IleGln: 1.215 ± 0.275
3.004IleArg: 3.004 ± 0.397
1.918IleSer: 1.918 ± 0.333
3.26IleThr: 3.26 ± 0.374
3.196IleVal: 3.196 ± 0.443
0.32IleTrp: 0.32 ± 0.121
1.151IleTyr: 1.151 ± 0.24
0.0IleXaa: 0.0 ± 0.0
Lys
4.986LysAla: 4.986 ± 0.611
0.256LysCys: 0.256 ± 0.105
2.365LysAsp: 2.365 ± 0.493
2.877LysGlu: 2.877 ± 0.411
1.023LysPhe: 1.023 ± 0.188
4.602LysGly: 4.602 ± 0.571
1.087LysHis: 1.087 ± 0.267
1.662LysIle: 1.662 ± 0.318
2.685LysLys: 2.685 ± 0.468
4.219LysLeu: 4.219 ± 0.565
0.895LysMet: 0.895 ± 0.218
1.278LysAsn: 1.278 ± 0.285
2.493LysPro: 2.493 ± 0.475
1.406LysGln: 1.406 ± 0.252
3.26LysArg: 3.26 ± 0.581
2.685LysSer: 2.685 ± 0.509
3.132LysThr: 3.132 ± 0.412
2.685LysVal: 2.685 ± 0.421
0.447LysTrp: 0.447 ± 0.149
1.47LysTyr: 1.47 ± 0.316
0.0LysXaa: 0.0 ± 0.0
Leu
11.89LeuAla: 11.89 ± 0.967
0.447LeuCys: 0.447 ± 0.143
5.625LeuAsp: 5.625 ± 0.483
4.538LeuGlu: 4.538 ± 0.527
2.109LeuPhe: 2.109 ± 0.341
6.904LeuGly: 6.904 ± 0.698
1.278LeuHis: 1.278 ± 0.288
3.771LeuIle: 3.771 ± 0.461
3.068LeuLys: 3.068 ± 0.458
6.776LeuLeu: 6.776 ± 0.762
1.726LeuMet: 1.726 ± 0.285
3.196LeuAsn: 3.196 ± 0.422
4.411LeuPro: 4.411 ± 0.609
3.388LeuGln: 3.388 ± 0.394
5.689LeuArg: 5.689 ± 0.617
4.794LeuSer: 4.794 ± 0.5
5.561LeuThr: 5.561 ± 0.505
6.584LeuVal: 6.584 ± 0.828
0.895LeuTrp: 0.895 ± 0.254
1.534LeuTyr: 1.534 ± 0.288
0.0LeuXaa: 0.0 ± 0.0
Met
3.516MetAla: 3.516 ± 0.558
0.128MetCys: 0.128 ± 0.089
1.342MetAsp: 1.342 ± 0.267
0.959MetGlu: 0.959 ± 0.262
0.384MetPhe: 0.384 ± 0.137
1.023MetGly: 1.023 ± 0.232
0.511MetHis: 0.511 ± 0.169
0.767MetIle: 0.767 ± 0.209
0.639MetLys: 0.639 ± 0.189
1.854MetLeu: 1.854 ± 0.374
0.256MetMet: 0.256 ± 0.112
0.575MetAsn: 0.575 ± 0.179
1.023MetPro: 1.023 ± 0.199
0.511MetGln: 0.511 ± 0.178
1.151MetArg: 1.151 ± 0.315
2.621MetSer: 2.621 ± 0.363
1.662MetThr: 1.662 ± 0.329
1.215MetVal: 1.215 ± 0.279
0.447MetTrp: 0.447 ± 0.128
0.384MetTyr: 0.384 ± 0.145
0.0MetXaa: 0.0 ± 0.0
Asn
3.196AsnAla: 3.196 ± 0.357
0.447AsnCys: 0.447 ± 0.159
1.534AsnAsp: 1.534 ± 0.256
1.79AsnGlu: 1.79 ± 0.312
0.959AsnPhe: 0.959 ± 0.193
2.94AsnGly: 2.94 ± 0.678
0.703AsnHis: 0.703 ± 0.227
1.151AsnIle: 1.151 ± 0.254
1.087AsnLys: 1.087 ± 0.219
2.109AsnLeu: 2.109 ± 0.423
0.447AsnMet: 0.447 ± 0.151
0.959AsnAsn: 0.959 ± 0.231
1.726AsnPro: 1.726 ± 0.33
0.831AsnGln: 0.831 ± 0.219
1.47AsnArg: 1.47 ± 0.257
1.726AsnSer: 1.726 ± 0.362
1.342AsnThr: 1.342 ± 0.302
2.173AsnVal: 2.173 ± 0.302
0.767AsnTrp: 0.767 ± 0.204
0.511AsnTyr: 0.511 ± 0.14
0.0AsnXaa: 0.0 ± 0.0
Pro
5.881ProAla: 5.881 ± 0.659
0.511ProCys: 0.511 ± 0.159
3.644ProAsp: 3.644 ± 0.528
3.835ProGlu: 3.835 ± 0.453
1.47ProPhe: 1.47 ± 0.291
4.411ProGly: 4.411 ± 0.507
0.447ProHis: 0.447 ± 0.166
1.918ProIle: 1.918 ± 0.442
2.94ProLys: 2.94 ± 0.599
2.94ProLeu: 2.94 ± 0.496
1.023ProMet: 1.023 ± 0.229
1.087ProAsn: 1.087 ± 0.317
2.173ProPro: 2.173 ± 0.371
1.47ProGln: 1.47 ± 0.267
1.918ProArg: 1.918 ± 0.332
2.749ProSer: 2.749 ± 0.553
3.132ProThr: 3.132 ± 0.509
3.835ProVal: 3.835 ± 0.392
0.831ProTrp: 0.831 ± 0.225
1.342ProTyr: 1.342 ± 0.352
0.0ProXaa: 0.0 ± 0.0
Gln
4.986GlnAla: 4.986 ± 0.489
0.32GlnCys: 0.32 ± 0.121
1.598GlnAsp: 1.598 ± 0.33
2.365GlnGlu: 2.365 ± 0.431
0.895GlnPhe: 0.895 ± 0.261
2.237GlnGly: 2.237 ± 0.376
0.575GlnHis: 0.575 ± 0.185
1.726GlnIle: 1.726 ± 0.308
1.79GlnLys: 1.79 ± 0.377
3.068GlnLeu: 3.068 ± 0.429
0.895GlnMet: 0.895 ± 0.289
0.831GlnAsn: 0.831 ± 0.251
1.087GlnPro: 1.087 ± 0.216
1.151GlnGln: 1.151 ± 0.264
2.493GlnArg: 2.493 ± 0.364
1.534GlnSer: 1.534 ± 0.34
1.854GlnThr: 1.854 ± 0.444
2.621GlnVal: 2.621 ± 0.451
0.575GlnTrp: 0.575 ± 0.163
0.831GlnTyr: 0.831 ± 0.245
0.0GlnXaa: 0.0 ± 0.0
Arg
5.753ArgAla: 5.753 ± 0.626
0.575ArgCys: 0.575 ± 0.182
4.027ArgAsp: 4.027 ± 0.523
4.922ArgGlu: 4.922 ± 0.697
2.557ArgPhe: 2.557 ± 0.316
3.899ArgGly: 3.899 ± 0.586
1.534ArgHis: 1.534 ± 0.334
2.557ArgIle: 2.557 ± 0.357
3.899ArgLys: 3.899 ± 0.505
4.922ArgLeu: 4.922 ± 0.589
1.726ArgMet: 1.726 ± 0.329
1.982ArgAsn: 1.982 ± 0.331
2.877ArgPro: 2.877 ± 0.386
2.429ArgGln: 2.429 ± 0.51
5.625ArgArg: 5.625 ± 0.839
3.388ArgSer: 3.388 ± 0.49
3.835ArgThr: 3.835 ± 0.483
4.475ArgVal: 4.475 ± 0.54
0.959ArgTrp: 0.959 ± 0.323
2.173ArgTyr: 2.173 ± 0.264
0.0ArgXaa: 0.0 ± 0.0
Ser
6.392SerAla: 6.392 ± 0.66
0.064SerCys: 0.064 ± 0.062
3.707SerAsp: 3.707 ± 0.506
3.516SerGlu: 3.516 ± 0.46
1.726SerPhe: 1.726 ± 0.385
5.05SerGly: 5.05 ± 0.621
1.534SerHis: 1.534 ± 0.477
2.813SerIle: 2.813 ± 0.408
2.429SerLys: 2.429 ± 0.369
5.114SerLeu: 5.114 ± 0.628
1.023SerMet: 1.023 ± 0.269
1.598SerAsn: 1.598 ± 0.387
2.429SerPro: 2.429 ± 0.339
1.598SerGln: 1.598 ± 0.42
3.516SerArg: 3.516 ± 0.627
3.516SerSer: 3.516 ± 0.686
4.411SerThr: 4.411 ± 0.517
4.219SerVal: 4.219 ± 0.535
1.215SerTrp: 1.215 ± 0.262
2.109SerTyr: 2.109 ± 0.408
0.0SerXaa: 0.0 ± 0.0
Thr
7.031ThrAla: 7.031 ± 0.626
0.384ThrCys: 0.384 ± 0.162
4.347ThrAsp: 4.347 ± 0.504
4.219ThrGlu: 4.219 ± 0.553
2.621ThrPhe: 2.621 ± 0.429
5.625ThrGly: 5.625 ± 0.705
0.895ThrHis: 0.895 ± 0.236
2.493ThrIle: 2.493 ± 0.532
1.79ThrLys: 1.79 ± 0.237
5.689ThrLeu: 5.689 ± 0.634
0.959ThrMet: 0.959 ± 0.25
1.598ThrAsn: 1.598 ± 0.374
3.388ThrPro: 3.388 ± 0.495
1.79ThrGln: 1.79 ± 0.403
3.771ThrArg: 3.771 ± 0.504
3.771ThrSer: 3.771 ± 0.695
4.411ThrThr: 4.411 ± 0.663
5.945ThrVal: 5.945 ± 0.569
1.151ThrTrp: 1.151 ± 0.285
2.749ThrTyr: 2.749 ± 0.381
0.0ThrXaa: 0.0 ± 0.0
Val
8.949ValAla: 8.949 ± 0.682
0.511ValCys: 0.511 ± 0.192
4.538ValAsp: 4.538 ± 0.402
4.73ValGlu: 4.73 ± 0.57
2.046ValPhe: 2.046 ± 0.314
5.497ValGly: 5.497 ± 0.727
1.982ValHis: 1.982 ± 0.348
3.26ValIle: 3.26 ± 0.376
3.452ValLys: 3.452 ± 0.409
5.625ValLeu: 5.625 ± 0.587
1.854ValMet: 1.854 ± 0.301
2.557ValAsn: 2.557 ± 0.523
3.004ValPro: 3.004 ± 0.437
2.877ValGln: 2.877 ± 0.419
4.794ValArg: 4.794 ± 0.521
4.155ValSer: 4.155 ± 0.504
5.561ValThr: 5.561 ± 0.612
5.178ValVal: 5.178 ± 0.688
1.534ValTrp: 1.534 ± 0.265
1.79ValTyr: 1.79 ± 0.378
0.0ValXaa: 0.0 ± 0.0
Trp
2.493TrpAla: 2.493 ± 0.372
0.32TrpCys: 0.32 ± 0.135
1.47TrpAsp: 1.47 ± 0.283
1.342TrpGlu: 1.342 ± 0.26
0.639TrpPhe: 0.639 ± 0.2
1.278TrpGly: 1.278 ± 0.28
0.447TrpHis: 0.447 ± 0.171
0.384TrpIle: 0.384 ± 0.148
1.278TrpLys: 1.278 ± 0.252
1.023TrpLeu: 1.023 ± 0.243
0.639TrpMet: 0.639 ± 0.176
0.511TrpAsn: 0.511 ± 0.179
0.767TrpPro: 0.767 ± 0.188
0.256TrpGln: 0.256 ± 0.142
1.534TrpArg: 1.534 ± 0.342
1.087TrpSer: 1.087 ± 0.257
1.918TrpThr: 1.918 ± 0.351
1.342TrpVal: 1.342 ± 0.286
0.128TrpTrp: 0.128 ± 0.141
0.447TrpTyr: 0.447 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.452TyrAla: 3.452 ± 0.394
0.064TyrCys: 0.064 ± 0.062
2.685TyrAsp: 2.685 ± 0.359
2.365TyrGlu: 2.365 ± 0.299
0.959TyrPhe: 0.959 ± 0.202
3.004TyrGly: 3.004 ± 0.578
0.575TyrHis: 0.575 ± 0.218
0.767TyrIle: 0.767 ± 0.218
0.767TyrLys: 0.767 ± 0.221
2.109TyrLeu: 2.109 ± 0.336
0.511TyrMet: 0.511 ± 0.155
0.959TyrAsn: 0.959 ± 0.261
1.215TyrPro: 1.215 ± 0.397
0.639TyrGln: 0.639 ± 0.193
2.173TyrArg: 2.173 ± 0.422
1.726TyrSer: 1.726 ± 0.321
1.342TyrThr: 1.342 ± 0.3
1.534TyrVal: 1.534 ± 0.253
0.575TyrTrp: 0.575 ± 0.204
0.703TyrTyr: 0.703 ± 0.208
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 81 proteins (15645 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski