Amino acid dipepetide frequency for Bacillus phage 019DV002

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.302AlaAla: 3.302 ± 0.941
0.561AlaCys: 0.561 ± 0.226
4.112AlaAsp: 4.112 ± 0.513
4.611AlaGlu: 4.611 ± 0.525
3.24AlaPhe: 3.24 ± 0.453
4.86AlaGly: 4.86 ± 0.699
1.308AlaHis: 1.308 ± 0.255
4.549AlaIle: 4.549 ± 0.612
4.922AlaLys: 4.922 ± 0.515
6.667AlaLeu: 6.667 ± 0.753
1.184AlaMet: 1.184 ± 0.212
3.053AlaAsn: 3.053 ± 0.439
2.43AlaPro: 2.43 ± 0.342
2.555AlaGln: 2.555 ± 0.373
3.24AlaArg: 3.24 ± 0.453
4.05AlaSer: 4.05 ± 0.64
3.614AlaThr: 3.614 ± 0.442
4.611AlaVal: 4.611 ± 0.689
1.184AlaTrp: 1.184 ± 0.349
2.804AlaTyr: 2.804 ± 0.433
0.0AlaXaa: 0.0 ± 0.0
Cys
0.312CysAla: 0.312 ± 0.132
0.0CysCys: 0.0 ± 0.0
0.374CysAsp: 0.374 ± 0.139
0.561CysGlu: 0.561 ± 0.202
0.685CysPhe: 0.685 ± 0.215
0.685CysGly: 0.685 ± 0.261
0.436CysHis: 0.436 ± 0.156
0.436CysIle: 0.436 ± 0.165
0.997CysLys: 0.997 ± 0.303
0.872CysLeu: 0.872 ± 0.252
0.312CysMet: 0.312 ± 0.176
0.249CysAsn: 0.249 ± 0.18
0.498CysPro: 0.498 ± 0.217
0.561CysGln: 0.561 ± 0.169
0.249CysArg: 0.249 ± 0.137
0.685CysSer: 0.685 ± 0.273
0.685CysThr: 0.685 ± 0.204
0.249CysVal: 0.249 ± 0.124
0.062CysTrp: 0.062 ± 0.07
0.249CysTyr: 0.249 ± 0.162
0.0CysXaa: 0.0 ± 0.0
Asp
3.552AspAla: 3.552 ± 0.518
0.685AspCys: 0.685 ± 0.271
2.617AspAsp: 2.617 ± 0.401
4.362AspGlu: 4.362 ± 0.71
2.929AspPhe: 2.929 ± 0.504
4.05AspGly: 4.05 ± 0.535
0.935AspHis: 0.935 ± 0.252
4.05AspIle: 4.05 ± 0.467
4.486AspLys: 4.486 ± 0.695
5.109AspLeu: 5.109 ± 0.526
1.745AspMet: 1.745 ± 0.302
2.617AspAsn: 2.617 ± 0.343
1.62AspPro: 1.62 ± 0.382
1.682AspGln: 1.682 ± 0.268
2.742AspArg: 2.742 ± 0.382
3.302AspSer: 3.302 ± 0.462
3.676AspThr: 3.676 ± 0.508
3.053AspVal: 3.053 ± 0.403
1.246AspTrp: 1.246 ± 0.276
3.053AspTyr: 3.053 ± 0.607
0.0AspXaa: 0.0 ± 0.0
Glu
5.421GluAla: 5.421 ± 0.632
0.498GluCys: 0.498 ± 0.172
4.299GluAsp: 4.299 ± 0.59
8.474GluGlu: 8.474 ± 1.203
3.863GluPhe: 3.863 ± 0.5
5.857GluGly: 5.857 ± 0.63
1.745GluHis: 1.745 ± 0.414
4.486GluIle: 4.486 ± 0.371
7.726GluLys: 7.726 ± 0.758
7.913GluLeu: 7.913 ± 0.828
2.181GluMet: 2.181 ± 0.465
3.365GluAsn: 3.365 ± 0.579
1.807GluPro: 1.807 ± 0.43
4.486GluGln: 4.486 ± 0.622
3.302GluArg: 3.302 ± 0.522
3.801GluSer: 3.801 ± 0.498
3.676GluThr: 3.676 ± 0.487
5.483GluVal: 5.483 ± 0.662
1.433GluTrp: 1.433 ± 0.306
3.552GluTyr: 3.552 ± 0.361
0.0GluXaa: 0.0 ± 0.0
Phe
2.679PheAla: 2.679 ± 0.336
0.187PheCys: 0.187 ± 0.125
3.24PheAsp: 3.24 ± 0.453
3.988PheGlu: 3.988 ± 0.543
1.433PhePhe: 1.433 ± 0.273
2.804PheGly: 2.804 ± 0.3
0.623PheHis: 0.623 ± 0.179
1.994PheIle: 1.994 ± 0.352
2.617PheLys: 2.617 ± 0.379
3.365PheLeu: 3.365 ± 0.492
1.62PheMet: 1.62 ± 0.354
2.305PheAsn: 2.305 ± 0.334
1.371PhePro: 1.371 ± 0.32
1.495PheGln: 1.495 ± 0.308
1.558PheArg: 1.558 ± 0.338
2.742PheSer: 2.742 ± 0.519
3.552PheThr: 3.552 ± 0.622
2.866PheVal: 2.866 ± 0.448
0.312PheTrp: 0.312 ± 0.161
1.558PheTyr: 1.558 ± 0.314
0.0PheXaa: 0.0 ± 0.0
Gly
4.486GlyAla: 4.486 ± 0.652
0.81GlyCys: 0.81 ± 0.212
3.614GlyAsp: 3.614 ± 0.466
5.421GlyGlu: 5.421 ± 0.549
3.614GlyPhe: 3.614 ± 0.428
4.798GlyGly: 4.798 ± 0.544
1.184GlyHis: 1.184 ± 0.277
4.922GlyIle: 4.922 ± 0.867
5.67GlyLys: 5.67 ± 0.46
4.112GlyLeu: 4.112 ± 0.501
2.305GlyMet: 2.305 ± 0.373
2.866GlyAsn: 2.866 ± 0.684
0.0GlyPro: 0.0 ± 0.0
1.994GlyGln: 1.994 ± 0.419
2.555GlyArg: 2.555 ± 0.346
5.421GlySer: 5.421 ± 0.558
3.614GlyThr: 3.614 ± 0.599
5.109GlyVal: 5.109 ± 0.564
1.122GlyTrp: 1.122 ± 0.287
3.801GlyTyr: 3.801 ± 0.541
0.0GlyXaa: 0.0 ± 0.0
His
0.623HisAla: 0.623 ± 0.193
0.187HisCys: 0.187 ± 0.105
1.122HisAsp: 1.122 ± 0.234
1.495HisGlu: 1.495 ± 0.254
0.623HisPhe: 0.623 ± 0.181
1.807HisGly: 1.807 ± 0.373
0.312HisHis: 0.312 ± 0.131
0.997HisIle: 0.997 ± 0.246
1.184HisLys: 1.184 ± 0.277
1.371HisLeu: 1.371 ± 0.322
0.374HisMet: 0.374 ± 0.146
0.685HisAsn: 0.685 ± 0.186
0.935HisPro: 0.935 ± 0.22
0.062HisGln: 0.062 ± 0.05
0.685HisArg: 0.685 ± 0.162
1.122HisSer: 1.122 ± 0.369
1.246HisThr: 1.246 ± 0.246
0.935HisVal: 0.935 ± 0.267
0.249HisTrp: 0.249 ± 0.1
0.872HisTyr: 0.872 ± 0.226
0.0HisXaa: 0.0 ± 0.0
Ile
4.86IleAla: 4.86 ± 0.559
0.498IleCys: 0.498 ± 0.203
5.359IleAsp: 5.359 ± 0.507
6.293IleGlu: 6.293 ± 0.725
2.119IlePhe: 2.119 ± 0.352
3.115IleGly: 3.115 ± 0.501
1.122IleHis: 1.122 ± 0.299
3.925IleIle: 3.925 ± 0.73
5.359IleLys: 5.359 ± 0.604
4.05IleLeu: 4.05 ± 0.583
1.869IleMet: 1.869 ± 0.304
3.365IleAsn: 3.365 ± 0.512
2.243IlePro: 2.243 ± 0.379
2.866IleGln: 2.866 ± 0.502
2.617IleArg: 2.617 ± 0.422
5.109IleSer: 5.109 ± 0.624
4.735IleThr: 4.735 ± 0.643
3.427IleVal: 3.427 ± 0.462
0.561IleTrp: 0.561 ± 0.199
2.119IleTyr: 2.119 ± 0.425
0.0IleXaa: 0.0 ± 0.0
Lys
5.795LysAla: 5.795 ± 0.779
0.312LysCys: 0.312 ± 0.141
3.676LysAsp: 3.676 ± 0.6
6.979LysGlu: 6.979 ± 0.735
3.614LysPhe: 3.614 ± 0.587
5.982LysGly: 5.982 ± 0.679
1.495LysHis: 1.495 ± 0.309
5.296LysIle: 5.296 ± 0.597
6.854LysLys: 6.854 ± 0.841
7.726LysLeu: 7.726 ± 0.717
2.929LysMet: 2.929 ± 0.488
3.365LysAsn: 3.365 ± 0.54
2.368LysPro: 2.368 ± 0.348
2.555LysGln: 2.555 ± 0.473
4.549LysArg: 4.549 ± 0.486
4.05LysSer: 4.05 ± 0.47
3.739LysThr: 3.739 ± 0.487
4.798LysVal: 4.798 ± 0.528
0.561LysTrp: 0.561 ± 0.181
2.866LysTyr: 2.866 ± 0.375
0.0LysXaa: 0.0 ± 0.0
Leu
5.608LeuAla: 5.608 ± 0.528
0.685LeuCys: 0.685 ± 0.194
4.611LeuAsp: 4.611 ± 0.569
6.979LeuGlu: 6.979 ± 0.656
2.43LeuPhe: 2.43 ± 0.399
5.67LeuGly: 5.67 ± 0.547
1.682LeuHis: 1.682 ± 0.362
5.608LeuIle: 5.608 ± 0.744
6.293LeuLys: 6.293 ± 0.768
5.483LeuLeu: 5.483 ± 0.819
1.932LeuMet: 1.932 ± 0.308
4.424LeuAsn: 4.424 ± 0.581
3.365LeuPro: 3.365 ± 0.583
3.115LeuGln: 3.115 ± 0.404
3.614LeuArg: 3.614 ± 0.416
5.421LeuSer: 5.421 ± 0.637
4.735LeuThr: 4.735 ± 0.678
4.549LeuVal: 4.549 ± 0.675
1.246LeuTrp: 1.246 ± 0.246
3.302LeuTyr: 3.302 ± 0.433
0.0LeuXaa: 0.0 ± 0.0
Met
2.119MetAla: 2.119 ± 0.475
0.374MetCys: 0.374 ± 0.202
1.308MetAsp: 1.308 ± 0.232
1.682MetGlu: 1.682 ± 0.365
1.371MetPhe: 1.371 ± 0.35
1.558MetGly: 1.558 ± 0.305
0.498MetHis: 0.498 ± 0.216
2.305MetIle: 2.305 ± 0.44
2.617MetLys: 2.617 ± 0.466
1.869MetLeu: 1.869 ± 0.366
0.748MetMet: 0.748 ± 0.223
1.558MetAsn: 1.558 ± 0.263
0.997MetPro: 0.997 ± 0.266
0.748MetGln: 0.748 ± 0.206
1.495MetArg: 1.495 ± 0.289
1.994MetSer: 1.994 ± 0.354
2.181MetThr: 2.181 ± 0.414
1.308MetVal: 1.308 ± 0.334
0.187MetTrp: 0.187 ± 0.135
1.308MetTyr: 1.308 ± 0.384
0.0MetXaa: 0.0 ± 0.0
Asn
3.489AsnAla: 3.489 ± 0.411
0.685AsnCys: 0.685 ± 0.218
2.742AsnAsp: 2.742 ± 0.514
3.801AsnGlu: 3.801 ± 0.49
2.305AsnPhe: 2.305 ± 0.352
3.552AsnGly: 3.552 ± 0.489
0.498AsnHis: 0.498 ± 0.185
2.368AsnIle: 2.368 ± 0.428
3.925AsnLys: 3.925 ± 0.488
3.739AsnLeu: 3.739 ± 0.533
1.433AsnMet: 1.433 ± 0.26
3.178AsnAsn: 3.178 ± 0.551
1.932AsnPro: 1.932 ± 0.375
1.682AsnGln: 1.682 ± 0.237
2.492AsnArg: 2.492 ± 0.377
2.866AsnSer: 2.866 ± 0.461
3.115AsnThr: 3.115 ± 0.549
2.742AsnVal: 2.742 ± 0.465
0.561AsnTrp: 0.561 ± 0.16
1.807AsnTyr: 1.807 ± 0.388
0.0AsnXaa: 0.0 ± 0.0
Pro
2.492ProAla: 2.492 ± 0.465
0.062ProCys: 0.062 ± 0.063
1.932ProAsp: 1.932 ± 0.332
3.614ProGlu: 3.614 ± 0.519
1.184ProPhe: 1.184 ± 0.307
0.062ProGly: 0.062 ± 0.05
0.997ProHis: 0.997 ± 0.249
1.807ProIle: 1.807 ± 0.301
2.243ProLys: 2.243 ± 0.284
2.617ProLeu: 2.617 ± 0.457
0.623ProMet: 0.623 ± 0.17
1.682ProAsn: 1.682 ± 0.473
1.246ProPro: 1.246 ± 0.352
1.869ProGln: 1.869 ± 0.409
1.246ProArg: 1.246 ± 0.283
2.181ProSer: 2.181 ± 0.372
2.056ProThr: 2.056 ± 0.377
1.932ProVal: 1.932 ± 0.334
0.685ProTrp: 0.685 ± 0.243
1.059ProTyr: 1.059 ± 0.321
0.0ProXaa: 0.0 ± 0.0
Gln
3.178GlnAla: 3.178 ± 0.416
0.312GlnCys: 0.312 ± 0.144
2.119GlnAsp: 2.119 ± 0.381
2.991GlnGlu: 2.991 ± 0.433
1.682GlnPhe: 1.682 ± 0.344
2.368GlnGly: 2.368 ± 0.379
0.187GlnHis: 0.187 ± 0.098
2.43GlnIle: 2.43 ± 0.616
2.991GlnLys: 2.991 ± 0.51
3.925GlnLeu: 3.925 ± 0.495
0.997GlnMet: 0.997 ± 0.232
1.433GlnAsn: 1.433 ± 0.309
0.81GlnPro: 0.81 ± 0.228
1.246GlnGln: 1.246 ± 0.317
2.056GlnArg: 2.056 ± 0.376
1.558GlnSer: 1.558 ± 0.364
2.305GlnThr: 2.305 ± 0.395
2.866GlnVal: 2.866 ± 0.477
0.125GlnTrp: 0.125 ± 0.064
1.558GlnTyr: 1.558 ± 0.306
0.0GlnXaa: 0.0 ± 0.0
Arg
3.489ArgAla: 3.489 ± 0.411
0.498ArgCys: 0.498 ± 0.211
2.43ArgAsp: 2.43 ± 0.362
4.299ArgGlu: 4.299 ± 0.524
1.807ArgPhe: 1.807 ± 0.406
2.866ArgGly: 2.866 ± 0.398
0.561ArgHis: 0.561 ± 0.174
3.24ArgIle: 3.24 ± 0.482
3.552ArgLys: 3.552 ± 0.551
3.24ArgLeu: 3.24 ± 0.43
1.308ArgMet: 1.308 ± 0.321
1.807ArgAsn: 1.807 ± 0.374
0.997ArgPro: 0.997 ± 0.217
1.62ArgGln: 1.62 ± 0.321
2.305ArgArg: 2.305 ± 0.364
2.181ArgSer: 2.181 ± 0.392
2.43ArgThr: 2.43 ± 0.409
2.617ArgVal: 2.617 ± 0.415
0.498ArgTrp: 0.498 ± 0.183
1.869ArgTyr: 1.869 ± 0.335
0.0ArgXaa: 0.0 ± 0.0
Ser
3.863SerAla: 3.863 ± 0.538
0.748SerCys: 0.748 ± 0.244
3.178SerAsp: 3.178 ± 0.602
4.486SerGlu: 4.486 ± 0.602
2.679SerPhe: 2.679 ± 0.413
4.985SerGly: 4.985 ± 0.51
1.059SerHis: 1.059 ± 0.216
3.552SerIle: 3.552 ± 0.453
5.732SerLys: 5.732 ± 0.515
5.732SerLeu: 5.732 ± 0.515
1.932SerMet: 1.932 ± 0.323
3.427SerAsn: 3.427 ± 0.434
1.994SerPro: 1.994 ± 0.4
2.492SerGln: 2.492 ± 0.396
2.056SerArg: 2.056 ± 0.392
4.299SerSer: 4.299 ± 0.9
3.427SerThr: 3.427 ± 0.512
3.988SerVal: 3.988 ± 0.5
0.81SerTrp: 0.81 ± 0.27
2.679SerTyr: 2.679 ± 0.436
0.0SerXaa: 0.0 ± 0.0
Thr
4.299ThrAla: 4.299 ± 0.647
0.498ThrCys: 0.498 ± 0.203
3.24ThrAsp: 3.24 ± 0.449
4.985ThrGlu: 4.985 ± 0.645
2.679ThrPhe: 2.679 ± 0.326
4.486ThrGly: 4.486 ± 0.495
0.935ThrHis: 0.935 ± 0.234
4.922ThrIle: 4.922 ± 0.49
2.305ThrLys: 2.305 ± 0.32
5.67ThrLeu: 5.67 ± 0.734
1.122ThrMet: 1.122 ± 0.22
2.929ThrAsn: 2.929 ± 0.502
3.302ThrPro: 3.302 ± 0.472
1.994ThrGln: 1.994 ± 0.374
2.119ThrArg: 2.119 ± 0.344
3.988ThrSer: 3.988 ± 0.632
3.739ThrThr: 3.739 ± 0.749
4.237ThrVal: 4.237 ± 0.507
0.374ThrTrp: 0.374 ± 0.155
2.742ThrTyr: 2.742 ± 0.447
0.0ThrXaa: 0.0 ± 0.0
Val
3.925ValAla: 3.925 ± 0.514
0.685ValCys: 0.685 ± 0.232
3.988ValAsp: 3.988 ± 0.541
4.424ValGlu: 4.424 ± 0.507
1.807ValPhe: 1.807 ± 0.365
3.614ValGly: 3.614 ± 0.61
0.498ValHis: 0.498 ± 0.157
4.985ValIle: 4.985 ± 0.529
5.421ValLys: 5.421 ± 0.596
3.552ValLeu: 3.552 ± 0.47
2.056ValMet: 2.056 ± 0.367
3.178ValAsn: 3.178 ± 0.467
1.994ValPro: 1.994 ± 0.294
2.056ValGln: 2.056 ± 0.393
2.617ValArg: 2.617 ± 0.345
4.486ValSer: 4.486 ± 0.539
4.237ValThr: 4.237 ± 0.608
3.988ValVal: 3.988 ± 0.607
0.561ValTrp: 0.561 ± 0.16
3.24ValTyr: 3.24 ± 0.45
0.0ValXaa: 0.0 ± 0.0
Trp
0.436TrpAla: 0.436 ± 0.168
0.312TrpCys: 0.312 ± 0.124
0.748TrpAsp: 0.748 ± 0.228
1.246TrpGlu: 1.246 ± 0.343
0.374TrpPhe: 0.374 ± 0.168
0.748TrpGly: 0.748 ± 0.219
0.062TrpHis: 0.062 ± 0.062
0.935TrpIle: 0.935 ± 0.263
0.81TrpLys: 0.81 ± 0.192
0.685TrpLeu: 0.685 ± 0.232
0.436TrpMet: 0.436 ± 0.17
1.122TrpAsn: 1.122 ± 0.296
0.0TrpPro: 0.0 ± 0.0
0.623TrpGln: 0.623 ± 0.235
0.872TrpArg: 0.872 ± 0.24
1.308TrpSer: 1.308 ± 0.328
0.81TrpThr: 0.81 ± 0.167
0.498TrpVal: 0.498 ± 0.18
0.187TrpTrp: 0.187 ± 0.095
0.685TrpTyr: 0.685 ± 0.277
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.929TyrAla: 2.929 ± 0.46
0.561TyrCys: 0.561 ± 0.232
2.804TyrAsp: 2.804 ± 0.417
2.679TyrGlu: 2.679 ± 0.351
1.869TyrPhe: 1.869 ± 0.369
3.552TyrGly: 3.552 ± 0.511
0.748TyrHis: 0.748 ± 0.181
2.804TyrIle: 2.804 ± 0.425
3.863TyrLys: 3.863 ± 0.564
3.053TyrLeu: 3.053 ± 0.446
1.059TyrMet: 1.059 ± 0.283
2.305TyrAsn: 2.305 ± 0.395
1.682TyrPro: 1.682 ± 0.333
1.433TyrGln: 1.433 ± 0.27
1.246TyrArg: 1.246 ± 0.27
2.555TyrSer: 2.555 ± 0.343
2.991TyrThr: 2.991 ± 0.499
2.056TyrVal: 2.056 ± 0.452
0.935TyrTrp: 0.935 ± 0.253
2.119TyrTyr: 2.119 ± 0.668
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (16050 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski