Kouleothrix aurantiaca

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexia; Chloroflexales; Roseiflexineae; Roseiflexaceae; Kouleothrix

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7836 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P9CM06|A0A0P9CM06_9CHLR Transglycosylase (Fragment) OS=Kouleothrix aurantiaca OX=186479 GN=SE17_43010 PE=4 SV=1
GG1 pKa = 7.47DD2 pKa = 4.32ALSAVLVNSPAHH14 pKa = 5.78GTLTLNSDD22 pKa = 3.48SSFTYY27 pKa = 10.02TPNANYY33 pKa = 10.53NGADD37 pKa = 3.41GFSYY41 pKa = 10.45KK42 pKa = 10.98ANDD45 pKa = 3.48GTADD49 pKa = 3.66SNVAAVSITVNAVNDD64 pKa = 3.77APVASGQSVSTAEE77 pKa = 4.02DD78 pKa = 3.34TAKK81 pKa = 10.97NITLAANDD89 pKa = 4.06VEE91 pKa = 5.0SSSLTYY97 pKa = 10.04TVVSSPAHH105 pKa = 5.29GTLSGTAPNLTYY117 pKa = 10.33TPNANYY123 pKa = 10.3SGPDD127 pKa = 3.05SFTFKK132 pKa = 11.15ANDD135 pKa = 3.56GTLDD139 pKa = 3.83SNVATVNITVNAVNDD154 pKa = 3.75APTIAVAALTAFTVV168 pKa = 3.4

Molecular weight: 16.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P9DJT9|A0A0P9DJT9_9CHLR Uncharacterized protein OS=Kouleothrix aurantiaca OX=186479 GN=SE17_07680 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84ASWARR7 pKa = 11.84TWPVLRR13 pKa = 11.84PTLGWCMIGIGVLGIILPIIPGLPFLFPGIALVGRR48 pKa = 11.84RR49 pKa = 11.84SVALRR54 pKa = 11.84WAAVQVKK61 pKa = 9.38RR62 pKa = 11.84QLRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84AGVRR71 pKa = 11.84LPLLGPVGRR80 pKa = 11.84WALGAQHH87 pKa = 6.24QISRR91 pKa = 11.84QRR93 pKa = 11.84RR94 pKa = 11.84RR95 pKa = 11.84MAWWHH100 pKa = 4.17MEE102 pKa = 3.22RR103 pKa = 11.84RR104 pKa = 11.84RR105 pKa = 11.84RR106 pKa = 11.84QTQQQ110 pKa = 2.41

Molecular weight: 12.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7836

0

7836

1774083

31

2057

226.4

24.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.776 ± 0.045

0.818 ± 0.008

5.286 ± 0.024

5.359 ± 0.03

3.437 ± 0.02

8.081 ± 0.031

2.157 ± 0.017

4.979 ± 0.024

2.319 ± 0.022

10.996 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.162 ± 0.013

2.793 ± 0.021

5.536 ± 0.026

4.173 ± 0.023

7.15 ± 0.035

5.116 ± 0.024

5.569 ± 0.029

7.068 ± 0.026

1.55 ± 0.014

2.674 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski