Monosporascus sp. MC13-8B

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Xylariomycetidae; Xylariales; Xylariales incertae sedis; Monosporascus; unclassified Monosporascus

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10637 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4Q4UQP1|A0A4Q4UQP1_9PEZI Uncharacterized protein OS=Monosporascus sp. MC13-8B OX=2211646 GN=DL766_007648 PE=4 SV=1
MM1 pKa = 7.86HH2 pKa = 7.14ITTILSLTILSALGAAEE19 pKa = 4.45SNVDD23 pKa = 3.59RR24 pKa = 11.84DD25 pKa = 4.46DD26 pKa = 4.05VPSACLASCQSTIDD40 pKa = 3.44LSQRR44 pKa = 11.84CDD46 pKa = 3.21RR47 pKa = 11.84QTDD50 pKa = 3.19DD51 pKa = 4.06DD52 pKa = 4.82NIYY55 pKa = 10.33RR56 pKa = 11.84GCVCGAQDD64 pKa = 4.37AQRR67 pKa = 11.84QMDD70 pKa = 3.35QCAACVRR77 pKa = 11.84VNGMSGPVDD86 pKa = 3.37NDD88 pKa = 3.02VAEE91 pKa = 4.39LMNDD95 pKa = 3.75CGWDD99 pKa = 3.5FNTASGSATAVTSMTSRR116 pKa = 11.84TTAGSTPTPVVSTSTGASTTATITQTPTGASGSGATEE153 pKa = 3.91SSTTNSDD160 pKa = 3.06NAAPAATAGLAGLLVAAGAAVALFF184 pKa = 3.95

Molecular weight:
18.39 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4Q4W046|A0A4Q4W046_9PEZI Uncharacterized protein OS=Monosporascus sp. MC13-8B OX=2211646 GN=DL766_000428 PE=4 SV=1
MM1 pKa = 7.68PLCGCKK7 pKa = 9.76LHH9 pKa = 6.47VYY11 pKa = 10.05RR12 pKa = 11.84FRR14 pKa = 11.84LCRR17 pKa = 11.84HH18 pKa = 5.57YY19 pKa = 11.29GNPIKK24 pKa = 10.55HH25 pKa = 5.74EE26 pKa = 4.2CWRR29 pKa = 11.84ADD31 pKa = 3.48LRR33 pKa = 11.84TKK35 pKa = 10.13SPCMGSLFPCGKK47 pKa = 9.0PVKK50 pKa = 9.84AKK52 pKa = 10.22HH53 pKa = 6.26SRR55 pKa = 11.84DD56 pKa = 3.77GLCDD60 pKa = 2.91SCAAYY65 pKa = 9.65FAGFGDD71 pKa = 3.96RR72 pKa = 11.84SNEE75 pKa = 3.62VLAEE79 pKa = 3.77FLEE82 pKa = 4.6YY83 pKa = 10.48KK84 pKa = 10.41EE85 pKa = 3.99SRR87 pKa = 11.84GWRR90 pKa = 11.84KK91 pKa = 9.74KK92 pKa = 10.09RR93 pKa = 11.84VNPRR97 pKa = 11.84QVAPEE102 pKa = 3.97KK103 pKa = 10.33FVRR106 pKa = 11.84PDD108 pKa = 3.12ILLHH112 pKa = 5.78NSSVRR117 pKa = 11.84RR118 pKa = 11.84GGAQPFRR125 pKa = 11.84PQSPMRR131 pKa = 11.84ATLALLPRR139 pKa = 11.84NSHH142 pKa = 6.79LPQPPPPVRR151 pKa = 11.84QTQSGEE157 pKa = 3.49RR158 pKa = 11.84RR159 pKa = 11.84AEE161 pKa = 3.65RR162 pKa = 11.84RR163 pKa = 11.84ARR165 pKa = 11.84TPFEE169 pKa = 3.76HH170 pKa = 7.29ALPGDD175 pKa = 4.06CAASQSSSLILGDD188 pKa = 3.79RR189 pKa = 11.84LLMPNQGVYY198 pKa = 9.36TLPRR202 pKa = 11.84GHH204 pKa = 5.95STEE207 pKa = 4.09PCPLARR213 pKa = 11.84RR214 pKa = 11.84ITEE217 pKa = 3.61EE218 pKa = 4.32AEE220 pKa = 3.88RR221 pKa = 11.84LFPNPEE227 pKa = 3.56SAMYY231 pKa = 9.92PGVPKK236 pKa = 10.78LSAAPKK242 pKa = 8.16KK243 pKa = 9.88HH244 pKa = 5.78RR245 pKa = 11.84RR246 pKa = 11.84NHH248 pKa = 5.44PRR250 pKa = 11.84LPRR253 pKa = 11.84GNDD256 pKa = 2.78NKK258 pKa = 10.81APEE261 pKa = 4.5LTVVPAVIVSGGKK274 pKa = 9.68AFKK277 pKa = 10.4HH278 pKa = 5.78RR279 pKa = 11.84ADD281 pKa = 3.51AATAATRR288 pKa = 11.84SPSEE292 pKa = 4.25PLPAPTPSRR301 pKa = 11.84FFVLASPDD309 pKa = 3.28AMSPTGCSAHH319 pKa = 5.73VRR321 pKa = 11.84SLSLDD326 pKa = 3.25QTVAMPPPQRR336 pKa = 11.84LEE338 pKa = 3.95GVLAASCPQHH348 pKa = 6.55GEE350 pKa = 4.1RR351 pKa = 11.84YY352 pKa = 10.26DD353 pKa = 3.93FGCGEE358 pKa = 4.17CRR360 pKa = 11.84GGLFRR365 pKa = 11.84EE366 pKa = 4.22RR367 pKa = 11.84GLPTEE372 pKa = 4.23EE373 pKa = 3.45HH374 pKa = 6.33RR375 pKa = 11.84EE376 pKa = 4.24RR377 pKa = 11.84YY378 pKa = 9.77RR379 pKa = 11.84SMTPVSVAAAEE390 pKa = 4.21TSNNRR395 pKa = 11.84LGHH398 pKa = 5.91CRR400 pKa = 11.84STPLVVSVTTPRR412 pKa = 11.84RR413 pKa = 11.84SSPVPRR419 pKa = 11.84KK420 pKa = 9.53ACARR424 pKa = 11.84AAPSRR429 pKa = 11.84GTCVSPVTRR438 pKa = 11.84RR439 pKa = 11.84RR440 pKa = 11.84PSRR443 pKa = 11.84NRR445 pKa = 11.84SRR447 pKa = 11.84AVGSEE452 pKa = 4.0DD453 pKa = 3.26

Molecular weight:
49.94 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10637

0

10637

5133785

66

8847

482.6

53.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.304 ± 0.024

1.157 ± 0.008

5.896 ± 0.018

6.363 ± 0.024

3.508 ± 0.016

7.385 ± 0.022

2.338 ± 0.01

4.446 ± 0.015

4.601 ± 0.022

8.644 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.092 ± 0.008

3.49 ± 0.012

6.335 ± 0.027

3.855 ± 0.016

6.696 ± 0.022

7.877 ± 0.023

5.739 ± 0.017

6.151 ± 0.019

1.432 ± 0.007

2.691 ± 0.011

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski