Amino acid dipepetide frequency for Monosporascus sp. MC13-8B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.072AlaAla: 11.072 ± 0.068	1.061AlaCys: 1.061 ± 0.014	4.894AlaAsp: 4.894 ± 0.032	5.869AlaGlu: 5.869 ± 0.046	3.153AlaPhe: 3.153 ± 0.029	6.724AlaGly: 6.724 ± 0.04	1.894AlaHis: 1.894 ± 0.021	4.047AlaIle: 4.047 ± 0.03	4.177AlaLys: 4.177 ± 0.039	7.997AlaLeu: 7.997 ± 0.05	2.042AlaMet: 2.042 ± 0.025	3.01AlaAsn: 3.01 ± 0.027	5.389AlaPro: 5.389 ± 0.046	3.463AlaGln: 3.463 ± 0.03	5.931AlaArg: 5.931 ± 0.038	7.453AlaSer: 7.453 ± 0.04	5.527AlaThr: 5.527 ± 0.036	5.899AlaVal: 5.899 ± 0.042	1.192AlaTrp: 1.192 ± 0.017	2.25AlaTyr: 2.25 ± 0.022	0.0AlaXaa: 0.0 ± 0.0
Cys	0.97CysAla: 0.97 ± 0.015	0.221CysCys: 0.221 ± 0.007	0.653CysAsp: 0.653 ± 0.013	0.597CysGlu: 0.597 ± 0.012	0.482CysPhe: 0.482 ± 0.009	0.959CysGly: 0.959 ± 0.015	0.308CysHis: 0.308 ± 0.008	0.614CysIle: 0.614 ± 0.012	0.454CysLys: 0.454 ± 0.01	1.127CysLeu: 1.127 ± 0.015	0.248CysMet: 0.248 ± 0.008	0.397CysAsn: 0.397 ± 0.009	0.642CysPro: 0.642 ± 0.014	0.389CysGln: 0.389 ± 0.009	0.777CysArg: 0.777 ± 0.014	0.81CysSer: 0.81 ± 0.015	0.611CysThr: 0.611 ± 0.012	0.772CysVal: 0.772 ± 0.012	0.195CysTrp: 0.195 ± 0.006	0.347CysTyr: 0.347 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	5.345AspAla: 5.345 ± 0.038	0.602AspCys: 0.602 ± 0.011	4.743AspAsp: 4.743 ± 0.039	4.711AspGlu: 4.711 ± 0.036	2.171AspPhe: 2.171 ± 0.024	4.638AspGly: 4.638 ± 0.037	1.23AspHis: 1.23 ± 0.017	2.946AspIle: 2.946 ± 0.031	2.312AspLys: 2.312 ± 0.021	4.975AspLeu: 4.975 ± 0.034	1.259AspMet: 1.259 ± 0.016	1.872AspAsn: 1.872 ± 0.019	3.638AspPro: 3.638 ± 0.029	1.812AspGln: 1.812 ± 0.018	3.424AspArg: 3.424 ± 0.037	4.029AspSer: 4.029 ± 0.032	2.822AspThr: 2.822 ± 0.026	3.879AspVal: 3.879 ± 0.03	0.9AspTrp: 0.9 ± 0.015	1.648AspTyr: 1.648 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	6.055GluAla: 6.055 ± 0.042	0.628GluCys: 0.628 ± 0.014	4.435GluAsp: 4.435 ± 0.033	5.627GluGlu: 5.627 ± 0.051	1.971GluPhe: 1.971 ± 0.02	4.199GluGly: 4.199 ± 0.03	1.404GluHis: 1.404 ± 0.016	2.879GluIle: 2.879 ± 0.025	3.569GluLys: 3.569 ± 0.035	5.404GluLeu: 5.404 ± 0.039	1.417GluMet: 1.417 ± 0.019	2.251GluAsn: 2.251 ± 0.021	3.11GluPro: 3.11 ± 0.032	2.484GluGln: 2.484 ± 0.025	4.324GluArg: 4.324 ± 0.034	4.167GluSer: 4.167 ± 0.034	3.478GluThr: 3.478 ± 0.028	3.69GluVal: 3.69 ± 0.03	0.898GluTrp: 0.898 ± 0.014	1.645GluTyr: 1.645 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	3.048PheAla: 3.048 ± 0.028	0.506PheCys: 0.506 ± 0.009	2.222PheAsp: 2.222 ± 0.021	2.172PheGlu: 2.172 ± 0.021	1.439PhePhe: 1.439 ± 0.02	2.837PheGly: 2.837 ± 0.032	0.816PheHis: 0.816 ± 0.011	1.544PheIle: 1.544 ± 0.019	1.419PheLys: 1.419 ± 0.018	3.182PheLeu: 3.182 ± 0.03	0.746PheMet: 0.746 ± 0.012	1.283PheAsn: 1.283 ± 0.019	1.873PhePro: 1.873 ± 0.022	1.265PheGln: 1.265 ± 0.016	2.091PheArg: 2.091 ± 0.021	2.661PheSer: 2.661 ± 0.024	1.98PheThr: 1.98 ± 0.024	2.353PheVal: 2.353 ± 0.023	0.598PheTrp: 0.598 ± 0.012	1.048PheTyr: 1.048 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	6.337GlyAla: 6.337 ± 0.048	0.888GlyCys: 0.888 ± 0.016	4.332GlyAsp: 4.332 ± 0.033	4.103GlyGlu: 4.103 ± 0.032	2.752GlyPhe: 2.752 ± 0.025	7.13GlyGly: 7.13 ± 0.071	1.795GlyHis: 1.795 ± 0.017	3.364GlyIle: 3.364 ± 0.026	3.404GlyLys: 3.404 ± 0.027	6.107GlyLeu: 6.107 ± 0.039	1.57GlyMet: 1.57 ± 0.02	2.656GlyAsn: 2.656 ± 0.027	3.847GlyPro: 3.847 ± 0.037	2.561GlyGln: 2.561 ± 0.024	4.938GlyArg: 4.938 ± 0.038	5.991GlySer: 5.991 ± 0.041	4.124GlyThr: 4.124 ± 0.031	4.617GlyVal: 4.617 ± 0.033	1.183GlyTrp: 1.183 ± 0.018	2.147GlyTyr: 2.147 ± 0.024	0.0GlyXaa: 0.0 ± 0.0
His	1.918HisAla: 1.918 ± 0.022	0.296HisCys: 0.296 ± 0.008	1.307HisAsp: 1.307 ± 0.017	1.34HisGlu: 1.34 ± 0.016	0.868HisPhe: 0.868 ± 0.012	1.793HisGly: 1.793 ± 0.021	0.914HisHis: 0.914 ± 0.021	1.104HisIle: 1.104 ± 0.015	0.863HisLys: 0.863 ± 0.014	2.083HisLeu: 2.083 ± 0.019	0.488HisMet: 0.488 ± 0.01	0.806HisAsn: 0.806 ± 0.013	1.702HisPro: 1.702 ± 0.019	0.978HisGln: 0.978 ± 0.017	1.614HisArg: 1.614 ± 0.02	1.671HisSer: 1.671 ± 0.02	1.153HisThr: 1.153 ± 0.014	1.458HisVal: 1.458 ± 0.016	0.333HisTrp: 0.333 ± 0.008	0.689HisTyr: 0.689 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	3.938IleAla: 3.938 ± 0.029	0.664IleCys: 0.664 ± 0.013	2.705IleAsp: 2.705 ± 0.024	2.706IleGlu: 2.706 ± 0.022	1.72IlePhe: 1.72 ± 0.021	2.933IleGly: 2.933 ± 0.024	1.042IleHis: 1.042 ± 0.013	2.141IleIle: 2.141 ± 0.022	1.967IleLys: 1.967 ± 0.023	4.033IleLeu: 4.033 ± 0.027	0.937IleMet: 0.937 ± 0.015	1.605IleAsn: 1.605 ± 0.018	2.793IlePro: 2.793 ± 0.024	1.661IleGln: 1.661 ± 0.017	2.823IleArg: 2.823 ± 0.023	3.331IleSer: 3.331 ± 0.026	2.603IleThr: 2.603 ± 0.023	2.918IleVal: 2.918 ± 0.028	0.671IleTrp: 0.671 ± 0.013	1.269IleTyr: 1.269 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	4.33LysAla: 4.33 ± 0.036	0.43LysCys: 0.43 ± 0.01	2.644LysAsp: 2.644 ± 0.029	3.196LysGlu: 3.196 ± 0.035	1.376LysPhe: 1.376 ± 0.019	3.018LysGly: 3.018 ± 0.026	1.05LysHis: 1.05 ± 0.013	2.012LysIle: 2.012 ± 0.02	3.156LysLys: 3.156 ± 0.041	3.896LysLeu: 3.896 ± 0.032	0.936LysMet: 0.936 ± 0.014	1.567LysAsn: 1.567 ± 0.02	2.765LysPro: 2.765 ± 0.031	1.752LysGln: 1.752 ± 0.019	3.405LysArg: 3.405 ± 0.034	3.226LysSer: 3.226 ± 0.033	2.646LysThr: 2.646 ± 0.023	2.713LysVal: 2.713 ± 0.025	0.624LysTrp: 0.624 ± 0.01	1.264LysTyr: 1.264 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	8.144LeuAla: 8.144 ± 0.053	1.125LeuCys: 1.125 ± 0.016	5.162LeuAsp: 5.162 ± 0.036	5.551LeuGlu: 5.551 ± 0.043	3.058LeuPhe: 3.058 ± 0.029	6.069LeuGly: 6.069 ± 0.042	2.04LeuHis: 2.04 ± 0.02	3.449LeuIle: 3.449 ± 0.027	3.955LeuLys: 3.955 ± 0.031	8.082LeuLeu: 8.082 ± 0.054	1.718LeuMet: 1.718 ± 0.02	2.899LeuAsn: 2.899 ± 0.023	5.432LeuPro: 5.432 ± 0.034	3.494LeuGln: 3.494 ± 0.03	6.237LeuArg: 6.237 ± 0.044	6.777LeuSer: 6.777 ± 0.04	4.499LeuThr: 4.499 ± 0.03	5.348LeuVal: 5.348 ± 0.035	1.185LeuTrp: 1.185 ± 0.017	2.213LeuTyr: 2.213 ± 0.023	0.0LeuXaa: 0.0 ± 0.0
Met	2.274MetAla: 2.274 ± 0.023	0.228MetCys: 0.228 ± 0.007	1.235MetAsp: 1.235 ± 0.016	1.256MetGlu: 1.256 ± 0.015	0.691MetPhe: 0.691 ± 0.011	1.439MetGly: 1.439 ± 0.018	0.462MetHis: 0.462 ± 0.011	0.883MetIle: 0.883 ± 0.014	0.939MetLys: 0.939 ± 0.014	1.779MetLeu: 1.779 ± 0.02	0.546MetMet: 0.546 ± 0.012	0.74MetAsn: 0.74 ± 0.013	1.286MetPro: 1.286 ± 0.015	0.805MetGln: 0.805 ± 0.014	1.374MetArg: 1.374 ± 0.017	1.708MetSer: 1.708 ± 0.02	1.265MetThr: 1.265 ± 0.014	1.264MetVal: 1.264 ± 0.016	0.254MetTrp: 0.254 ± 0.007	0.488MetTyr: 0.488 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.13AsnAla: 3.13 ± 0.027	0.408AsnCys: 0.408 ± 0.009	1.864AsnAsp: 1.864 ± 0.02	1.958AsnGlu: 1.958 ± 0.019	1.265AsnPhe: 1.265 ± 0.016	3.041AsnGly: 3.041 ± 0.026	0.808AsnHis: 0.808 ± 0.013	1.792AsnIle: 1.792 ± 0.02	1.453AsnLys: 1.453 ± 0.016	3.016AsnLeu: 3.016 ± 0.023	0.774AsnMet: 0.774 ± 0.012	1.481AsnAsn: 1.481 ± 0.023	2.467AsnPro: 2.467 ± 0.024	1.2AsnGln: 1.2 ± 0.015	2.033AsnArg: 2.033 ± 0.018	2.548AsnSer: 2.548 ± 0.025	1.971AsnThr: 1.971 ± 0.023	2.159AsnVal: 2.159 ± 0.022	0.523AsnTrp: 0.523 ± 0.009	1.008AsnTyr: 1.008 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	5.871ProAla: 5.871 ± 0.046	0.509ProCys: 0.509 ± 0.009	3.463ProAsp: 3.463 ± 0.029	4.152ProGlu: 4.152 ± 0.031	1.992ProPhe: 1.992 ± 0.017	4.609ProGly: 4.609 ± 0.04	1.353ProHis: 1.353 ± 0.018	2.32ProIle: 2.32 ± 0.02	2.651ProLys: 2.651 ± 0.029	4.808ProLeu: 4.808 ± 0.031	1.085ProMet: 1.085 ± 0.016	2.181ProAsn: 2.181 ± 0.025	5.902ProPro: 5.902 ± 0.062	2.543ProGln: 2.543 ± 0.031	3.959ProArg: 3.959 ± 0.034	5.975ProSer: 5.975 ± 0.043	4.029ProThr: 4.029 ± 0.036	3.635ProVal: 3.635 ± 0.031	0.762ProTrp: 0.762 ± 0.012	1.547ProTyr: 1.547 ± 0.022	0.001ProXaa: 0.001 ± 0.0
Gln	3.488GlnAla: 3.488 ± 0.031	0.4GlnCys: 0.4 ± 0.01	1.978GlnAsp: 1.978 ± 0.02	2.343GlnGlu: 2.343 ± 0.022	1.213GlnPhe: 1.213 ± 0.015	2.471GlnGly: 2.471 ± 0.023	1.074GlnHis: 1.074 ± 0.016	1.667GlnIle: 1.667 ± 0.018	1.848GlnLys: 1.848 ± 0.019	3.254GlnLeu: 3.254 ± 0.027	0.811GlnMet: 0.811 ± 0.014	1.368GlnAsn: 1.368 ± 0.018	2.67GlnPro: 2.67 ± 0.028	2.615GlnGln: 2.615 ± 0.052	2.728GlnArg: 2.728 ± 0.029	2.823GlnSer: 2.823 ± 0.029	2.095GlnThr: 2.095 ± 0.021	2.073GlnVal: 2.073 ± 0.02	0.538GlnTrp: 0.538 ± 0.011	1.093GlnTyr: 1.093 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	5.589ArgAla: 5.589 ± 0.036	0.746ArgCys: 0.746 ± 0.013	3.983ArgAsp: 3.983 ± 0.038	4.333ArgGlu: 4.333 ± 0.04	2.211ArgPhe: 2.211 ± 0.022	4.531ArgGly: 4.531 ± 0.035	1.744ArgHis: 1.744 ± 0.023	2.918ArgIle: 2.918 ± 0.024	3.445ArgLys: 3.445 ± 0.031	5.88ArgLeu: 5.88 ± 0.042	1.385ArgMet: 1.385 ± 0.016	2.381ArgAsn: 2.381 ± 0.023	4.041ArgPro: 4.041 ± 0.032	2.812ArgGln: 2.812 ± 0.027	6.123ArgArg: 6.123 ± 0.047	4.936ArgSer: 4.936 ± 0.041	3.457ArgThr: 3.457 ± 0.027	3.699ArgVal: 3.699 ± 0.027	1.014ArgTrp: 1.014 ± 0.014	1.727ArgTyr: 1.727 ± 0.021	0.0ArgXaa: 0.0 ± 0.0
Ser	6.885SerAla: 6.885 ± 0.042	0.796SerCys: 0.796 ± 0.015	4.078SerAsp: 4.078 ± 0.032	4.123SerGlu: 4.123 ± 0.034	2.724SerPhe: 2.724 ± 0.024	5.868SerGly: 5.868 ± 0.036	1.813SerHis: 1.813 ± 0.02	3.42SerIle: 3.42 ± 0.025	3.399SerLys: 3.399 ± 0.031	6.611SerLeu: 6.611 ± 0.04	1.59SerMet: 1.59 ± 0.017	2.747SerAsn: 2.747 ± 0.025	5.566SerPro: 5.566 ± 0.047	3.053SerGln: 3.053 ± 0.029	5.267SerArg: 5.267 ± 0.043	8.357SerSer: 8.357 ± 0.072	5.07SerThr: 5.07 ± 0.047	4.364SerVal: 4.364 ± 0.029	1.075SerTrp: 1.075 ± 0.013	1.964SerTyr: 1.964 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	5.469ThrAla: 5.469 ± 0.035	0.701ThrCys: 0.701 ± 0.012	2.73ThrAsp: 2.73 ± 0.023	3.108ThrGlu: 3.108 ± 0.03	2.01ThrPhe: 2.01 ± 0.02	4.271ThrGly: 4.271 ± 0.035	1.182ThrHis: 1.182 ± 0.017	2.726ThrIle: 2.726 ± 0.024	2.447ThrLys: 2.447 ± 0.027	4.78ThrLeu: 4.78 ± 0.034	1.146ThrMet: 1.146 ± 0.017	1.911ThrAsn: 1.911 ± 0.022	4.38ThrPro: 4.38 ± 0.043	1.925ThrGln: 1.925 ± 0.022	3.334ThrArg: 3.334 ± 0.027	5.019ThrSer: 5.019 ± 0.039	4.243ThrThr: 4.243 ± 0.054	3.648ThrVal: 3.648 ± 0.029	0.82ThrTrp: 0.82 ± 0.013	1.536ThrTyr: 1.536 ± 0.019	0.0ThrXaa: 0.0 ± 0.0
Val	5.729ValAla: 5.729 ± 0.035	0.786ValCys: 0.786 ± 0.012	3.874ValAsp: 3.874 ± 0.028	4.013ValGlu: 4.013 ± 0.032	2.346ValPhe: 2.346 ± 0.024	4.182ValGly: 4.182 ± 0.034	1.362ValHis: 1.362 ± 0.02	2.739ValIle: 2.739 ± 0.025	2.777ValLys: 2.777 ± 0.024	5.573ValLeu: 5.573 ± 0.039	1.275ValMet: 1.275 ± 0.016	2.128ValAsn: 2.128 ± 0.023	3.75ValPro: 3.75 ± 0.028	2.212ValGln: 2.212 ± 0.019	3.817ValArg: 3.817 ± 0.031	4.425ValSer: 4.425 ± 0.031	3.495ValThr: 3.495 ± 0.03	4.48ValVal: 4.48 ± 0.038	0.849ValTrp: 0.849 ± 0.014	1.7ValTyr: 1.7 ± 0.021	0.0ValXaa: 0.0 ± 0.0
Trp	1.219TrpAla: 1.219 ± 0.015	0.188TrpCys: 0.188 ± 0.007	0.946TrpAsp: 0.946 ± 0.015	0.874TrpGlu: 0.874 ± 0.014	0.527TrpPhe: 0.527 ± 0.011	0.972TrpGly: 0.972 ± 0.018	0.338TrpHis: 0.338 ± 0.008	0.685TrpIle: 0.685 ± 0.011	0.744TrpLys: 0.744 ± 0.011	1.333TrpLeu: 1.333 ± 0.019	0.346TrpMet: 0.346 ± 0.008	0.589TrpAsn: 0.589 ± 0.012	0.605TrpPro: 0.605 ± 0.012	0.525TrpGln: 0.525 ± 0.011	1.043TrpArg: 1.043 ± 0.015	0.961TrpSer: 0.961 ± 0.015	0.855TrpThr: 0.855 ± 0.012	0.886TrpVal: 0.886 ± 0.014	0.271TrpTrp: 0.271 ± 0.007	0.409TrpTyr: 0.409 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.236TyrAla: 2.236 ± 0.02	0.389TyrCys: 0.389 ± 0.008	1.71TyrAsp: 1.71 ± 0.023	1.601TyrGlu: 1.601 ± 0.018	1.112TyrPhe: 1.112 ± 0.018	2.161TyrGly: 2.161 ± 0.023	0.748TyrHis: 0.748 ± 0.013	1.209TyrIle: 1.209 ± 0.017	1.027TyrLys: 1.027 ± 0.015	2.515TyrLeu: 2.515 ± 0.027	0.599TyrMet: 0.599 ± 0.01	1.027TyrAsn: 1.027 ± 0.016	1.489TyrPro: 1.489 ± 0.019	1.0TyrGln: 1.0 ± 0.016	1.712TyrArg: 1.712 ± 0.017	1.904TyrSer: 1.904 ± 0.024	1.465TyrThr: 1.465 ± 0.015	1.658TyrVal: 1.658 ± 0.02	0.428TyrTrp: 0.428 ± 0.01	0.921TyrTyr: 0.921 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.01XaaXaa: 0.01 ± 0.007

Statistics based on 10637 proteins (5133785 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---