Operophtera brumata (winter moth)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Amphiesmenoptera; Lepidoptera; Glossata; Neolepidoptera; Heteroneura; Ditrysia;

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16814 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L7LJQ0|A0A0L7LJQ0_9NEOP Putative alcohol dehydrogenase (Fragment) OS=Operophtera brumata OX=104452 GN=OBRU01_07362 PE=3 SV=1
MM1 pKa = 7.46IRR3 pKa = 11.84AQTQSSAVLSTVHH16 pKa = 6.48TDD18 pKa = 2.99SVYY21 pKa = 10.73PEE23 pKa = 4.44PYY25 pKa = 10.65VNNTWVQIEE34 pKa = 4.29ASVLMVTVRR43 pKa = 11.84QLEE46 pKa = 4.35SKK48 pKa = 9.33YY49 pKa = 9.54QQPQEE54 pKa = 4.46CPGIDD59 pKa = 3.29NSGSGNPRR67 pKa = 11.84EE68 pKa = 4.17TSTTDD73 pKa = 2.83EE74 pKa = 4.83RR75 pKa = 11.84LTEE78 pKa = 4.88DD79 pKa = 5.76DD80 pKa = 5.69DD81 pKa = 7.38DD82 pKa = 6.1EE83 pKa = 8.08DD84 pKa = 6.84DD85 pKa = 6.58DD86 pKa = 7.49DD87 pKa = 7.58DD88 pKa = 7.66DD89 pKa = 7.66DD90 pKa = 7.69DD91 pKa = 7.57DD92 pKa = 7.29DD93 pKa = 7.38DD94 pKa = 6.39EE95 pKa = 8.1DD96 pKa = 6.84DD97 pKa = 6.52DD98 pKa = 7.49DD99 pKa = 7.58DD100 pKa = 7.62DD101 pKa = 7.68DD102 pKa = 7.67DD103 pKa = 7.3DD104 pKa = 7.52DD105 pKa = 6.36DD106 pKa = 5.54VADD109 pKa = 6.12DD110 pKa = 6.2DD111 pKa = 6.65DD112 pKa = 7.74DD113 pKa = 7.66DD114 pKa = 7.65DD115 pKa = 7.66DD116 pKa = 7.65DD117 pKa = 7.65DD118 pKa = 7.61DD119 pKa = 7.63DD120 pKa = 7.45DD121 pKa = 7.47DD122 pKa = 6.6DD123 pKa = 6.67DD124 pKa = 4.21STVTINSMKK133 pKa = 10.48LNRR136 pKa = 11.84NNAIYY141 pKa = 9.38MGKK144 pKa = 9.52LLLMDD149 pKa = 4.18TPTTVFLIWVLYY161 pKa = 9.97IVFVLKK167 pKa = 10.88LGPQLMKK174 pKa = 10.77NRR176 pKa = 11.84PPYY179 pKa = 10.21NLNNILTIYY188 pKa = 10.43NALQVVSSAYY198 pKa = 9.21VVYY201 pKa = 10.07MGAPILWNYY210 pKa = 8.08GLKK213 pKa = 10.21PKK215 pKa = 10.37CLNNTDD221 pKa = 4.5DD222 pKa = 4.65KK223 pKa = 11.19IGYY226 pKa = 9.14KK227 pKa = 10.24FNQVTFLHH235 pKa = 6.63VYY237 pKa = 7.39HH238 pKa = 6.87HH239 pKa = 7.14SYY241 pKa = 11.13VVISSWFIIKK251 pKa = 10.44NDD253 pKa = 3.27TTYY256 pKa = 12.0AMLFPAVLNSFIHH269 pKa = 5.63VVMYY273 pKa = 8.8TYY275 pKa = 10.99YY276 pKa = 10.99GLSAFPALAKK286 pKa = 9.81YY287 pKa = 9.32LWWKK291 pKa = 10.54KK292 pKa = 10.61YY293 pKa = 7.51ITAMQMKK300 pKa = 9.59II301 pKa = 3.33

Molecular weight: 34.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L7LQ86|A0A0L7LQ86_9NEOP Zinc/iron transporter OS=Operophtera brumata OX=104452 GN=OBRU01_04041 PE=4 SV=1
MM1 pKa = 7.21VPHH4 pKa = 7.16LPKK7 pKa = 10.43QGPLRR12 pKa = 11.84LNMVPHH18 pKa = 6.81LPKK21 pKa = 10.7LGPLHH26 pKa = 6.9LNMVPHH32 pKa = 6.84LPKK35 pKa = 10.7LGPLHH40 pKa = 6.9LNMVPHH46 pKa = 6.8LPKK49 pKa = 10.81LGTLRR54 pKa = 11.84LNMVPHH60 pKa = 7.02LPKK63 pKa = 10.42QGPLRR68 pKa = 11.84LNMVPHH74 pKa = 6.81LPKK77 pKa = 10.71LGPLRR82 pKa = 11.84LNMVPHH88 pKa = 7.02LPKK91 pKa = 10.42QGPLRR96 pKa = 11.84LNMVFHH102 pKa = 6.88LPKK105 pKa = 10.56LGPFRR110 pKa = 11.84LNMVPHH116 pKa = 6.87LPKK119 pKa = 10.66

Molecular weight: 13.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16814

0

16814

6022392

12

16700

358.2

40.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.03 ± 0.027

2.116 ± 0.034

5.506 ± 0.016

6.469 ± 0.026

3.574 ± 0.015

5.525 ± 0.024

2.624 ± 0.013

5.349 ± 0.017

6.313 ± 0.027

9.111 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.305 ± 0.011

4.72 ± 0.019

5.317 ± 0.031

3.872 ± 0.015

5.799 ± 0.025

7.727 ± 0.025

5.893 ± 0.023

6.38 ± 0.018

1.122 ± 0.008

3.243 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski