Amino acid dipepetide frequency for Faecalibacterium phage FP_Epona

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.698AlaAla: 14.698 ± 2.743
0.857AlaCys: 0.857 ± 0.239
6.327AlaAsp: 6.327 ± 0.585
7.25AlaGlu: 7.25 ± 0.59
3.164AlaPhe: 3.164 ± 0.555
6.789AlaGly: 6.789 ± 0.717
0.791AlaHis: 0.791 ± 0.193
5.932AlaIle: 5.932 ± 1.329
6.262AlaLys: 6.262 ± 0.572
6.987AlaLeu: 6.987 ± 1.094
3.032AlaMet: 3.032 ± 0.531
3.361AlaAsn: 3.361 ± 0.467
3.098AlaPro: 3.098 ± 0.457
3.823AlaGln: 3.823 ± 0.563
4.021AlaArg: 4.021 ± 0.572
4.548AlaSer: 4.548 ± 0.477
6.13AlaThr: 6.13 ± 0.576
6.855AlaVal: 6.855 ± 1.019
1.055AlaTrp: 1.055 ± 0.303
3.164AlaTyr: 3.164 ± 0.367
0.0AlaXaa: 0.0 ± 0.0
Cys
0.857CysAla: 0.857 ± 0.266
0.198CysCys: 0.198 ± 0.097
0.857CysAsp: 0.857 ± 0.241
0.791CysGlu: 0.791 ± 0.166
0.593CysPhe: 0.593 ± 0.166
1.516CysGly: 1.516 ± 0.427
0.132CysHis: 0.132 ± 0.099
0.264CysIle: 0.264 ± 0.112
1.318CysLys: 1.318 ± 0.294
0.923CysLeu: 0.923 ± 0.205
0.132CysMet: 0.132 ± 0.097
0.527CysAsn: 0.527 ± 0.193
0.857CysPro: 0.857 ± 0.244
0.461CysGln: 0.461 ± 0.141
1.12CysArg: 1.12 ± 0.252
0.857CysSer: 0.857 ± 0.207
0.989CysThr: 0.989 ± 0.282
0.725CysVal: 0.725 ± 0.213
0.264CysTrp: 0.264 ± 0.126
0.593CysTyr: 0.593 ± 0.197
0.0CysXaa: 0.0 ± 0.0
Asp
4.614AspAla: 4.614 ± 0.49
1.055AspCys: 1.055 ± 0.241
3.823AspAsp: 3.823 ± 0.51
3.361AspGlu: 3.361 ± 0.514
2.702AspPhe: 2.702 ± 0.395
5.734AspGly: 5.734 ± 0.467
0.923AspHis: 0.923 ± 0.287
3.164AspIle: 3.164 ± 0.395
3.361AspLys: 3.361 ± 0.538
6.064AspLeu: 6.064 ± 0.773
2.175AspMet: 2.175 ± 0.366
1.977AspAsn: 1.977 ± 0.376
3.23AspPro: 3.23 ± 0.598
2.109AspGln: 2.109 ± 0.339
3.23AspArg: 3.23 ± 0.373
3.098AspSer: 3.098 ± 0.415
3.691AspThr: 3.691 ± 0.427
3.427AspVal: 3.427 ± 0.408
0.791AspTrp: 0.791 ± 0.242
3.361AspTyr: 3.361 ± 0.526
0.0AspXaa: 0.0 ± 0.0
Glu
6.789GluAla: 6.789 ± 0.808
0.659GluCys: 0.659 ± 0.203
4.284GluAsp: 4.284 ± 0.533
3.955GluGlu: 3.955 ± 0.583
2.505GluPhe: 2.505 ± 0.336
3.23GluGly: 3.23 ± 0.391
1.318GluHis: 1.318 ± 0.261
3.757GluIle: 3.757 ± 0.603
4.152GluLys: 4.152 ± 0.57
4.943GluLeu: 4.943 ± 0.53
2.043GluMet: 2.043 ± 0.456
2.439GluAsn: 2.439 ± 0.377
2.966GluPro: 2.966 ± 0.457
2.768GluGln: 2.768 ± 0.346
3.361GluArg: 3.361 ± 0.631
4.482GluSer: 4.482 ± 0.619
3.296GluThr: 3.296 ± 0.398
5.866GluVal: 5.866 ± 0.814
1.516GluTrp: 1.516 ± 0.323
2.175GluTyr: 2.175 ± 0.292
0.0GluXaa: 0.0 ± 0.0
Phe
2.505PheAla: 2.505 ± 0.386
0.395PheCys: 0.395 ± 0.161
2.109PheAsp: 2.109 ± 0.347
1.78PheGlu: 1.78 ± 0.334
1.648PhePhe: 1.648 ± 0.316
2.505PheGly: 2.505 ± 0.416
0.461PheHis: 0.461 ± 0.183
2.307PheIle: 2.307 ± 0.39
1.78PheLys: 1.78 ± 0.408
2.702PheLeu: 2.702 ± 0.421
0.857PheMet: 0.857 ± 0.21
1.648PheAsn: 1.648 ± 0.293
0.923PhePro: 0.923 ± 0.237
1.648PheGln: 1.648 ± 0.344
2.571PheArg: 2.571 ± 0.486
2.636PheSer: 2.636 ± 0.335
2.505PheThr: 2.505 ± 0.417
2.307PheVal: 2.307 ± 0.35
0.659PheTrp: 0.659 ± 0.216
1.582PheTyr: 1.582 ± 0.372
0.0PheXaa: 0.0 ± 0.0
Gly
5.009GlyAla: 5.009 ± 0.659
0.791GlyCys: 0.791 ± 0.269
3.889GlyAsp: 3.889 ± 0.444
3.427GlyGlu: 3.427 ± 0.465
2.834GlyPhe: 2.834 ± 0.367
5.207GlyGly: 5.207 ± 0.634
1.12GlyHis: 1.12 ± 0.272
5.009GlyIle: 5.009 ± 0.656
5.668GlyLys: 5.668 ± 0.501
4.943GlyLeu: 4.943 ± 0.481
1.78GlyMet: 1.78 ± 0.36
3.757GlyAsn: 3.757 ± 0.489
2.175GlyPro: 2.175 ± 0.357
2.439GlyGln: 2.439 ± 0.38
2.966GlyArg: 2.966 ± 0.519
4.746GlySer: 4.746 ± 0.565
5.207GlyThr: 5.207 ± 0.553
4.218GlyVal: 4.218 ± 0.486
1.384GlyTrp: 1.384 ± 0.333
2.439GlyTyr: 2.439 ± 0.356
0.0GlyXaa: 0.0 ± 0.0
His
1.252HisAla: 1.252 ± 0.261
0.527HisCys: 0.527 ± 0.191
1.384HisAsp: 1.384 ± 0.308
1.055HisGlu: 1.055 ± 0.267
0.264HisPhe: 0.264 ± 0.118
0.923HisGly: 0.923 ± 0.223
0.395HisHis: 0.395 ± 0.15
0.923HisIle: 0.923 ± 0.267
1.252HisLys: 1.252 ± 0.284
0.857HisLeu: 0.857 ± 0.267
0.593HisMet: 0.593 ± 0.193
0.593HisAsn: 0.593 ± 0.208
0.659HisPro: 0.659 ± 0.198
0.725HisGln: 0.725 ± 0.232
1.055HisArg: 1.055 ± 0.285
0.659HisSer: 0.659 ± 0.19
0.857HisThr: 0.857 ± 0.248
0.791HisVal: 0.791 ± 0.214
0.132HisTrp: 0.132 ± 0.092
0.659HisTyr: 0.659 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
5.8IleAla: 5.8 ± 0.729
0.791IleCys: 0.791 ± 0.222
3.296IleAsp: 3.296 ± 0.432
4.614IleGlu: 4.614 ± 0.57
2.043IlePhe: 2.043 ± 0.364
4.218IleGly: 4.218 ± 0.503
0.989IleHis: 0.989 ± 0.221
3.296IleIle: 3.296 ± 0.49
3.625IleLys: 3.625 ± 0.424
4.614IleLeu: 4.614 ± 0.569
1.318IleMet: 1.318 ± 0.292
2.241IleAsn: 2.241 ± 0.495
2.636IlePro: 2.636 ± 0.388
2.571IleGln: 2.571 ± 0.398
3.823IleArg: 3.823 ± 0.673
3.493IleSer: 3.493 ± 0.681
4.548IleThr: 4.548 ± 0.433
3.032IleVal: 3.032 ± 0.355
0.659IleTrp: 0.659 ± 0.218
2.373IleTyr: 2.373 ± 0.347
0.0IleXaa: 0.0 ± 0.0
Lys
7.514LysAla: 7.514 ± 0.638
0.923LysCys: 0.923 ± 0.248
3.955LysAsp: 3.955 ± 0.475
4.943LysGlu: 4.943 ± 0.595
2.439LysPhe: 2.439 ± 0.344
3.361LysGly: 3.361 ± 0.428
0.725LysHis: 0.725 ± 0.192
4.021LysIle: 4.021 ± 0.527
5.734LysLys: 5.734 ± 0.646
5.668LysLeu: 5.668 ± 0.632
1.977LysMet: 1.977 ± 0.393
2.505LysAsn: 2.505 ± 0.393
2.439LysPro: 2.439 ± 0.415
2.702LysGln: 2.702 ± 0.433
3.427LysArg: 3.427 ± 0.556
3.164LysSer: 3.164 ± 0.452
3.955LysThr: 3.955 ± 0.588
3.493LysVal: 3.493 ± 0.403
1.252LysTrp: 1.252 ± 0.243
2.636LysTyr: 2.636 ± 0.411
0.0LysXaa: 0.0 ± 0.0
Leu
7.448LeuAla: 7.448 ± 0.709
1.318LeuCys: 1.318 ± 0.296
6.064LeuAsp: 6.064 ± 0.765
4.943LeuGlu: 4.943 ± 0.615
1.714LeuPhe: 1.714 ± 0.329
4.482LeuGly: 4.482 ± 0.653
1.582LeuHis: 1.582 ± 0.334
3.757LeuIle: 3.757 ± 0.511
5.734LeuLys: 5.734 ± 0.663
5.207LeuLeu: 5.207 ± 0.573
2.373LeuMet: 2.373 ± 0.436
3.559LeuAsn: 3.559 ± 0.462
3.098LeuPro: 3.098 ± 0.378
3.625LeuGln: 3.625 ± 0.505
3.23LeuArg: 3.23 ± 0.369
4.746LeuSer: 4.746 ± 0.727
4.943LeuThr: 4.943 ± 0.53
3.625LeuVal: 3.625 ± 0.549
0.989LeuTrp: 0.989 ± 0.252
2.175LeuTyr: 2.175 ± 0.335
0.0LeuXaa: 0.0 ± 0.0
Met
3.691MetAla: 3.691 ± 0.435
0.395MetCys: 0.395 ± 0.164
2.439MetAsp: 2.439 ± 0.393
1.977MetGlu: 1.977 ± 0.339
0.395MetPhe: 0.395 ± 0.176
1.582MetGly: 1.582 ± 0.307
0.461MetHis: 0.461 ± 0.124
1.186MetIle: 1.186 ± 0.214
2.439MetLys: 2.439 ± 0.409
2.043MetLeu: 2.043 ± 0.412
0.725MetMet: 0.725 ± 0.2
1.714MetAsn: 1.714 ± 0.365
0.989MetPro: 0.989 ± 0.271
1.12MetGln: 1.12 ± 0.249
1.384MetArg: 1.384 ± 0.359
1.12MetSer: 1.12 ± 0.233
2.043MetThr: 2.043 ± 0.34
1.45MetVal: 1.45 ± 0.263
0.132MetTrp: 0.132 ± 0.104
0.659MetTyr: 0.659 ± 0.201
0.0MetXaa: 0.0 ± 0.0
Asn
4.548AsnAla: 4.548 ± 0.705
0.923AsnCys: 0.923 ± 0.26
2.571AsnAsp: 2.571 ± 0.51
2.768AsnGlu: 2.768 ± 0.432
1.45AsnPhe: 1.45 ± 0.302
3.889AsnGly: 3.889 ± 0.497
0.791AsnHis: 0.791 ± 0.364
2.439AsnIle: 2.439 ± 0.342
2.241AsnLys: 2.241 ± 0.381
2.636AsnLeu: 2.636 ± 0.466
0.989AsnMet: 0.989 ± 0.224
1.714AsnAsn: 1.714 ± 0.308
2.043AsnPro: 2.043 ± 0.497
1.318AsnGln: 1.318 ± 0.356
2.241AsnArg: 2.241 ± 0.407
2.505AsnSer: 2.505 ± 0.379
2.636AsnThr: 2.636 ± 0.461
2.241AsnVal: 2.241 ± 0.426
0.593AsnTrp: 0.593 ± 0.196
1.055AsnTyr: 1.055 ± 0.255
0.0AsnXaa: 0.0 ± 0.0
Pro
4.746ProAla: 4.746 ± 0.654
0.791ProCys: 0.791 ± 0.234
2.571ProAsp: 2.571 ± 0.471
4.548ProGlu: 4.548 ± 0.647
1.977ProPhe: 1.977 ± 0.326
2.768ProGly: 2.768 ± 0.49
0.593ProHis: 0.593 ± 0.179
2.043ProIle: 2.043 ± 0.344
2.636ProLys: 2.636 ± 0.46
1.846ProLeu: 1.846 ± 0.354
0.791ProMet: 0.791 ± 0.269
1.384ProAsn: 1.384 ± 0.408
1.252ProPro: 1.252 ± 0.262
0.923ProGln: 0.923 ± 0.197
1.252ProArg: 1.252 ± 0.246
2.109ProSer: 2.109 ± 0.317
2.702ProThr: 2.702 ± 0.581
2.439ProVal: 2.439 ± 0.44
0.593ProTrp: 0.593 ± 0.182
1.384ProTyr: 1.384 ± 0.361
0.0ProXaa: 0.0 ± 0.0
Gln
2.439GlnAla: 2.439 ± 0.374
0.659GlnCys: 0.659 ± 0.231
1.846GlnAsp: 1.846 ± 0.457
2.307GlnGlu: 2.307 ± 0.407
1.252GlnPhe: 1.252 ± 0.265
2.307GlnGly: 2.307 ± 0.333
0.395GlnHis: 0.395 ± 0.175
2.636GlnIle: 2.636 ± 0.331
2.9GlnLys: 2.9 ± 0.372
2.9GlnLeu: 2.9 ± 0.45
1.516GlnMet: 1.516 ± 0.276
2.702GlnAsn: 2.702 ± 0.368
1.582GlnPro: 1.582 ± 0.322
2.636GlnGln: 2.636 ± 0.398
2.373GlnArg: 2.373 ± 0.323
2.966GlnSer: 2.966 ± 0.445
2.702GlnThr: 2.702 ± 0.336
2.505GlnVal: 2.505 ± 0.414
0.593GlnTrp: 0.593 ± 0.186
1.252GlnTyr: 1.252 ± 0.314
0.0GlnXaa: 0.0 ± 0.0
Arg
3.889ArgAla: 3.889 ± 0.553
0.395ArgCys: 0.395 ± 0.159
2.373ArgAsp: 2.373 ± 0.374
3.032ArgGlu: 3.032 ± 0.58
2.043ArgPhe: 2.043 ± 0.324
2.439ArgGly: 2.439 ± 0.403
1.384ArgHis: 1.384 ± 0.339
3.955ArgIle: 3.955 ± 0.598
4.218ArgLys: 4.218 ± 0.671
3.625ArgLeu: 3.625 ± 0.434
1.318ArgMet: 1.318 ± 0.329
2.307ArgAsn: 2.307 ± 0.376
1.45ArgPro: 1.45 ± 0.285
2.571ArgGln: 2.571 ± 0.303
3.823ArgArg: 3.823 ± 0.722
2.702ArgSer: 2.702 ± 0.424
3.955ArgThr: 3.955 ± 0.535
2.702ArgVal: 2.702 ± 0.524
0.857ArgTrp: 0.857 ± 0.215
1.78ArgTyr: 1.78 ± 0.409
0.0ArgXaa: 0.0 ± 0.0
Ser
4.548SerAla: 4.548 ± 1.324
0.593SerCys: 0.593 ± 0.211
3.032SerAsp: 3.032 ± 0.363
3.691SerGlu: 3.691 ± 0.622
2.439SerPhe: 2.439 ± 0.376
5.075SerGly: 5.075 ± 0.565
1.055SerHis: 1.055 ± 0.259
3.625SerIle: 3.625 ± 0.627
2.768SerLys: 2.768 ± 0.501
5.075SerLeu: 5.075 ± 0.579
1.384SerMet: 1.384 ± 0.291
2.505SerAsn: 2.505 ± 0.422
1.977SerPro: 1.977 ± 0.401
2.109SerGln: 2.109 ± 0.369
2.175SerArg: 2.175 ± 0.353
4.086SerSer: 4.086 ± 0.825
4.614SerThr: 4.614 ± 0.493
4.68SerVal: 4.68 ± 0.489
0.791SerTrp: 0.791 ± 0.217
2.307SerTyr: 2.307 ± 0.428
0.0SerXaa: 0.0 ± 0.0
Thr
8.305ThrAla: 8.305 ± 1.497
1.318ThrCys: 1.318 ± 0.281
3.955ThrAsp: 3.955 ± 0.416
4.152ThrGlu: 4.152 ± 0.565
1.846ThrPhe: 1.846 ± 0.323
5.141ThrGly: 5.141 ± 0.513
0.659ThrHis: 0.659 ± 0.227
4.746ThrIle: 4.746 ± 0.716
3.493ThrLys: 3.493 ± 0.441
4.548ThrLeu: 4.548 ± 0.598
1.911ThrMet: 1.911 ± 0.293
2.241ThrAsn: 2.241 ± 0.429
3.361ThrPro: 3.361 ± 0.499
2.109ThrGln: 2.109 ± 0.427
2.9ThrArg: 2.9 ± 0.467
3.955ThrSer: 3.955 ± 0.657
4.152ThrThr: 4.152 ± 0.587
4.548ThrVal: 4.548 ± 0.484
0.395ThrTrp: 0.395 ± 0.154
3.032ThrTyr: 3.032 ± 0.454
0.0ThrXaa: 0.0 ± 0.0
Val
4.746ValAla: 4.746 ± 0.551
0.857ValCys: 0.857 ± 0.21
3.955ValAsp: 3.955 ± 0.468
4.614ValGlu: 4.614 ± 0.66
2.505ValPhe: 2.505 ± 0.491
4.021ValGly: 4.021 ± 0.578
0.395ValHis: 0.395 ± 0.148
3.823ValIle: 3.823 ± 0.442
4.218ValLys: 4.218 ± 0.497
5.075ValLeu: 5.075 ± 0.508
1.582ValMet: 1.582 ± 0.369
2.241ValAsn: 2.241 ± 0.304
2.702ValPro: 2.702 ± 0.313
2.439ValGln: 2.439 ± 0.381
3.032ValArg: 3.032 ± 0.509
3.757ValSer: 3.757 ± 0.511
4.021ValThr: 4.021 ± 0.588
3.625ValVal: 3.625 ± 0.492
0.923ValTrp: 0.923 ± 0.22
2.636ValTyr: 2.636 ± 0.444
0.0ValXaa: 0.0 ± 0.0
Trp
1.252TrpAla: 1.252 ± 0.289
0.132TrpCys: 0.132 ± 0.084
0.593TrpAsp: 0.593 ± 0.213
1.055TrpGlu: 1.055 ± 0.325
0.659TrpPhe: 0.659 ± 0.198
1.12TrpGly: 1.12 ± 0.215
0.461TrpHis: 0.461 ± 0.171
0.857TrpIle: 0.857 ± 0.246
0.923TrpLys: 0.923 ± 0.245
1.384TrpLeu: 1.384 ± 0.366
0.395TrpMet: 0.395 ± 0.188
0.264TrpAsn: 0.264 ± 0.123
0.461TrpPro: 0.461 ± 0.167
0.857TrpGln: 0.857 ± 0.284
0.725TrpArg: 0.725 ± 0.23
0.527TrpSer: 0.527 ± 0.147
1.12TrpThr: 1.12 ± 0.274
0.725TrpVal: 0.725 ± 0.196
0.264TrpTrp: 0.264 ± 0.153
0.593TrpTyr: 0.593 ± 0.222
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.493TyrAla: 3.493 ± 0.421
0.395TyrCys: 0.395 ± 0.145
2.571TyrAsp: 2.571 ± 0.357
2.241TyrGlu: 2.241 ± 0.458
1.055TyrPhe: 1.055 ± 0.288
2.702TyrGly: 2.702 ± 0.48
0.923TyrHis: 0.923 ± 0.26
2.241TyrIle: 2.241 ± 0.322
2.175TyrLys: 2.175 ± 0.341
2.571TyrLeu: 2.571 ± 0.416
0.989TyrMet: 0.989 ± 0.221
1.911TyrAsn: 1.911 ± 0.369
1.45TyrPro: 1.45 ± 0.336
1.516TyrGln: 1.516 ± 0.329
2.109TyrArg: 2.109 ± 0.365
2.373TyrSer: 2.373 ± 0.419
2.702TyrThr: 2.702 ± 0.497
1.911TyrVal: 1.911 ± 0.423
0.527TyrTrp: 0.527 ± 0.207
1.12TyrTyr: 1.12 ± 0.307
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (15173 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski