Nostoc azollae (strain 0708) (Anabaena azollae (strain 0708))

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Trichormus; Trichormus azollae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3646 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7E571|D7E571_NOSA0 Uncharacterized protein OS=Nostoc azollae (strain 0708) OX=551115 GN=Aazo_1721 PE=4 SV=1
MM1 pKa = 7.17YY2 pKa = 10.18NYY4 pKa = 10.47VMANAAKK11 pKa = 10.06RR12 pKa = 11.84NQSQGFQVCYY22 pKa = 9.66IVMLIFVYY30 pKa = 11.05LLMTITDD37 pKa = 3.94DD38 pKa = 4.25GTRR41 pKa = 11.84IDD43 pKa = 3.84NNGIMFNDD51 pKa = 3.81DD52 pKa = 4.22SITLEE57 pKa = 4.11SALTAIDD64 pKa = 4.51LNSDD68 pKa = 4.39FIFEE72 pKa = 5.51LDD74 pKa = 3.45LTTNASDD81 pKa = 3.6GSYY84 pKa = 10.13TIADD88 pKa = 4.13FRR90 pKa = 11.84QSIQFSTPMIAIGTNWDD107 pKa = 3.15TYY109 pKa = 10.66EE110 pKa = 4.18RR111 pKa = 11.84VRR113 pKa = 11.84TT114 pKa = 3.87

Molecular weight: 12.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7E139|D7E139_NOSA0 D-aminoacyl-tRNA deacylase OS=Nostoc azollae (strain 0708) OX=551115 GN=dtd PE=3 SV=1
MM1 pKa = 7.08QRR3 pKa = 11.84TLGGTCRR10 pKa = 11.84KK11 pKa = 9.61RR12 pKa = 11.84KK13 pKa = 7.61RR14 pKa = 11.84TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TPEE26 pKa = 3.7GRR28 pKa = 11.84NVISARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.16GRR39 pKa = 11.84HH40 pKa = 4.95RR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3646

0

3646

942988

28

3291

258.6

28.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.692 ± 0.051

1.047 ± 0.013

4.668 ± 0.026

6.235 ± 0.044

3.862 ± 0.029

6.687 ± 0.041

1.955 ± 0.022

7.215 ± 0.035

5.028 ± 0.039

11.116 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.041 ± 0.018

4.416 ± 0.032

4.655 ± 0.027

5.24 ± 0.034

4.882 ± 0.032

6.311 ± 0.034

5.787 ± 0.032

6.713 ± 0.034

1.379 ± 0.017

3.072 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski