Amino acid dipepetide frequency for Mitsuokella sp. AF33-22

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.3AlaAla: 12.3 ± 0.218
1.392AlaCys: 1.392 ± 0.053
5.617AlaAsp: 5.617 ± 0.106
6.553AlaGlu: 6.553 ± 0.109
3.774AlaPhe: 3.774 ± 0.088
8.358AlaGly: 8.358 ± 0.138
2.007AlaHis: 2.007 ± 0.055
5.97AlaIle: 5.97 ± 0.119
5.271AlaLys: 5.271 ± 0.092
9.037AlaLeu: 9.037 ± 0.134
3.162AlaMet: 3.162 ± 0.07
2.643AlaAsn: 2.643 ± 0.068
3.064AlaPro: 3.064 ± 0.076
3.087AlaGln: 3.087 ± 0.073
5.113AlaArg: 5.113 ± 0.094
4.851AlaSer: 4.851 ± 0.093
3.921AlaThr: 3.921 ± 0.077
7.461AlaVal: 7.461 ± 0.111
0.929AlaTrp: 0.929 ± 0.04
3.057AlaTyr: 3.057 ± 0.066
0.0AlaXaa: 0.0 ± 0.0
Cys
1.07CysAla: 1.07 ± 0.042
0.245CysCys: 0.245 ± 0.018
0.629CysAsp: 0.629 ± 0.037
0.745CysGlu: 0.745 ± 0.039
0.551CysPhe: 0.551 ± 0.029
1.319CysGly: 1.319 ± 0.054
0.382CysHis: 0.382 ± 0.029
0.808CysIle: 0.808 ± 0.036
0.507CysLys: 0.507 ± 0.027
1.268CysLeu: 1.268 ± 0.044
0.362CysMet: 0.362 ± 0.025
0.364CysAsn: 0.364 ± 0.022
0.655CysPro: 0.655 ± 0.037
0.441CysGln: 0.441 ± 0.026
0.959CysArg: 0.959 ± 0.05
0.653CysSer: 0.653 ± 0.038
0.569CysThr: 0.569 ± 0.029
0.772CysVal: 0.772 ± 0.032
0.143CysTrp: 0.143 ± 0.014
0.45CysTyr: 0.45 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
5.336AspAla: 5.336 ± 0.097
0.71AspCys: 0.71 ± 0.035
3.063AspAsp: 3.063 ± 0.091
4.136AspGlu: 4.136 ± 0.09
2.813AspPhe: 2.813 ± 0.064
4.411AspGly: 4.411 ± 0.112
1.162AspHis: 1.162 ± 0.039
4.129AspIle: 4.129 ± 0.085
3.144AspLys: 3.144 ± 0.086
4.89AspLeu: 4.89 ± 0.091
1.744AspMet: 1.744 ± 0.049
1.626AspAsn: 1.626 ± 0.057
2.044AspPro: 2.044 ± 0.051
1.249AspGln: 1.249 ± 0.041
2.737AspArg: 2.737 ± 0.073
2.522AspSer: 2.522 ± 0.07
3.087AspThr: 3.087 ± 0.075
4.27AspVal: 4.27 ± 0.079
0.668AspTrp: 0.668 ± 0.038
2.334AspTyr: 2.334 ± 0.068
0.0AspXaa: 0.0 ± 0.0
Glu
6.863GluAla: 6.863 ± 0.118
0.589GluCys: 0.589 ± 0.028
3.626GluAsp: 3.626 ± 0.084
5.585GluGlu: 5.585 ± 0.111
2.057GluPhe: 2.057 ± 0.047
4.239GluGly: 4.239 ± 0.083
1.655GluHis: 1.655 ± 0.044
4.214GluIle: 4.214 ± 0.088
4.614GluLys: 4.614 ± 0.088
6.486GluLeu: 6.486 ± 0.097
1.9GluMet: 1.9 ± 0.051
2.696GluAsn: 2.696 ± 0.074
2.111GluPro: 2.111 ± 0.05
2.88GluGln: 2.88 ± 0.073
3.899GluArg: 3.899 ± 0.086
2.7GluSer: 2.7 ± 0.061
3.387GluThr: 3.387 ± 0.079
4.305GluVal: 4.305 ± 0.079
0.519GluTrp: 0.519 ± 0.026
1.944GluTyr: 1.944 ± 0.054
0.0GluXaa: 0.0 ± 0.0
Phe
3.883PheAla: 3.883 ± 0.092
0.62PheCys: 0.62 ± 0.029
2.469PheAsp: 2.469 ± 0.057
1.907PheGlu: 1.907 ± 0.053
1.954PhePhe: 1.954 ± 0.069
3.283PheGly: 3.283 ± 0.076
1.116PheHis: 1.116 ± 0.038
2.409PheIle: 2.409 ± 0.069
1.423PheLys: 1.423 ± 0.049
4.379PheLeu: 4.379 ± 0.108
1.085PheMet: 1.085 ± 0.042
1.281PheAsn: 1.281 ± 0.044
1.463PhePro: 1.463 ± 0.046
1.202PheGln: 1.202 ± 0.042
1.978PheArg: 1.978 ± 0.055
2.469PheSer: 2.469 ± 0.064
2.233PheThr: 2.233 ± 0.052
2.881PheVal: 2.881 ± 0.077
0.454PheTrp: 0.454 ± 0.028
1.466PheTyr: 1.466 ± 0.054
0.0PheXaa: 0.0 ± 0.0
Gly
6.465GlyAla: 6.465 ± 0.119
1.263GlyCys: 1.263 ± 0.048
3.727GlyAsp: 3.727 ± 0.089
4.497GlyGlu: 4.497 ± 0.088
3.318GlyPhe: 3.318 ± 0.074
5.636GlyGly: 5.636 ± 0.131
1.882GlyHis: 1.882 ± 0.054
5.862GlyIle: 5.862 ± 0.118
4.637GlyLys: 4.637 ± 0.091
7.16GlyLeu: 7.16 ± 0.118
2.426GlyMet: 2.426 ± 0.059
2.579GlyAsn: 2.579 ± 0.082
1.872GlyPro: 1.872 ± 0.056
2.376GlyGln: 2.376 ± 0.056
4.707GlyArg: 4.707 ± 0.086
4.227GlySer: 4.227 ± 0.083
4.764GlyThr: 4.764 ± 0.115
5.642GlyVal: 5.642 ± 0.082
0.831GlyTrp: 0.831 ± 0.032
2.646GlyTyr: 2.646 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
2.002HisAla: 2.002 ± 0.06
0.359HisCys: 0.359 ± 0.022
1.384HisAsp: 1.384 ± 0.05
1.613HisGlu: 1.613 ± 0.052
1.092HisPhe: 1.092 ± 0.04
1.901HisGly: 1.901 ± 0.053
0.752HisHis: 0.752 ± 0.032
1.644HisIle: 1.644 ± 0.049
0.988HisLys: 0.988 ± 0.035
2.247HisLeu: 2.247 ± 0.057
0.676HisMet: 0.676 ± 0.03
0.719HisAsn: 0.719 ± 0.032
1.268HisPro: 1.268 ± 0.046
0.754HisGln: 0.754 ± 0.033
1.233HisArg: 1.233 ± 0.042
1.042HisSer: 1.042 ± 0.033
1.297HisThr: 1.297 ± 0.045
1.652HisVal: 1.652 ± 0.05
0.242HisTrp: 0.242 ± 0.016
0.848HisTyr: 0.848 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
6.26IleAla: 6.26 ± 0.111
1.002IleCys: 1.002 ± 0.037
3.755IleAsp: 3.755 ± 0.081
3.972IleGlu: 3.972 ± 0.08
2.584IlePhe: 2.584 ± 0.081
5.039IleGly: 5.039 ± 0.102
1.554IleHis: 1.554 ± 0.053
3.875IleIle: 3.875 ± 0.095
2.794IleLys: 2.794 ± 0.073
6.089IleLeu: 6.089 ± 0.117
1.678IleMet: 1.678 ± 0.054
2.005IleAsn: 2.005 ± 0.063
2.899IlePro: 2.899 ± 0.072
1.855IleGln: 1.855 ± 0.058
3.591IleArg: 3.591 ± 0.08
3.429IleSer: 3.429 ± 0.07
3.507IleThr: 3.507 ± 0.079
4.711IleVal: 4.711 ± 0.099
0.559IleTrp: 0.559 ± 0.032
1.97IleTyr: 1.97 ± 0.05
0.0IleXaa: 0.0 ± 0.0
Lys
5.312LysAla: 5.312 ± 0.106
0.457LysCys: 0.457 ± 0.03
3.439LysAsp: 3.439 ± 0.087
4.191LysGlu: 4.191 ± 0.091
1.558LysPhe: 1.558 ± 0.045
3.663LysGly: 3.663 ± 0.079
1.021LysHis: 1.021 ± 0.038
3.376LysIle: 3.376 ± 0.072
3.958LysLys: 3.958 ± 0.081
4.687LysLeu: 4.687 ± 0.086
1.69LysMet: 1.69 ± 0.052
2.206LysAsn: 2.206 ± 0.075
2.045LysPro: 2.045 ± 0.056
1.89LysGln: 1.89 ± 0.048
2.954LysArg: 2.954 ± 0.065
2.514LysSer: 2.514 ± 0.071
2.998LysThr: 2.998 ± 0.071
3.661LysVal: 3.661 ± 0.094
0.535LysTrp: 0.535 ± 0.029
1.729LysTyr: 1.729 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
10.358LeuAla: 10.358 ± 0.169
1.283LeuCys: 1.283 ± 0.048
5.191LeuAsp: 5.191 ± 0.082
5.377LeuGlu: 5.377 ± 0.093
3.693LeuPhe: 3.693 ± 0.084
7.178LeuGly: 7.178 ± 0.124
2.252LeuHis: 2.252 ± 0.055
5.112LeuIle: 5.112 ± 0.111
4.912LeuLys: 4.912 ± 0.094
9.845LeuLeu: 9.845 ± 0.182
2.772LeuMet: 2.772 ± 0.081
2.791LeuAsn: 2.791 ± 0.064
4.877LeuPro: 4.877 ± 0.104
3.576LeuGln: 3.576 ± 0.082
5.569LeuArg: 5.569 ± 0.091
5.963LeuSer: 5.963 ± 0.092
5.882LeuThr: 5.882 ± 0.089
6.563LeuVal: 6.563 ± 0.117
0.851LeuTrp: 0.851 ± 0.038
2.946LeuTyr: 2.946 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
3.043MetAla: 3.043 ± 0.06
0.25MetCys: 0.25 ± 0.018
1.722MetAsp: 1.722 ± 0.054
2.005MetGlu: 2.005 ± 0.052
0.836MetPhe: 0.836 ± 0.033
2.163MetGly: 2.163 ± 0.071
0.655MetHis: 0.655 ± 0.035
1.57MetIle: 1.57 ± 0.048
2.085MetLys: 2.085 ± 0.053
2.772MetLeu: 2.772 ± 0.073
1.047MetMet: 1.047 ± 0.044
1.195MetAsn: 1.195 ± 0.044
1.4MetPro: 1.4 ± 0.043
1.17MetGln: 1.17 ± 0.036
1.533MetArg: 1.533 ± 0.05
1.572MetSer: 1.572 ± 0.055
1.851MetThr: 1.851 ± 0.055
1.858MetVal: 1.858 ± 0.057
0.159MetTrp: 0.159 ± 0.017
0.726MetTyr: 0.726 ± 0.036
0.0MetXaa: 0.0 ± 0.0
Asn
2.854AsnAla: 2.854 ± 0.072
0.389AsnCys: 0.389 ± 0.025
1.702AsnAsp: 1.702 ± 0.055
1.853AsnGlu: 1.853 ± 0.056
1.33AsnPhe: 1.33 ± 0.043
2.754AsnGly: 2.754 ± 0.091
0.839AsnHis: 0.839 ± 0.033
2.478AsnIle: 2.478 ± 0.064
1.583AsnLys: 1.583 ± 0.057
3.209AsnLeu: 3.209 ± 0.065
0.995AsnMet: 0.995 ± 0.04
1.139AsnAsn: 1.139 ± 0.058
1.931AsnPro: 1.931 ± 0.046
1.012AsnGln: 1.012 ± 0.033
1.729AsnArg: 1.729 ± 0.047
1.448AsnSer: 1.448 ± 0.056
1.675AsnThr: 1.675 ± 0.067
2.35AsnVal: 2.35 ± 0.068
0.376AsnTrp: 0.376 ± 0.023
1.304AsnTyr: 1.304 ± 0.057
0.0AsnXaa: 0.0 ± 0.0
Pro
4.009ProAla: 4.009 ± 0.108
0.464ProCys: 0.464 ± 0.027
2.563ProAsp: 2.563 ± 0.068
3.605ProGlu: 3.605 ± 0.069
1.757ProPhe: 1.757 ± 0.051
3.049ProGly: 3.049 ± 0.069
0.994ProHis: 0.994 ± 0.04
2.092ProIle: 2.092 ± 0.056
1.795ProLys: 1.795 ± 0.055
3.837ProLeu: 3.837 ± 0.077
1.03ProMet: 1.03 ± 0.034
1.181ProAsn: 1.181 ± 0.042
1.107ProPro: 1.107 ± 0.042
1.683ProGln: 1.683 ± 0.049
1.667ProArg: 1.667 ± 0.05
1.92ProSer: 1.92 ± 0.047
1.776ProThr: 1.776 ± 0.056
3.458ProVal: 3.458 ± 0.073
0.402ProTrp: 0.402 ± 0.027
1.424ProTyr: 1.424 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
3.859GlnAla: 3.859 ± 0.091
0.292GlnCys: 0.292 ± 0.022
2.092GlnAsp: 2.092 ± 0.053
3.001GlnGlu: 3.001 ± 0.083
1.031GlnPhe: 1.031 ± 0.039
2.459GlnGly: 2.459 ± 0.057
0.816GlnHis: 0.816 ± 0.042
1.713GlnIle: 1.713 ± 0.049
2.315GlnLys: 2.315 ± 0.063
3.201GlnLeu: 3.201 ± 0.064
1.035GlnMet: 1.035 ± 0.036
1.315GlnAsn: 1.315 ± 0.048
1.47GlnPro: 1.47 ± 0.047
2.033GlnGln: 2.033 ± 0.074
1.885GlnArg: 1.885 ± 0.052
1.585GlnSer: 1.585 ± 0.049
1.701GlnThr: 1.701 ± 0.051
2.255GlnVal: 2.255 ± 0.052
0.272GlnTrp: 0.272 ± 0.021
1.07GlnTyr: 1.07 ± 0.04
0.0GlnXaa: 0.0 ± 0.0
Arg
4.565ArgAla: 4.565 ± 0.093
0.682ArgCys: 0.682 ± 0.033
3.049ArgAsp: 3.049 ± 0.072
4.323ArgGlu: 4.323 ± 0.093
2.196ArgPhe: 2.196 ± 0.061
3.416ArgGly: 3.416 ± 0.082
1.466ArgHis: 1.466 ± 0.048
3.734ArgIle: 3.734 ± 0.073
3.067ArgLys: 3.067 ± 0.072
5.609ArgLeu: 5.609 ± 0.119
1.665ArgMet: 1.665 ± 0.048
1.831ArgAsn: 1.831 ± 0.05
2.049ArgPro: 2.049 ± 0.054
2.772ArgGln: 2.772 ± 0.074
3.962ArgArg: 3.962 ± 0.103
2.458ArgSer: 2.458 ± 0.064
2.729ArgThr: 2.729 ± 0.064
3.488ArgVal: 3.488 ± 0.079
0.536ArgTrp: 0.536 ± 0.026
2.143ArgTyr: 2.143 ± 0.062
0.0ArgXaa: 0.0 ± 0.0
Ser
4.379SerAla: 4.379 ± 0.075
0.715SerCys: 0.715 ± 0.038
2.552SerAsp: 2.552 ± 0.065
2.701SerGlu: 2.701 ± 0.059
2.509SerPhe: 2.509 ± 0.056
4.491SerGly: 4.491 ± 0.101
1.263SerHis: 1.263 ± 0.038
3.383SerIle: 3.383 ± 0.063
2.205SerLys: 2.205 ± 0.054
5.439SerLeu: 5.439 ± 0.099
1.577SerMet: 1.577 ± 0.047
1.553SerAsn: 1.553 ± 0.056
1.955SerPro: 1.955 ± 0.053
1.665SerGln: 1.665 ± 0.054
3.043SerArg: 3.043 ± 0.062
3.039SerSer: 3.039 ± 0.096
2.502SerThr: 2.502 ± 0.082
3.749SerVal: 3.749 ± 0.073
0.604SerTrp: 0.604 ± 0.032
1.955SerTyr: 1.955 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
5.367ThrAla: 5.367 ± 0.095
0.614ThrCys: 0.614 ± 0.03
2.967ThrAsp: 2.967 ± 0.08
2.939ThrGlu: 2.939 ± 0.067
2.065ThrPhe: 2.065 ± 0.053
4.971ThrGly: 4.971 ± 0.088
1.128ThrHis: 1.128 ± 0.036
3.723ThrIle: 3.723 ± 0.088
2.797ThrLys: 2.797 ± 0.077
5.029ThrLeu: 5.029 ± 0.093
1.542ThrMet: 1.542 ± 0.047
1.798ThrAsn: 1.798 ± 0.066
2.603ThrPro: 2.603 ± 0.057
1.566ThrGln: 1.566 ± 0.043
2.42ThrArg: 2.42 ± 0.064
2.824ThrSer: 2.824 ± 0.081
2.876ThrThr: 2.876 ± 0.092
4.194ThrVal: 4.194 ± 0.099
0.51ThrTrp: 0.51 ± 0.025
1.749ThrTyr: 1.749 ± 0.068
0.0ThrXaa: 0.0 ± 0.0
Val
6.091ValAla: 6.091 ± 0.109
1.033ValCys: 1.033 ± 0.038
3.841ValAsp: 3.841 ± 0.081
4.381ValGlu: 4.381 ± 0.096
2.869ValPhe: 2.869 ± 0.075
4.69ValGly: 4.69 ± 0.101
1.531ValHis: 1.531 ± 0.048
4.612ValIle: 4.612 ± 0.083
3.796ValLys: 3.796 ± 0.073
7.418ValLeu: 7.418 ± 0.122
2.12ValMet: 2.12 ± 0.06
2.479ValAsn: 2.479 ± 0.071
3.269ValPro: 3.269 ± 0.063
2.188ValGln: 2.188 ± 0.056
4.16ValArg: 4.16 ± 0.084
4.042ValSer: 4.042 ± 0.079
4.52ValThr: 4.52 ± 0.095
5.167ValVal: 5.167 ± 0.086
0.675ValTrp: 0.675 ± 0.032
2.28ValTyr: 2.28 ± 0.053
0.0ValXaa: 0.0 ± 0.0
Trp
0.675TrpAla: 0.675 ± 0.033
0.116TrpCys: 0.116 ± 0.012
0.48TrpAsp: 0.48 ± 0.026
0.559TrpGlu: 0.559 ± 0.032
0.44TrpPhe: 0.44 ± 0.028
0.686TrpGly: 0.686 ± 0.033
0.319TrpHis: 0.319 ± 0.023
0.52TrpIle: 0.52 ± 0.033
0.522TrpLys: 0.522 ± 0.025
1.092TrpLeu: 1.092 ± 0.043
0.286TrpMet: 0.286 ± 0.019
0.432TrpAsn: 0.432 ± 0.029
0.364TrpPro: 0.364 ± 0.021
0.749TrpGln: 0.749 ± 0.037
0.577TrpArg: 0.577 ± 0.026
0.516TrpSer: 0.516 ± 0.03
0.448TrpThr: 0.448 ± 0.025
0.492TrpVal: 0.492 ± 0.029
0.12TrpTrp: 0.12 ± 0.012
0.335TrpTyr: 0.335 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.876TyrAla: 2.876 ± 0.065
0.419TyrCys: 0.419 ± 0.026
2.205TyrAsp: 2.205 ± 0.062
2.327TyrGlu: 2.327 ± 0.051
1.526TyrPhe: 1.526 ± 0.044
2.809TyrGly: 2.809 ± 0.076
0.922TyrHis: 0.922 ± 0.041
1.904TyrIle: 1.904 ± 0.049
1.475TyrLys: 1.475 ± 0.049
3.298TyrLeu: 3.298 ± 0.068
0.819TyrMet: 0.819 ± 0.032
1.117TyrAsn: 1.117 ± 0.04
1.37TyrPro: 1.37 ± 0.041
1.25TyrGln: 1.25 ± 0.05
2.024TyrArg: 2.024 ± 0.057
1.503TyrSer: 1.503 ± 0.049
1.958TyrThr: 1.958 ± 0.082
2.233TyrVal: 2.233 ± 0.054
0.342TyrTrp: 0.342 ± 0.025
1.261TyrTyr: 1.261 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2221 proteins (743751 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski