Amino acid dipepetide frequency for filamentous cyanobacterium CCP2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.367AlaAla: 8.367 ± 0.085	0.85AlaCys: 0.85 ± 0.023	4.652AlaAsp: 4.652 ± 0.051	6.176AlaGlu: 6.176 ± 0.072	3.351AlaPhe: 3.351 ± 0.05	5.855AlaGly: 5.855 ± 0.069	1.621AlaHis: 1.621 ± 0.032	6.938AlaIle: 6.938 ± 0.062	3.374AlaLys: 3.374 ± 0.05	9.495AlaLeu: 9.495 ± 0.083	1.892AlaMet: 1.892 ± 0.032	3.297AlaAsn: 3.297 ± 0.058	3.481AlaPro: 3.481 ± 0.05	4.671AlaGln: 4.671 ± 0.058	4.037AlaArg: 4.037 ± 0.05	5.165AlaSer: 5.165 ± 0.068	4.877AlaThr: 4.877 ± 0.061	6.043AlaVal: 6.043 ± 0.059	1.171AlaTrp: 1.171 ± 0.028	2.372AlaTyr: 2.372 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	0.664CysAla: 0.664 ± 0.021	0.155CysCys: 0.155 ± 0.009	0.539CysAsp: 0.539 ± 0.019	0.492CysGlu: 0.492 ± 0.018	0.397CysPhe: 0.397 ± 0.014	0.748CysGly: 0.748 ± 0.021	0.299CysHis: 0.299 ± 0.013	0.552CysIle: 0.552 ± 0.017	0.276CysLys: 0.276 ± 0.012	1.139CysLeu: 1.139 ± 0.029	0.167CysMet: 0.167 ± 0.009	0.323CysAsn: 0.323 ± 0.015	0.569CysPro: 0.569 ± 0.017	0.591CysGln: 0.591 ± 0.018	0.574CysArg: 0.574 ± 0.019	0.594CysSer: 0.594 ± 0.017	0.51CysThr: 0.51 ± 0.019	0.582CysVal: 0.582 ± 0.018	0.163CysTrp: 0.163 ± 0.01	0.316CysTyr: 0.316 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	4.316AspAla: 4.316 ± 0.051	0.57AspCys: 0.57 ± 0.017	2.595AspAsp: 2.595 ± 0.047	3.294AspGlu: 3.294 ± 0.048	2.214AspPhe: 2.214 ± 0.037	3.548AspGly: 3.548 ± 0.061	0.96AspHis: 0.96 ± 0.026	2.722AspIle: 2.722 ± 0.039	1.401AspLys: 1.401 ± 0.032	6.161AspLeu: 6.161 ± 0.066	0.804AspMet: 0.804 ± 0.021	1.46AspAsn: 1.46 ± 0.03	2.89AspPro: 2.89 ± 0.043	2.307AspGln: 2.307 ± 0.037	5.118AspArg: 5.118 ± 0.051	2.761AspSer: 2.761 ± 0.036	2.414AspThr: 2.414 ± 0.043	3.345AspVal: 3.345 ± 0.045	1.002AspTrp: 1.002 ± 0.024	1.779AspTyr: 1.779 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	6.373GluAla: 6.373 ± 0.068	0.475GluCys: 0.475 ± 0.016	2.889GluAsp: 2.889 ± 0.039	4.069GluGlu: 4.069 ± 0.068	2.448GluPhe: 2.448 ± 0.039	3.668GluGly: 3.668 ± 0.047	1.085GluHis: 1.085 ± 0.028	3.995GluIle: 3.995 ± 0.045	2.364GluLys: 2.364 ± 0.044	6.828GluLeu: 6.828 ± 0.069	1.521GluMet: 1.521 ± 0.029	2.188GluAsn: 2.188 ± 0.036	3.054GluPro: 3.054 ± 0.053	4.325GluGln: 4.325 ± 0.056	3.787GluArg: 3.787 ± 0.05	3.45GluSer: 3.45 ± 0.046	3.766GluThr: 3.766 ± 0.044	4.488GluVal: 4.488 ± 0.053	0.909GluTrp: 0.909 ± 0.022	1.618GluTyr: 1.618 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	3.319PheAla: 3.319 ± 0.053	0.519PheCys: 0.519 ± 0.015	2.304PheAsp: 2.304 ± 0.035	2.279PheGlu: 2.279 ± 0.042	1.669PhePhe: 1.669 ± 0.033	2.927PheGly: 2.927 ± 0.043	0.808PheHis: 0.808 ± 0.026	2.12PheIle: 2.12 ± 0.042	1.169PheLys: 1.169 ± 0.025	4.123PheLeu: 4.123 ± 0.054	0.736PheMet: 0.736 ± 0.02	1.564PheAsn: 1.564 ± 0.031	1.906PhePro: 1.906 ± 0.038	1.862PheGln: 1.862 ± 0.031	2.098PheArg: 2.098 ± 0.03	2.826PheSer: 2.826 ± 0.048	2.325PheThr: 2.325 ± 0.038	2.616PheVal: 2.616 ± 0.04	0.703PheTrp: 0.703 ± 0.021	1.237PheTyr: 1.237 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	5.303GlyAla: 5.303 ± 0.073	0.798GlyCys: 0.798 ± 0.023	3.475GlyAsp: 3.475 ± 0.049	4.237GlyGlu: 4.237 ± 0.053	3.041GlyPhe: 3.041 ± 0.041	4.936GlyGly: 4.936 ± 0.075	1.382GlyHis: 1.382 ± 0.032	4.912GlyIle: 4.912 ± 0.05	3.078GlyLys: 3.078 ± 0.045	7.387GlyLeu: 7.387 ± 0.083	1.785GlyMet: 1.785 ± 0.037	2.82GlyAsn: 2.82 ± 0.07	1.283GlyPro: 1.283 ± 0.026	3.417GlyGln: 3.417 ± 0.052	3.75GlyArg: 3.75 ± 0.051	4.335GlySer: 4.335 ± 0.059	4.041GlyThr: 4.041 ± 0.054	4.909GlyVal: 4.909 ± 0.06	1.207GlyTrp: 1.207 ± 0.027	2.396GlyTyr: 2.396 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	1.433HisAla: 1.433 ± 0.028	0.321HisCys: 0.321 ± 0.015	0.938HisAsp: 0.938 ± 0.024	1.014HisGlu: 1.014 ± 0.021	0.919HisPhe: 0.919 ± 0.021	1.192HisGly: 1.192 ± 0.027	0.781HisHis: 0.781 ± 0.024	1.053HisIle: 1.053 ± 0.022	0.563HisLys: 0.563 ± 0.019	2.677HisLeu: 2.677 ± 0.047	0.283HisMet: 0.283 ± 0.012	0.637HisAsn: 0.637 ± 0.016	1.625HisPro: 1.625 ± 0.034	1.383HisGln: 1.383 ± 0.029	1.402HisArg: 1.402 ± 0.027	1.335HisSer: 1.335 ± 0.025	1.102HisThr: 1.102 ± 0.027	1.013HisVal: 1.013 ± 0.024	0.432HisTrp: 0.432 ± 0.019	0.779HisTyr: 0.779 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.815IleAla: 6.815 ± 0.06	0.644IleCys: 0.644 ± 0.02	3.596IleAsp: 3.596 ± 0.047	3.96IleGlu: 3.96 ± 0.047	2.121IlePhe: 2.121 ± 0.035	4.669IleGly: 4.669 ± 0.049	1.384IleHis: 1.384 ± 0.027	2.64IleIle: 2.64 ± 0.043	1.719IleLys: 1.719 ± 0.034	6.143IleLeu: 6.143 ± 0.063	0.756IleMet: 0.756 ± 0.022	2.01IleAsn: 2.01 ± 0.035	3.43IlePro: 3.43 ± 0.051	3.216IleGln: 3.216 ± 0.041	3.389IleArg: 3.389 ± 0.044	3.555IleSer: 3.555 ± 0.052	3.345IleThr: 3.345 ± 0.046	4.407IleVal: 4.407 ± 0.053	0.837IleTrp: 0.837 ± 0.025	1.649IleTyr: 1.649 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	3.234LysAla: 3.234 ± 0.049	0.207LysCys: 0.207 ± 0.011	1.561LysAsp: 1.561 ± 0.034	1.953LysGlu: 1.953 ± 0.039	1.146LysPhe: 1.146 ± 0.03	2.174LysGly: 2.174 ± 0.041	0.751LysHis: 0.751 ± 0.023	1.943LysIle: 1.943 ± 0.038	1.26LysLys: 1.26 ± 0.032	3.928LysLeu: 3.928 ± 0.048	0.694LysMet: 0.694 ± 0.019	1.145LysAsn: 1.145 ± 0.027	2.073LysPro: 2.073 ± 0.041	2.196LysGln: 2.196 ± 0.035	2.289LysArg: 2.289 ± 0.037	1.949LysSer: 1.949 ± 0.038	2.255LysThr: 2.255 ± 0.044	2.378LysVal: 2.378 ± 0.04	0.394LysTrp: 0.394 ± 0.016	0.864LysTyr: 0.864 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	9.791LeuAla: 9.791 ± 0.074	1.039LeuCys: 1.039 ± 0.025	5.554LeuAsp: 5.554 ± 0.061	7.375LeuGlu: 7.375 ± 0.066	3.942LeuPhe: 3.942 ± 0.056	7.479LeuGly: 7.479 ± 0.076	2.225LeuHis: 2.225 ± 0.035	6.236LeuIle: 6.236 ± 0.073	4.521LeuLys: 4.521 ± 0.057	11.781LeuLeu: 11.781 ± 0.122	2.337LeuMet: 2.337 ± 0.037	4.251LeuAsn: 4.251 ± 0.053	6.094LeuPro: 6.094 ± 0.064	5.864LeuGln: 5.864 ± 0.061	6.3LeuArg: 6.3 ± 0.06	7.748LeuSer: 7.748 ± 0.078	6.525LeuThr: 6.525 ± 0.067	7.338LeuVal: 7.338 ± 0.065	1.61LeuTrp: 1.61 ± 0.037	2.804LeuTyr: 2.804 ± 0.039	0.0LeuXaa: 0.0 ± 0.0
Met	1.991MetAla: 1.991 ± 0.033	0.104MetCys: 0.104 ± 0.008	0.883MetAsp: 0.883 ± 0.022	1.093MetGlu: 1.093 ± 0.029	0.547MetPhe: 0.547 ± 0.018	1.561MetGly: 1.561 ± 0.032	0.383MetHis: 0.383 ± 0.014	1.123MetIle: 1.123 ± 0.028	0.803MetLys: 0.803 ± 0.022	2.038MetLeu: 2.038 ± 0.032	0.535MetMet: 0.535 ± 0.019	0.911MetAsn: 0.911 ± 0.021	1.132MetPro: 1.132 ± 0.022	1.092MetGln: 1.092 ± 0.024	1.076MetArg: 1.076 ± 0.025	1.384MetSer: 1.384 ± 0.034	1.387MetThr: 1.387 ± 0.027	1.474MetVal: 1.474 ± 0.029	0.151MetTrp: 0.151 ± 0.009	0.366MetTyr: 0.366 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.061AsnAla: 3.061 ± 0.043	0.379AsnCys: 0.379 ± 0.016	1.744AsnAsp: 1.744 ± 0.04	1.788AsnGlu: 1.788 ± 0.035	1.425AsnPhe: 1.425 ± 0.029	2.638AsnGly: 2.638 ± 0.039	0.873AsnHis: 0.873 ± 0.022	1.806AsnIle: 1.806 ± 0.033	0.882AsnLys: 0.882 ± 0.022	4.586AsnLeu: 4.586 ± 0.067	0.515AsnMet: 0.515 ± 0.017	1.142AsnAsn: 1.142 ± 0.03	2.787AsnPro: 2.787 ± 0.046	2.281AsnGln: 2.281 ± 0.039	2.372AsnArg: 2.372 ± 0.037	2.151AsnSer: 2.151 ± 0.04	1.819AsnThr: 1.819 ± 0.032	2.151AsnVal: 2.151 ± 0.035	0.66AsnTrp: 0.66 ± 0.022	1.093AsnTyr: 1.093 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	3.97ProAla: 3.97 ± 0.056	0.382ProCys: 0.382 ± 0.015	3.665ProAsp: 3.665 ± 0.049	4.116ProGlu: 4.116 ± 0.061	2.019ProPhe: 2.019 ± 0.034	3.206ProGly: 3.206 ± 0.045	1.089ProHis: 1.089 ± 0.027	3.35ProIle: 3.35 ± 0.044	1.791ProLys: 1.791 ± 0.037	4.959ProLeu: 4.959 ± 0.055	0.965ProMet: 0.965 ± 0.025	2.197ProAsn: 2.197 ± 0.036	2.82ProPro: 2.82 ± 0.053	2.32ProGln: 2.32 ± 0.042	2.013ProArg: 2.013 ± 0.038	3.47ProSer: 3.47 ± 0.052	3.168ProThr: 3.168 ± 0.045	3.533ProVal: 3.533 ± 0.047	0.632ProTrp: 0.632 ± 0.02	1.441ProTyr: 1.441 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	5.419GlnAla: 5.419 ± 0.071	0.41GlnCys: 0.41 ± 0.017	2.355GlnAsp: 2.355 ± 0.035	3.519GlnGlu: 3.519 ± 0.042	2.139GlnPhe: 2.139 ± 0.035	3.437GlnGly: 3.437 ± 0.045	1.126GlnHis: 1.126 ± 0.025	3.427GlnIle: 3.427 ± 0.041	1.817GlnLys: 1.817 ± 0.034	5.756GlnLeu: 5.756 ± 0.071	1.224GlnMet: 1.224 ± 0.026	1.811GlnAsn: 1.811 ± 0.031	3.089GlnPro: 3.089 ± 0.052	4.099GlnGln: 4.099 ± 0.057	3.365GlnArg: 3.365 ± 0.045	3.244GlnSer: 3.244 ± 0.055	3.525GlnThr: 3.525 ± 0.059	4.09GlnVal: 4.09 ± 0.055	0.817GlnTrp: 0.817 ± 0.023	1.271GlnTyr: 1.271 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	3.951ArgAla: 3.951 ± 0.047	0.543ArgCys: 0.543 ± 0.018	2.874ArgAsp: 2.874 ± 0.045	3.501ArgGlu: 3.501 ± 0.053	2.594ArgPhe: 2.594 ± 0.037	3.286ArgGly: 3.286 ± 0.045	1.226ArgHis: 1.226 ± 0.025	3.533ArgIle: 3.533 ± 0.043	1.958ArgLys: 1.958 ± 0.042	7.034ArgLeu: 7.034 ± 0.069	1.267ArgMet: 1.267 ± 0.027	2.032ArgAsn: 2.032 ± 0.032	2.433ArgPro: 2.433 ± 0.045	3.784ArgGln: 3.784 ± 0.054	3.64ArgArg: 3.64 ± 0.055	4.682ArgSer: 4.682 ± 0.052	2.917ArgThr: 2.917 ± 0.041	3.952ArgVal: 3.952 ± 0.049	0.97ArgTrp: 0.97 ± 0.022	1.982ArgTyr: 1.982 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	4.89SerAla: 4.89 ± 0.054	0.571SerCys: 0.571 ± 0.02	3.373SerAsp: 3.373 ± 0.049	3.851SerGlu: 3.851 ± 0.052	2.509SerPhe: 2.509 ± 0.038	4.77SerGly: 4.77 ± 0.072	1.399SerHis: 1.399 ± 0.028	3.742SerIle: 3.742 ± 0.047	1.952SerLys: 1.952 ± 0.034	7.237SerLeu: 7.237 ± 0.065	1.27SerMet: 1.27 ± 0.025	2.313SerAsn: 2.313 ± 0.035	3.979SerPro: 3.979 ± 0.055	3.356SerGln: 3.356 ± 0.04	3.482SerArg: 3.482 ± 0.045	4.53SerSer: 4.53 ± 0.067	3.46SerThr: 3.46 ± 0.053	4.164SerVal: 4.164 ± 0.05	0.906SerTrp: 0.906 ± 0.021	1.687SerTyr: 1.687 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	5.197ThrAla: 5.197 ± 0.054	0.481ThrCys: 0.481 ± 0.019	2.846ThrAsp: 2.846 ± 0.043	3.286ThrGlu: 3.286 ± 0.039	2.145ThrPhe: 2.145 ± 0.032	4.327ThrGly: 4.327 ± 0.06	1.222ThrHis: 1.222 ± 0.026	3.795ThrIle: 3.795 ± 0.057	1.565ThrLys: 1.565 ± 0.03	6.8ThrLeu: 6.8 ± 0.071	0.861ThrMet: 0.861 ± 0.024	1.852ThrAsn: 1.852 ± 0.034	3.518ThrPro: 3.518 ± 0.046	2.827ThrGln: 2.827 ± 0.042	2.583ThrArg: 2.583 ± 0.037	3.235ThrSer: 3.235 ± 0.047	3.258ThrThr: 3.258 ± 0.051	4.36ThrVal: 4.36 ± 0.05	0.766ThrTrp: 0.766 ± 0.024	1.651ThrTyr: 1.651 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	6.239ValAla: 6.239 ± 0.061	0.712ValCys: 0.712 ± 0.018	3.566ValAsp: 3.566 ± 0.045	4.701ValGlu: 4.701 ± 0.051	2.616ValPhe: 2.616 ± 0.036	4.896ValGly: 4.896 ± 0.06	1.235ValHis: 1.235 ± 0.03	4.143ValIle: 4.143 ± 0.051	2.559ValLys: 2.559 ± 0.04	7.403ValLeu: 7.403 ± 0.068	1.623ValMet: 1.623 ± 0.028	2.637ValAsn: 2.637 ± 0.041	3.166ValPro: 3.166 ± 0.046	3.364ValGln: 3.364 ± 0.046	3.876ValArg: 3.876 ± 0.048	4.359ValSer: 4.359 ± 0.056	3.703ValThr: 3.703 ± 0.051	5.099ValVal: 5.099 ± 0.061	1.015ValTrp: 1.015 ± 0.027	1.84ValTyr: 1.84 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.087TrpAla: 1.087 ± 0.027	0.144TrpCys: 0.144 ± 0.009	0.728TrpAsp: 0.728 ± 0.03	0.876TrpGlu: 0.876 ± 0.024	0.666TrpPhe: 0.666 ± 0.02	0.994TrpGly: 0.994 ± 0.026	0.361TrpHis: 0.361 ± 0.016	0.939TrpIle: 0.939 ± 0.025	0.545TrpLys: 0.545 ± 0.016	1.992TrpLeu: 1.992 ± 0.034	0.395TrpMet: 0.395 ± 0.016	0.677TrpAsn: 0.677 ± 0.021	0.186TrpPro: 0.186 ± 0.01	1.261TrpGln: 1.261 ± 0.026	0.908TrpArg: 0.908 ± 0.022	0.932TrpSer: 0.932 ± 0.027	0.721TrpThr: 0.721 ± 0.021	1.077TrpVal: 1.077 ± 0.026	0.25TrpTrp: 0.25 ± 0.013	0.381TrpTyr: 0.381 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.267TyrAla: 2.267 ± 0.038	0.347TyrCys: 0.347 ± 0.016	1.521TyrAsp: 1.521 ± 0.034	1.721TyrGlu: 1.721 ± 0.032	1.2TyrPhe: 1.2 ± 0.027	2.183TyrGly: 2.183 ± 0.043	0.672TyrHis: 0.672 ± 0.018	1.316TyrIle: 1.316 ± 0.028	0.796TyrLys: 0.796 ± 0.022	3.347TyrLeu: 3.347 ± 0.04	0.405TyrMet: 0.405 ± 0.016	0.889TyrAsn: 0.889 ± 0.022	1.592TyrPro: 1.592 ± 0.031	1.724TyrGln: 1.724 ± 0.031	2.1TyrArg: 2.1 ± 0.032	1.731TyrSer: 1.731 ± 0.034	1.459TyrThr: 1.459 ± 0.029	1.732TyrVal: 1.732 ± 0.03	0.526TyrTrp: 0.526 ± 0.018	0.918TyrTyr: 0.918 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6213 proteins (1872383 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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