Proteus phage Myduc

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Chaseviridae; Cleopatravirinae; Myducvirus; Proteus virus Myduc

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6T7I0|A0A5J6T7I0_9CAUD Uncharacterized protein OS=Proteus phage Myduc OX=2650874 GN=CPT_Myduc_015 PE=4 SV=1
MM1 pKa = 7.83SEE3 pKa = 4.8LEE5 pKa = 4.32LLLFASVKK13 pKa = 10.03IDD15 pKa = 3.68FKK17 pKa = 11.57KK18 pKa = 10.39EE19 pKa = 3.27DD20 pKa = 3.88AYY22 pKa = 11.11NTFAGYY28 pKa = 10.32DD29 pKa = 3.55DD30 pKa = 4.76EE31 pKa = 5.46MLAEE35 pKa = 4.18YY36 pKa = 10.06YY37 pKa = 10.55YY38 pKa = 10.85KK39 pKa = 9.59YY40 pKa = 9.1THH42 pKa = 6.82GEE44 pKa = 3.83LSKK47 pKa = 10.87EE48 pKa = 4.28LILDD52 pKa = 3.93AVYY55 pKa = 10.25EE56 pKa = 4.04AVEE59 pKa = 4.4EE60 pKa = 4.36IEE62 pKa = 4.45NSNCAIDD69 pKa = 3.97FDD71 pKa = 5.06KK72 pKa = 11.21EE73 pKa = 3.92

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6T783|A0A5J6T783_9CAUD ZP domain-containing protein OS=Proteus phage Myduc OX=2650874 GN=CPT_Myduc_020 PE=4 SV=1
MM1 pKa = 8.01RR2 pKa = 11.84KK3 pKa = 8.41PQNSYY8 pKa = 11.14SRR10 pKa = 11.84FKK12 pKa = 10.2EE13 pKa = 3.88WASMHH18 pKa = 6.56EE19 pKa = 4.74DD20 pKa = 3.06ANQSNEE26 pKa = 3.68RR27 pKa = 11.84VTRR30 pKa = 11.84TSGTMLGNGGFIMRR44 pKa = 11.84STTRR48 pKa = 11.84SSKK51 pKa = 10.88LGIGDD56 pKa = 3.89KK57 pKa = 10.36IFRR60 pKa = 11.84AILWVIVIYY69 pKa = 9.93FCVFIAINN77 pKa = 3.35

Molecular weight: 8.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

16146

31

1289

204.4

22.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.825 ± 0.296

1.307 ± 0.158

6.045 ± 0.211

6.862 ± 0.249

4.212 ± 0.223

7.451 ± 0.417

1.778 ± 0.097

6.367 ± 0.263

7.054 ± 0.324

7.599 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.738 ± 0.186

5.147 ± 0.264

3.561 ± 0.161

3.803 ± 0.265

4.633 ± 0.21

7.017 ± 0.302

5.76 ± 0.239

6.887 ± 0.252

1.412 ± 0.115

3.543 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski