Amino acid dipepetide frequency for Proteus phage Myduc

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.557AlaAla: 0.557 ± 0.231
1.053AlaCys: 1.053 ± 0.259
3.964AlaAsp: 3.964 ± 0.411
4.893AlaGlu: 4.893 ± 0.643
3.159AlaPhe: 3.159 ± 0.373
5.451AlaGly: 5.451 ± 0.756
1.363AlaHis: 1.363 ± 0.289
5.946AlaIle: 5.946 ± 0.529
4.46AlaLys: 4.46 ± 0.492
5.636AlaLeu: 5.636 ± 0.651
2.292AlaMet: 2.292 ± 0.336
3.778AlaAsn: 3.778 ± 0.453
1.61AlaPro: 1.61 ± 0.319
2.911AlaGln: 2.911 ± 0.741
3.531AlaArg: 3.531 ± 0.517
5.079AlaSer: 5.079 ± 0.524
4.336AlaThr: 4.336 ± 0.513
5.698AlaVal: 5.698 ± 0.76
0.496AlaTrp: 0.496 ± 0.156
1.982AlaTyr: 1.982 ± 0.313
0.0AlaXaa: 0.0 ± 0.0
Cys
0.743CysAla: 0.743 ± 0.187
0.186CysCys: 0.186 ± 0.101
1.053CysAsp: 1.053 ± 0.278
0.991CysGlu: 0.991 ± 0.218
0.867CysPhe: 0.867 ± 0.208
0.805CysGly: 0.805 ± 0.231
0.31CysHis: 0.31 ± 0.124
0.557CysIle: 0.557 ± 0.165
1.239CysLys: 1.239 ± 0.309
0.929CysLeu: 0.929 ± 0.291
0.31CysMet: 0.31 ± 0.131
0.619CysAsn: 0.619 ± 0.197
0.31CysPro: 0.31 ± 0.156
0.743CysGln: 0.743 ± 0.22
0.619CysArg: 0.619 ± 0.211
0.743CysSer: 0.743 ± 0.212
0.557CysThr: 0.557 ± 0.192
1.053CysVal: 1.053 ± 0.276
0.31CysTrp: 0.31 ± 0.138
0.124CysTyr: 0.124 ± 0.087
0.0CysXaa: 0.0 ± 0.0
Asp
4.398AspAla: 4.398 ± 0.46
1.115AspCys: 1.115 ± 0.263
2.663AspAsp: 2.663 ± 0.484
3.902AspGlu: 3.902 ± 0.524
2.911AspPhe: 2.911 ± 0.386
6.008AspGly: 6.008 ± 0.532
0.929AspHis: 0.929 ± 0.192
3.221AspIle: 3.221 ± 0.42
4.831AspLys: 4.831 ± 0.496
3.902AspLeu: 3.902 ± 0.536
1.858AspMet: 1.858 ± 0.317
3.592AspAsn: 3.592 ± 0.568
1.92AspPro: 1.92 ± 0.35
1.115AspGln: 1.115 ± 0.231
2.539AspArg: 2.539 ± 0.441
5.389AspSer: 5.389 ± 0.772
2.663AspThr: 2.663 ± 0.345
4.15AspVal: 4.15 ± 0.637
1.053AspTrp: 1.053 ± 0.259
2.292AspTyr: 2.292 ± 0.499
0.0AspXaa: 0.0 ± 0.0
Glu
6.194GluAla: 6.194 ± 0.851
0.681GluCys: 0.681 ± 0.208
4.707GluAsp: 4.707 ± 0.493
5.946GluGlu: 5.946 ± 0.679
3.035GluPhe: 3.035 ± 0.423
2.478GluGly: 2.478 ± 0.465
1.61GluHis: 1.61 ± 0.284
5.451GluIle: 5.451 ± 0.61
4.15GluLys: 4.15 ± 0.552
5.327GluLeu: 5.327 ± 0.798
1.858GluMet: 1.858 ± 0.31
3.159GluAsn: 3.159 ± 0.372
1.61GluPro: 1.61 ± 0.354
2.787GluGln: 2.787 ± 0.52
3.221GluArg: 3.221 ± 0.49
3.654GluSer: 3.654 ± 0.374
3.716GluThr: 3.716 ± 0.511
5.389GluVal: 5.389 ± 0.597
1.053GluTrp: 1.053 ± 0.224
2.601GluTyr: 2.601 ± 0.362
0.0GluXaa: 0.0 ± 0.0
Phe
2.849PheAla: 2.849 ± 0.337
0.743PheCys: 0.743 ± 0.196
3.654PheAsp: 3.654 ± 0.467
2.849PheGlu: 2.849 ± 0.428
0.867PhePhe: 0.867 ± 0.241
3.035PheGly: 3.035 ± 0.369
0.991PheHis: 0.991 ± 0.242
2.539PheIle: 2.539 ± 0.411
4.15PheLys: 4.15 ± 0.651
2.416PheLeu: 2.416 ± 0.388
1.363PheMet: 1.363 ± 0.288
2.478PheAsn: 2.478 ± 0.383
1.734PhePro: 1.734 ± 0.376
0.434PheGln: 0.434 ± 0.163
2.044PheArg: 2.044 ± 0.325
3.283PheSer: 3.283 ± 0.467
2.106PheThr: 2.106 ± 0.429
2.601PheVal: 2.601 ± 0.374
0.619PheTrp: 0.619 ± 0.161
1.363PheTyr: 1.363 ± 0.3
0.0PheXaa: 0.0 ± 0.0
Gly
5.389GlyAla: 5.389 ± 0.802
0.743GlyCys: 0.743 ± 0.201
4.088GlyAsp: 4.088 ± 0.73
5.327GlyGlu: 5.327 ± 0.573
3.283GlyPhe: 3.283 ± 0.45
6.07GlyGly: 6.07 ± 0.841
0.991GlyHis: 0.991 ± 0.318
4.522GlyIle: 4.522 ± 0.494
6.38GlyLys: 6.38 ± 0.78
5.946GlyLeu: 5.946 ± 0.614
2.292GlyMet: 2.292 ± 0.423
3.778GlyAsn: 3.778 ± 0.615
1.239GlyPro: 1.239 ± 0.241
2.23GlyGln: 2.23 ± 0.354
3.531GlyArg: 3.531 ± 0.705
5.017GlySer: 5.017 ± 0.593
4.46GlyThr: 4.46 ± 0.572
5.203GlyVal: 5.203 ± 0.638
1.301GlyTrp: 1.301 ± 0.319
2.725GlyTyr: 2.725 ± 0.352
0.0GlyXaa: 0.0 ± 0.0
His
0.991HisAla: 0.991 ± 0.285
0.372HisCys: 0.372 ± 0.142
0.929HisAsp: 0.929 ± 0.227
1.115HisGlu: 1.115 ± 0.32
0.496HisPhe: 0.496 ± 0.179
0.681HisGly: 0.681 ± 0.188
0.124HisHis: 0.124 ± 0.091
0.991HisIle: 0.991 ± 0.24
1.053HisLys: 1.053 ± 0.244
1.053HisLeu: 1.053 ± 0.227
0.372HisMet: 0.372 ± 0.137
1.548HisAsn: 1.548 ± 0.268
1.053HisPro: 1.053 ± 0.193
0.681HisGln: 0.681 ± 0.186
0.867HisArg: 0.867 ± 0.206
1.363HisSer: 1.363 ± 0.295
0.991HisThr: 0.991 ± 0.277
1.548HisVal: 1.548 ± 0.316
0.434HisTrp: 0.434 ± 0.139
1.115HisTyr: 1.115 ± 0.321
0.0HisXaa: 0.0 ± 0.0
Ile
4.274IleAla: 4.274 ± 0.558
0.991IleCys: 0.991 ± 0.29
3.902IleAsp: 3.902 ± 0.474
3.221IleGlu: 3.221 ± 0.447
2.663IlePhe: 2.663 ± 0.486
4.274IleGly: 4.274 ± 0.498
0.743IleHis: 0.743 ± 0.182
3.716IleIle: 3.716 ± 0.503
4.583IleLys: 4.583 ± 0.552
5.327IleLeu: 5.327 ± 0.614
1.734IleMet: 1.734 ± 0.326
3.592IleAsn: 3.592 ± 0.456
3.221IlePro: 3.221 ± 0.522
2.478IleGln: 2.478 ± 0.357
3.592IleArg: 3.592 ± 0.527
4.274IleSer: 4.274 ± 0.481
4.398IleThr: 4.398 ± 0.596
3.592IleVal: 3.592 ± 0.448
1.053IleTrp: 1.053 ± 0.236
2.044IleTyr: 2.044 ± 0.448
0.0IleXaa: 0.0 ± 0.0
Lys
4.769LysAla: 4.769 ± 0.564
0.867LysCys: 0.867 ± 0.196
4.769LysAsp: 4.769 ± 0.537
5.203LysGlu: 5.203 ± 0.62
2.787LysPhe: 2.787 ± 0.488
4.707LysGly: 4.707 ± 0.497
1.548LysHis: 1.548 ± 0.281
4.893LysIle: 4.893 ± 0.664
4.274LysLys: 4.274 ± 0.612
5.884LysLeu: 5.884 ± 0.627
2.354LysMet: 2.354 ± 0.349
2.725LysAsn: 2.725 ± 0.467
2.354LysPro: 2.354 ± 0.361
3.407LysGln: 3.407 ± 0.448
3.592LysArg: 3.592 ± 0.5
4.026LysSer: 4.026 ± 0.493
4.522LysThr: 4.522 ± 0.532
4.583LysVal: 4.583 ± 0.51
0.929LysTrp: 0.929 ± 0.175
2.354LysTyr: 2.354 ± 0.351
0.0LysXaa: 0.0 ± 0.0
Leu
5.822LeuAla: 5.822 ± 0.624
1.053LeuCys: 1.053 ± 0.256
3.531LeuAsp: 3.531 ± 0.391
6.132LeuGlu: 6.132 ± 0.692
2.973LeuPhe: 2.973 ± 0.372
5.574LeuGly: 5.574 ± 0.767
1.363LeuHis: 1.363 ± 0.31
3.902LeuIle: 3.902 ± 0.568
6.194LeuLys: 6.194 ± 0.748
5.141LeuLeu: 5.141 ± 0.658
1.239LeuMet: 1.239 ± 0.271
4.893LeuAsn: 4.893 ± 0.512
2.911LeuPro: 2.911 ± 0.469
2.787LeuGln: 2.787 ± 0.359
3.345LeuArg: 3.345 ± 0.442
6.442LeuSer: 6.442 ± 0.617
4.088LeuThr: 4.088 ± 0.434
5.265LeuVal: 5.265 ± 0.485
1.115LeuTrp: 1.115 ± 0.292
2.23LeuTyr: 2.23 ± 0.325
0.0LeuXaa: 0.0 ± 0.0
Met
2.787MetAla: 2.787 ± 0.552
0.186MetCys: 0.186 ± 0.121
2.044MetAsp: 2.044 ± 0.372
1.425MetGlu: 1.425 ± 0.258
1.239MetPhe: 1.239 ± 0.275
1.487MetGly: 1.487 ± 0.406
0.557MetHis: 0.557 ± 0.172
1.301MetIle: 1.301 ± 0.272
1.92MetLys: 1.92 ± 0.399
2.168MetLeu: 2.168 ± 0.313
0.619MetMet: 0.619 ± 0.258
1.115MetAsn: 1.115 ± 0.227
0.619MetPro: 0.619 ± 0.174
1.796MetGln: 1.796 ± 0.453
1.115MetArg: 1.115 ± 0.237
2.354MetSer: 2.354 ± 0.317
1.548MetThr: 1.548 ± 0.316
1.363MetVal: 1.363 ± 0.284
0.619MetTrp: 0.619 ± 0.2
1.115MetTyr: 1.115 ± 0.249
0.0MetXaa: 0.0 ± 0.0
Asn
3.654AsnAla: 3.654 ± 0.498
0.681AsnCys: 0.681 ± 0.242
2.416AsnAsp: 2.416 ± 0.364
2.911AsnGlu: 2.911 ± 0.353
2.539AsnPhe: 2.539 ± 0.5
3.407AsnGly: 3.407 ± 0.438
0.743AsnHis: 0.743 ± 0.197
3.097AsnIle: 3.097 ± 0.477
3.407AsnLys: 3.407 ± 0.48
4.336AsnLeu: 4.336 ± 0.491
1.115AsnMet: 1.115 ± 0.257
2.849AsnAsn: 2.849 ± 0.443
3.283AsnPro: 3.283 ± 0.429
1.734AsnGln: 1.734 ± 0.291
2.663AsnArg: 2.663 ± 0.365
3.221AsnSer: 3.221 ± 0.611
3.221AsnThr: 3.221 ± 0.341
3.716AsnVal: 3.716 ± 0.464
0.743AsnTrp: 0.743 ± 0.191
1.734AsnTyr: 1.734 ± 0.363
0.0AsnXaa: 0.0 ± 0.0
Pro
2.787ProAla: 2.787 ± 0.352
0.31ProCys: 0.31 ± 0.132
2.478ProAsp: 2.478 ± 0.468
3.159ProGlu: 3.159 ± 0.378
1.177ProPhe: 1.177 ± 0.268
2.354ProGly: 2.354 ± 0.34
0.496ProHis: 0.496 ± 0.183
1.858ProIle: 1.858 ± 0.34
2.168ProLys: 2.168 ± 0.364
2.725ProLeu: 2.725 ± 0.364
0.681ProMet: 0.681 ± 0.206
2.601ProAsn: 2.601 ± 0.378
0.743ProPro: 0.743 ± 0.181
1.177ProGln: 1.177 ± 0.298
1.425ProArg: 1.425 ± 0.32
2.23ProSer: 2.23 ± 0.395
2.416ProThr: 2.416 ± 0.491
3.407ProVal: 3.407 ± 0.473
0.496ProTrp: 0.496 ± 0.16
0.929ProTyr: 0.929 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
2.601GlnAla: 2.601 ± 0.475
0.496GlnCys: 0.496 ± 0.152
1.982GlnAsp: 1.982 ± 0.38
2.539GlnGlu: 2.539 ± 0.381
1.548GlnPhe: 1.548 ± 0.266
2.911GlnGly: 2.911 ± 0.393
0.681GlnHis: 0.681 ± 0.236
2.725GlnIle: 2.725 ± 0.354
1.548GlnLys: 1.548 ± 0.372
2.911GlnLeu: 2.911 ± 0.524
1.61GlnMet: 1.61 ± 0.349
1.858GlnAsn: 1.858 ± 0.289
1.672GlnPro: 1.672 ± 0.358
2.168GlnGln: 2.168 ± 0.935
1.92GlnArg: 1.92 ± 0.396
2.044GlnSer: 2.044 ± 0.319
2.168GlnThr: 2.168 ± 0.398
2.292GlnVal: 2.292 ± 0.31
0.434GlnTrp: 0.434 ± 0.146
1.92GlnTyr: 1.92 ± 0.321
0.0GlnXaa: 0.0 ± 0.0
Arg
3.221ArgAla: 3.221 ± 0.465
0.619ArgCys: 0.619 ± 0.224
2.292ArgAsp: 2.292 ± 0.394
3.159ArgGlu: 3.159 ± 0.395
2.292ArgPhe: 2.292 ± 0.463
3.531ArgGly: 3.531 ± 0.854
0.991ArgHis: 0.991 ± 0.257
3.035ArgIle: 3.035 ± 0.355
2.539ArgLys: 2.539 ± 0.377
3.531ArgLeu: 3.531 ± 0.412
1.115ArgMet: 1.115 ± 0.238
1.734ArgAsn: 1.734 ± 0.282
1.796ArgPro: 1.796 ± 0.275
2.106ArgGln: 2.106 ± 0.331
1.982ArgArg: 1.982 ± 0.4
2.663ArgSer: 2.663 ± 0.337
2.478ArgThr: 2.478 ± 0.426
3.964ArgVal: 3.964 ± 0.523
0.991ArgTrp: 0.991 ± 0.254
2.292ArgTyr: 2.292 ± 0.352
0.0ArgXaa: 0.0 ± 0.0
Ser
4.522SerAla: 4.522 ± 0.618
0.619SerCys: 0.619 ± 0.222
5.636SerAsp: 5.636 ± 0.913
3.531SerGlu: 3.531 ± 0.48
3.84SerPhe: 3.84 ± 0.607
6.875SerGly: 6.875 ± 0.574
1.177SerHis: 1.177 ± 0.228
4.274SerIle: 4.274 ± 0.597
5.451SerLys: 5.451 ± 0.488
5.884SerLeu: 5.884 ± 0.586
2.044SerMet: 2.044 ± 0.329
2.787SerAsn: 2.787 ± 0.424
2.539SerPro: 2.539 ± 0.337
2.663SerGln: 2.663 ± 0.603
2.787SerArg: 2.787 ± 0.383
4.583SerSer: 4.583 ± 0.537
4.336SerThr: 4.336 ± 0.628
3.902SerVal: 3.902 ± 0.506
0.496SerTrp: 0.496 ± 0.15
2.23SerTyr: 2.23 ± 0.363
0.0SerXaa: 0.0 ± 0.0
Thr
4.15ThrAla: 4.15 ± 0.519
0.557ThrCys: 0.557 ± 0.206
2.478ThrAsp: 2.478 ± 0.351
3.592ThrGlu: 3.592 ± 0.365
2.044ThrPhe: 2.044 ± 0.373
5.265ThrGly: 5.265 ± 0.586
1.239ThrHis: 1.239 ± 0.272
3.716ThrIle: 3.716 ± 0.401
4.026ThrLys: 4.026 ± 0.542
4.893ThrLeu: 4.893 ± 0.674
0.929ThrMet: 0.929 ± 0.22
2.601ThrAsn: 2.601 ± 0.388
2.416ThrPro: 2.416 ± 0.409
2.416ThrGln: 2.416 ± 0.331
2.601ThrArg: 2.601 ± 0.461
4.893ThrSer: 4.893 ± 0.649
3.221ThrThr: 3.221 ± 0.446
4.088ThrVal: 4.088 ± 0.449
0.557ThrTrp: 0.557 ± 0.16
1.92ThrTyr: 1.92 ± 0.327
0.0ThrXaa: 0.0 ± 0.0
Val
6.008ValAla: 6.008 ± 0.617
1.177ValCys: 1.177 ± 0.307
4.583ValAsp: 4.583 ± 0.517
4.955ValGlu: 4.955 ± 0.698
2.601ValPhe: 2.601 ± 0.479
5.513ValGly: 5.513 ± 0.608
1.177ValHis: 1.177 ± 0.222
4.893ValIle: 4.893 ± 0.72
4.522ValLys: 4.522 ± 0.528
3.964ValLeu: 3.964 ± 0.505
1.672ValMet: 1.672 ± 0.396
2.787ValAsn: 2.787 ± 0.449
2.663ValPro: 2.663 ± 0.429
2.354ValGln: 2.354 ± 0.326
3.035ValArg: 3.035 ± 0.431
4.707ValSer: 4.707 ± 0.569
3.964ValThr: 3.964 ± 0.454
5.079ValVal: 5.079 ± 0.66
0.929ValTrp: 0.929 ± 0.203
2.292ValTyr: 2.292 ± 0.47
0.0ValXaa: 0.0 ± 0.0
Trp
0.681TrpAla: 0.681 ± 0.204
0.434TrpCys: 0.434 ± 0.143
0.929TrpAsp: 0.929 ± 0.227
1.672TrpGlu: 1.672 ± 0.332
0.619TrpPhe: 0.619 ± 0.189
0.743TrpGly: 0.743 ± 0.212
0.062TrpHis: 0.062 ± 0.056
0.496TrpIle: 0.496 ± 0.15
0.929TrpLys: 0.929 ± 0.241
1.301TrpLeu: 1.301 ± 0.268
0.31TrpMet: 0.31 ± 0.153
0.929TrpAsn: 0.929 ± 0.268
0.0TrpPro: 0.0 ± 0.0
0.867TrpGln: 0.867 ± 0.207
0.496TrpArg: 0.496 ± 0.139
1.239TrpSer: 1.239 ± 0.291
0.681TrpThr: 0.681 ± 0.203
0.619TrpVal: 0.619 ± 0.202
0.124TrpTrp: 0.124 ± 0.078
0.991TrpTyr: 0.991 ± 0.253
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.858TyrAla: 1.858 ± 0.248
0.186TyrCys: 0.186 ± 0.101
2.354TyrAsp: 2.354 ± 0.386
2.106TyrGlu: 2.106 ± 0.276
1.177TyrPhe: 1.177 ± 0.318
3.345TyrGly: 3.345 ± 0.561
0.681TyrHis: 0.681 ± 0.213
2.539TyrIle: 2.539 ± 0.538
2.725TyrLys: 2.725 ± 0.412
2.725TyrLeu: 2.725 ± 0.427
1.548TyrMet: 1.548 ± 0.299
2.044TyrAsn: 2.044 ± 0.305
1.92TyrPro: 1.92 ± 0.354
1.177TyrGln: 1.177 ± 0.26
1.425TyrArg: 1.425 ± 0.304
2.973TyrSer: 2.973 ± 0.406
1.734TyrThr: 1.734 ± 0.287
1.363TyrVal: 1.363 ± 0.249
0.372TyrTrp: 0.372 ± 0.162
1.177TyrTyr: 1.177 ± 0.325
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (16146 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski