Neglecta sp. 59

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Neglecta; unclassified Neglecta

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3248 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N9PRD5|A0A6N9PRD5_9FIRM DUF1273 family protein OS=Neglecta sp. 59 OX=2304573 GN=D1644_07595 PE=4 SV=1
MM1 pKa = 6.99LTVMVAFWLTGCGSSVSQEE20 pKa = 3.91EE21 pKa = 3.98LDD23 pKa = 4.53ALNKK27 pKa = 9.85QNSEE31 pKa = 3.98LQEE34 pKa = 4.05QIDD37 pKa = 3.96ALEE40 pKa = 4.58SRR42 pKa = 11.84LSATPTPSPLVQEE55 pKa = 5.29GYY57 pKa = 10.9NNFVQRR63 pKa = 11.84YY64 pKa = 7.48NEE66 pKa = 4.28YY67 pKa = 10.04IGEE70 pKa = 4.68ADD72 pKa = 3.79TPDD75 pKa = 4.41KK76 pKa = 11.22IEE78 pKa = 4.61MLSEE82 pKa = 4.76DD83 pKa = 4.03NNSFQPTTWTKK94 pKa = 10.08ATICFNEE101 pKa = 5.2DD102 pKa = 2.77GTLDD106 pKa = 3.6NVLFAAEE113 pKa = 4.48PDD115 pKa = 3.82TAVEE119 pKa = 4.46DD120 pKa = 3.07IAYY123 pKa = 9.43FFKK126 pKa = 10.47TVNAMTVVAYY136 pKa = 10.16GIDD139 pKa = 3.34EE140 pKa = 4.59SMTEE144 pKa = 3.94NQSVDD149 pKa = 3.66FRR151 pKa = 11.84MDD153 pKa = 2.72IMQNYY158 pKa = 8.29FFDD161 pKa = 4.45DD162 pKa = 3.47QALKK166 pKa = 9.32STYY169 pKa = 7.14EE170 pKa = 3.73TRR172 pKa = 11.84LNQFNYY178 pKa = 10.74SFFILSGNVCMSIEE192 pKa = 4.18VAA194 pKa = 4.11

Molecular weight: 21.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N9PXT1|A0A6N9PXT1_9FIRM Uncharacterized protein OS=Neglecta sp. 59 OX=2304573 GN=D1644_12740 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84GKK4 pKa = 10.69LFRR7 pKa = 11.84LRR9 pKa = 11.84GKK11 pKa = 10.17LLQLLQRR18 pKa = 11.84LLRR21 pKa = 11.84VRR23 pKa = 11.84RR24 pKa = 11.84EE25 pKa = 3.95LLFLLQRR32 pKa = 11.84MFRR35 pKa = 11.84LLQLFRR41 pKa = 11.84RR42 pKa = 11.84LRR44 pKa = 11.84LWVQRR49 pKa = 11.84LLWEE53 pKa = 4.26KK54 pKa = 9.86LHH56 pKa = 6.06WGLRR60 pKa = 11.84HH61 pKa = 5.93RR62 pKa = 11.84LLRR65 pKa = 11.84RR66 pKa = 11.84LRR68 pKa = 11.84GKK70 pKa = 10.03LFRR73 pKa = 11.84MQRR76 pKa = 11.84KK77 pKa = 8.54LLRR80 pKa = 11.84PVRR83 pKa = 11.84GLFRR87 pKa = 11.84MWQRR91 pKa = 11.84VRR93 pKa = 11.84IRR95 pKa = 11.84LFRR98 pKa = 11.84LRR100 pKa = 11.84GLLWQCHH107 pKa = 4.77RR108 pKa = 11.84RR109 pKa = 11.84VQRR112 pKa = 11.84LFQRR116 pKa = 11.84LQKK119 pKa = 10.26LYY121 pKa = 11.2RR122 pKa = 11.84MM123 pKa = 4.63

Molecular weight: 16.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3248

0

3248

899311

23

1778

276.9

30.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.082 ± 0.053

1.671 ± 0.022

5.185 ± 0.038

7.381 ± 0.051

4.119 ± 0.031

7.851 ± 0.049

1.718 ± 0.017

5.633 ± 0.039

5.621 ± 0.045

9.834 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.804 ± 0.023

3.41 ± 0.029

4.276 ± 0.039

3.773 ± 0.025

5.495 ± 0.044

5.705 ± 0.033

5.106 ± 0.039

6.744 ± 0.035

1.049 ± 0.016

3.544 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski