Amino acid dipepetide frequency for Kangiella sp. HZ709

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.778AlaAla: 6.778 ± 0.129
0.807AlaCys: 0.807 ± 0.036
4.364AlaAsp: 4.364 ± 0.097
5.607AlaGlu: 5.607 ± 0.104
3.387AlaPhe: 3.387 ± 0.074
5.666AlaGly: 5.666 ± 0.114
1.458AlaHis: 1.458 ± 0.052
6.339AlaIle: 6.339 ± 0.103
5.667AlaLys: 5.667 ± 0.106
8.219AlaLeu: 8.219 ± 0.119
2.141AlaMet: 2.141 ± 0.064
4.027AlaAsn: 4.027 ± 0.088
2.518AlaPro: 2.518 ± 0.067
3.282AlaGln: 3.282 ± 0.08
3.195AlaArg: 3.195 ± 0.076
5.09AlaSer: 5.09 ± 0.085
4.169AlaThr: 4.169 ± 0.084
5.15AlaVal: 5.15 ± 0.101
0.88AlaTrp: 0.88 ± 0.035
2.448AlaTyr: 2.448 ± 0.074
0.0AlaXaa: 0.0 ± 0.0
Cys
0.557CysAla: 0.557 ± 0.033
0.095CysCys: 0.095 ± 0.013
0.526CysAsp: 0.526 ± 0.033
0.505CysGlu: 0.505 ± 0.031
0.324CysPhe: 0.324 ± 0.02
0.708CysGly: 0.708 ± 0.038
0.238CysHis: 0.238 ± 0.016
0.458CysIle: 0.458 ± 0.026
0.444CysLys: 0.444 ± 0.028
0.852CysLeu: 0.852 ± 0.035
0.169CysMet: 0.169 ± 0.015
0.332CysAsn: 0.332 ± 0.024
0.389CysPro: 0.389 ± 0.028
0.381CysGln: 0.381 ± 0.024
0.359CysArg: 0.359 ± 0.024
0.655CysSer: 0.655 ± 0.032
0.387CysThr: 0.387 ± 0.024
0.463CysVal: 0.463 ± 0.029
0.082CysTrp: 0.082 ± 0.009
0.287CysTyr: 0.287 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
4.337AspAla: 4.337 ± 0.103
0.478AspCys: 0.478 ± 0.029
3.109AspAsp: 3.109 ± 0.103
3.734AspGlu: 3.734 ± 0.086
2.845AspPhe: 2.845 ± 0.061
4.047AspGly: 4.047 ± 0.12
0.922AspHis: 0.922 ± 0.036
3.968AspIle: 3.968 ± 0.072
3.923AspLys: 3.923 ± 0.084
5.799AspLeu: 5.799 ± 0.109
1.383AspMet: 1.383 ± 0.046
2.782AspAsn: 2.782 ± 0.083
2.24AspPro: 2.24 ± 0.075
1.91AspGln: 1.91 ± 0.059
2.116AspArg: 2.116 ± 0.055
3.732AspSer: 3.732 ± 0.069
2.707AspThr: 2.707 ± 0.09
3.354AspVal: 3.354 ± 0.081
0.931AspTrp: 0.931 ± 0.039
2.422AspTyr: 2.422 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
5.312GluAla: 5.312 ± 0.087
0.389GluCys: 0.389 ± 0.025
3.071GluAsp: 3.071 ± 0.078
4.327GluGlu: 4.327 ± 0.119
2.729GluPhe: 2.729 ± 0.059
3.436GluGly: 3.436 ± 0.085
1.445GluHis: 1.445 ± 0.044
4.421GluIle: 4.421 ± 0.076
4.533GluLys: 4.533 ± 0.098
7.387GluLeu: 7.387 ± 0.128
1.615GluMet: 1.615 ± 0.057
3.001GluAsn: 3.001 ± 0.072
2.019GluPro: 2.019 ± 0.059
3.938GluGln: 3.938 ± 0.097
2.993GluArg: 2.993 ± 0.077
4.161GluSer: 4.161 ± 0.088
3.316GluThr: 3.316 ± 0.068
4.396GluVal: 4.396 ± 0.086
0.757GluTrp: 0.757 ± 0.033
2.075GluTyr: 2.075 ± 0.069
0.0GluXaa: 0.0 ± 0.0
Phe
3.475PheAla: 3.475 ± 0.068
0.409PheCys: 0.409 ± 0.027
2.932PheAsp: 2.932 ± 0.067
2.921PheGlu: 2.921 ± 0.073
1.959PhePhe: 1.959 ± 0.057
3.18PheGly: 3.18 ± 0.069
0.771PheHis: 0.771 ± 0.033
3.092PheIle: 3.092 ± 0.073
2.67PheLys: 2.67 ± 0.057
3.694PheLeu: 3.694 ± 0.088
0.995PheMet: 0.995 ± 0.039
2.444PheAsn: 2.444 ± 0.065
1.473PhePro: 1.473 ± 0.045
1.383PheGln: 1.383 ± 0.043
1.447PheArg: 1.447 ± 0.053
3.489PheSer: 3.489 ± 0.071
2.258PheThr: 2.258 ± 0.058
2.714PheVal: 2.714 ± 0.06
0.535PheTrp: 0.535 ± 0.027
1.596PheTyr: 1.596 ± 0.049
0.0PheXaa: 0.0 ± 0.0
Gly
5.149GlyAla: 5.149 ± 0.107
0.65GlyCys: 0.65 ± 0.03
3.664GlyAsp: 3.664 ± 0.078
3.908GlyGlu: 3.908 ± 0.085
3.519GlyPhe: 3.519 ± 0.072
5.004GlyGly: 5.004 ± 0.158
1.525GlyHis: 1.525 ± 0.046
4.777GlyIle: 4.777 ± 0.094
4.248GlyLys: 4.248 ± 0.1
6.671GlyLeu: 6.671 ± 0.109
1.716GlyMet: 1.716 ± 0.054
2.944GlyAsn: 2.944 ± 0.127
1.637GlyPro: 1.637 ± 0.047
2.639GlyGln: 2.639 ± 0.073
2.815GlyArg: 2.815 ± 0.074
4.19GlySer: 4.19 ± 0.107
3.455GlyThr: 3.455 ± 0.093
4.74GlyVal: 4.74 ± 0.093
0.901GlyTrp: 0.901 ± 0.036
2.519GlyTyr: 2.519 ± 0.067
0.0GlyXaa: 0.0 ± 0.0
His
1.28HisAla: 1.28 ± 0.046
0.26HisCys: 0.26 ± 0.019
1.01HisAsp: 1.01 ± 0.037
1.012HisGlu: 1.012 ± 0.04
1.02HisPhe: 1.02 ± 0.039
1.377HisGly: 1.377 ± 0.05
0.565HisHis: 0.565 ± 0.023
1.322HisIle: 1.322 ± 0.044
1.272HisLys: 1.272 ± 0.041
2.158HisLeu: 2.158 ± 0.062
0.432HisMet: 0.432 ± 0.025
0.881HisAsn: 0.881 ± 0.036
1.031HisPro: 1.031 ± 0.037
1.085HisGln: 1.085 ± 0.036
0.94HisArg: 0.94 ± 0.042
1.325HisSer: 1.325 ± 0.049
0.873HisThr: 0.873 ± 0.038
0.976HisVal: 0.976 ± 0.037
0.345HisTrp: 0.345 ± 0.022
0.857HisTyr: 0.857 ± 0.038
0.0HisXaa: 0.0 ± 0.0
Ile
6.173IleAla: 6.173 ± 0.126
0.621IleCys: 0.621 ± 0.031
4.585IleAsp: 4.585 ± 0.073
5.272IleGlu: 5.272 ± 0.111
2.673IlePhe: 2.673 ± 0.081
4.871IleGly: 4.871 ± 0.094
1.306IleHis: 1.306 ± 0.041
4.543IleIle: 4.543 ± 0.103
4.549IleLys: 4.549 ± 0.094
5.983IleLeu: 5.983 ± 0.125
1.318IleMet: 1.318 ± 0.051
3.672IleAsn: 3.672 ± 0.075
2.854IlePro: 2.854 ± 0.07
2.559IleGln: 2.559 ± 0.071
2.725IleArg: 2.725 ± 0.053
5.08IleSer: 5.08 ± 0.087
3.86IleThr: 3.86 ± 0.087
4.081IleVal: 4.081 ± 0.094
0.681IleTrp: 0.681 ± 0.036
2.026IleTyr: 2.026 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
5.793LysAla: 5.793 ± 0.111
0.325LysCys: 0.325 ± 0.031
3.693LysAsp: 3.693 ± 0.071
4.37LysGlu: 4.37 ± 0.085
2.111LysPhe: 2.111 ± 0.056
3.835LysGly: 3.835 ± 0.074
1.351LysHis: 1.351 ± 0.051
4.13LysIle: 4.13 ± 0.092
4.598LysLys: 4.598 ± 0.118
6.183LysLeu: 6.183 ± 0.116
1.552LysMet: 1.552 ± 0.049
3.094LysAsn: 3.094 ± 0.066
2.755LysPro: 2.755 ± 0.071
3.061LysGln: 3.061 ± 0.071
2.915LysArg: 2.915 ± 0.067
4.199LysSer: 4.199 ± 0.09
3.598LysThr: 3.598 ± 0.079
4.635LysVal: 4.635 ± 0.082
0.599LysTrp: 0.599 ± 0.028
1.819LysTyr: 1.819 ± 0.048
0.0LysXaa: 0.0 ± 0.0
Leu
8.847LeuAla: 8.847 ± 0.146
0.757LeuCys: 0.757 ± 0.033
5.784LeuAsp: 5.784 ± 0.096
6.744LeuGlu: 6.744 ± 0.121
4.144LeuPhe: 4.144 ± 0.091
6.467LeuGly: 6.467 ± 0.103
1.757LeuHis: 1.757 ± 0.057
7.139LeuIle: 7.139 ± 0.132
6.429LeuLys: 6.429 ± 0.111
10.271LeuLeu: 10.271 ± 0.191
2.575LeuMet: 2.575 ± 0.065
4.955LeuAsn: 4.955 ± 0.094
4.071LeuPro: 4.071 ± 0.076
3.727LeuGln: 3.727 ± 0.076
3.788LeuArg: 3.788 ± 0.074
7.477LeuSer: 7.477 ± 0.11
5.376LeuThr: 5.376 ± 0.091
6.642LeuVal: 6.642 ± 0.109
1.083LeuTrp: 1.083 ± 0.039
2.727LeuTyr: 2.727 ± 0.058
0.0LeuXaa: 0.0 ± 0.0
Met
2.328MetAla: 2.328 ± 0.072
0.132MetCys: 0.132 ± 0.012
1.228MetAsp: 1.228 ± 0.047
1.273MetGlu: 1.273 ± 0.043
0.787MetPhe: 0.787 ± 0.035
1.631MetGly: 1.631 ± 0.047
0.508MetHis: 0.508 ± 0.026
1.54MetIle: 1.54 ± 0.059
1.554MetLys: 1.554 ± 0.044
2.33MetLeu: 2.33 ± 0.066
0.699MetMet: 0.699 ± 0.03
1.114MetAsn: 1.114 ± 0.04
1.1MetPro: 1.1 ± 0.041
1.269MetGln: 1.269 ± 0.039
1.087MetArg: 1.087 ± 0.036
1.789MetSer: 1.789 ± 0.044
1.352MetThr: 1.352 ± 0.046
1.687MetVal: 1.687 ± 0.053
0.202MetTrp: 0.202 ± 0.018
0.467MetTyr: 0.467 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.657AsnAla: 3.657 ± 0.082
0.387AsnCys: 0.387 ± 0.025
2.721AsnAsp: 2.721 ± 0.07
2.625AsnGlu: 2.625 ± 0.072
2.19AsnPhe: 2.19 ± 0.06
3.363AsnGly: 3.363 ± 0.1
1.068AsnHis: 1.068 ± 0.037
3.254AsnIle: 3.254 ± 0.076
3.323AsnLys: 3.323 ± 0.072
4.671AsnLeu: 4.671 ± 0.089
1.034AsnMet: 1.034 ± 0.036
2.583AsnAsn: 2.583 ± 0.07
2.338AsnPro: 2.338 ± 0.058
2.591AsnGln: 2.591 ± 0.054
2.079AsnArg: 2.079 ± 0.066
3.366AsnSer: 3.366 ± 0.081
2.428AsnThr: 2.428 ± 0.086
2.527AsnVal: 2.527 ± 0.065
0.663AsnTrp: 0.663 ± 0.027
1.712AsnTyr: 1.712 ± 0.049
0.0AsnXaa: 0.0 ± 0.0
Pro
2.794ProAla: 2.794 ± 0.068
0.264ProCys: 0.264 ± 0.021
2.1ProAsp: 2.1 ± 0.049
3.079ProGlu: 3.079 ± 0.072
1.712ProPhe: 1.712 ± 0.05
2.233ProGly: 2.233 ± 0.07
0.689ProHis: 0.689 ± 0.03
2.68ProIle: 2.68 ± 0.057
2.417ProLys: 2.417 ± 0.063
3.619ProLeu: 3.619 ± 0.074
0.959ProMet: 0.959 ± 0.039
1.951ProAsn: 1.951 ± 0.052
1.046ProPro: 1.046 ± 0.041
1.437ProGln: 1.437 ± 0.051
1.275ProArg: 1.275 ± 0.05
2.361ProSer: 2.361 ± 0.055
2.068ProThr: 2.068 ± 0.059
2.604ProVal: 2.604 ± 0.067
0.394ProTrp: 0.394 ± 0.026
1.264ProTyr: 1.264 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
3.858GlnAla: 3.858 ± 0.085
0.268GlnCys: 0.268 ± 0.021
2.195GlnAsp: 2.195 ± 0.057
2.948GlnGlu: 2.948 ± 0.073
1.778GlnPhe: 1.778 ± 0.05
2.583GlnGly: 2.583 ± 0.056
0.929GlnHis: 0.929 ± 0.036
2.804GlnIle: 2.804 ± 0.064
2.771GlnLys: 2.771 ± 0.068
5.046GlnLeu: 5.046 ± 0.107
1.066GlnMet: 1.066 ± 0.04
1.787GlnAsn: 1.787 ± 0.053
1.427GlnPro: 1.427 ± 0.043
2.593GlnGln: 2.593 ± 0.087
1.912GlnArg: 1.912 ± 0.059
2.857GlnSer: 2.857 ± 0.072
2.113GlnThr: 2.113 ± 0.056
2.956GlnVal: 2.956 ± 0.068
0.635GlnTrp: 0.635 ± 0.031
1.454GlnTyr: 1.454 ± 0.058
0.0GlnXaa: 0.0 ± 0.0
Arg
2.977ArgAla: 2.977 ± 0.06
0.324ArgCys: 0.324 ± 0.021
2.252ArgAsp: 2.252 ± 0.053
2.61ArgGlu: 2.61 ± 0.06
2.03ArgPhe: 2.03 ± 0.05
2.447ArgGly: 2.447 ± 0.063
0.953ArgHis: 0.953 ± 0.042
2.808ArgIle: 2.808 ± 0.061
2.67ArgLys: 2.67 ± 0.069
4.288ArgLeu: 4.288 ± 0.085
1.114ArgMet: 1.114 ± 0.042
1.961ArgAsn: 1.961 ± 0.054
1.43ArgPro: 1.43 ± 0.045
1.954ArgGln: 1.954 ± 0.054
1.961ArgArg: 1.961 ± 0.062
2.427ArgSer: 2.427 ± 0.064
1.829ArgThr: 1.829 ± 0.054
2.917ArgVal: 2.917 ± 0.074
0.523ArgTrp: 0.523 ± 0.029
1.652ArgTyr: 1.652 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
4.954SerAla: 4.954 ± 0.083
0.603SerCys: 0.603 ± 0.031
3.904SerAsp: 3.904 ± 0.082
4.424SerGlu: 4.424 ± 0.082
3.173SerPhe: 3.173 ± 0.07
4.758SerGly: 4.758 ± 0.106
1.364SerHis: 1.364 ± 0.047
4.985SerIle: 4.985 ± 0.091
4.387SerLys: 4.387 ± 0.082
7.017SerLeu: 7.017 ± 0.113
1.637SerMet: 1.637 ± 0.052
3.525SerAsn: 3.525 ± 0.087
2.316SerPro: 2.316 ± 0.063
2.941SerGln: 2.941 ± 0.08
2.785SerArg: 2.785 ± 0.063
4.83SerSer: 4.83 ± 0.116
3.203SerThr: 3.203 ± 0.097
4.214SerVal: 4.214 ± 0.076
0.896SerTrp: 0.896 ± 0.044
2.331SerTyr: 2.331 ± 0.076
0.0SerXaa: 0.0 ± 0.0
Thr
4.251ThrAla: 4.251 ± 0.093
0.328ThrCys: 0.328 ± 0.021
3.064ThrAsp: 3.064 ± 0.088
3.212ThrGlu: 3.212 ± 0.073
2.139ThrPhe: 2.139 ± 0.066
3.855ThrGly: 3.855 ± 0.085
1.006ThrHis: 1.006 ± 0.041
3.73ThrIle: 3.73 ± 0.073
2.767ThrLys: 2.767 ± 0.066
5.213ThrLeu: 5.213 ± 0.094
1.029ThrMet: 1.029 ± 0.04
2.535ThrAsn: 2.535 ± 0.066
2.269ThrPro: 2.269 ± 0.06
2.116ThrGln: 2.116 ± 0.06
1.976ThrArg: 1.976 ± 0.063
3.501ThrSer: 3.501 ± 0.091
2.613ThrThr: 2.613 ± 0.1
3.637ThrVal: 3.637 ± 0.094
0.553ThrTrp: 0.553 ± 0.035
1.585ThrTyr: 1.585 ± 0.096
0.0ThrXaa: 0.0 ± 0.0
Val
5.701ValAla: 5.701 ± 0.108
0.617ValCys: 0.617 ± 0.028
3.949ValAsp: 3.949 ± 0.07
4.41ValGlu: 4.41 ± 0.081
2.678ValPhe: 2.678 ± 0.064
4.317ValGly: 4.317 ± 0.101
1.085ValHis: 1.085 ± 0.042
4.811ValIle: 4.811 ± 0.094
3.958ValLys: 3.958 ± 0.069
5.998ValLeu: 5.998 ± 0.106
1.691ValMet: 1.691 ± 0.055
3.101ValAsn: 3.101 ± 0.069
2.309ValPro: 2.309 ± 0.062
2.033ValGln: 2.033 ± 0.056
2.535ValArg: 2.535 ± 0.057
4.562ValSer: 4.562 ± 0.07
3.859ValThr: 3.859 ± 0.087
4.321ValVal: 4.321 ± 0.085
0.694ValTrp: 0.694 ± 0.032
1.799ValTyr: 1.799 ± 0.053
0.0ValXaa: 0.0 ± 0.0
Trp
0.775TrpAla: 0.775 ± 0.035
0.11TrpCys: 0.11 ± 0.012
0.635TrpAsp: 0.635 ± 0.031
0.547TrpGlu: 0.547 ± 0.028
0.62TrpPhe: 0.62 ± 0.033
0.682TrpGly: 0.682 ± 0.03
0.331TrpHis: 0.331 ± 0.023
0.763TrpIle: 0.763 ± 0.032
0.613TrpLys: 0.613 ± 0.029
1.721TrpLeu: 1.721 ± 0.058
0.312TrpMet: 0.312 ± 0.02
0.524TrpAsn: 0.524 ± 0.026
0.383TrpPro: 0.383 ± 0.023
0.846TrpGln: 0.846 ± 0.038
0.557TrpArg: 0.557 ± 0.03
0.786TrpSer: 0.786 ± 0.035
0.489TrpThr: 0.489 ± 0.025
0.742TrpVal: 0.742 ± 0.03
0.167TrpTrp: 0.167 ± 0.016
0.464TrpTyr: 0.464 ± 0.03
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.198TyrAla: 2.198 ± 0.054
0.387TyrCys: 0.387 ± 0.022
1.955TyrAsp: 1.955 ± 0.156
1.791TyrGlu: 1.791 ± 0.06
1.622TyrPhe: 1.622 ± 0.049
2.211TyrGly: 2.211 ± 0.06
0.749TyrHis: 0.749 ± 0.033
1.87TyrIle: 1.87 ± 0.048
1.822TyrLys: 1.822 ± 0.05
3.638TyrLeu: 3.638 ± 0.079
0.64TyrMet: 0.64 ± 0.028
1.366TyrAsn: 1.366 ± 0.066
1.339TyrPro: 1.339 ± 0.035
2.235TyrGln: 2.235 ± 0.064
1.667TyrArg: 1.667 ± 0.053
2.371TyrSer: 2.371 ± 0.069
1.427TyrThr: 1.427 ± 0.068
1.674TyrVal: 1.674 ± 0.056
0.546TyrTrp: 0.546 ± 0.029
1.265TyrTyr: 1.265 ± 0.06
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2214 proteins (734385 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski