Pseudomonas sp. C27(2019)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6QAF9|A0A5J6QAF9_9PSED Glutamate-1-semialdehyde 2 1-aminomutase OS=Pseudomonas sp. C27(2019) OX=2604941 GN=hemL PE=3 SV=1
MM1 pKa = 6.95ATNVVMVSGTVFGAAEE17 pKa = 4.26DD18 pKa = 4.33VAHH21 pKa = 6.86HH22 pKa = 6.84AAQILTEE29 pKa = 4.45AGLNVIYY36 pKa = 10.3KK37 pKa = 10.11PRR39 pKa = 11.84FTYY42 pKa = 10.39PEE44 pKa = 4.36LLEE47 pKa = 4.71LEE49 pKa = 4.45PDD51 pKa = 3.19ALLFVTSTTGMGEE64 pKa = 4.09LPEE67 pKa = 4.23NLQPLMDD74 pKa = 4.52TLLEE78 pKa = 4.18QPPSWSGVLGGVIALGDD95 pKa = 3.46ACYY98 pKa = 10.99GEE100 pKa = 5.14DD101 pKa = 3.42FCAGGEE107 pKa = 4.2AVRR110 pKa = 11.84EE111 pKa = 4.23LYY113 pKa = 10.5SALGVVEE120 pKa = 4.37LQDD123 pKa = 3.91MLRR126 pKa = 11.84LDD128 pKa = 3.66ASEE131 pKa = 4.31TVSPEE136 pKa = 3.45SDD138 pKa = 3.3AEE140 pKa = 3.84PWLAEE145 pKa = 4.0FAVSLSLL152 pKa = 4.59

Molecular weight: 16.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6QC48|A0A5J6QC48_9PSED Uncharacterized protein OS=Pseudomonas sp. C27(2019) OX=2604941 GN=FXF61_12285 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.47RR12 pKa = 11.84ARR14 pKa = 11.84NHH16 pKa = 5.29GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVIKK32 pKa = 10.2RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2792

0

2792

900516

25

2405

322.5

35.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.216 ± 0.051

1.006 ± 0.016

5.406 ± 0.031

5.948 ± 0.041

3.713 ± 0.032

6.873 ± 0.043

2.362 ± 0.024

5.616 ± 0.036

4.1 ± 0.038

11.459 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.451 ± 0.022

3.515 ± 0.033

4.232 ± 0.028

5.313 ± 0.047

5.714 ± 0.038

6.169 ± 0.03

5.017 ± 0.028

6.881 ± 0.042

1.337 ± 0.019

2.672 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski