Amino acid dipepetide frequency for Pseudomonas sp. C27(2019)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.065AlaAla: 11.065 ± 0.151
1.128AlaCys: 1.128 ± 0.036
5.741AlaAsp: 5.741 ± 0.08
6.695AlaGlu: 6.695 ± 0.091
3.44AlaPhe: 3.44 ± 0.065
7.422AlaGly: 7.422 ± 0.103
2.168AlaHis: 2.168 ± 0.051
5.588AlaIle: 5.588 ± 0.078
4.009AlaLys: 4.009 ± 0.071
12.564AlaLeu: 12.564 ± 0.133
2.73AlaMet: 2.73 ± 0.058
3.053AlaAsn: 3.053 ± 0.057
3.588AlaPro: 3.588 ± 0.069
6.25AlaGln: 6.25 ± 0.115
5.529AlaArg: 5.529 ± 0.078
5.685AlaSer: 5.685 ± 0.081
4.489AlaThr: 4.489 ± 0.076
7.193AlaVal: 7.193 ± 0.102
1.408AlaTrp: 1.408 ± 0.037
2.419AlaTyr: 2.419 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
1.09CysAla: 1.09 ± 0.04
0.141CysCys: 0.141 ± 0.016
0.499CysAsp: 0.499 ± 0.025
0.521CysGlu: 0.521 ± 0.021
0.353CysPhe: 0.353 ± 0.019
0.84CysGly: 0.84 ± 0.037
0.306CysHis: 0.306 ± 0.024
0.561CysIle: 0.561 ± 0.026
0.345CysLys: 0.345 ± 0.02
1.026CysLeu: 1.026 ± 0.037
0.24CysMet: 0.24 ± 0.016
0.328CysAsn: 0.328 ± 0.019
0.483CysPro: 0.483 ± 0.024
0.462CysGln: 0.462 ± 0.023
0.497CysArg: 0.497 ± 0.028
0.735CysSer: 0.735 ± 0.028
0.493CysThr: 0.493 ± 0.028
0.732CysVal: 0.732 ± 0.031
0.132CysTrp: 0.132 ± 0.011
0.277CysTyr: 0.277 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
5.412AspAla: 5.412 ± 0.085
0.573AspCys: 0.573 ± 0.028
3.022AspAsp: 3.022 ± 0.066
3.613AspGlu: 3.613 ± 0.065
2.217AspPhe: 2.217 ± 0.052
3.849AspGly: 3.849 ± 0.074
1.026AspHis: 1.026 ± 0.035
3.46AspIle: 3.46 ± 0.066
2.348AspLys: 2.348 ± 0.053
5.842AspLeu: 5.842 ± 0.088
1.3AspMet: 1.3 ± 0.047
1.987AspAsn: 1.987 ± 0.045
2.285AspPro: 2.285 ± 0.05
2.131AspGln: 2.131 ± 0.053
2.572AspArg: 2.572 ± 0.054
3.291AspSer: 3.291 ± 0.058
2.705AspThr: 2.705 ± 0.06
3.791AspVal: 3.791 ± 0.073
0.928AspTrp: 0.928 ± 0.03
1.711AspTyr: 1.711 ± 0.049
0.0AspXaa: 0.0 ± 0.0
Glu
5.471GluAla: 5.471 ± 0.086
0.462GluCys: 0.462 ± 0.024
2.728GluAsp: 2.728 ± 0.063
3.416GluGlu: 3.416 ± 0.076
2.093GluPhe: 2.093 ± 0.048
3.437GluGly: 3.437 ± 0.069
2.011GluHis: 2.011 ± 0.043
3.139GluIle: 3.139 ± 0.072
2.764GluLys: 2.764 ± 0.06
7.136GluLeu: 7.136 ± 0.089
1.461GluMet: 1.461 ± 0.044
1.92GluAsn: 1.92 ± 0.048
2.302GluPro: 2.302 ± 0.055
4.941GluGln: 4.941 ± 0.095
4.375GluArg: 4.375 ± 0.075
2.916GluSer: 2.916 ± 0.06
2.693GluThr: 2.693 ± 0.056
4.007GluVal: 4.007 ± 0.069
0.706GluTrp: 0.706 ± 0.031
1.505GluTyr: 1.505 ± 0.049
0.0GluXaa: 0.0 ± 0.0
Phe
4.077PheAla: 4.077 ± 0.06
0.442PheCys: 0.442 ± 0.023
2.359PheAsp: 2.359 ± 0.056
2.002PheGlu: 2.002 ± 0.053
1.566PhePhe: 1.566 ± 0.049
2.733PheGly: 2.733 ± 0.066
0.665PheHis: 0.665 ± 0.027
2.261PheIle: 2.261 ± 0.051
1.618PheLys: 1.618 ± 0.045
3.491PheLeu: 3.491 ± 0.064
0.922PheMet: 0.922 ± 0.032
1.634PheAsn: 1.634 ± 0.042
1.339PhePro: 1.339 ± 0.04
1.192PheGln: 1.192 ± 0.036
1.602PheArg: 1.602 ± 0.04
2.946PheSer: 2.946 ± 0.06
2.1PheThr: 2.1 ± 0.049
2.554PheVal: 2.554 ± 0.054
0.473PheTrp: 0.473 ± 0.025
1.149PheTyr: 1.149 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
5.923GlyAla: 5.923 ± 0.09
0.922GlyCys: 0.922 ± 0.032
3.598GlyAsp: 3.598 ± 0.069
3.967GlyGlu: 3.967 ± 0.07
3.106GlyPhe: 3.106 ± 0.066
4.674GlyGly: 4.674 ± 0.103
1.688GlyHis: 1.688 ± 0.044
4.152GlyIle: 4.152 ± 0.074
3.271GlyLys: 3.271 ± 0.065
7.766GlyLeu: 7.766 ± 0.103
2.044GlyMet: 2.044 ± 0.054
2.119GlyAsn: 2.119 ± 0.052
1.952GlyPro: 1.952 ± 0.05
3.357GlyGln: 3.357 ± 0.068
4.018GlyArg: 4.018 ± 0.07
4.144GlySer: 4.144 ± 0.068
3.326GlyThr: 3.326 ± 0.073
5.46GlyVal: 5.46 ± 0.104
1.049GlyTrp: 1.049 ± 0.043
2.19GlyTyr: 2.19 ± 0.052
0.0GlyXaa: 0.0 ± 0.0
His
2.391HisAla: 2.391 ± 0.055
0.34HisCys: 0.34 ± 0.02
1.206HisAsp: 1.206 ± 0.039
1.087HisGlu: 1.087 ± 0.038
1.062HisPhe: 1.062 ± 0.032
1.662HisGly: 1.662 ± 0.052
0.546HisHis: 0.546 ± 0.027
1.376HisIle: 1.376 ± 0.035
0.831HisLys: 0.831 ± 0.031
2.56HisLeu: 2.56 ± 0.058
0.571HisMet: 0.571 ± 0.026
0.905HisAsn: 0.905 ± 0.033
1.336HisPro: 1.336 ± 0.037
1.046HisGln: 1.046 ± 0.037
1.215HisArg: 1.215 ± 0.034
1.556HisSer: 1.556 ± 0.041
1.225HisThr: 1.225 ± 0.038
1.45HisVal: 1.45 ± 0.043
0.442HisTrp: 0.442 ± 0.023
0.814HisTyr: 0.814 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
6.285IleAla: 6.285 ± 0.095
0.504IleCys: 0.504 ± 0.022
3.877IleAsp: 3.877 ± 0.064
4.192IleGlu: 4.192 ± 0.072
1.798IlePhe: 1.798 ± 0.048
4.316IleGly: 4.316 ± 0.077
1.078IleHis: 1.078 ± 0.036
3.077IleIle: 3.077 ± 0.065
2.564IleLys: 2.564 ± 0.053
4.903IleLeu: 4.903 ± 0.087
1.12IleMet: 1.12 ± 0.036
2.481IleAsn: 2.481 ± 0.058
2.289IlePro: 2.289 ± 0.053
2.061IleGln: 2.061 ± 0.046
2.918IleArg: 2.918 ± 0.061
3.96IleSer: 3.96 ± 0.083
3.096IleThr: 3.096 ± 0.058
3.578IleVal: 3.578 ± 0.071
0.6IleTrp: 0.6 ± 0.032
1.465IleTyr: 1.465 ± 0.043
0.0IleXaa: 0.0 ± 0.0
Lys
4.029LysAla: 4.029 ± 0.073
0.247LysCys: 0.247 ± 0.018
2.19LysAsp: 2.19 ± 0.059
2.29LysGlu: 2.29 ± 0.054
1.014LysPhe: 1.014 ± 0.035
2.69LysGly: 2.69 ± 0.067
1.104LysHis: 1.104 ± 0.042
2.304LysIle: 2.304 ± 0.057
2.245LysLys: 2.245 ± 0.064
4.018LysLeu: 4.018 ± 0.085
1.026LysMet: 1.026 ± 0.035
1.731LysAsn: 1.731 ± 0.047
2.088LysPro: 2.088 ± 0.053
2.461LysGln: 2.461 ± 0.055
2.607LysArg: 2.607 ± 0.058
2.283LysSer: 2.283 ± 0.058
2.416LysThr: 2.416 ± 0.051
2.898LysVal: 2.898 ± 0.055
0.371LysTrp: 0.371 ± 0.022
0.992LysTyr: 0.992 ± 0.039
0.0LysXaa: 0.0 ± 0.0
Leu
12.651LeuAla: 12.651 ± 0.145
1.183LeuCys: 1.183 ± 0.042
6.342LeuAsp: 6.342 ± 0.088
6.65LeuGlu: 6.65 ± 0.095
4.14LeuPhe: 4.14 ± 0.075
7.608LeuGly: 7.608 ± 0.109
2.452LeuHis: 2.452 ± 0.05
6.168LeuIle: 6.168 ± 0.098
4.795LeuLys: 4.795 ± 0.078
13.321LeuLeu: 13.321 ± 0.186
2.625LeuMet: 2.625 ± 0.056
4.282LeuAsn: 4.282 ± 0.083
5.651LeuPro: 5.651 ± 0.066
5.688LeuGln: 5.688 ± 0.1
6.584LeuArg: 6.584 ± 0.094
7.669LeuSer: 7.669 ± 0.098
5.897LeuThr: 5.897 ± 0.08
6.907LeuVal: 6.907 ± 0.09
1.335LeuTrp: 1.335 ± 0.04
2.648LeuTyr: 2.648 ± 0.06
0.0LeuXaa: 0.0 ± 0.0
Met
2.452MetAla: 2.452 ± 0.064
0.191MetCys: 0.191 ± 0.015
1.178MetAsp: 1.178 ± 0.039
0.934MetGlu: 0.934 ± 0.029
0.77MetPhe: 0.77 ± 0.031
1.665MetGly: 1.665 ± 0.049
0.682MetHis: 0.682 ± 0.026
1.256MetIle: 1.256 ± 0.042
0.906MetLys: 0.906 ± 0.034
3.075MetLeu: 3.075 ± 0.063
0.574MetMet: 0.574 ± 0.028
0.894MetAsn: 0.894 ± 0.032
1.325MetPro: 1.325 ± 0.032
1.497MetGln: 1.497 ± 0.039
1.63MetArg: 1.63 ± 0.045
1.858MetSer: 1.858 ± 0.044
1.416MetThr: 1.416 ± 0.041
1.524MetVal: 1.524 ± 0.05
0.204MetTrp: 0.204 ± 0.014
0.481MetTyr: 0.481 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
3.369AsnAla: 3.369 ± 0.072
0.342AsnCys: 0.342 ± 0.018
1.876AsnAsp: 1.876 ± 0.051
1.903AsnGlu: 1.903 ± 0.052
1.186AsnPhe: 1.186 ± 0.038
2.305AsnGly: 2.305 ± 0.06
0.823AsnHis: 0.823 ± 0.033
2.215AsnIle: 2.215 ± 0.053
1.587AsnLys: 1.587 ± 0.046
3.645AsnLeu: 3.645 ± 0.07
0.782AsnMet: 0.782 ± 0.03
1.53AsnAsn: 1.53 ± 0.057
2.088AsnPro: 2.088 ± 0.053
1.735AsnGln: 1.735 ± 0.06
1.868AsnArg: 1.868 ± 0.052
2.25AsnSer: 2.25 ± 0.051
2.03AsnThr: 2.03 ± 0.05
2.129AsnVal: 2.129 ± 0.045
0.466AsnTrp: 0.466 ± 0.024
1.017AsnTyr: 1.017 ± 0.034
0.0AsnXaa: 0.0 ± 0.0
Pro
4.399ProAla: 4.399 ± 0.081
0.334ProCys: 0.334 ± 0.019
2.513ProAsp: 2.513 ± 0.052
3.429ProGlu: 3.429 ± 0.075
1.656ProPhe: 1.656 ± 0.044
2.656ProGly: 2.656 ± 0.058
0.978ProHis: 0.978 ± 0.035
2.224ProIle: 2.224 ± 0.041
1.66ProLys: 1.66 ± 0.038
4.827ProLeu: 4.827 ± 0.078
1.078ProMet: 1.078 ± 0.034
1.488ProAsn: 1.488 ± 0.045
1.393ProPro: 1.393 ± 0.044
1.959ProGln: 1.959 ± 0.048
1.931ProArg: 1.931 ± 0.045
2.641ProSer: 2.641 ± 0.05
1.996ProThr: 1.996 ± 0.051
3.315ProVal: 3.315 ± 0.062
0.633ProTrp: 0.633 ± 0.027
1.208ProTyr: 1.208 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
6.343GlnAla: 6.343 ± 0.114
0.38GlnCys: 0.38 ± 0.019
2.169GlnAsp: 2.169 ± 0.052
2.571GlnGlu: 2.571 ± 0.06
1.545GlnPhe: 1.545 ± 0.035
3.589GlnGly: 3.589 ± 0.088
1.74GlnHis: 1.74 ± 0.049
2.467GlnIle: 2.467 ± 0.051
1.639GlnLys: 1.639 ± 0.046
6.602GlnLeu: 6.602 ± 0.124
1.234GlnMet: 1.234 ± 0.036
1.365GlnAsn: 1.365 ± 0.042
2.463GlnPro: 2.463 ± 0.056
4.324GlnGln: 4.324 ± 0.12
3.937GlnArg: 3.937 ± 0.083
2.662GlnSer: 2.662 ± 0.068
2.405GlnThr: 2.405 ± 0.057
3.745GlnVal: 3.745 ± 0.07
0.806GlnTrp: 0.806 ± 0.031
1.142GlnTyr: 1.142 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
5.107ArgAla: 5.107 ± 0.074
0.557ArgCys: 0.557 ± 0.025
2.963ArgAsp: 2.963 ± 0.064
3.669ArgGlu: 3.669 ± 0.065
2.57ArgPhe: 2.57 ± 0.058
3.325ArgGly: 3.325 ± 0.07
1.398ArgHis: 1.398 ± 0.041
3.472ArgIle: 3.472 ± 0.059
2.383ArgLys: 2.383 ± 0.056
7.063ArgLeu: 7.063 ± 0.101
1.497ArgMet: 1.497 ± 0.041
2.026ArgAsn: 2.026 ± 0.044
2.119ArgPro: 2.119 ± 0.05
2.924ArgGln: 2.924 ± 0.057
3.273ArgArg: 3.273 ± 0.063
3.375ArgSer: 3.375 ± 0.062
2.567ArgThr: 2.567 ± 0.056
3.946ArgVal: 3.946 ± 0.066
0.875ArgTrp: 0.875 ± 0.031
2.032ArgTyr: 2.032 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
6.434SerAla: 6.434 ± 0.087
0.571SerCys: 0.571 ± 0.026
3.139SerAsp: 3.139 ± 0.057
3.561SerGlu: 3.561 ± 0.07
2.418SerPhe: 2.418 ± 0.045
4.806SerGly: 4.806 ± 0.085
1.344SerHis: 1.344 ± 0.038
3.623SerIle: 3.623 ± 0.063
2.573SerLys: 2.573 ± 0.061
6.723SerLeu: 6.723 ± 0.093
1.631SerMet: 1.631 ± 0.038
2.304SerAsn: 2.304 ± 0.061
2.274SerPro: 2.274 ± 0.058
2.728SerGln: 2.728 ± 0.061
3.23SerArg: 3.23 ± 0.065
4.212SerSer: 4.212 ± 0.086
3.258SerThr: 3.258 ± 0.063
4.291SerVal: 4.291 ± 0.07
0.865SerTrp: 0.865 ± 0.03
1.701SerTyr: 1.701 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
5.325ThrAla: 5.325 ± 0.078
0.463ThrCys: 0.463 ± 0.025
2.851ThrAsp: 2.851 ± 0.049
2.982ThrGlu: 2.982 ± 0.049
1.803ThrPhe: 1.803 ± 0.046
4.023ThrGly: 4.023 ± 0.078
1.162ThrHis: 1.162 ± 0.037
2.523ThrIle: 2.523 ± 0.056
1.398ThrLys: 1.398 ± 0.044
6.516ThrLeu: 6.516 ± 0.09
0.955ThrMet: 0.955 ± 0.028
1.407ThrAsn: 1.407 ± 0.049
2.681ThrPro: 2.681 ± 0.057
2.579ThrGln: 2.579 ± 0.057
2.727ThrArg: 2.727 ± 0.062
2.74ThrSer: 2.74 ± 0.062
2.586ThrThr: 2.586 ± 0.064
3.651ThrVal: 3.651 ± 0.069
0.611ThrTrp: 0.611 ± 0.026
1.184ThrTyr: 1.184 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
6.858ValAla: 6.858 ± 0.098
0.767ValCys: 0.767 ± 0.031
3.821ValAsp: 3.821 ± 0.062
4.229ValGlu: 4.229 ± 0.067
2.771ValPhe: 2.771 ± 0.069
4.523ValGly: 4.523 ± 0.086
1.489ValHis: 1.489 ± 0.039
4.234ValIle: 4.234 ± 0.071
2.611ValLys: 2.611 ± 0.058
8.193ValLeu: 8.193 ± 0.125
1.821ValMet: 1.821 ± 0.05
2.339ValAsn: 2.339 ± 0.059
2.771ValPro: 2.771 ± 0.057
3.017ValGln: 3.017 ± 0.059
3.924ValArg: 3.924 ± 0.071
4.262ValSer: 4.262 ± 0.065
3.591ValThr: 3.591 ± 0.06
5.112ValVal: 5.112 ± 0.09
0.822ValTrp: 0.822 ± 0.033
1.657ValTyr: 1.657 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
0.945TrpAla: 0.945 ± 0.032
0.175TrpCys: 0.175 ± 0.014
0.559TrpAsp: 0.559 ± 0.023
0.502TrpGlu: 0.502 ± 0.022
0.512TrpPhe: 0.512 ± 0.028
0.761TrpGly: 0.761 ± 0.032
0.403TrpHis: 0.403 ± 0.021
0.652TrpIle: 0.652 ± 0.03
0.436TrpLys: 0.436 ± 0.023
2.212TrpLeu: 2.212 ± 0.061
0.344TrpMet: 0.344 ± 0.018
0.421TrpAsn: 0.421 ± 0.02
0.6TrpPro: 0.6 ± 0.027
1.149TrpGln: 1.149 ± 0.043
0.963TrpArg: 0.963 ± 0.034
0.728TrpSer: 0.728 ± 0.031
0.535TrpThr: 0.535 ± 0.025
0.891TrpVal: 0.891 ± 0.033
0.229TrpTrp: 0.229 ± 0.017
0.355TrpTyr: 0.355 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.535TyrAla: 2.535 ± 0.054
0.339TyrCys: 0.339 ± 0.021
1.436TyrAsp: 1.436 ± 0.043
1.271TyrGlu: 1.271 ± 0.036
1.108TyrPhe: 1.108 ± 0.036
1.842TyrGly: 1.842 ± 0.047
0.556TyrHis: 0.556 ± 0.023
1.408TyrIle: 1.408 ± 0.037
1.019TyrLys: 1.019 ± 0.035
3.113TyrLeu: 3.113 ± 0.061
0.553TyrMet: 0.553 ± 0.024
0.934TyrAsn: 0.934 ± 0.034
1.293TyrPro: 1.293 ± 0.04
1.627TyrGln: 1.627 ± 0.043
1.738TyrArg: 1.738 ± 0.05
1.776TyrSer: 1.776 ± 0.043
1.341TyrThr: 1.341 ± 0.039
1.64TyrVal: 1.64 ± 0.048
0.416TyrTrp: 0.416 ± 0.023
0.775TyrTyr: 0.775 ± 0.031
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2792 proteins (900516 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski