Onion yellows phytoplasma (strain OY-M)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Candidatus Phytoplasma; Candidatus Phytoplasma asteris; Onion yellows phytoplasma

Average proteome isoelectric point is 7.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 730 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6YQI8|Q6YQI8_ONYPE HTH_21 domain-containing protein OS=Onion yellows phytoplasma (strain OY-M) OX=262768 GN=PAM_385 PE=4 SV=1
MM1 pKa = 7.4INKK4 pKa = 6.35TTIYY8 pKa = 10.42YY9 pKa = 10.31RR10 pKa = 11.84ADD12 pKa = 3.07KK13 pKa = 10.16TIDD16 pKa = 3.41YY17 pKa = 10.47IFEE20 pKa = 4.24NDD22 pKa = 3.72KK23 pKa = 9.46EE24 pKa = 4.25TGYY27 pKa = 10.34IIKK30 pKa = 8.71KK31 pKa = 6.82TQYY34 pKa = 10.49QSDD37 pKa = 4.13GTNIDD42 pKa = 3.66YY43 pKa = 10.97VSEE46 pKa = 4.08YY47 pKa = 11.2DD48 pKa = 3.47KK49 pKa = 11.23DD50 pKa = 3.37TGQEE54 pKa = 4.04VKK56 pKa = 8.92VTFYY60 pKa = 10.92QFDD63 pKa = 3.74GKK65 pKa = 9.25TIEE68 pKa = 5.69CIAEE72 pKa = 3.96YY73 pKa = 10.92DD74 pKa = 3.47KK75 pKa = 11.13GTCKK79 pKa = 10.11LVKK82 pKa = 9.72DD83 pKa = 3.69TTYY86 pKa = 11.08QSDD89 pKa = 3.28GKK91 pKa = 8.78TIRR94 pKa = 11.84KK95 pKa = 8.65INEE98 pKa = 3.82YY99 pKa = 9.61NQSTGEE105 pKa = 4.21SIKK108 pKa = 10.64DD109 pKa = 2.86IYY111 pKa = 9.89YY112 pKa = 10.57QIDD115 pKa = 3.91GKK117 pKa = 9.42TIAYY121 pKa = 9.36VNEE124 pKa = 3.91YY125 pKa = 10.36DD126 pKa = 5.37DD127 pKa = 5.32NGTLIQTIYY136 pKa = 10.86YY137 pKa = 10.0NDD139 pKa = 4.48DD140 pKa = 3.44GTVKK144 pKa = 10.74DD145 pKa = 4.54IINFF149 pKa = 3.62

Molecular weight: 17.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6YQ94|Q6YQ94_ONYPE Uncharacterized protein OS=Onion yellows phytoplasma (strain OY-M) OX=262768 GN=PAM_481 PE=4 SV=1
MM1 pKa = 7.28SHH3 pKa = 6.62GPSIRR8 pKa = 11.84YY9 pKa = 9.04RR10 pKa = 11.84KK11 pKa = 7.8ITKK14 pKa = 9.94SDD16 pKa = 3.57FRR18 pKa = 11.84PCSTCKK24 pKa = 10.39SCSQAPLCLYY34 pKa = 9.88ALQMISNHH42 pKa = 5.76FEE44 pKa = 3.88GTFEE48 pKa = 4.11RR49 pKa = 11.84LRR51 pKa = 11.84YY52 pKa = 9.78SLGGDD57 pKa = 3.29RR58 pKa = 11.84PSQTAHH64 pKa = 4.6QTLSLTLHH72 pKa = 5.84MCRR75 pKa = 11.84LEE77 pKa = 4.14TKK79 pKa = 9.51NNKK82 pKa = 9.71GGIPRR87 pKa = 11.84TTPEE91 pKa = 3.47KK92 pKa = 10.63LAFFSHH98 pKa = 6.57SFPPILYY105 pKa = 8.79ILFPISILSCSKK117 pKa = 10.87APWGLFVLSRR127 pKa = 11.84VTGIFTGNLISPRR140 pKa = 11.84LLLRR144 pKa = 11.84QRR146 pKa = 11.84PNRR149 pKa = 11.84YY150 pKa = 9.4AFRR153 pKa = 11.84AGRR156 pKa = 11.84NLPDD160 pKa = 3.22KK161 pKa = 10.12EE162 pKa = 3.91FRR164 pKa = 11.84YY165 pKa = 10.28LRR167 pKa = 11.84TVIVTAAVYY176 pKa = 10.0WDD178 pKa = 4.11FNSMLRR184 pKa = 11.84CLTSPFNLPAPGRR197 pKa = 11.84HH198 pKa = 4.28QPLYY202 pKa = 7.94ITLRR206 pKa = 11.84FSRR209 pKa = 11.84DD210 pKa = 2.82LCFF213 pKa = 4.91

Molecular weight: 24.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

730

0

730

204107

33

1571

279.6

32.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.51 ± 0.097

1.036 ± 0.031

4.597 ± 0.066

6.014 ± 0.1

5.69 ± 0.107

4.242 ± 0.081

2.056 ± 0.042

8.882 ± 0.097

10.788 ± 0.119

10.839 ± 0.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.858 ± 0.035

7.078 ± 0.092

3.263 ± 0.053

5.866 ± 0.105

2.642 ± 0.053

5.441 ± 0.066

5.884 ± 0.065

4.678 ± 0.096

0.64 ± 0.022

3.994 ± 0.076
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski