Amino acid dipepetide frequency for Mycoplasma iowae DK-CPA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.114AlaAla: 2.114 ± 0.099
0.452AlaCys: 0.452 ± 0.041
1.902AlaAsp: 1.902 ± 0.09
1.896AlaGlu: 1.896 ± 0.1
2.123AlaPhe: 2.123 ± 0.084
2.228AlaGly: 2.228 ± 0.128
0.504AlaHis: 0.504 ± 0.039
4.693AlaIle: 4.693 ± 0.137
4.096AlaLys: 4.096 ± 0.138
3.89AlaLeu: 3.89 ± 0.128
0.934AlaMet: 0.934 ± 0.055
3.35AlaAsn: 3.35 ± 0.107
0.968AlaPro: 0.968 ± 0.056
0.94AlaGln: 0.94 ± 0.052
1.069AlaArg: 1.069 ± 0.064
3.196AlaSer: 3.196 ± 0.119
2.624AlaThr: 2.624 ± 0.094
2.314AlaVal: 2.314 ± 0.101
0.396AlaTrp: 0.396 ± 0.034
1.712AlaTyr: 1.712 ± 0.066
0.0AlaXaa: 0.0 ± 0.0
Cys
0.356CysAla: 0.356 ± 0.031
0.129CysCys: 0.129 ± 0.022
0.406CysAsp: 0.406 ± 0.04
0.47CysGlu: 0.47 ± 0.043
0.553CysPhe: 0.553 ± 0.045
0.492CysGly: 0.492 ± 0.05
0.172CysHis: 0.172 ± 0.023
0.685CysIle: 0.685 ± 0.054
0.67CysLys: 0.67 ± 0.05
0.885CysLeu: 0.885 ± 0.058
0.141CysMet: 0.141 ± 0.024
0.458CysAsn: 0.458 ± 0.039
0.212CysPro: 0.212 ± 0.029
0.2CysGln: 0.2 ± 0.027
0.169CysArg: 0.169 ± 0.023
0.572CysSer: 0.572 ± 0.045
0.298CysThr: 0.298 ± 0.028
0.446CysVal: 0.446 ± 0.042
0.086CysTrp: 0.086 ± 0.018
0.344CysTyr: 0.344 ± 0.034
0.0CysXaa: 0.0 ± 0.0
Asp
2.409AspAla: 2.409 ± 0.084
0.393AspCys: 0.393 ± 0.041
3.466AspAsp: 3.466 ± 0.151
4.232AspGlu: 4.232 ± 0.154
3.503AspPhe: 3.503 ± 0.134
2.796AspGly: 2.796 ± 0.1
0.547AspHis: 0.547 ± 0.041
5.71AspIle: 5.71 ± 0.156
6.069AspLys: 6.069 ± 0.145
5.359AspLeu: 5.359 ± 0.137
0.885AspMet: 0.885 ± 0.055
4.788AspAsn: 4.788 ± 0.173
1.38AspPro: 1.38 ± 0.078
1.832AspGln: 1.832 ± 0.086
1.281AspArg: 1.281 ± 0.066
3.918AspSer: 3.918 ± 0.122
2.462AspThr: 2.462 ± 0.086
3.835AspVal: 3.835 ± 0.118
0.67AspTrp: 0.67 ± 0.048
3.076AspTyr: 3.076 ± 0.1
0.0AspXaa: 0.0 ± 0.0
Glu
2.311GluAla: 2.311 ± 0.109
0.366GluCys: 0.366 ± 0.04
2.922GluAsp: 2.922 ± 0.124
4.136GluGlu: 4.136 ± 0.239
3.187GluPhe: 3.187 ± 0.116
1.927GluGly: 1.927 ± 0.098
0.695GluHis: 0.695 ± 0.046
7.12GluIle: 7.12 ± 0.188
7.025GluLys: 7.025 ± 0.199
5.848GluLeu: 5.848 ± 0.176
1.398GluMet: 1.398 ± 0.077
6.22GluAsn: 6.22 ± 0.181
1.192GluPro: 1.192 ± 0.057
1.964GluGln: 1.964 ± 0.079
1.549GluArg: 1.549 ± 0.082
3.725GluSer: 3.725 ± 0.125
3.113GluThr: 3.113 ± 0.102
3.079GluVal: 3.079 ± 0.109
0.605GluTrp: 0.605 ± 0.048
2.757GluTyr: 2.757 ± 0.119
0.0GluXaa: 0.0 ± 0.0
Phe
2.486PheAla: 2.486 ± 0.094
0.473PheCys: 0.473 ± 0.042
3.623PheAsp: 3.623 ± 0.122
3.267PheGlu: 3.267 ± 0.129
3.064PhePhe: 3.064 ± 0.114
3.067PheGly: 3.067 ± 0.107
0.559PheHis: 0.559 ± 0.048
5.409PheIle: 5.409 ± 0.159
5.492PheLys: 5.492 ± 0.145
4.923PheLeu: 4.923 ± 0.158
0.943PheMet: 0.943 ± 0.054
4.588PheAsn: 4.588 ± 0.136
1.091PhePro: 1.091 ± 0.064
1.143PheGln: 1.143 ± 0.072
1.131PheArg: 1.131 ± 0.066
4.225PheSer: 4.225 ± 0.134
2.723PheThr: 2.723 ± 0.102
3.694PheVal: 3.694 ± 0.11
0.406PheTrp: 0.406 ± 0.034
2.591PheTyr: 2.591 ± 0.095
0.0PheXaa: 0.0 ± 0.0
Gly
2.311GlyAla: 2.311 ± 0.098
0.399GlyCys: 0.399 ± 0.034
2.437GlyAsp: 2.437 ± 0.101
2.274GlyGlu: 2.274 ± 0.089
3.236GlyPhe: 3.236 ± 0.103
3.009GlyGly: 3.009 ± 0.18
0.731GlyHis: 0.731 ± 0.053
4.803GlyIle: 4.803 ± 0.146
3.967GlyLys: 3.967 ± 0.134
4.299GlyLeu: 4.299 ± 0.132
0.993GlyMet: 0.993 ± 0.054
3.19GlyAsn: 3.19 ± 0.117
1.026GlyPro: 1.026 ± 0.085
1.414GlyGln: 1.414 ± 0.071
1.146GlyArg: 1.146 ± 0.072
3.663GlySer: 3.663 ± 0.149
3.082GlyThr: 3.082 ± 0.123
3.141GlyVal: 3.141 ± 0.101
0.443GlyTrp: 0.443 ± 0.038
2.329GlyTyr: 2.329 ± 0.088
0.0GlyXaa: 0.0 ± 0.0
His
0.525HisAla: 0.525 ± 0.041
0.129HisCys: 0.129 ± 0.019
0.648HisAsp: 0.648 ± 0.043
0.664HisGlu: 0.664 ± 0.049
0.679HisPhe: 0.679 ± 0.045
0.651HisGly: 0.651 ± 0.049
0.187HisHis: 0.187 ± 0.025
1.119HisIle: 1.119 ± 0.056
0.931HisLys: 0.931 ± 0.062
1.072HisLeu: 1.072 ± 0.06
0.24HisMet: 0.24 ± 0.029
1.008HisAsn: 1.008 ± 0.059
0.43HisPro: 0.43 ± 0.039
0.353HisGln: 0.353 ± 0.036
0.35HisArg: 0.35 ± 0.032
0.768HisSer: 0.768 ± 0.05
0.522HisThr: 0.522 ± 0.044
0.639HisVal: 0.639 ± 0.047
0.092HisTrp: 0.092 ± 0.014
0.482HisTyr: 0.482 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
4.567IleAla: 4.567 ± 0.146
0.946IleCys: 0.946 ± 0.062
6.93IleAsp: 6.93 ± 0.169
6.373IleGlu: 6.373 ± 0.213
5.0IlePhe: 5.0 ± 0.14
5.083IleGly: 5.083 ± 0.169
1.029IleHis: 1.029 ± 0.061
8.955IleIle: 8.955 ± 0.22
10.667IleLys: 10.667 ± 0.216
7.802IleLeu: 7.802 ± 0.216
1.733IleMet: 1.733 ± 0.072
9.228IleAsn: 9.228 ± 0.174
2.941IlePro: 2.941 ± 0.101
2.422IleGln: 2.422 ± 0.081
2.179IleArg: 2.179 ± 0.094
7.778IleSer: 7.778 ± 0.196
4.941IleThr: 4.941 ± 0.149
6.189IleVal: 6.189 ± 0.161
0.71IleTrp: 0.71 ± 0.053
4.09IleTyr: 4.09 ± 0.121
0.0IleXaa: 0.0 ± 0.0
Lys
3.66LysAla: 3.66 ± 0.125
0.446LysCys: 0.446 ± 0.039
6.232LysAsp: 6.232 ± 0.163
7.815LysGlu: 7.815 ± 0.212
4.588LysPhe: 4.588 ± 0.109
3.116LysGly: 3.116 ± 0.102
1.149LysHis: 1.149 ± 0.057
11.011LysIle: 11.011 ± 0.229
11.364LysLys: 11.364 ± 0.243
8.291LysLeu: 8.291 ± 0.183
2.422LysMet: 2.422 ± 0.085
10.562LysAsn: 10.562 ± 0.21
2.532LysPro: 2.532 ± 0.119
3.414LysGln: 3.414 ± 0.102
2.508LysArg: 2.508 ± 0.091
6.291LysSer: 6.291 ± 0.146
6.334LysThr: 6.334 ± 0.146
5.024LysVal: 5.024 ± 0.121
1.066LysTrp: 1.066 ± 0.06
4.868LysTyr: 4.868 ± 0.137
0.0LysXaa: 0.0 ± 0.0
Leu
3.685LeuAla: 3.685 ± 0.12
0.771LeuCys: 0.771 ± 0.051
5.187LeuAsp: 5.187 ± 0.135
5.141LeuGlu: 5.141 ± 0.179
4.898LeuPhe: 4.898 ± 0.16
4.016LeuGly: 4.016 ± 0.138
0.971LeuHis: 0.971 ± 0.059
9.047LeuIle: 9.047 ± 0.184
9.222LeuLys: 9.222 ± 0.183
7.938LeuLeu: 7.938 ± 0.172
1.595LeuMet: 1.595 ± 0.066
8.005LeuAsn: 8.005 ± 0.168
2.48LeuPro: 2.48 ± 0.102
1.899LeuGln: 1.899 ± 0.074
1.841LeuArg: 1.841 ± 0.091
7.363LeuSer: 7.363 ± 0.175
5.049LeuThr: 5.049 ± 0.127
5.04LeuVal: 5.04 ± 0.12
0.762LeuTrp: 0.762 ± 0.053
3.353LeuTyr: 3.353 ± 0.111
0.0LeuXaa: 0.0 ± 0.0
Met
0.922MetAla: 0.922 ± 0.058
0.154MetCys: 0.154 ± 0.022
0.946MetAsp: 0.946 ± 0.058
1.033MetGlu: 1.033 ± 0.066
1.134MetPhe: 1.134 ± 0.065
1.033MetGly: 1.033 ± 0.061
0.243MetHis: 0.243 ± 0.03
1.736MetIle: 1.736 ± 0.073
1.951MetLys: 1.951 ± 0.07
1.626MetLeu: 1.626 ± 0.071
0.467MetMet: 0.467 ± 0.045
1.567MetAsn: 1.567 ± 0.067
0.593MetPro: 0.593 ± 0.046
0.455MetGln: 0.455 ± 0.041
0.421MetArg: 0.421 ± 0.039
1.404MetSer: 1.404 ± 0.062
0.913MetThr: 0.913 ± 0.051
1.128MetVal: 1.128 ± 0.076
0.151MetTrp: 0.151 ± 0.023
0.661MetTyr: 0.661 ± 0.04
0.0MetXaa: 0.0 ± 0.0
Asn
3.187AsnAla: 3.187 ± 0.109
0.51AsnCys: 0.51 ± 0.043
5.636AsnAsp: 5.636 ± 0.183
5.565AsnGlu: 5.565 ± 0.165
4.852AsnPhe: 4.852 ± 0.132
4.563AsnGly: 4.563 ± 0.177
1.029AsnHis: 1.029 ± 0.067
8.236AsnIle: 8.236 ± 0.203
10.623AsnLys: 10.623 ± 0.211
7.286AsnLeu: 7.286 ± 0.164
1.294AsnMet: 1.294 ± 0.065
9.379AsnAsn: 9.379 ± 0.283
2.397AsnPro: 2.397 ± 0.092
3.153AsnGln: 3.153 ± 0.117
1.948AsnArg: 1.948 ± 0.09
6.432AsnSer: 6.432 ± 0.17
3.967AsnThr: 3.967 ± 0.124
4.871AsnVal: 4.871 ± 0.126
0.956AsnTrp: 0.956 ± 0.054
4.081AsnTyr: 4.081 ± 0.122
0.0AsnXaa: 0.0 ± 0.0
Pro
0.891ProAla: 0.891 ± 0.058
0.166ProCys: 0.166 ± 0.022
1.143ProAsp: 1.143 ± 0.065
1.69ProGlu: 1.69 ± 0.078
1.506ProPhe: 1.506 ± 0.076
1.202ProGly: 1.202 ± 0.067
0.384ProHis: 0.384 ± 0.034
2.904ProIle: 2.904 ± 0.1
2.222ProLys: 2.222 ± 0.081
2.237ProLeu: 2.237 ± 0.094
0.439ProMet: 0.439 ± 0.045
2.428ProAsn: 2.428 ± 0.096
0.565ProPro: 0.565 ± 0.054
0.771ProGln: 0.771 ± 0.054
0.529ProArg: 0.529 ± 0.049
2.17ProSer: 2.17 ± 0.091
1.785ProThr: 1.785 ± 0.082
1.552ProVal: 1.552 ± 0.08
0.252ProTrp: 0.252 ± 0.028
1.162ProTyr: 1.162 ± 0.062
0.0ProXaa: 0.0 ± 0.0
Gln
1.165GlnAla: 1.165 ± 0.06
0.132GlnCys: 0.132 ± 0.022
1.407GlnAsp: 1.407 ± 0.068
1.982GlnGlu: 1.982 ± 0.095
1.245GlnPhe: 1.245 ± 0.06
1.011GlnGly: 1.011 ± 0.058
0.304GlnHis: 0.304 ± 0.027
2.962GlnIle: 2.962 ± 0.094
3.088GlnLys: 3.088 ± 0.103
2.557GlnLeu: 2.557 ± 0.099
0.645GlnMet: 0.645 ± 0.052
2.769GlnAsn: 2.769 ± 0.106
0.679GlnPro: 0.679 ± 0.056
1.14GlnGln: 1.14 ± 0.066
0.839GlnArg: 0.839 ± 0.05
1.859GlnSer: 1.859 ± 0.08
1.761GlnThr: 1.761 ± 0.073
1.401GlnVal: 1.401 ± 0.066
0.304GlnTrp: 0.304 ± 0.033
1.269GlnTyr: 1.269 ± 0.069
0.0GlnXaa: 0.0 ± 0.0
Arg
0.971ArgAla: 0.971 ± 0.052
0.194ArgCys: 0.194 ± 0.026
1.257ArgAsp: 1.257 ± 0.067
1.383ArgGlu: 1.383 ± 0.063
1.159ArgPhe: 1.159 ± 0.073
1.088ArgGly: 1.088 ± 0.068
0.332ArgHis: 0.332 ± 0.037
2.292ArgIle: 2.292 ± 0.088
2.594ArgLys: 2.594 ± 0.095
2.028ArgLeu: 2.028 ± 0.089
0.618ArgMet: 0.618 ± 0.048
1.961ArgAsn: 1.961 ± 0.078
0.667ArgPro: 0.667 ± 0.06
0.731ArgGln: 0.731 ± 0.045
0.796ArgArg: 0.796 ± 0.05
1.447ArgSer: 1.447 ± 0.063
1.214ArgThr: 1.214 ± 0.063
1.435ArgVal: 1.435 ± 0.089
0.261ArgTrp: 0.261 ± 0.029
1.091ArgTyr: 1.091 ± 0.062
0.0ArgXaa: 0.0 ± 0.0
Ser
2.704SerAla: 2.704 ± 0.106
0.67SerCys: 0.67 ± 0.042
4.118SerAsp: 4.118 ± 0.139
4.038SerGlu: 4.038 ± 0.126
4.693SerPhe: 4.693 ± 0.144
3.875SerGly: 3.875 ± 0.147
0.731SerHis: 0.731 ± 0.052
7.341SerIle: 7.341 ± 0.187
7.354SerLys: 7.354 ± 0.154
7.136SerLeu: 7.136 ± 0.174
1.177SerMet: 1.177 ± 0.067
6.46SerAsn: 6.46 ± 0.2
1.681SerPro: 1.681 ± 0.069
2.228SerGln: 2.228 ± 0.095
1.813SerArg: 1.813 ± 0.077
6.189SerSer: 6.189 ± 0.213
4.007SerThr: 4.007 ± 0.134
3.811SerVal: 3.811 ± 0.133
0.787SerTrp: 0.787 ± 0.058
3.178SerTyr: 3.178 ± 0.111
0.0SerXaa: 0.0 ± 0.0
Thr
2.17ThrAla: 2.17 ± 0.086
0.295ThrCys: 0.295 ± 0.031
2.778ThrAsp: 2.778 ± 0.095
2.508ThrGlu: 2.508 ± 0.092
3.009ThrPhe: 3.009 ± 0.107
2.867ThrGly: 2.867 ± 0.123
0.679ThrHis: 0.679 ± 0.048
5.777ThrIle: 5.777 ± 0.134
5.141ThrLys: 5.141 ± 0.124
4.739ThrLeu: 4.739 ± 0.13
0.756ThrMet: 0.756 ± 0.044
5.074ThrAsn: 5.074 ± 0.161
1.939ThrPro: 1.939 ± 0.083
1.438ThrGln: 1.438 ± 0.071
1.266ThrArg: 1.266 ± 0.069
4.536ThrSer: 4.536 ± 0.151
3.681ThrThr: 3.681 ± 0.158
2.793ThrVal: 2.793 ± 0.094
0.403ThrTrp: 0.403 ± 0.038
2.434ThrTyr: 2.434 ± 0.087
0.0ThrXaa: 0.0 ± 0.0
Val
2.833ValAla: 2.833 ± 0.111
0.612ValCys: 0.612 ± 0.048
3.869ValAsp: 3.869 ± 0.113
3.393ValGlu: 3.393 ± 0.139
2.956ValPhe: 2.956 ± 0.099
3.055ValGly: 3.055 ± 0.13
0.658ValHis: 0.658 ± 0.052
5.169ValIle: 5.169 ± 0.131
4.852ValLys: 4.852 ± 0.127
5.289ValLeu: 5.289 ± 0.14
0.974ValMet: 0.974 ± 0.052
4.576ValAsn: 4.576 ± 0.116
1.862ValPro: 1.862 ± 0.079
1.447ValGln: 1.447 ± 0.072
1.272ValArg: 1.272 ± 0.07
4.45ValSer: 4.45 ± 0.129
3.119ValThr: 3.119 ± 0.11
3.927ValVal: 3.927 ± 0.127
0.492ValTrp: 0.492 ± 0.045
2.597ValTyr: 2.597 ± 0.088
0.0ValXaa: 0.0 ± 0.0
Trp
0.387TrpAla: 0.387 ± 0.038
0.077TrpCys: 0.077 ± 0.015
0.596TrpAsp: 0.596 ± 0.049
0.464TrpGlu: 0.464 ± 0.042
0.645TrpPhe: 0.645 ± 0.052
0.461TrpGly: 0.461 ± 0.033
0.095TrpHis: 0.095 ± 0.02
1.005TrpIle: 1.005 ± 0.069
0.864TrpLys: 0.864 ± 0.05
0.805TrpLeu: 0.805 ± 0.051
0.175TrpMet: 0.175 ± 0.023
0.839TrpAsn: 0.839 ± 0.059
0.175TrpPro: 0.175 ± 0.021
0.206TrpGln: 0.206 ± 0.023
0.249TrpArg: 0.249 ± 0.03
0.565TrpSer: 0.565 ± 0.043
0.51TrpThr: 0.51 ± 0.04
0.581TrpVal: 0.581 ± 0.049
0.123TrpTrp: 0.123 ± 0.022
0.565TrpTyr: 0.565 ± 0.049
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.749TyrAla: 1.749 ± 0.075
0.43TyrCys: 0.43 ± 0.044
3.107TyrAsp: 3.107 ± 0.101
2.815TyrGlu: 2.815 ± 0.101
2.867TyrPhe: 2.867 ± 0.108
2.354TyrGly: 2.354 ± 0.093
0.492TyrHis: 0.492 ± 0.038
3.589TyrIle: 3.589 ± 0.098
4.499TyrLys: 4.499 ± 0.118
4.256TyrLeu: 4.256 ± 0.128
0.704TyrMet: 0.704 ± 0.039
3.519TyrAsn: 3.519 ± 0.117
1.186TyrPro: 1.186 ± 0.065
1.371TyrGln: 1.371 ± 0.064
1.162TyrArg: 1.162 ± 0.06
3.439TyrSer: 3.439 ± 0.125
2.176TyrThr: 2.176 ± 0.087
2.501TyrVal: 2.501 ± 0.078
0.424TyrTrp: 0.424 ± 0.037
2.062TyrTyr: 2.062 ± 0.1
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 854 proteins (325412 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski