Amino acid dipepetide frequency for Methylobacterium sp. YIM 132548

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.14AlaAla: 22.14 ± 0.218	1.328AlaCys: 1.328 ± 0.032	7.155AlaAsp: 7.155 ± 0.079	8.889AlaGlu: 8.889 ± 0.083	4.807AlaPhe: 4.807 ± 0.06	13.369AlaGly: 13.369 ± 0.123	2.464AlaHis: 2.464 ± 0.042	6.111AlaIle: 6.111 ± 0.063	3.307AlaLys: 3.307 ± 0.064	15.957AlaLeu: 15.957 ± 0.133	3.358AlaMet: 3.358 ± 0.047	2.539AlaAsn: 2.539 ± 0.049	7.686AlaPro: 7.686 ± 0.098	4.264AlaGln: 4.264 ± 0.056	12.009AlaArg: 12.009 ± 0.109	6.852AlaSer: 6.852 ± 0.083	6.672AlaThr: 6.672 ± 0.077	10.078AlaVal: 10.078 ± 0.089	1.733AlaTrp: 1.733 ± 0.036	2.83AlaTyr: 2.83 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	1.038CysAla: 1.038 ± 0.03	0.085CysCys: 0.085 ± 0.008	0.471CysAsp: 0.471 ± 0.016	0.431CysGlu: 0.431 ± 0.017	0.266CysPhe: 0.266 ± 0.014	0.924CysGly: 0.924 ± 0.029	0.201CysHis: 0.201 ± 0.013	0.354CysIle: 0.354 ± 0.015	0.126CysLys: 0.126 ± 0.009	0.968CysLeu: 0.968 ± 0.024	0.144CysMet: 0.144 ± 0.008	0.177CysAsn: 0.177 ± 0.011	0.453CysPro: 0.453 ± 0.02	0.193CysGln: 0.193 ± 0.011	0.737CysArg: 0.737 ± 0.026	0.388CysSer: 0.388 ± 0.015	0.415CysThr: 0.415 ± 0.018	0.589CysVal: 0.589 ± 0.019	0.1CysTrp: 0.1 ± 0.01	0.154CysTyr: 0.154 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.736AspAla: 7.736 ± 0.081	0.402AspCys: 0.402 ± 0.016	2.685AspAsp: 2.685 ± 0.045	2.963AspGlu: 2.963 ± 0.047	1.836AspPhe: 1.836 ± 0.032	5.351AspGly: 5.351 ± 0.07	1.145AspHis: 1.145 ± 0.027	2.18AspIle: 2.18 ± 0.039	1.172AspLys: 1.172 ± 0.03	6.608AspLeu: 6.608 ± 0.072	0.907AspMet: 0.907 ± 0.023	0.864AspAsn: 0.864 ± 0.025	4.228AspPro: 4.228 ± 0.057	1.416AspGln: 1.416 ± 0.032	4.724AspArg: 4.724 ± 0.059	1.722AspSer: 1.722 ± 0.036	2.443AspThr: 2.443 ± 0.05	3.936AspVal: 3.936 ± 0.057	0.864AspTrp: 0.864 ± 0.023	1.189AspTyr: 1.189 ± 0.028	0.0AspXaa: 0.0 ± 0.0
Glu	9.669GluAla: 9.669 ± 0.094	0.321GluCys: 0.321 ± 0.014	2.449GluAsp: 2.449 ± 0.041	2.61GluGlu: 2.61 ± 0.039	1.345GluPhe: 1.345 ± 0.033	4.613GluGly: 4.613 ± 0.056	1.123GluHis: 1.123 ± 0.028	3.207GluIle: 3.207 ± 0.046	1.43GluLys: 1.43 ± 0.036	4.147GluLeu: 4.147 ± 0.053	1.236GluMet: 1.236 ± 0.029	1.123GluAsn: 1.123 ± 0.027	3.299GluPro: 3.299 ± 0.055	1.542GluGln: 1.542 ± 0.034	5.732GluArg: 5.732 ± 0.067	2.197GluSer: 2.197 ± 0.039	3.623GluThr: 3.623 ± 0.053	3.642GluVal: 3.642 ± 0.053	0.546GluTrp: 0.546 ± 0.018	0.728GluTyr: 0.728 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	4.608PheAla: 4.608 ± 0.054	0.347PheCys: 0.347 ± 0.017	2.234PheAsp: 2.234 ± 0.043	1.924PheGlu: 1.924 ± 0.036	1.164PhePhe: 1.164 ± 0.032	3.613PheGly: 3.613 ± 0.054	0.664PheHis: 0.664 ± 0.022	1.215PheIle: 1.215 ± 0.028	0.725PheLys: 0.725 ± 0.025	3.269PheLeu: 3.269 ± 0.053	0.583PheMet: 0.583 ± 0.021	0.794PheAsn: 0.794 ± 0.026	1.542PhePro: 1.542 ± 0.032	0.858PheGln: 0.858 ± 0.023	2.435PheArg: 2.435 ± 0.041	1.856PheSer: 1.856 ± 0.036	1.873PheThr: 1.873 ± 0.037	2.753PheVal: 2.753 ± 0.04	0.478PheTrp: 0.478 ± 0.019	0.752PheTyr: 0.752 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	11.532GlyAla: 11.532 ± 0.093	0.845GlyCys: 0.845 ± 0.024	4.322GlyAsp: 4.322 ± 0.059	4.782GlyGlu: 4.782 ± 0.058	3.623GlyPhe: 3.623 ± 0.052	8.109GlyGly: 8.109 ± 0.113	1.97GlyHis: 1.97 ± 0.035	4.483GlyIle: 4.483 ± 0.055	2.39GlyLys: 2.39 ± 0.047	10.828GlyLeu: 10.828 ± 0.099	1.907GlyMet: 1.907 ± 0.032	1.869GlyAsn: 1.869 ± 0.042	4.832GlyPro: 4.832 ± 0.058	2.901GlyGln: 2.901 ± 0.042	8.034GlyArg: 8.034 ± 0.093	4.965GlySer: 4.965 ± 0.064	5.266GlyThr: 5.266 ± 0.07	6.054GlyVal: 6.054 ± 0.066	1.343GlyTrp: 1.343 ± 0.032	2.241GlyTyr: 2.241 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.705HisAla: 2.705 ± 0.046	0.216HisCys: 0.216 ± 0.011	1.178HisAsp: 1.178 ± 0.027	0.953HisGlu: 0.953 ± 0.025	0.702HisPhe: 0.702 ± 0.021	1.97HisGly: 1.97 ± 0.038	0.574HisHis: 0.574 ± 0.023	0.68HisIle: 0.68 ± 0.022	0.337HisLys: 0.337 ± 0.014	2.187HisLeu: 2.187 ± 0.041	0.373HisMet: 0.373 ± 0.017	0.364HisAsn: 0.364 ± 0.015	1.462HisPro: 1.462 ± 0.033	0.481HisGln: 0.481 ± 0.016	1.617HisArg: 1.617 ± 0.028	0.726HisSer: 0.726 ± 0.02	0.731HisThr: 0.731 ± 0.023	1.444HisVal: 1.444 ± 0.035	0.293HisTrp: 0.293 ± 0.014	0.491HisTyr: 0.491 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	6.842IleAla: 6.842 ± 0.074	0.422IleCys: 0.422 ± 0.017	3.01IleAsp: 3.01 ± 0.045	3.144IleGlu: 3.144 ± 0.044	1.234IlePhe: 1.234 ± 0.029	4.706IleGly: 4.706 ± 0.059	0.778IleHis: 0.778 ± 0.026	1.46IleIle: 1.46 ± 0.034	0.991IleLys: 0.991 ± 0.028	4.542IleLeu: 4.542 ± 0.063	0.658IleMet: 0.658 ± 0.021	0.996IleAsn: 0.996 ± 0.028	2.167IlePro: 2.167 ± 0.037	1.093IleGln: 1.093 ± 0.032	3.296IleArg: 3.296 ± 0.046	2.036IleSer: 2.036 ± 0.041	2.36IleThr: 2.36 ± 0.043	3.85IleVal: 3.85 ± 0.051	0.464IleTrp: 0.464 ± 0.022	0.856IleTyr: 0.856 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	3.555LysAla: 3.555 ± 0.065	0.109LysCys: 0.109 ± 0.008	1.314LysAsp: 1.314 ± 0.033	1.004LysGlu: 1.004 ± 0.028	0.584LysPhe: 0.584 ± 0.021	2.083LysGly: 2.083 ± 0.039	0.384LysHis: 0.384 ± 0.015	1.092LysIle: 1.092 ± 0.028	0.741LysLys: 0.741 ± 0.027	2.418LysLeu: 2.418 ± 0.046	0.461LysMet: 0.461 ± 0.016	0.538LysAsn: 0.538 ± 0.019	1.905LysPro: 1.905 ± 0.045	0.645LysGln: 0.645 ± 0.021	1.972LysArg: 1.972 ± 0.037	1.236LysSer: 1.236 ± 0.032	1.324LysThr: 1.324 ± 0.028	1.851LysVal: 1.851 ± 0.042	0.227LysTrp: 0.227 ± 0.012	0.406LysTyr: 0.406 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	16.518LeuAla: 16.518 ± 0.151	0.962LeuCys: 0.962 ± 0.026	6.404LeuAsp: 6.404 ± 0.071	4.716LeuGlu: 4.716 ± 0.064	3.397LeuPhe: 3.397 ± 0.053	9.307LeuGly: 9.307 ± 0.088	1.949LeuHis: 1.949 ± 0.034	4.608LeuIle: 4.608 ± 0.07	2.786LeuLys: 2.786 ± 0.047	9.462LeuLeu: 9.462 ± 0.089	2.017LeuMet: 2.017 ± 0.043	2.301LeuAsn: 2.301 ± 0.043	6.137LeuPro: 6.137 ± 0.078	2.517LeuGln: 2.517 ± 0.042	8.303LeuArg: 8.303 ± 0.085	6.091LeuSer: 6.091 ± 0.074	5.974LeuThr: 5.974 ± 0.068	8.229LeuVal: 8.229 ± 0.084	1.113LeuTrp: 1.113 ± 0.03	1.993LeuTyr: 1.993 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	2.544MetAla: 2.544 ± 0.045	0.111MetCys: 0.111 ± 0.008	0.851MetAsp: 0.851 ± 0.021	0.833MetGlu: 0.833 ± 0.027	0.479MetPhe: 0.479 ± 0.017	1.492MetGly: 1.492 ± 0.032	0.342MetHis: 0.342 ± 0.015	1.018MetIle: 1.018 ± 0.026	0.617MetLys: 0.617 ± 0.023	2.079MetLeu: 2.079 ± 0.038	0.496MetMet: 0.496 ± 0.018	0.56MetAsn: 0.56 ± 0.018	1.535MetPro: 1.535 ± 0.028	0.629MetGln: 0.629 ± 0.018	1.955MetArg: 1.955 ± 0.035	1.417MetSer: 1.417 ± 0.031	1.434MetThr: 1.434 ± 0.03	1.323MetVal: 1.323 ± 0.027	0.141MetTrp: 0.141 ± 0.01	0.218MetTyr: 0.218 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.924AsnAla: 2.924 ± 0.046	0.178AsnCys: 0.178 ± 0.011	1.109AsnAsp: 1.109 ± 0.03	0.975AsnGlu: 0.975 ± 0.024	0.697AsnPhe: 0.697 ± 0.024	2.017AsnGly: 2.017 ± 0.043	0.415AsnHis: 0.415 ± 0.017	0.885AsnIle: 0.885 ± 0.024	0.463AsnLys: 0.463 ± 0.019	2.355AsnLeu: 2.355 ± 0.041	0.402AsnMet: 0.402 ± 0.02	0.51AsnAsn: 0.51 ± 0.021	1.692AsnPro: 1.692 ± 0.038	0.568AsnGln: 0.568 ± 0.019	1.704AsnArg: 1.704 ± 0.033	0.853AsnSer: 0.853 ± 0.029	1.027AsnThr: 1.027 ± 0.029	1.61AsnVal: 1.61 ± 0.034	0.297AsnTrp: 0.297 ± 0.015	0.479AsnTyr: 0.479 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	8.5ProAla: 8.5 ± 0.11	0.371ProCys: 0.371 ± 0.015	4.205ProAsp: 4.205 ± 0.063	4.308ProGlu: 4.308 ± 0.061	1.977ProPhe: 1.977 ± 0.037	6.032ProGly: 6.032 ± 0.07	1.098ProHis: 1.098 ± 0.028	2.261ProIle: 2.261 ± 0.037	1.49ProLys: 1.49 ± 0.035	5.177ProLeu: 5.177 ± 0.055	1.159ProMet: 1.159 ± 0.031	1.312ProAsn: 1.312 ± 0.029	3.447ProPro: 3.447 ± 0.061	1.535ProGln: 1.535 ± 0.034	4.184ProArg: 4.184 ± 0.047	2.991ProSer: 2.991 ± 0.044	2.712ProThr: 2.712 ± 0.047	4.73ProVal: 4.73 ± 0.052	0.731ProTrp: 0.731 ± 0.024	1.123ProTyr: 1.123 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	4.449GlnAla: 4.449 ± 0.056	0.181GlnCys: 0.181 ± 0.01	1.38GlnAsp: 1.38 ± 0.03	1.23GlnGlu: 1.23 ± 0.026	0.844GlnPhe: 0.844 ± 0.025	2.535GlnGly: 2.535 ± 0.044	0.5GlnHis: 0.5 ± 0.016	1.55GlnIle: 1.55 ± 0.029	0.758GlnLys: 0.758 ± 0.025	2.207GlnLeu: 2.207 ± 0.04	0.568GlnMet: 0.568 ± 0.017	0.689GlnAsn: 0.689 ± 0.022	1.712GlnPro: 1.712 ± 0.038	0.887GlnGln: 0.887 ± 0.03	2.276GlnArg: 2.276 ± 0.039	1.485GlnSer: 1.485 ± 0.03	1.539GlnThr: 1.539 ± 0.029	2.142GlnVal: 2.142 ± 0.043	0.272GlnTrp: 0.272 ± 0.014	0.486GlnTyr: 0.486 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	11.041ArgAla: 11.041 ± 0.108	0.57ArgCys: 0.57 ± 0.019	4.699ArgAsp: 4.699 ± 0.052	4.584ArgGlu: 4.584 ± 0.061	3.117ArgPhe: 3.117 ± 0.049	6.192ArgGly: 6.192 ± 0.075	1.9ArgHis: 1.9 ± 0.038	4.368ArgIle: 4.368 ± 0.047	1.778ArgLys: 1.778 ± 0.039	9.445ArgLeu: 9.445 ± 0.09	1.807ArgMet: 1.807 ± 0.033	1.797ArgAsn: 1.797 ± 0.034	4.844ArgPro: 4.844 ± 0.062	2.446ArgGln: 2.446 ± 0.036	7.626ArgArg: 7.626 ± 0.097	4.145ArgSer: 4.145 ± 0.059	4.122ArgThr: 4.122 ± 0.055	5.641ArgVal: 5.641 ± 0.069	1.033ArgTrp: 1.033 ± 0.026	1.788ArgTyr: 1.788 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	6.465SerAla: 6.465 ± 0.076	0.376SerCys: 0.376 ± 0.016	2.615SerAsp: 2.615 ± 0.046	2.716SerGlu: 2.716 ± 0.044	1.953SerPhe: 1.953 ± 0.035	5.413SerGly: 5.413 ± 0.066	0.93SerHis: 0.93 ± 0.028	2.088SerIle: 2.088 ± 0.038	1.09SerLys: 1.09 ± 0.029	5.62SerLeu: 5.62 ± 0.07	0.928SerMet: 0.928 ± 0.023	1.023SerAsn: 1.023 ± 0.033	2.958SerPro: 2.958 ± 0.047	1.372SerGln: 1.372 ± 0.029	3.712SerArg: 3.712 ± 0.052	2.335SerSer: 2.335 ± 0.052	2.406SerThr: 2.406 ± 0.043	3.745SerVal: 3.745 ± 0.046	0.615SerTrp: 0.615 ± 0.021	1.129SerTyr: 1.129 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	6.651ThrAla: 6.651 ± 0.085	0.433ThrCys: 0.433 ± 0.017	2.674ThrAsp: 2.674 ± 0.038	2.513ThrGlu: 2.513 ± 0.04	1.911ThrPhe: 1.911 ± 0.037	5.605ThrGly: 5.605 ± 0.063	0.993ThrHis: 0.993 ± 0.025	2.674ThrIle: 2.674 ± 0.043	1.161ThrLys: 1.161 ± 0.025	6.12ThrLeu: 6.12 ± 0.06	0.978ThrMet: 0.978 ± 0.027	1.168ThrAsn: 1.168 ± 0.03	3.407ThrPro: 3.407 ± 0.051	1.378ThrGln: 1.378 ± 0.027	3.866ThrArg: 3.866 ± 0.052	2.467ThrSer: 2.467 ± 0.047	2.781ThrThr: 2.781 ± 0.051	4.365ThrVal: 4.365 ± 0.065	0.669ThrTrp: 0.669 ± 0.021	1.178ThrTyr: 1.178 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	10.588ValAla: 10.588 ± 0.094	0.613ValCys: 0.613 ± 0.021	3.776ValAsp: 3.776 ± 0.055	4.451ValGlu: 4.451 ± 0.061	2.503ValPhe: 2.503 ± 0.044	5.979ValGly: 5.979 ± 0.066	1.348ValHis: 1.348 ± 0.031	3.441ValIle: 3.441 ± 0.046	1.73ValLys: 1.73 ± 0.037	7.685ValLeu: 7.685 ± 0.073	1.535ValMet: 1.535 ± 0.035	1.676ValAsn: 1.676 ± 0.043	4.385ValPro: 4.385 ± 0.054	2.019ValGln: 2.019 ± 0.041	5.632ValArg: 5.632 ± 0.071	4.141ValSer: 4.141 ± 0.062	4.518ValThr: 4.518 ± 0.057	6.107ValVal: 6.107 ± 0.062	0.825ValTrp: 0.825 ± 0.022	1.318ValTyr: 1.318 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.235TrpAla: 1.235 ± 0.029	0.134TrpCys: 0.134 ± 0.01	0.558TrpAsp: 0.558 ± 0.022	0.464TrpGlu: 0.464 ± 0.017	0.49TrpPhe: 0.49 ± 0.02	0.845TrpGly: 0.845 ± 0.025	0.304TrpHis: 0.304 ± 0.014	0.589TrpIle: 0.589 ± 0.019	0.32TrpLys: 0.32 ± 0.014	1.539TrpLeu: 1.539 ± 0.033	0.278TrpMet: 0.278 ± 0.014	0.366TrpAsn: 0.366 ± 0.016	0.662TrpPro: 0.662 ± 0.023	0.415TrpGln: 0.415 ± 0.014	1.302TrpArg: 1.302 ± 0.027	0.785TrpSer: 0.785 ± 0.023	0.794TrpThr: 0.794 ± 0.024	0.674TrpVal: 0.674 ± 0.023	0.204TrpTrp: 0.204 ± 0.012	0.264TrpTyr: 0.264 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.805TyrAla: 2.805 ± 0.043	0.209TyrCys: 0.209 ± 0.011	1.285TyrAsp: 1.285 ± 0.03	1.093TyrGlu: 1.093 ± 0.025	0.758TyrPhe: 0.758 ± 0.026	2.14TyrGly: 2.14 ± 0.047	0.404TyrHis: 0.404 ± 0.014	0.643TyrIle: 0.643 ± 0.021	0.438TyrLys: 0.438 ± 0.015	2.165TyrLeu: 2.165 ± 0.039	0.28TyrMet: 0.28 ± 0.012	0.494TyrAsn: 0.494 ± 0.022	0.991TyrPro: 0.991 ± 0.024	0.531TyrGln: 0.531 ± 0.019	1.827TyrArg: 1.827 ± 0.036	0.801TyrSer: 0.801 ± 0.024	0.98TyrThr: 0.98 ± 0.029	1.506TyrVal: 1.506 ± 0.032	0.273TyrTrp: 0.273 ± 0.012	0.471TyrTyr: 0.471 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5304 proteins (1615088 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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