Amino acid dipepetide frequency for Synechococcus phage S-SM2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.804AlaAla: 4.804 ± 0.354
0.619AlaCys: 0.619 ± 0.12
4.071AlaAsp: 4.071 ± 0.285
3.615AlaGlu: 3.615 ± 0.32
1.905AlaPhe: 1.905 ± 0.191
5.862AlaGly: 5.862 ± 0.527
0.814AlaHis: 0.814 ± 0.116
3.615AlaIle: 3.615 ± 0.232
4.185AlaLys: 4.185 ± 0.418
4.706AlaLeu: 4.706 ± 0.35
1.579AlaMet: 1.579 ± 0.173
3.452AlaAsn: 3.452 ± 0.251
2.898AlaPro: 2.898 ± 0.215
2.019AlaGln: 2.019 ± 0.204
2.329AlaArg: 2.329 ± 0.161
4.217AlaSer: 4.217 ± 0.26
4.999AlaThr: 4.999 ± 0.402
4.234AlaVal: 4.234 ± 0.292
0.554AlaTrp: 0.554 ± 0.111
2.003AlaTyr: 2.003 ± 0.17
0.0AlaXaa: 0.0 ± 0.0
Cys
0.733CysAla: 0.733 ± 0.132
0.065CysCys: 0.065 ± 0.032
0.716CysAsp: 0.716 ± 0.121
0.586CysGlu: 0.586 ± 0.104
0.326CysPhe: 0.326 ± 0.076
0.733CysGly: 0.733 ± 0.148
0.244CysHis: 0.244 ± 0.058
0.619CysIle: 0.619 ± 0.118
0.749CysLys: 0.749 ± 0.122
0.602CysLeu: 0.602 ± 0.104
0.309CysMet: 0.309 ± 0.083
0.423CysAsn: 0.423 ± 0.09
0.554CysPro: 0.554 ± 0.102
0.391CysGln: 0.391 ± 0.076
0.505CysArg: 0.505 ± 0.094
0.554CysSer: 0.554 ± 0.086
0.554CysThr: 0.554 ± 0.095
0.554CysVal: 0.554 ± 0.103
0.147CysTrp: 0.147 ± 0.05
0.407CysTyr: 0.407 ± 0.078
0.0CysXaa: 0.0 ± 0.0
Asp
4.234AspAla: 4.234 ± 0.23
0.749AspCys: 0.749 ± 0.119
4.999AspAsp: 4.999 ± 0.348
4.462AspGlu: 4.462 ± 0.291
3.436AspPhe: 3.436 ± 0.252
5.162AspGly: 5.162 ± 0.417
1.238AspHis: 1.238 ± 0.126
4.299AspIle: 4.299 ± 0.264
4.152AspLys: 4.152 ± 0.335
4.999AspLeu: 4.999 ± 0.298
1.482AspMet: 1.482 ± 0.176
3.273AspAsn: 3.273 ± 0.278
2.947AspPro: 2.947 ± 0.192
1.824AspGln: 1.824 ± 0.153
2.329AspArg: 2.329 ± 0.208
4.136AspSer: 4.136 ± 0.269
3.843AspThr: 3.843 ± 0.284
4.592AspVal: 4.592 ± 0.33
1.14AspTrp: 1.14 ± 0.174
3.224AspTyr: 3.224 ± 0.271
0.0AspXaa: 0.0 ± 0.0
Glu
3.224GluAla: 3.224 ± 0.19
0.798GluCys: 0.798 ± 0.153
4.266GluAsp: 4.266 ± 0.42
5.797GluGlu: 5.797 ± 0.505
3.192GluPhe: 3.192 ± 0.229
4.413GluGly: 4.413 ± 0.333
1.172GluHis: 1.172 ± 0.132
4.625GluIle: 4.625 ± 0.32
4.869GluLys: 4.869 ± 0.418
5.357GluLeu: 5.357 ± 0.331
1.661GluMet: 1.661 ± 0.228
3.875GluAsn: 3.875 ± 0.279
1.954GluPro: 1.954 ± 0.185
2.589GluGln: 2.589 ± 0.296
2.866GluArg: 2.866 ± 0.209
4.152GluSer: 4.152 ± 0.285
3.989GluThr: 3.989 ± 0.274
4.331GluVal: 4.331 ± 0.292
0.944GluTrp: 0.944 ± 0.165
2.85GluTyr: 2.85 ± 0.238
0.0GluXaa: 0.0 ± 0.0
Phe
2.573PheAla: 2.573 ± 0.209
0.651PheCys: 0.651 ± 0.121
3.745PheAsp: 3.745 ± 0.244
2.589PheGlu: 2.589 ± 0.229
1.726PhePhe: 1.726 ± 0.148
2.947PheGly: 2.947 ± 0.252
0.684PheHis: 0.684 ± 0.144
2.524PheIle: 2.524 ± 0.224
2.605PheLys: 2.605 ± 0.227
3.224PheLeu: 3.224 ± 0.21
0.944PheMet: 0.944 ± 0.148
2.654PheAsn: 2.654 ± 0.26
1.742PhePro: 1.742 ± 0.229
1.4PheGln: 1.4 ± 0.181
1.873PheArg: 1.873 ± 0.166
3.192PheSer: 3.192 ± 0.242
3.029PheThr: 3.029 ± 0.267
3.534PheVal: 3.534 ± 0.224
0.456PheTrp: 0.456 ± 0.098
1.628PheTyr: 1.628 ± 0.144
0.0PheXaa: 0.0 ± 0.0
Gly
4.983GlyAla: 4.983 ± 0.497
0.798GlyCys: 0.798 ± 0.12
4.559GlyAsp: 4.559 ± 0.327
4.25GlyGlu: 4.25 ± 0.29
3.208GlyPhe: 3.208 ± 0.289
7.832GlyGly: 7.832 ± 1.215
1.042GlyHis: 1.042 ± 0.148
5.699GlyIle: 5.699 ± 0.53
4.25GlyLys: 4.25 ± 0.347
4.82GlyLeu: 4.82 ± 0.361
1.645GlyMet: 1.645 ± 0.202
4.462GlyAsn: 4.462 ± 0.375
1.905GlyPro: 1.905 ± 0.213
2.508GlyGln: 2.508 ± 0.234
3.534GlyArg: 3.534 ± 0.276
5.553GlySer: 5.553 ± 0.504
6.53GlyThr: 6.53 ± 0.808
5.439GlyVal: 5.439 ± 0.446
1.107GlyTrp: 1.107 ± 0.133
2.996GlyTyr: 2.996 ± 0.253
0.0GlyXaa: 0.0 ± 0.0
His
0.814HisAla: 0.814 ± 0.103
0.228HisCys: 0.228 ± 0.062
1.042HisAsp: 1.042 ± 0.132
1.058HisGlu: 1.058 ± 0.138
1.058HisPhe: 1.058 ± 0.144
1.189HisGly: 1.189 ± 0.154
0.521HisHis: 0.521 ± 0.106
0.977HisIle: 0.977 ± 0.131
1.058HisLys: 1.058 ± 0.151
1.107HisLeu: 1.107 ± 0.18
0.472HisMet: 0.472 ± 0.094
0.912HisAsn: 0.912 ± 0.122
0.993HisPro: 0.993 ± 0.12
0.798HisGln: 0.798 ± 0.152
0.83HisArg: 0.83 ± 0.115
1.107HisSer: 1.107 ± 0.132
1.156HisThr: 1.156 ± 0.155
0.847HisVal: 0.847 ± 0.137
0.244HisTrp: 0.244 ± 0.068
0.765HisTyr: 0.765 ± 0.133
0.0HisXaa: 0.0 ± 0.0
Ile
3.761IleAla: 3.761 ± 0.266
0.619IleCys: 0.619 ± 0.114
4.901IleAsp: 4.901 ± 0.329
4.576IleGlu: 4.576 ± 0.366
2.182IlePhe: 2.182 ± 0.178
4.934IleGly: 4.934 ± 0.413
1.107IleHis: 1.107 ± 0.151
3.452IleIle: 3.452 ± 0.232
4.25IleLys: 4.25 ± 0.339
4.364IleLeu: 4.364 ± 0.25
1.384IleMet: 1.384 ± 0.161
3.55IleAsn: 3.55 ± 0.279
2.768IlePro: 2.768 ± 0.198
2.198IleGln: 2.198 ± 0.207
3.143IleArg: 3.143 ± 0.263
4.82IleSer: 4.82 ± 0.383
4.804IleThr: 4.804 ± 0.421
4.201IleVal: 4.201 ± 0.279
0.456IleTrp: 0.456 ± 0.096
2.166IleTyr: 2.166 ± 0.169
0.0IleXaa: 0.0 ± 0.0
Lys
3.664LysAla: 3.664 ± 0.392
0.521LysCys: 0.521 ± 0.112
3.924LysAsp: 3.924 ± 0.312
5.146LysGlu: 5.146 ± 0.508
2.915LysPhe: 2.915 ± 0.246
3.534LysGly: 3.534 ± 0.337
1.026LysHis: 1.026 ± 0.155
4.657LysIle: 4.657 ± 0.367
6.057LysLys: 6.057 ± 0.658
5.341LysLeu: 5.341 ± 0.368
1.97LysMet: 1.97 ± 0.268
3.192LysAsn: 3.192 ± 0.286
2.263LysPro: 2.263 ± 0.204
2.133LysGln: 2.133 ± 0.246
2.54LysArg: 2.54 ± 0.237
4.331LysSer: 4.331 ± 0.306
4.087LysThr: 4.087 ± 0.245
4.413LysVal: 4.413 ± 0.281
0.7LysTrp: 0.7 ± 0.123
2.996LysTyr: 2.996 ± 0.254
0.0LysXaa: 0.0 ± 0.0
Leu
4.673LeuAla: 4.673 ± 0.433
0.602LeuCys: 0.602 ± 0.122
5.064LeuAsp: 5.064 ± 0.369
5.227LeuGlu: 5.227 ± 0.318
3.24LeuPhe: 3.24 ± 0.261
4.82LeuGly: 4.82 ± 0.376
1.579LeuHis: 1.579 ± 0.171
4.217LeuIle: 4.217 ± 0.273
5.178LeuLys: 5.178 ± 0.331
4.462LeuLeu: 4.462 ± 0.358
1.482LeuMet: 1.482 ± 0.192
4.364LeuAsn: 4.364 ± 0.238
3.143LeuPro: 3.143 ± 0.211
2.654LeuGln: 2.654 ± 0.266
3.599LeuArg: 3.599 ± 0.276
5.211LeuSer: 5.211 ± 0.271
4.657LeuThr: 4.657 ± 0.387
4.152LeuVal: 4.152 ± 0.272
0.765LeuTrp: 0.765 ± 0.13
2.85LeuTyr: 2.85 ± 0.211
0.0LeuXaa: 0.0 ± 0.0
Met
1.563MetAla: 1.563 ± 0.191
0.326MetCys: 0.326 ± 0.084
1.124MetAsp: 1.124 ± 0.142
1.482MetGlu: 1.482 ± 0.207
1.042MetPhe: 1.042 ± 0.153
1.368MetGly: 1.368 ± 0.197
0.407MetHis: 0.407 ± 0.1
1.514MetIle: 1.514 ± 0.187
2.117MetLys: 2.117 ± 0.267
1.466MetLeu: 1.466 ± 0.176
0.668MetMet: 0.668 ± 0.119
1.368MetAsn: 1.368 ± 0.162
1.026MetPro: 1.026 ± 0.158
0.798MetGln: 0.798 ± 0.151
1.14MetArg: 1.14 ± 0.138
1.987MetSer: 1.987 ± 0.212
1.352MetThr: 1.352 ± 0.161
1.4MetVal: 1.4 ± 0.141
0.228MetTrp: 0.228 ± 0.074
0.798MetTyr: 0.798 ± 0.126
0.0MetXaa: 0.0 ± 0.0
Asn
3.273AsnAla: 3.273 ± 0.29
0.456AsnCys: 0.456 ± 0.078
3.664AsnAsp: 3.664 ± 0.329
3.175AsnGlu: 3.175 ± 0.22
2.752AsnPhe: 2.752 ± 0.214
3.729AsnGly: 3.729 ± 0.32
1.042AsnHis: 1.042 ± 0.136
3.875AsnIle: 3.875 ± 0.217
3.289AsnLys: 3.289 ± 0.25
4.804AsnLeu: 4.804 ± 0.269
1.026AsnMet: 1.026 ± 0.153
2.898AsnAsn: 2.898 ± 0.241
3.192AsnPro: 3.192 ± 0.276
1.905AsnGln: 1.905 ± 0.191
2.41AsnArg: 2.41 ± 0.173
3.713AsnSer: 3.713 ± 0.331
3.403AsnThr: 3.403 ± 0.294
4.266AsnVal: 4.266 ± 0.287
0.619AsnTrp: 0.619 ± 0.097
2.117AsnTyr: 2.117 ± 0.224
0.0AsnXaa: 0.0 ± 0.0
Pro
2.622ProAla: 2.622 ± 0.232
0.342ProCys: 0.342 ± 0.066
2.605ProAsp: 2.605 ± 0.229
3.224ProGlu: 3.224 ± 0.333
1.498ProPhe: 1.498 ± 0.16
3.094ProGly: 3.094 ± 0.288
0.798ProHis: 0.798 ± 0.104
2.003ProIle: 2.003 ± 0.203
2.41ProLys: 2.41 ± 0.267
2.638ProLeu: 2.638 ± 0.22
0.765ProMet: 0.765 ± 0.119
2.117ProAsn: 2.117 ± 0.188
1.645ProPro: 1.645 ± 0.273
1.498ProGln: 1.498 ± 0.146
1.498ProArg: 1.498 ± 0.172
2.817ProSer: 2.817 ± 0.22
3.175ProThr: 3.175 ± 0.182
2.947ProVal: 2.947 ± 0.264
0.472ProTrp: 0.472 ± 0.098
1.726ProTyr: 1.726 ± 0.177
0.0ProXaa: 0.0 ± 0.0
Gln
1.905GlnAla: 1.905 ± 0.193
0.179GlnCys: 0.179 ± 0.05
2.133GlnAsp: 2.133 ± 0.189
2.833GlnGlu: 2.833 ± 0.188
1.661GlnPhe: 1.661 ± 0.15
2.182GlnGly: 2.182 ± 0.192
0.521GlnHis: 0.521 ± 0.095
2.394GlnIle: 2.394 ± 0.184
2.508GlnLys: 2.508 ± 0.256
2.931GlnLeu: 2.931 ± 0.218
0.961GlnMet: 0.961 ± 0.121
1.807GlnAsn: 1.807 ± 0.169
1.091GlnPro: 1.091 ± 0.137
1.498GlnGln: 1.498 ± 0.196
1.498GlnArg: 1.498 ± 0.165
2.247GlnSer: 2.247 ± 0.201
2.215GlnThr: 2.215 ± 0.175
2.149GlnVal: 2.149 ± 0.175
0.423GlnTrp: 0.423 ± 0.09
1.628GlnTyr: 1.628 ± 0.165
0.0GlnXaa: 0.0 ± 0.0
Arg
2.394ArgAla: 2.394 ± 0.201
0.521ArgCys: 0.521 ± 0.09
2.345ArgAsp: 2.345 ± 0.151
2.964ArgGlu: 2.964 ± 0.238
2.003ArgPhe: 2.003 ± 0.205
3.029ArgGly: 3.029 ± 0.272
0.733ArgHis: 0.733 ± 0.111
3.029ArgIle: 3.029 ± 0.258
3.045ArgLys: 3.045 ± 0.239
2.947ArgLeu: 2.947 ± 0.23
1.27ArgMet: 1.27 ± 0.171
2.263ArgAsn: 2.263 ± 0.2
1.563ArgPro: 1.563 ± 0.173
1.352ArgGln: 1.352 ± 0.135
2.361ArgArg: 2.361 ± 0.241
2.557ArgSer: 2.557 ± 0.248
2.329ArgThr: 2.329 ± 0.207
3.24ArgVal: 3.24 ± 0.332
0.586ArgTrp: 0.586 ± 0.111
2.231ArgTyr: 2.231 ± 0.188
0.0ArgXaa: 0.0 ± 0.0
Ser
4.983SerAla: 4.983 ± 0.373
0.521SerCys: 0.521 ± 0.116
4.738SerAsp: 4.738 ± 0.378
3.713SerGlu: 3.713 ± 0.267
3.159SerPhe: 3.159 ± 0.243
6.676SerGly: 6.676 ± 0.616
1.01SerHis: 1.01 ± 0.131
4.397SerIle: 4.397 ± 0.26
3.582SerLys: 3.582 ± 0.286
4.869SerLeu: 4.869 ± 0.384
1.71SerMet: 1.71 ± 0.222
3.989SerAsn: 3.989 ± 0.349
2.247SerPro: 2.247 ± 0.196
2.28SerGln: 2.28 ± 0.205
2.296SerArg: 2.296 ± 0.19
4.722SerSer: 4.722 ± 0.377
5.129SerThr: 5.129 ± 0.599
5.162SerVal: 5.162 ± 0.388
0.749SerTrp: 0.749 ± 0.13
2.719SerTyr: 2.719 ± 0.241
0.0SerXaa: 0.0 ± 0.0
Thr
4.673ThrAla: 4.673 ± 0.35
0.423ThrCys: 0.423 ± 0.092
3.794ThrAsp: 3.794 ± 0.256
4.25ThrGlu: 4.25 ± 0.291
3.778ThrPhe: 3.778 ± 0.418
6.106ThrGly: 6.106 ± 0.786
0.977ThrHis: 0.977 ± 0.132
4.283ThrIle: 4.283 ± 0.402
3.941ThrLys: 3.941 ± 0.257
5.162ThrLeu: 5.162 ± 0.361
1.352ThrMet: 1.352 ± 0.141
3.859ThrAsn: 3.859 ± 0.355
3.078ThrPro: 3.078 ± 0.234
2.198ThrGln: 2.198 ± 0.172
2.394ThrArg: 2.394 ± 0.191
4.625ThrSer: 4.625 ± 0.606
5.813ThrThr: 5.813 ± 0.602
5.162ThrVal: 5.162 ± 0.441
0.847ThrTrp: 0.847 ± 0.113
2.394ThrTyr: 2.394 ± 0.196
0.0ThrXaa: 0.0 ± 0.0
Val
4.771ValAla: 4.771 ± 0.324
0.586ValCys: 0.586 ± 0.123
5.015ValAsp: 5.015 ± 0.335
4.364ValGlu: 4.364 ± 0.294
2.215ValPhe: 2.215 ± 0.209
6.302ValGly: 6.302 ± 0.743
1.14ValHis: 1.14 ± 0.143
3.924ValIle: 3.924 ± 0.196
4.152ValLys: 4.152 ± 0.261
4.103ValLeu: 4.103 ± 0.255
1.417ValMet: 1.417 ± 0.143
3.892ValAsn: 3.892 ± 0.268
2.768ValPro: 2.768 ± 0.238
2.557ValGln: 2.557 ± 0.222
3.175ValArg: 3.175 ± 0.207
5.194ValSer: 5.194 ± 0.436
4.95ValThr: 4.95 ± 0.415
4.641ValVal: 4.641 ± 0.292
0.668ValTrp: 0.668 ± 0.131
2.947ValTyr: 2.947 ± 0.192
0.0ValXaa: 0.0 ± 0.0
Trp
0.57TrpAla: 0.57 ± 0.093
0.244TrpCys: 0.244 ± 0.058
0.733TrpAsp: 0.733 ± 0.142
0.912TrpGlu: 0.912 ± 0.143
0.619TrpPhe: 0.619 ± 0.107
0.798TrpGly: 0.798 ± 0.134
0.358TrpHis: 0.358 ± 0.078
0.651TrpIle: 0.651 ± 0.119
0.765TrpLys: 0.765 ± 0.133
0.961TrpLeu: 0.961 ± 0.166
0.244TrpMet: 0.244 ± 0.067
0.765TrpAsn: 0.765 ± 0.149
0.309TrpPro: 0.309 ± 0.069
0.423TrpGln: 0.423 ± 0.073
0.586TrpArg: 0.586 ± 0.106
0.635TrpSer: 0.635 ± 0.1
0.782TrpThr: 0.782 ± 0.139
0.798TrpVal: 0.798 ± 0.134
0.114TrpTrp: 0.114 ± 0.05
0.472TrpTyr: 0.472 ± 0.103
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.263TyrAla: 2.263 ± 0.154
0.521TyrCys: 0.521 ± 0.091
3.078TyrAsp: 3.078 ± 0.196
2.719TyrGlu: 2.719 ± 0.204
1.807TyrPhe: 1.807 ± 0.163
2.41TyrGly: 2.41 ± 0.163
0.765TyrHis: 0.765 ± 0.118
2.882TyrIle: 2.882 ± 0.244
2.247TyrLys: 2.247 ± 0.186
3.078TyrLeu: 3.078 ± 0.21
0.814TyrMet: 0.814 ± 0.134
2.491TyrAsn: 2.491 ± 0.204
1.807TyrPro: 1.807 ± 0.184
1.742TyrGln: 1.742 ± 0.192
1.856TyrArg: 1.856 ± 0.162
2.882TyrSer: 2.882 ± 0.214
2.312TyrThr: 2.312 ± 0.162
2.719TyrVal: 2.719 ± 0.198
0.521TyrTrp: 0.521 ± 0.116
1.905TyrTyr: 1.905 ± 0.208
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 267 proteins (61413 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski