Amino acid dipepetide frequency for Nocardioides sp. MAH-18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.744AlaAla: 20.744 ± 0.195
0.985AlaCys: 0.985 ± 0.028
8.813AlaAsp: 8.813 ± 0.087
8.246AlaGlu: 8.246 ± 0.099
3.568AlaPhe: 3.568 ± 0.055
12.796AlaGly: 12.796 ± 0.111
2.599AlaHis: 2.599 ± 0.04
4.618AlaIle: 4.618 ± 0.058
2.408AlaLys: 2.408 ± 0.055
13.567AlaLeu: 13.567 ± 0.142
2.707AlaMet: 2.707 ± 0.05
2.042AlaAsn: 2.042 ± 0.037
6.197AlaPro: 6.197 ± 0.089
3.48AlaGln: 3.48 ± 0.051
9.46AlaArg: 9.46 ± 0.095
6.372AlaSer: 6.372 ± 0.072
7.853AlaThr: 7.853 ± 0.079
12.241AlaVal: 12.241 ± 0.129
2.07AlaTrp: 2.07 ± 0.04
2.689AlaTyr: 2.689 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
0.917CysAla: 0.917 ± 0.028
0.083CysCys: 0.083 ± 0.008
0.487CysAsp: 0.487 ± 0.022
0.345CysGlu: 0.345 ± 0.015
0.232CysPhe: 0.232 ± 0.015
0.831CysGly: 0.831 ± 0.029
0.177CysHis: 0.177 ± 0.012
0.185CysIle: 0.185 ± 0.01
0.097CysLys: 0.097 ± 0.007
0.677CysLeu: 0.677 ± 0.023
0.105CysMet: 0.105 ± 0.009
0.134CysAsn: 0.134 ± 0.01
0.433CysPro: 0.433 ± 0.02
0.18CysGln: 0.18 ± 0.011
0.535CysArg: 0.535 ± 0.02
0.428CysSer: 0.428 ± 0.017
0.485CysThr: 0.485 ± 0.021
0.614CysVal: 0.614 ± 0.021
0.121CysTrp: 0.121 ± 0.01
0.166CysTyr: 0.166 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
8.416AspAla: 8.416 ± 0.083
0.381AspCys: 0.381 ± 0.018
4.821AspAsp: 4.821 ± 0.069
4.422AspGlu: 4.422 ± 0.062
1.671AspPhe: 1.671 ± 0.036
6.736AspGly: 6.736 ± 0.083
1.552AspHis: 1.552 ± 0.031
1.779AspIle: 1.779 ± 0.034
1.048AspLys: 1.048 ± 0.032
7.49AspLeu: 7.49 ± 0.073
0.839AspMet: 0.839 ± 0.022
0.987AspAsn: 0.987 ± 0.024
4.744AspPro: 4.744 ± 0.062
1.917AspGln: 1.917 ± 0.036
5.165AspArg: 5.165 ± 0.066
2.326AspSer: 2.326 ± 0.04
2.921AspThr: 2.921 ± 0.052
6.304AspVal: 6.304 ± 0.073
1.037AspTrp: 1.037 ± 0.028
1.234AspTyr: 1.234 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
7.018GluAla: 7.018 ± 0.097
0.332GluCys: 0.332 ± 0.015
3.137GluAsp: 3.137 ± 0.048
3.258GluGlu: 3.258 ± 0.062
1.425GluPhe: 1.425 ± 0.03
4.12GluGly: 4.12 ± 0.054
1.638GluHis: 1.638 ± 0.034
2.435GluIle: 2.435 ± 0.045
1.212GluLys: 1.212 ± 0.032
6.517GluLeu: 6.517 ± 0.078
1.065GluMet: 1.065 ± 0.024
0.843GluAsn: 0.843 ± 0.026
3.266GluPro: 3.266 ± 0.057
2.305GluGln: 2.305 ± 0.047
5.184GluArg: 5.184 ± 0.067
2.631GluSer: 2.631 ± 0.04
3.02GluThr: 3.02 ± 0.042
5.635GluVal: 5.635 ± 0.062
0.825GluTrp: 0.825 ± 0.026
0.943GluTyr: 0.943 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
3.663PheAla: 3.663 ± 0.054
0.255PheCys: 0.255 ± 0.014
2.117PheAsp: 2.117 ± 0.035
1.648PheGlu: 1.648 ± 0.037
0.908PhePhe: 0.908 ± 0.027
3.032PheGly: 3.032 ± 0.052
0.585PheHis: 0.585 ± 0.02
0.773PheIle: 0.773 ± 0.025
0.47PheLys: 0.47 ± 0.02
2.57PheLeu: 2.57 ± 0.045
0.407PheMet: 0.407 ± 0.018
0.573PheAsn: 0.573 ± 0.022
1.316PhePro: 1.316 ± 0.035
0.71PheGln: 0.71 ± 0.02
1.723PheArg: 1.723 ± 0.037
1.459PheSer: 1.459 ± 0.032
1.857PheThr: 1.857 ± 0.038
2.679PheVal: 2.679 ± 0.05
0.444PheTrp: 0.444 ± 0.017
0.622PheTyr: 0.622 ± 0.021
0.0PheXaa: 0.0 ± 0.0
Gly
10.737GlyAla: 10.737 ± 0.092
0.833GlyCys: 0.833 ± 0.026
5.591GlyAsp: 5.591 ± 0.073
4.963GlyGlu: 4.963 ± 0.055
3.013GlyPhe: 3.013 ± 0.048
8.205GlyGly: 8.205 ± 0.114
2.133GlyHis: 2.133 ± 0.041
3.819GlyIle: 3.819 ± 0.054
2.04GlyLys: 2.04 ± 0.046
9.474GlyLeu: 9.474 ± 0.096
1.951GlyMet: 1.951 ± 0.038
1.682GlyAsn: 1.682 ± 0.045
4.632GlyPro: 4.632 ± 0.064
2.526GlyGln: 2.526 ± 0.047
7.291GlyArg: 7.291 ± 0.075
5.356GlySer: 5.356 ± 0.057
5.886GlyThr: 5.886 ± 0.077
8.198GlyVal: 8.198 ± 0.083
1.737GlyTrp: 1.737 ± 0.038
2.213GlyTyr: 2.213 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
2.627HisAla: 2.627 ± 0.038
0.182HisCys: 0.182 ± 0.011
1.562HisAsp: 1.562 ± 0.035
1.282HisGlu: 1.282 ± 0.029
0.571HisPhe: 0.571 ± 0.019
2.247HisGly: 2.247 ± 0.036
0.697HisHis: 0.697 ± 0.027
0.525HisIle: 0.525 ± 0.019
0.298HisLys: 0.298 ± 0.015
2.369HisLeu: 2.369 ± 0.046
0.289HisMet: 0.289 ± 0.013
0.326HisAsn: 0.326 ± 0.015
1.592HisPro: 1.592 ± 0.032
0.666HisGln: 0.666 ± 0.022
1.905HisArg: 1.905 ± 0.037
0.809HisSer: 0.809 ± 0.026
1.057HisThr: 1.057 ± 0.026
2.001HisVal: 2.001 ± 0.039
0.345HisTrp: 0.345 ± 0.015
0.457HisTyr: 0.457 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
5.138IleAla: 5.138 ± 0.066
0.276IleCys: 0.276 ± 0.017
2.653IleAsp: 2.653 ± 0.046
2.239IleGlu: 2.239 ± 0.051
0.858IlePhe: 0.858 ± 0.029
3.801IleGly: 3.801 ± 0.061
0.625IleHis: 0.625 ± 0.021
0.962IleIle: 0.962 ± 0.031
0.683IleLys: 0.683 ± 0.023
2.564IleLeu: 2.564 ± 0.045
0.456IleMet: 0.456 ± 0.019
0.773IleAsn: 0.773 ± 0.023
1.758IlePro: 1.758 ± 0.036
0.787IleGln: 0.787 ± 0.02
2.264IleArg: 2.264 ± 0.042
1.836IleSer: 1.836 ± 0.042
2.245IleThr: 2.245 ± 0.047
3.275IleVal: 3.275 ± 0.053
0.434IleTrp: 0.434 ± 0.018
0.62IleTyr: 0.62 ± 0.022
0.0IleXaa: 0.0 ± 0.0
Lys
2.416LysAla: 2.416 ± 0.053
0.093LysCys: 0.093 ± 0.009
1.062LysAsp: 1.062 ± 0.029
0.922LysGlu: 0.922 ± 0.028
0.424LysPhe: 0.424 ± 0.017
1.447LysGly: 1.447 ± 0.034
0.427LysHis: 0.427 ± 0.019
0.735LysIle: 0.735 ± 0.027
0.652LysLys: 0.652 ± 0.035
1.634LysLeu: 1.634 ± 0.04
0.337LysMet: 0.337 ± 0.016
0.388LysAsn: 0.388 ± 0.016
1.175LysPro: 1.175 ± 0.034
0.656LysGln: 0.656 ± 0.027
1.33LysArg: 1.33 ± 0.033
1.002LysSer: 1.002 ± 0.032
1.118LysThr: 1.118 ± 0.029
1.886LysVal: 1.886 ± 0.041
0.206LysTrp: 0.206 ± 0.012
0.397LysTyr: 0.397 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
15.031LeuAla: 15.031 ± 0.127
0.718LeuCys: 0.718 ± 0.022
7.37LeuAsp: 7.37 ± 0.087
5.293LeuGlu: 5.293 ± 0.07
2.428LeuPhe: 2.428 ± 0.047
9.686LeuGly: 9.686 ± 0.079
2.019LeuHis: 2.019 ± 0.035
2.865LeuIle: 2.865 ± 0.058
1.626LeuLys: 1.626 ± 0.038
10.742LeuLeu: 10.742 ± 0.122
1.63LeuMet: 1.63 ± 0.032
1.565LeuAsn: 1.565 ± 0.038
5.781LeuPro: 5.781 ± 0.065
2.307LeuGln: 2.307 ± 0.04
7.822LeuArg: 7.822 ± 0.088
5.27LeuSer: 5.27 ± 0.067
6.484LeuThr: 6.484 ± 0.07
10.815LeuVal: 10.815 ± 0.1
1.209LeuTrp: 1.209 ± 0.031
1.565LeuTyr: 1.565 ± 0.036
0.0LeuXaa: 0.0 ± 0.0
Met
2.153MetAla: 2.153 ± 0.038
0.141MetCys: 0.141 ± 0.011
0.871MetAsp: 0.871 ± 0.024
0.751MetGlu: 0.751 ± 0.025
0.489MetPhe: 0.489 ± 0.019
1.377MetGly: 1.377 ± 0.029
0.382MetHis: 0.382 ± 0.017
0.645MetIle: 0.645 ± 0.022
0.418MetLys: 0.418 ± 0.017
1.854MetLeu: 1.854 ± 0.035
0.328MetMet: 0.328 ± 0.017
0.408MetAsn: 0.408 ± 0.016
1.118MetPro: 1.118 ± 0.031
0.449MetGln: 0.449 ± 0.018
1.411MetArg: 1.411 ± 0.032
1.467MetSer: 1.467 ± 0.033
1.747MetThr: 1.747 ± 0.04
1.53MetVal: 1.53 ± 0.033
0.215MetTrp: 0.215 ± 0.014
0.281MetTyr: 0.281 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.181AsnAla: 2.181 ± 0.04
0.134AsnCys: 0.134 ± 0.009
1.054AsnAsp: 1.054 ± 0.031
0.849AsnGlu: 0.849 ± 0.024
0.488AsnPhe: 0.488 ± 0.02
1.647AsnGly: 1.647 ± 0.035
0.377AsnHis: 0.377 ± 0.018
0.633AsnIle: 0.633 ± 0.02
0.353AsnLys: 0.353 ± 0.018
1.758AsnLeu: 1.758 ± 0.035
0.256AsnMet: 0.256 ± 0.014
0.402AsnAsn: 0.402 ± 0.018
1.401AsnPro: 1.401 ± 0.035
0.539AsnGln: 0.539 ± 0.021
1.235AsnArg: 1.235 ± 0.029
0.739AsnSer: 0.739 ± 0.024
0.951AsnThr: 0.951 ± 0.027
1.551AsnVal: 1.551 ± 0.034
0.251AsnTrp: 0.251 ± 0.012
0.364AsnTyr: 0.364 ± 0.018
0.0AsnXaa: 0.0 ± 0.0
Pro
7.699ProAla: 7.699 ± 0.091
0.299ProCys: 0.299 ± 0.014
4.638ProAsp: 4.638 ± 0.058
3.96ProGlu: 3.96 ± 0.054
1.557ProPhe: 1.557 ± 0.033
5.658ProGly: 5.658 ± 0.07
1.165ProHis: 1.165 ± 0.031
1.744ProIle: 1.744 ± 0.038
0.971ProLys: 0.971 ± 0.029
4.778ProLeu: 4.778 ± 0.067
1.048ProMet: 1.048 ± 0.03
0.85ProAsn: 0.85 ± 0.026
2.988ProPro: 2.988 ± 0.058
1.397ProGln: 1.397 ± 0.03
3.557ProArg: 3.557 ± 0.057
3.008ProSer: 3.008 ± 0.055
3.725ProThr: 3.725 ± 0.058
5.334ProVal: 5.334 ± 0.058
0.948ProTrp: 0.948 ± 0.024
1.211ProTyr: 1.211 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
3.523GlnAla: 3.523 ± 0.047
0.16GlnCys: 0.16 ± 0.011
1.33GlnAsp: 1.33 ± 0.028
1.365GlnGlu: 1.365 ± 0.03
0.679GlnPhe: 0.679 ± 0.019
2.048GlnGly: 2.048 ± 0.042
0.71GlnHis: 0.71 ± 0.021
1.05GlnIle: 1.05 ± 0.028
0.496GlnLys: 0.496 ± 0.019
3.005GlnLeu: 3.005 ± 0.048
0.569GlnMet: 0.569 ± 0.019
0.468GlnAsn: 0.468 ± 0.019
1.702GlnPro: 1.702 ± 0.035
1.209GlnGln: 1.209 ± 0.035
2.453GlnArg: 2.453 ± 0.041
1.188GlnSer: 1.188 ± 0.031
1.435GlnThr: 1.435 ± 0.031
2.991GlnVal: 2.991 ± 0.049
0.422GlnTrp: 0.422 ± 0.018
0.529GlnTyr: 0.529 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
9.186ArgAla: 9.186 ± 0.096
0.528ArgCys: 0.528 ± 0.02
4.468ArgAsp: 4.468 ± 0.06
4.382ArgGlu: 4.382 ± 0.063
2.245ArgPhe: 2.245 ± 0.04
5.68ArgGly: 5.68 ± 0.074
1.848ArgHis: 1.848 ± 0.036
3.051ArgIle: 3.051 ± 0.042
1.354ArgLys: 1.354 ± 0.033
8.204ArgLeu: 8.204 ± 0.089
1.739ArgMet: 1.739 ± 0.034
1.268ArgAsn: 1.268 ± 0.028
4.335ArgPro: 4.335 ± 0.062
2.099ArgGln: 2.099 ± 0.042
7.373ArgArg: 7.373 ± 0.098
4.157ArgSer: 4.157 ± 0.059
4.747ArgThr: 4.747 ± 0.063
6.564ArgVal: 6.564 ± 0.068
1.442ArgTrp: 1.442 ± 0.034
1.62ArgTyr: 1.62 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
6.436SerAla: 6.436 ± 0.075
0.341SerCys: 0.341 ± 0.016
3.008SerAsp: 3.008 ± 0.049
2.458SerGlu: 2.458 ± 0.042
1.641SerPhe: 1.641 ± 0.031
5.514SerGly: 5.514 ± 0.057
0.978SerHis: 0.978 ± 0.022
1.856SerIle: 1.856 ± 0.046
0.926SerLys: 0.926 ± 0.028
4.839SerLeu: 4.839 ± 0.065
1.213SerMet: 1.213 ± 0.033
0.91SerAsn: 0.91 ± 0.026
2.973SerPro: 2.973 ± 0.041
1.325SerGln: 1.325 ± 0.033
3.647SerArg: 3.647 ± 0.059
3.02SerSer: 3.02 ± 0.051
3.394SerThr: 3.394 ± 0.05
4.436SerVal: 4.436 ± 0.051
0.954SerTrp: 0.954 ± 0.025
1.307SerTyr: 1.307 ± 0.03
0.0SerXaa: 0.0 ± 0.0
Thr
7.706ThrAla: 7.706 ± 0.092
0.475ThrCys: 0.475 ± 0.02
3.656ThrAsp: 3.656 ± 0.05
3.018ThrGlu: 3.018 ± 0.049
1.951ThrPhe: 1.951 ± 0.041
6.215ThrGly: 6.215 ± 0.078
1.173ThrHis: 1.173 ± 0.029
2.471ThrIle: 2.471 ± 0.043
1.098ThrLys: 1.098 ± 0.031
5.859ThrLeu: 5.859 ± 0.063
1.094ThrMet: 1.094 ± 0.03
1.131ThrAsn: 1.131 ± 0.031
3.921ThrPro: 3.921 ± 0.063
1.429ThrGln: 1.429 ± 0.027
3.809ThrArg: 3.809 ± 0.05
3.4ThrSer: 3.4 ± 0.05
4.253ThrThr: 4.253 ± 0.063
6.076ThrVal: 6.076 ± 0.076
1.083ThrTrp: 1.083 ± 0.026
1.563ThrTyr: 1.563 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
13.318ValAla: 13.318 ± 0.134
0.711ValCys: 0.711 ± 0.025
6.596ValAsp: 6.596 ± 0.077
5.666ValGlu: 5.666 ± 0.065
2.496ValPhe: 2.496 ± 0.051
8.151ValGly: 8.151 ± 0.094
1.99ValHis: 1.99 ± 0.037
3.072ValIle: 3.072 ± 0.046
1.564ValLys: 1.564 ± 0.038
10.281ValLeu: 10.281 ± 0.106
1.481ValMet: 1.481 ± 0.033
1.71ValAsn: 1.71 ± 0.04
5.351ValPro: 5.351 ± 0.065
2.234ValGln: 2.234 ± 0.036
7.207ValArg: 7.207 ± 0.072
4.723ValSer: 4.723 ± 0.064
6.105ValThr: 6.105 ± 0.075
11.052ValVal: 11.052 ± 0.124
1.177ValTrp: 1.177 ± 0.03
1.526ValTyr: 1.526 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.68TrpAla: 1.68 ± 0.036
0.162TrpCys: 0.162 ± 0.011
0.843TrpAsp: 0.843 ± 0.025
0.703TrpGlu: 0.703 ± 0.024
0.541TrpPhe: 0.541 ± 0.02
1.125TrpGly: 1.125 ± 0.033
0.378TrpHis: 0.378 ± 0.017
0.593TrpIle: 0.593 ± 0.019
0.283TrpLys: 0.283 ± 0.016
1.884TrpLeu: 1.884 ± 0.04
0.299TrpMet: 0.299 ± 0.013
0.318TrpAsn: 0.318 ± 0.014
0.811TrpPro: 0.811 ± 0.024
0.559TrpGln: 0.559 ± 0.017
1.346TrpArg: 1.346 ± 0.029
1.049TrpSer: 1.049 ± 0.028
1.046TrpThr: 1.046 ± 0.029
1.302TrpVal: 1.302 ± 0.027
0.416TrpTrp: 0.416 ± 0.02
0.326TrpTyr: 0.326 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.868TyrAla: 2.868 ± 0.047
0.145TyrCys: 0.145 ± 0.01
1.712TyrAsp: 1.712 ± 0.039
1.042TyrGlu: 1.042 ± 0.027
0.629TyrPhe: 0.629 ± 0.022
1.965TyrGly: 1.965 ± 0.042
0.353TyrHis: 0.353 ± 0.015
0.436TyrIle: 0.436 ± 0.018
0.311TyrLys: 0.311 ± 0.015
2.161TyrLeu: 2.161 ± 0.042
0.219TyrMet: 0.219 ± 0.013
0.384TyrAsn: 0.384 ± 0.016
1.041TyrPro: 1.041 ± 0.025
0.552TyrGln: 0.552 ± 0.021
1.573TyrArg: 1.573 ± 0.036
0.937TyrSer: 0.937 ± 0.028
1.049TyrThr: 1.049 ± 0.031
1.93TyrVal: 1.93 ± 0.032
0.327TyrTrp: 0.327 ± 0.015
0.421TyrTyr: 0.421 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4572 proteins (1479584 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski