Hubei sobemo-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KER8|A0A1L3KER8_9VIRU Uncharacterized protein OS=Hubei sobemo-like virus 1 OX=1923194 PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84IAATNKK8 pKa = 9.93EE9 pKa = 4.25LLEE12 pKa = 4.11LHH14 pKa = 6.32WSLVEE19 pKa = 3.89NAAVARR25 pKa = 11.84LQLLEE30 pKa = 4.13EE31 pKa = 4.17VDD33 pKa = 4.23YY34 pKa = 11.56EE35 pKa = 4.38DD36 pKa = 4.7LRR38 pKa = 11.84AMSASEE44 pKa = 4.04LVEE47 pKa = 4.22GGYY50 pKa = 9.06TDD52 pKa = 3.46EE53 pKa = 4.25VRR55 pKa = 11.84VFVKK59 pKa = 10.98NEE61 pKa = 3.37LHH63 pKa = 6.44SRR65 pKa = 11.84AKK67 pKa = 9.71VAEE70 pKa = 3.79GRR72 pKa = 11.84MRR74 pKa = 11.84LIMSVSVVDD83 pKa = 4.17QIVEE87 pKa = 4.15RR88 pKa = 11.84VLNSAQNQLEE98 pKa = 3.93ISKK101 pKa = 10.02WEE103 pKa = 4.35TISSKK108 pKa = 10.72PGMGLDD114 pKa = 4.14DD115 pKa = 5.75DD116 pKa = 4.9SLFSIQEE123 pKa = 3.97QVHH126 pKa = 5.91KK127 pKa = 10.43LGSGVGDD134 pKa = 3.82PVSSDD139 pKa = 2.33ISGFDD144 pKa = 3.03WSVPQWALDD153 pKa = 3.27LDD155 pKa = 4.0ARR157 pKa = 11.84VRR159 pKa = 11.84AALSGAPTWLRR170 pKa = 11.84VHH172 pKa = 6.74EE173 pKa = 4.41KK174 pKa = 10.55RR175 pKa = 11.84AVCLGLSQLVFSDD188 pKa = 3.88GVVWDD193 pKa = 3.65QLEE196 pKa = 4.17PGIQKK201 pKa = 10.25SGSYY205 pKa = 8.29NTSSTNSRR213 pKa = 11.84IRR215 pKa = 11.84VMLAWLVARR224 pKa = 11.84RR225 pKa = 11.84IPQSRR230 pKa = 11.84YY231 pKa = 8.66GGRR234 pKa = 11.84DD235 pKa = 3.1ALEE238 pKa = 4.37GGGRR242 pKa = 11.84AMAMGDD248 pKa = 3.63DD249 pKa = 4.64AIEE252 pKa = 4.19TTTCMEE258 pKa = 4.25LLKK261 pKa = 10.69PNYY264 pKa = 9.06EE265 pKa = 3.84RR266 pKa = 11.84LGFVLKK272 pKa = 10.34EE273 pKa = 3.71VSRR276 pKa = 11.84DD277 pKa = 3.3IEE279 pKa = 4.06FCAYY283 pKa = 10.32AFDD286 pKa = 4.48LAQGYY291 pKa = 9.28RR292 pKa = 11.84PLRR295 pKa = 11.84VVKK298 pKa = 9.74MVAGLLRR305 pKa = 11.84TAVRR309 pKa = 11.84DD310 pKa = 3.71EE311 pKa = 3.85QHH313 pKa = 6.42EE314 pKa = 4.38EE315 pKa = 3.9EE316 pKa = 4.7LRR318 pKa = 11.84VALEE322 pKa = 3.79YY323 pKa = 10.63EE324 pKa = 4.38LRR326 pKa = 11.84HH327 pKa = 6.09SDD329 pKa = 3.0KK330 pKa = 10.96QAWAKK335 pKa = 10.26HH336 pKa = 5.15VIRR339 pKa = 11.84ASGWGARR346 pKa = 11.84KK347 pKa = 9.44

Molecular weight: 38.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEV9|A0A1L3KEV9_9VIRU Peptidase S39 domain-containing protein OS=Hubei sobemo-like virus 1 OX=1923194 PE=4 SV=1
MM1 pKa = 6.97VKK3 pKa = 10.35KK4 pKa = 10.42IRR6 pKa = 11.84VRR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.0KK11 pKa = 9.93QAAKK15 pKa = 10.34APQPRR20 pKa = 11.84KK21 pKa = 10.07GVAQRR26 pKa = 11.84AMVQRR31 pKa = 11.84SVLDD35 pKa = 3.29SGAAAWARR43 pKa = 11.84LLADD47 pKa = 4.11PCAAPLTYY55 pKa = 9.82PCYY58 pKa = 8.87PTGTGGSVLMRR69 pKa = 11.84FEE71 pKa = 4.91SDD73 pKa = 3.51FLFATGATEE82 pKa = 3.71VAGIFGFCPGSLSGFSNATPLTSDD106 pKa = 2.67ILGTVLTLQSTSIPGFTFINANTNSYY132 pKa = 10.51RR133 pKa = 11.84PVAACMQVMYY143 pKa = 10.25PGTEE147 pKa = 4.05LNRR150 pKa = 11.84SGVVGVGICSGDD162 pKa = 3.37VLLRR166 pKa = 11.84NVNTVGGGSNINTNASEE183 pKa = 4.03VRR185 pKa = 11.84TMCQHH190 pKa = 5.71VEE192 pKa = 3.96RR193 pKa = 11.84MPTTVVEE200 pKa = 4.66IKK202 pKa = 9.57WFPGTADD209 pKa = 3.59EE210 pKa = 5.12EE211 pKa = 4.61NNSTQGLKK219 pKa = 10.68LGTLTDD225 pKa = 3.25IEE227 pKa = 4.32GRR229 pKa = 11.84NTIFMSASGFPVSTGIRR246 pKa = 11.84IRR248 pKa = 11.84MVAVYY253 pKa = 9.1EE254 pKa = 4.06LSFGAGAGQIAGSVAPTSGNTPAQVVRR281 pKa = 11.84ALTNRR286 pKa = 11.84DD287 pKa = 3.27ADD289 pKa = 3.83WYY291 pKa = 9.75ISSAAKK297 pKa = 9.82IGKK300 pKa = 9.43AVGNTISYY308 pKa = 10.04VATGYY313 pKa = 10.27KK314 pKa = 9.91AASAFAAGLALII326 pKa = 4.85

Molecular weight: 34.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1210

326

537

403.3

43.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.0 ± 0.84

1.157 ± 0.254

4.132 ± 0.771

5.455 ± 1.214

3.554 ± 0.581

8.264 ± 0.918

1.322 ± 0.4

4.628 ± 0.349

4.628 ± 0.625

8.512 ± 1.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.479 ± 0.228

2.562 ± 0.899

5.702 ± 1.514

2.975 ± 0.442

6.116 ± 0.85

10.083 ± 1.265

5.372 ± 1.399

9.091 ± 0.21

1.24 ± 0.515

2.727 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski