Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Fumigati; Aspergillus fumigatus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9647 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4WMB8|Q4WMB8_ASPFU Ceramide synthase membrane component (LAG1) putative OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=AFUA_6G10460 PE=3 SV=1
MM1 pKa = 7.39HH2 pKa = 7.57LALSLLALTATSTLAAPVNSVSQKK26 pKa = 9.84QDD28 pKa = 2.43SAAPIRR34 pKa = 11.84TSKK37 pKa = 10.79LLAPISHH44 pKa = 7.11PAKK47 pKa = 10.42EE48 pKa = 4.11EE49 pKa = 3.96SHH51 pKa = 6.59LKK53 pKa = 10.64ARR55 pKa = 11.84TFGLLSGLLSHH66 pKa = 6.73GVGGSGSAEE75 pKa = 3.83CEE77 pKa = 3.97ACEE80 pKa = 4.58GEE82 pKa = 4.51AGSSAGGSGSWGGHH96 pKa = 5.19LGGGLSGHH104 pKa = 7.2LGGSLGGSLGLGGSAGSGEE123 pKa = 4.19NCDD126 pKa = 3.7EE127 pKa = 4.32NGEE130 pKa = 4.4GGVSVGGSADD140 pKa = 3.28GSAGSSIGGSIGGSTGGSASGSWGWPVPGSVGGSVGGSASGDD182 pKa = 3.25AGASSSGSGSTEE194 pKa = 3.64GSGSIGGSASGSAGSDD210 pKa = 2.87TGASGTASASGSTEE224 pKa = 3.58GSGSIGGSASGSAGGDD240 pKa = 3.26TSASGTGSASGSTEE254 pKa = 3.67GSGSSSGGISIGGSATGSSGASATASATASASAGASASAGASATGSGPDD303 pKa = 3.44NGEE306 pKa = 4.31DD307 pKa = 3.89CDD309 pKa = 4.0EE310 pKa = 4.57TGADD314 pKa = 3.83ATTGDD319 pKa = 3.66NGDD322 pKa = 5.41DD323 pKa = 3.71YY324 pKa = 11.76GQDD327 pKa = 3.23AGEE330 pKa = 5.03GGDD333 pKa = 3.83SSAGGDD339 pKa = 3.51DD340 pKa = 5.99SEE342 pKa = 6.56GCDD345 pKa = 4.15CDD347 pKa = 3.58EE348 pKa = 4.51

Molecular weight: 30.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4D9T1|A4D9T1_ASPFU Capsule-associated protein CAP1 putative OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=AFUA_1G05595 PE=4 SV=1
MM1 pKa = 7.68LCFQCRR7 pKa = 11.84VVPSALRR14 pKa = 11.84TAMRR18 pKa = 11.84SQPSRR23 pKa = 11.84LQALTLTNSLSTPLRR38 pKa = 11.84TFSSALRR45 pKa = 11.84SQPTPLTQSLPSFRR59 pKa = 11.84TSLTSASSVSFGLTQQTRR77 pKa = 11.84SFSASASLAGKK88 pKa = 9.59RR89 pKa = 11.84ATYY92 pKa = 10.03NPSRR96 pKa = 11.84RR97 pKa = 11.84VQKK100 pKa = 10.2RR101 pKa = 11.84RR102 pKa = 11.84HH103 pKa = 5.33GFLARR108 pKa = 11.84LRR110 pKa = 11.84SRR112 pKa = 11.84GGRR115 pKa = 11.84KK116 pKa = 8.87VLQHH120 pKa = 6.19RR121 pKa = 11.84RR122 pKa = 11.84AKK124 pKa = 9.96GRR126 pKa = 11.84KK127 pKa = 7.78SLSWW131 pKa = 3.4

Molecular weight: 14.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9647

0

9647

4742919

21

8515

491.6

54.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.606 ± 0.021

1.281 ± 0.01

5.558 ± 0.019

6.149 ± 0.028

3.719 ± 0.015

6.699 ± 0.023

2.418 ± 0.011

4.921 ± 0.019

4.628 ± 0.024

9.142 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.119 ± 0.008

3.586 ± 0.013

6.028 ± 0.025

4.051 ± 0.016

6.332 ± 0.021

8.423 ± 0.025

5.886 ± 0.019

6.205 ± 0.018

1.448 ± 0.01

2.8 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski